FastQCFastQC Report
Fri 17 Jun 2016
SRR1527391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188373
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTG11700.6211081205905306No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10920.5797009125511619No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10370.5505035222669916No Hit
CGTGGCGGGATATAAATGAATGAAATATAGATAAACTGTGAGATAAATATCA5620.2983442425400668No Hit
CGTGGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC5450.28931959463405055No Hit
CGTGGCGGGCTGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCA4830.2564061728591677No Hit
CGTGGCGGGATCCCATCAGGCCTCTGACCCAGACTGCTGTGTCCCTTCTTCC4820.25587531121763735No Hit
CGTGGCGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGC4560.24207290853784777No Hit
CGTGGCGGGCTCTTCCACAACCCTCATGTGAACCCACTTCCGACCGGCTATG4460.2367642921225441No Hit
CGTGGCGGGGTTTAGGATGGAGGCCTTTCTGTTGAGTGGAAATGCACCTTAA4410.23410998391489227No Hit
CGTGGCGGAAGGTCTTGGGGTCTCAAGGTGAGTCAGTTTGCTGTCCTGTGAC4330.2298630907826493No Hit
CGTGGCGGACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCATTCG4320.22933222914111895No Hit
CGTGGCGGGAAGTCAACTATGAAGAATTCGTACAGATGATGACTGCAAAATG4320.22933222914111895No Hit
CGTGGCGGGATCTAAAAAAAGGAAAAAAAAGAAAAGAAAGAAAGAAAGAGAG4070.21606068810285975No Hit
CGTGGCGGGACGGAGAAACACCTGATTTCCAGGAAAATCCCCTCAGATGGGC4010.21287551825367756No Hit
CGTGGCGGGATCTTGGCTATCTTTTATCAAAAGTTTTAGCGCCTTCCTTGAT3990.21181379497061684No Hit
CGTGGCGGCAGGGAGATGTGAAATCACTGCCTCTTGTAGCCACTGGGGACCT3940.20915948676296495No Hit
CGTGGCGGCAACACTGTAGGTTCCAAAGGAAATCAGAAAGTGAAGGCAAACG3820.20278914706460058No Hit
CGTGGCGGCACAGAAGATTCAGGCAAGCCCACAGGAGGTAATTCTGGCAAGC3770.2001348388569487No Hit
CGTGGCGGGCAATCTCCCAGGGCTCCCTCCCACCAGCACCTGTGGTGGCTGC3740.19854225393235758No Hit
CGTGGCGGAGTTGGGAGCTGTCTCCTGTGCCTCCCCTCCCTATCCCCAGTCC3650.1937644991585843No Hit
CGTGGCGGGATGCAAATTCTGTTTTATTTTATCCCTAATAACAGAAGTCTCC3590.19057932930940208No Hit
CGTGGCGGGGTGTACCCTGACAGCATCATGTGTGTCAACCTGTGTCAATTAT3530.1873941594602199No Hit
CGTGGCGGGACCCAAAGTTGAAAGCAAAGAGAAAGTGTTAGTGAAAAAAGAC3510.18633243617715914No Hit
CGTGGCGGGCAGCGCTGAGGGACCAGTCTTTCCTGTGACTGCCACCAGCGAA3500.18580157453562876No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.18580157453562876No Hit
CGTGGCGGTAGCAAGTAATGGATGCCACCTGCCCCAGCATTATGGGGGTCAA3490.1852707128940984No Hit
CGTGGCGGAATCGAGGACCCGTAAGAAGGCTGACAGAGCCCCTGTGGCCAAT3450.18314726632797695No Hit
CGTGGCGGGATGTAATCAGTCACCTGGGTGGTGGTGGTGCATGCCTTTATTT3330.17677692662961253No Hit
CGTGGCGGGGCCCTGCCTCTGGTCTAACCCTGTGTACCCTCTGAAGTCACCC3290.17465348006349105No Hit
CGTGGCGGGACCAAGACCTTTTAAAAACCCTTTTATGGCCTCTGTAGGCACC3240.1719991718558392No Hit
CGTGGCGGGATGTAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGTG3230.17146831021430886No Hit
CGTGGCGGGGCGAAAGTCAGCGTACTGTTCAACCTCCCGGCAGTCGGTTTCA3090.16403624723288368No Hit
CGTGGCGGGAGCAAGGGCCAGGTCATGGTGGCACACACCTTTGGTCCCAGCA3090.16403624723288368No Hit
CGTGGCGGGCTCCATGTCTGTGATGTGTCTGGAGTCTCTCGGGACACGACCA3070.16297452394982295No Hit
CGTGGCGGGCTCCAGCTCCATCAGCTGGCCGTGGCAGAGGCCAAGCCCGCTT3010.15978935410064074No Hit
CGTGGCGGGCGAGAAGGGGCCGAAGGCGAAATGAACACAAAGCCTCGAAGCC3000.15925849245911036No Hit
CGTGGCGGGGCCTAAGAAATACTACAGGGGTCATCCTTTTATAAGGACAAAA2970.15766590753451928No Hit
CGTGGCGGGGGTAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTT2950.15660418425145856No Hit
CGTGGCGAGAGAAGTCCTGGGTTCAAATCCCAGCAACCACATGGTGAGTCAC2930.1555424609683978No Hit
CGTGGCGGGGATAAGGACGGCATCACCAACGTACCGCCGGGCACGCGCCAGT2930.1555424609683978No Hit
CGTGGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2900.1539498760438067No Hit
CGTGGCGGCAGCGTTACAGTTTGGGTTTCATTGCTGTGGTATAAGAACAGTG2820.14970298291156375No Hit
CGTGGCGGGGGATGATGCTTCCAGGACAAATCGGCTTCCTCTCCCCACCCTC2810.1491721212700334No Hit
CGTGGCGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAGG2780.14757953634544227No Hit
CGTGGCGGCACCTTTATTAAGAAATTATACTAAACAATGGCGTCCTTGATTG2780.14757953634544227No Hit
CGTGGCGGGATCCTTGCCTCAGCTTCTGATGTCCCCCTTCCTCCCACACAGT2770.14704867470391192No Hit
CGTGGCGGGATCTAGCTTCGTGCTCAGACACGTCTCAACAAGACCTTAGAAC2760.14651781306238154No Hit
CGTGGCGGTATGCAAACTAGCAAATACTTATTAATAAGAAAGTAGCAATCCC2750.1459869514208512No Hit
CGTGGCGGGATCTGGGATACAGTCTAACTCATCTGACTTAATATGCATTTAA2720.14439436649626008No Hit
CGTGGCGGAGATATGGGATGCACATGCACAGGTGCATTGTGGGTAGAGTGCT2710.14386350485472973No Hit
CGTGGCGGGATGAATGAGAAAGGCAAACCAACGATGAGTTGGCTGAGATGGT2680.1422709199301386No Hit
CGTGGCGGGGTCTAGAAGAGTTTGAGCTCCTGGGAAAGGCCTATGGGAAAGC2630.13961661172248677No Hit
CGTGGCGGGGTCCTTGTGTCTGCCCCGTAAAGATGAGCGGGATCTGAGCCTT2620.13908575008095642No Hit
CGTGGCGGACTTATGTCAATGAACAGCTTGCAAGTCCTGTGACACAAGAACT2620.13908575008095642No Hit
CGTGGCGGATCAAACCATTCCTTCTGTAGCTCAGGAGAGCGTCCCTACCCCA2570.13643144187330455No Hit
CGTGGCGGGATTTGGCTTAATGTAATATGTCACTAATTGTCATATATCCTCA2550.13536971859024383No Hit
CGTGGCGGAGTCTATAAAACTTTAAATTAATTCTGTCTTGAAACACAGGAGA2500.13271541038259196No Hit
CGTGGCGGGCTCTACCCTGAAAGCTGAGTTTATTTTCTGAGATTTTCCCTCT2500.13271541038259196No Hit
CGTGGCGGGCTGTGCGGTGCTGTGCTGTGCTGTGCTGTGCAGCTAATCAGAT2460.1305919638164705No Hit
CGTGGCGGGATGAACAGCACCAAGCTCTCGGCTGACACCTACGAGGATCTGA2440.12953024053340978No Hit
CGTGGCGGGATGTGAATGCTGATGCCCTGGAAGACCAGAGATGTCACATCCC2440.12953024053340978No Hit
CGTGGCGGCGGGCAGTGGTGGTGCACACCTTTAGTCTCAGCACTTGGGAGGC2430.1289993788918794No Hit
CGTGGCGGGGTCTTGTGAAAGTGAGATAGCCGTGCTTACTGACCTGGGCTGT2430.1289993788918794No Hit
CGTGGCGGGCAAACAACTCTGTAGAGCTCTCTGCACCCTGTACCCTTTTCAC2420.12846851725034905No Hit
CGTGGCGGGGTGTGGCAGTTTTTAAGTTATTAGTTTTCAAAATCAGTACTTT2410.12793765560881867No Hit
CGTGGCGGGATGGAAGCCAAGCATGCAGTTTCCTTCATGACCTGTACACAGA2380.12634507068422757No Hit
CGTGGCGGTCATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCACA2370.1258142090426972No Hit
CGTGGCGAGGGATGGACAGTTTAGAGGTATAGTCCTCTGCTGTGTTGGACAG2360.12528334740116684No Hit
CGTGGCGGCGTCTTTCTCCCCGGATAGAAAAGGGGGACCTCAGCATATGATG2350.12475248575963646No Hit
CGTGGCGGGAGTTACCATTTTCATTAACAGCATACACCCTTCAATTGTGTTG2330.12369076247657573No Hit
CGTGGCGGCTGTAAATTTATGTGCATGGTGTACGTGCAGATACATGAGAAGG2330.12369076247657573No Hit
CGTGGCGGATTTGAAGATTTGTTTGGTTTTAGTGATTTATTGCCTCAAAAAA2320.12315990083504537No Hit
CGTGGCGGGCAATGGGAGAAATCATGAATCGACTTCTGCCTACTGGTCCTTC2290.12156731591045425No Hit
CGTGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.12156731591045425No Hit
CGTGGCGGGATATAAAGGGAGCCTTAATGGATTTGTTTTCATAATTTAATAT2280.12103645426892388No Hit
CGTGGCGGGAGATGAGTTGAAACGGATTGAGAGAGAACTGGCGGAAGCTCAA2270.12050559262739352No Hit
CGTGGCGAGAGAGAGACTCCTGCTGGTATCAGACTCTCAGGATCTGCCAGGA2260.11997473098586316No Hit
CGTGGCGGGGCCTAGAACATATGTAGTATTCAGACAGTCATCAATGAAGGAT2260.11997473098586316No Hit
CGTGGCGGAGATGAAAAGGTTATAGCATCCTTAACTGAGGCTGTAGGCTGCT2250.11944386934433279No Hit
CGTGGCGGGAGCTGACTTACATGACAAGTACAGTAGTGTATCTGTCCCCTGA2210.11732042277821132No Hit
CGTGGCGGGCTGTATGATATATGAAGAAGAGGAGGAGGAGGAGAGAAGAAGA2200.11678956113668096No Hit
CGTGGCGGCAAGACCAATCCTGTAGTGAAAGTCGGATTCAGTGACCTCTTCC2170.11519697621208984No Hit
CGTGGCGGCCCGCACGGGTGAACCAGTGGCACAGGTACACCAGCATCGCCGG2160.11466611457055947No Hit
CGTGGCGGGGTCAAGGACCCAGGTGGTTCTCTTTCCTCTCTAGGGTCTTTAT2160.11466611457055947No Hit
CGTGGCGGGATACATACATGCATACATACATAGGAATAGTACTCAGCCATAA2140.11360439128749875No Hit
CGTGGCGGGACCCCTGCACGAGGCAAATCATAGTCAGCTGCTGTGGACAGCC2130.11307352964596837No Hit
CGTGGCGGGGGGCACTCCGAGCCGATGCCACCGGCCCGCGGGTCTCTGAAGG2130.11307352964596837No Hit
CGTGGCGGAGGTGCCAGCCGGTATTGCCTTGTATTTCAGGAATGCTCTGGTT2120.112542668004438No Hit
CGTGGCGGGACCTAGTTGAGTGGGGAGGGGGGGGCATCTTCTGACAGCCTGC2120.112542668004438No Hit
CGTGGCGGGGCCTCAATGAACATTGCCTGTTCACCTTATCATGGGTGTACAT2070.10988835979678616No Hit
CGTGGCGGGATCCGATGATTGTTTTGGTTTCTATTCCCTTTCTAACTGCCCA2070.10988835979678616No Hit
CGTGGCGGGGTTAAAGAATAGAACCAAGCCTTGAACTCCTCATCTACCAAGT2050.10882663651372543No Hit
CGTGGCAGGATAAATGGATTTTGAAATGTCTGACCTCACCTGTTAAGTCCCA2030.1077649132306647No Hit
CGTGGCGGGATCCCCTTCCCTTTCTCAGATGTGTGTTCAAAACGAAGGATTG2030.1077649132306647No Hit
CGTGGCGGGAGATAAGTTTTTAATTAAAAAGATTGAGCAGTAAAAAGAATTA2010.10670318994760396No Hit
CGTGGCGGGGAATTCTGATGTCTAGCTGAGAAGCAGCCGGTTCTAGGGAGAA2000.1061723283060736No Hit
CGTGGCGGGAGGTAGACTCTCTGTGATTACATGAGGTGAGGCCAAGGGGGAA1990.10564146666454322No Hit
CGTGGCGGGAAGTTGGTATCATCTTAAGAAACTGCAAGAGAAAAGCTGGGCT1990.10564146666454322No Hit
CGTGGCGGGATGGAGAGAGTCTCACACTTCTGGCTCATGAAGGAGCTGTCTG1990.10564146666454322No Hit
CGTGGCGGGATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTG1970.10457974338148247No Hit
CGTGGCGGAACTGAGTGAGTTCCAGGACAGCCAGGGCTACACTGAGAAACCT1970.10457974338148247No Hit
CGTGGCGGGCTGTTGAAGCTGATGGAAGGGCTTTGACGTGTCATGTTGTTCT1970.10457974338148247No Hit
CGTGGCGGGGTCAAACTGCTCCAGTATTAGTTGCAGGCACTGATGCTTCTGT1970.10457974338148247No Hit
CGTGGCGGGATGAATACTTACGTTTACCTCACTGGTGCATTTAAAATGGACT1950.10351802009842175No Hit
CGTGGCGGGGTTCGTGTGGAACCTGGCGCTAGACCATTCGTAGACGACCTGC1940.10298715845689138No Hit
CGTGGCGGAGTATAAGGATGAAAAAGAAAGCCTTGTTAAGGTTATAAAAGTT1930.10245629681536102No Hit
CGTGGCGGCAAGGAGACCGTAGGCTTCGGAATGCTCAAGGCTAAGGCCCAGG1920.10192543517383065No Hit
CGTGGCGGGATGAAACTCAACTGTGACTCTCGATCAGCCCAGCCCATGTGAC1910.10139457353230029No Hit
CGTGGCGGGCTGGTAGCATGTGCTGAGATCACCTCTTAGGGATGGTGTGACC1890.10033285024923955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC700.046.00000430
TTAGGAT351.0134863E-746.00000412
CGGAAAT351.0134863E-746.0000046
AAATCGG351.0134863E-746.00000428
CGAACGA351.0134863E-746.00000419
AATCATA351.0134863E-746.00000426
TACCAAC551.8189894E-1246.00000439
GCGGTGA351.0134863E-746.0000045
GCGGTCA351.0134863E-746.0000045
GCGGTAC551.8189894E-1246.0000045
TCTTATA351.0134863E-746.00000414
GATCCAA700.046.0000049
GGATCGA351.0134863E-746.0000048
CCTACGA351.0134863E-746.00000438
TATATGA351.0134863E-746.00000418
AAGCGGC351.0134863E-746.00000444
CCGCTTT351.0134863E-746.00000427
GCGTTAC351.0134863E-746.00000411
TTTCACC351.0134863E-746.00000434
TACATCG351.0134863E-746.00000414
Produced by FastQC (version 0.11.5)