FastQCFastQC Report
Fri 17 Jun 2016
SRR1527387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4904
Sequences flagged as poor quality0
Sequence length50
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3637.402120717781403No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCG3056.219412724306688No Hit
ATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA2675.444535073409462No Hit
ATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTG2605.30179445350734No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAAC2054.180261011419249No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1933.935562805872757No Hit
ATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGT1623.303425774877651No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1442.936378466557912No Hit
ATGGCGGATGTAAGTTTTTTTGTACTCAATTCTTTAAGAAATGACAAATT741.5089722675367048No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621.264274061990212No Hit
ATGGCGAGCGCGCGGAACGAAAATCTGTGTGCCTAGCAACAGCTTTCACT470.9584013050570963No Hit
ATGGCGGGGAGTAACTGCAGTTCCAGGGAATCTGATGCCTTCATTCTGGT460.9380097879282219No Hit
ATGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.8564437194127243No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.8564437194127243No Hit
ATGGCGGGGGGGAACACTGCCCCAGCTATGTCACTAGAGGGAGTCTCTGG410.8360522022838499No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT360.734094616639478No Hit
ATGGCGGGGGCTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCCGC270.5505709624796085No Hit
ATGGCGGGGGGTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGGTTTCCT250.5097879282218597No Hit
ATGGCGGGGGGGTTTGTTTGTTTGTTTGTTTTTGTTTTTGTTTTTTTTCT240.48939641109298526No Hit
ATGGCGGATGTAAGTTTTTTTGTACTCAATTCTTTAAGAAATGACAAAAT240.48939641109298526No Hit
ATGGCGGGGGGTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGGTTTTCT230.46900489396411094No Hit
ATGGCGGGGCACGCACTCTGTCACCCTCTTTCCTCATGCTCAGCTGAGAC220.4486133768352365No Hit
ATGGCGGGGGAGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGAACT180.367047308319739No Hit
ATGGCGGGGGAGATGGTGGATACAGAGAATCAGCAGGGGAGAAACTGAGG180.367047308319739No Hit
ATGGCGATTTCAAATATCTTTCAGGCTGCAGAGAAACTAGGTGAGACAGA170.3466557911908646No Hit
ATGGCGGGGCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCACTCTTCC170.3466557911908646No Hit
ATGGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA170.3466557911908646No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGGT170.3466557911908646No Hit
ATGGCGGGGGAGATGGTGAATACAGAGAATCAGCAGGGGAGAAACTGAGG150.30587275693311583No Hit
ATGGCGGGGCGTTGGGATGCATGAACAAGCCAGTCCTGCTGTTCCAAAGC150.30587275693311583No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGGGGGAAAGAAAAAAAAAAAAAA150.30587275693311583No Hit
ATGGCGGGGGCTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGC140.28548123980424145No Hit
ATGGCGGGGGCTCTCTCTCGCTGGCATCTCTCTTGCTCTGCGCGCCCCGC130.26508972267536707No Hit
ATGGCGGGGGGGTTTGTTTGTTTGTTTGTTTTTGTTTTTGTTTTTTTCCT120.24469820554649263No Hit
ATGGCGGGGGCCACATCTCTGTACAGAACATGATGCGTCGCAAGGAGGAA120.24469820554649263No Hit
ATGGCGGGGGGTCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA110.22430668841761825No Hit
ATGGCGGGGCCAGGGGATGTTCTTGAATTCCCTATTATATTGTAAGAATC110.22430668841761825No Hit
ATGGCGGGATGTGTGTGGGTCCATCTCTGATTTTCCCTGTCAGCCTATGG100.20391517128874387No Hit
ATGGCGGGGGGGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGGACT100.20391517128874387No Hit
ATGGCGGGGGTCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA100.20391517128874387No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1835236541598695No Hit
ATGGCGGGGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1835236541598695No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGGGGGAAAAAAAAAAAAAAAAAA90.1835236541598695No Hit
ATGGCGGGGGAGATGGTGGATACAGAGAATCAGCAGGGGAGGAACTGAGG80.1631321370309951No Hit
ATGGCGGGGGGTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGTTTTTCT80.1631321370309951No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAACAC70.14274061990212072No Hit
ATGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTT70.14274061990212072No Hit
ATGGCGGGGGGTGTGAAGATACTATACCTAGTAGTGACTGCAAAGGAAAA70.14274061990212072No Hit
ATGGCGGGGGTCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA70.14274061990212072No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGAAGAAAAAGGAAAAAAAAAAAA70.14274061990212072No Hit
ATGGCGGGGGGGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGAACT70.14274061990212072No Hit
ATGGCGGGGAGCACTGAATTGACCGTGTTGACCATATTTAAGCAGGCATG70.14274061990212072No Hit
ATGGCGGGGGGGGGAGTTGTGTGGGGGCTTTAAGGAACACGTGCAGTCAC60.12234910277324632No Hit
ATGGCGGGGGAGAGAGGGTCAATAAAAATGCTTCTGAGATTAAAAAAAAA60.12234910277324632No Hit
ATGGCGGGGCCAGGGTATGTTCTTGAATTCCCTATTATATTGTAAGAATC60.12234910277324632No Hit
ATGGCGGGGGGAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAA60.12234910277324632No Hit
ATGGCGGGGGATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACC60.12234910277324632No Hit
ATGGCGGGGGCTAAAGTGATAGTACAGATACGGAGTGTCCCTGTCCCCAA60.12234910277324632No Hit
ATGGCGGGGAGTAACTGCAGTTCCAGGGAATCTGATGCCTTCATTCTTGT60.12234910277324632No Hit
ATGGCGGGGGGCACTGAATTGACCGTGTTGACCATATTTAAGCAGGCATG60.12234910277324632No Hit
ATGGCGGATGTAAGTTTTTTTGTACTCCATTCTTTAAGAAATGACAAATT60.12234910277324632No Hit
ATGGCGGGGATCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA60.12234910277324632No Hit
ATGGCGGGGGGTAACACTGCCCCAGCTATGTCACTAGAGGGAGTCTCTGG60.12234910277324632No Hit
ATGGCGGGGGAGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGGACT60.12234910277324632No Hit
ATGGCGGGGAGTAACTGCAGTTCCAGGGGATCTGATGCCTTCATTCTGGT50.10195758564437193No Hit
ATGGCGGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10195758564437193No Hit
ATGGCGGGGGATCGGAGAAGTATCTGGGGAAGGAGTACGTCATAGCGACC50.10195758564437193No Hit
ATGGCGGGGAATACAAGAGTTACAATGAATTTTCCTTTTGATGTTAATTG50.10195758564437193No Hit
ATGGCGGGGGACTGAGAAGTTATACATAAAAAAATTTGGATAACAAAAGT50.10195758564437193No Hit
ATGGCGGGGACTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCCGC50.10195758564437193No Hit
ATGGCGGGGGCCACATCTCTGTACAGAACATGATGCGTCGCAAGGAGGGA50.10195758564437193No Hit
ATGGCGGGGGTCAGGAAGGGGCGAAAGATATGTGGGATAAACTGAGAAAA50.10195758564437193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC253.8259983E-544.04
CGTTTAC351.0921758E-744.040
CTACAAG405.7880243E-944.014
CATGATT302.0491952E-644.025
CAAGAGC351.0921758E-744.014
AATCCTT405.7880243E-944.021
GGGTTTC253.8259983E-544.042
CAAGAAT405.7880243E-944.017
TCCTTCT405.7880243E-944.023
GAGAAAT302.0491952E-644.06
AAACTCC302.0491952E-644.032
CAGTTTT405.7880243E-944.031
TAAACTC302.0491952E-644.031
CAGCAAT351.0921758E-744.035
CAGCAAG351.0921758E-744.011
TTACCTT351.0921758E-744.043
CCAGTTT405.7880243E-944.030
GCAAGAG351.0921758E-744.013
TTCGCGT351.0921758E-744.036
GAAACAT302.0491952E-644.021
Produced by FastQC (version 0.11.5)