FastQCFastQC Report
Fri 17 Jun 2016
SRR1527386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4187.654275773667826No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGA2294.193371177440029No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCT2194.010254532136972No Hit
ATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCG1683.076359641091375No Hit
ATGGCGGATGTAAGTTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTT1632.984801318439846No Hit
ATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAACA1452.6551913568943415No Hit
ATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCG1352.472074711591284No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1342.4537630470609777No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGGTTTCCTTG1322.4171397180003664No Hit
ATGGCGGGGAGTAACTGCAGTTCCAGGGAATCTGATGCCTTCATTCTGGTCT1272.3255813953488373No Hit
ATGGCGGGGCCAGGGTATGTTCTTGAATTCCCTATTATATTGTAAGAATCTA891.6297381431972167No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA821.501556491485076No Hit
ATGGCGGGATTTGCCGGGTTCCCAAAAGCAAGCTGGAAGTGGGTCGCATCAT641.1719465299395715No Hit
ATGGCGGGGAATACAAGAGTTACAATGAATTTTCCTTTTGATGTTAATTGCC641.1719465299395715No Hit
ATGGCGGGGGGTAACACTGCCCCAGCTATGTCACTAGAGGGAGTCTCTGGCC601.0986998718183483No Hit
ATGGCGAGCGCGCGGAACGAAAATCTGTGTGCCTAGCAACAGCTTTCACTTC561.025453213697125No Hit
ATGGCGGGGGCCAAAGATAAACAACCGTATGAGCAGAAAGCAGCTAAACTAA490.8972715619849845No Hit
ATGGCGGGGGAGACGGGGTTTCTCTGTATAGCCTTGGCTGTCCTGGAACTCA460.842336568394067No Hit
ATGGCGGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAAC450.8240249038637613No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.7874015748031495No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG430.7874015748031495No Hit
ATGGCGGGGAACCGGGATTGGCAGAATTACTACTACCACCACCAACAGGACA330.6042849295000916No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.6042849295000916No Hit
ATGGCGGGGATCAGGAAGGGGCGAAAGATATGTGGGATAAACTGAGAAAAGA320.5859732649697857No Hit
ATGGCGGGGGAGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGAACTCA320.5859732649697857No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCCGTTTTCTGGGGGTTTCCTTG310.5676616004394799No Hit
ATGGCGGGGAGCACTGAATTGACCGTGTTGACCATATTTAAGCAGGCATGGT290.5310382713788684No Hit
ATGGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA290.5310382713788684No Hit
ATGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.5310382713788684No Hit
ATGGCGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGGTGTACGTGCTCCAGA280.5127266068485625No Hit
ATGGCGGGGGGTGTGAAGATACTATACCTAGTAGTGACTGCAAAGGAAAATG270.4944149423182567No Hit
ATGGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.4944149423182567No Hit
ATGGCGGGGCGTTGGGATGCATGAACAAGCCAGTCCTGCTGTTCCAAAGCTG270.4944149423182567No Hit
ATGGCGGGGCACGCACTCTGTCACCCTCTTTCCTCATGCTCAGCTGAGACAT260.4761032777879509No Hit
ATGGCGGGGCCAGGGGATGTTCTTGAATTCCCTATTATATTGTAAGAATCTA260.4761032777879509No Hit
ATGGCGGGGACTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGC260.4761032777879509No Hit
ATGGCGGGGGTTGACTTCTACCTTGATGTTATTTTTAATTGTTTTCAATTTT240.4394799487273393No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.4394799487273393No Hit
ATGGCGGGGACTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCCGCGC240.4394799487273393No Hit
ATGGCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC230.4211682841970335No Hit
ATGGCGGGGAACTGAGAAGTTATACATAAAAAAATTTGGATAACAAAGGTTC220.4028566196667277No Hit
ATGGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCCTGACCCGCC210.3845449551364219No Hit
ATGGCGGGGACTAAAGTGATAGTACAGATACGGAGTGTCCCTGTCCCCAATG210.3845449551364219No Hit
ATGGCGGGGCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCACTCTTCCAG210.3845449551364219No Hit
ATGGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.3662332906061161No Hit
ATGGCGATTTCAAATATCTTTCAGGCTGCAGAGAAACTAGGTGAGACAGAAA200.3662332906061161No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGGGGGAAAGAAAAAAAAAAAAAAAA190.3479216260758103No Hit
ATGGCGGGGGGTAACACTGCCCCCGCTATGTCACTAGAGGGAGTCTCTGGCC170.3112982970151987No Hit
ATGGCGGGGGACATCCAGAGCCCTCAGTTCTTTTTACACAACATTGTCTGAT170.3112982970151987No Hit
ATGGCGGGGGATCTGTGACTCTTTTGCCTTGAAGAGCAAGAAGATGGCCAAC150.27467496795458707No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGTTTTCCTTG150.27467496795458707No Hit
ATGGCGGGGGTGGGCAAAGCTCTGGATCTTATCCCAAGAGCTGCCAAAACAA140.25636330342428126No Hit
ATGGCGGGGCTCTCTTTCCGTCTCCGGCCGCCCTAGGGAGAGGAGCCGCCGC130.23805163889397546No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGAAGAAAAAGGAAAAAAAAAAAAAA110.20142830983336385No Hit
ATGGCGGGATTTGCCGGGTTCCCAAAAGCAAGCTGGAAGTGGGGCGCATCAT110.20142830983336385No Hit
ATGGCGGGGAACCTGAGTGTCCTGTGTCTCCTGTATCGGCAGGTGGGTTTTT110.20142830983336385No Hit
ATGGCGGGGTATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGA110.20142830983336385No Hit
ATGGCGGGATGTGTGTGGGTCCATCTCTGATTTTCCCTGTCAGCCTATGGAG110.20142830983336385No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGGGGGAAAAAAAAAAAAAAAAAAAA100.18311664530305805No Hit
ATGGCGGGGACCACATCTCTGTACAGAACATGATGCGTCGCAAGGAGGAAGA100.18311664530305805No Hit
ATGGCGGGGATCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA100.18311664530305805No Hit
ATGGCGGGGAGTCCTCCCCAAAACATGGTCAGGTTGTCACAGGAATACCCCA100.18311664530305805No Hit
ATGGCGGGGATTCACAGCTGGGCCTCCCGGGCCAGTTGCAGGGAGAATGAAT100.18311664530305805No Hit
ATGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.18311664530305805No Hit
ATGGCGGGGGACTGAGAAGTTATACATAAAAAAATTTGGATAACAAAGGTTC90.16480498077275224No Hit
ATGGCGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCCAGA90.16480498077275224No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG80.14649331624244644No Hit
ATGGCGGGGGATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGA80.14649331624244644No Hit
ATGGCGGGGGGTGGAGTTGTGTGGGGTCTTTAAGGAACACGTGCAGTCACCT80.14649331624244644No Hit
ATGGCGGGGATCTCAGGATGCCGAACATGAGCTTCTCTTTGACCTCATTGGG80.14649331624244644No Hit
ATGGCGGGGGGGGGAATGGCATGGCCTTCAGCAAGGACATCTCCATATGGAA70.12818165171214063No Hit
ATGGCGGGGGAAGGCTTTGGTCTCCCTGGGGGGGGGGTGAGGGGTTGAGGCA70.12818165171214063No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGGTTTTCTTG70.12818165171214063No Hit
ATGGCGGGGAAGATGGTGAATACAGAGAATCAGCAGGGGAGAAACTGAGGTG70.12818165171214063No Hit
ATGGCGGGGGCAGGGGCAGTGACTCGCCCGTGCATCGTCCAAAAAAAAAAAA70.12818165171214063No Hit
ATGGCGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGGTGTACGTGCTCCCGA60.10986998718183483No Hit
ATGGCGGGGGGCACTGAATTGACCGTGTTGACCATATTTAAGCAGGCATGGT60.10986998718183483No Hit
ATGGCGGGGAACCCTAGACAGGAGGAGGTTAGAGAGTCTGTCTTGATTTGTG60.10986998718183483No Hit
ATGGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC60.10986998718183483No Hit
ATGGCGGGGATTCAAAAAGATAGTTAAATCATGCTACTGAGCCTATGATGTC60.10986998718183483No Hit
ATGGCGGGGGGTGGAGTTGTGTGGGGGCTTTAAGGAACACGTGCAGTCACCT60.10986998718183483No Hit
ATGGCGGGGATTCACAGCTGGGCCTCCCGGGCCCGTTGCAGGGAGAATGAAT60.10986998718183483No Hit
ATGGCGGGGGGTAACACTGCCCCAGCTATGTCACTAGAGGGGGTCTCTGGCC60.10986998718183483No Hit
ATGGCGGGGGGGGGAGTTGTGTGGGGGCTTTAAGGAACACGTGCAGTCACCT60.10986998718183483No Hit
ATGGCGGGGGTTACTGTGTATGCAGGAACCCAGCTTTGTCTGCTGCATGAAA60.10986998718183483No Hit
ATGGCGACTGTGGGGCCTATTTCTGTTGCTATGGACGCAAGCCATCCGTCTC60.10986998718183483No Hit
ATGGCGGGGGGTTTTGTTTGTTTGTTTGTTTTTGTTTTTGTTTTTTTCCTAC60.10986998718183483No Hit
ATGGCGGGGAATCTTAGCTCTGGGGTGACTACAGCTTAGAAAAAGCTCTTGG60.10986998718183483No Hit
ATGGCGGGGGAGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGGACTCA60.10986998718183483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA1150.046.0000041
TTTTGTA252.987774E-546.018
ACAAATT252.987774E-546.044
CGCTGGC205.7721656E-446.019
GCGGGGA900.046.04
GGATGTA252.987774E-546.06
CTACAAG252.987774E-546.014
CATGATT404.0545274E-946.025
TGGCATC205.7721656E-446.022
AATCCTT252.987774E-546.021
CAAGAAT252.987774E-546.017
TCCTTCT252.987774E-546.023
GAGAAAT404.0545274E-946.06
GGTTGCT252.987774E-546.09
ATCTCTC205.7721656E-446.026
TAAACTC404.0545274E-946.031
GGGTTGC252.987774E-546.08
CAGTCGG452.055458E-1046.033
GGCATCT205.7721656E-446.023
TAAACAT452.055458E-1046.020
Produced by FastQC (version 0.11.5)