FastQCFastQC Report
Fri 17 Jun 2016
SRR1527385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6226
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4146.649534211371668No Hit
ATGGCGGATGTAAGTTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTT2013.228397044651462No Hit
ATGGCGGGGAGTAACTGCAGTTCCAGGGAATCTGATGCCTTCATTCTGGTCT1772.8429168005139736No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1622.6019916479280436No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGA1382.216511403790556No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCT1322.1201413427561837No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGGTTTCCTTG1211.9434628975265018No Hit
ATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCG1171.8792161901702538No Hit
ATGGCGGGGGGTAACACTGCCCCAGCTATGTCACTAGAGGGAGTCTCTGGCC1101.76678445229682No Hit
ATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAACA1021.6382910375843238No Hit
ATGGCGGGGCCAGGGTATGTTCTTGAATTCCCTATTATATTGTAAGAATCTA1021.6382910375843238No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA891.429489238676518No Hit
ATGGCGGGATTTGCCGGGTTCCCAAAAGCAAGCTGGAAGTGGGTCGCATCAT881.4134275618374559No Hit
ATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCG801.2849341471249598No Hit
ATGGCGAGCGCGCGGAACGAAAATCTGTGTGCCTAGCAACAGCTTTCACTTC771.2367491166077738No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG751.2046257629296497No Hit
ATGGCGGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAAC721.156440732412464No Hit
ATGGCGGGGAACCGGGATTGGCAGAATTACTACTACCACCACCAACAGGACA641.027947317699968No Hit
ATGGCGGGGGAGACGGGGTTTCTCTGTATAGCCTTGGCTGTCCTGGAACTCA620.9958239640218438No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.9155155798265339No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCAGTTTTCTGGGGTTTTCCTTG560.8994539029874719No Hit
ATGGCGGGGAATACAAGAGTTACAATGAATTTTCCTTTTGATGTTAATTGCC540.8673305493093478No Hit
ATGGCGGGGCACGCACTCTGTCACCCTCTTTCCTCATGCTCAGCTGAGACAT510.8191455187921619No Hit
ATGGCGGGGAGCACTGAATTGACCGTGTTGACCATATTTAAGCAGGCATGGT500.8030838419530999No Hit
ATGGCGGGGCGTTGGGATGCATGAACAAGCCAGTCCTGCTGTTCCAAAGCTG440.7067137809187279No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.7067137809187279No Hit
ATGGCGGGGCCAGGGGATGTTCTTGAATTCCCTATTATATTGTAAGAATCTA420.6745904272406039No Hit
ATGGCGGGGATCAGGAAGGGGCGAAAGATATGTGGGATAAACTGAGAAAAGA410.6585287504015419No Hit
ATGGCGGGGGCCAAAGATAAACAACCGTATGAGCAGAAAGCAGCTAAACTAA410.6585287504015419No Hit
ATGGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA410.6585287504015419No Hit
ATGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.6585287504015419No Hit
ATGGCGGGGACTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGC400.6424670735624799No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.610343719884356No Hit
ATGGCGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGGTGTACGTGCTCCAGA380.610343719884356No Hit
ATGGCGGGGGAGACGGGGGTTCTCTGTATAGCCTTGGCTGTCCTGGAACTCA360.578220366206232No Hit
ATGGCGGGGCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCACTCTTCCAG320.513973658849984No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGGGGGAAAGAAAAAAAAAAAAAAAA320.513973658849984No Hit
ATGGCGGGGACTAAAGTGATAGTACAGATACGGAGTGTCCCTGTCCCCAATG220.35335689045936397No Hit
ATGGCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC220.35335689045936397No Hit
ATGGCGGGGACCACATCTCTGTACAGAACATGATGCGTCGCAAGGAGGAAGA210.33729521362030196No Hit
ATGGCGATTTCAAATATCTTTCAGGCTGCAGAGAAACTAGGTGAGACAGAAA210.33729521362030196No Hit
ATGGCGGGGGAAGGCTTTGGTCTCCCTGGGAGGGGGGTGAGGTGTTGAGGCA200.32123353678123995No Hit
ATGGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.32123353678123995No Hit
ATGGCGGGGGGTGTGAAGATACTATACCTAGTAGTGACTGCAAAGGAAAATG190.305171859942178No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCCGTTTTCTGGGGGTTTCCTTG190.305171859942178No Hit
ATGGCGGGGGTGGGCAAAGCTCTGGATCTTATCCCAAGAGCTGCCAAAACAA190.305171859942178No Hit
ATGGCGGGGGTTGACTTCTACCTTGATGTTATTTTTAATTGTTTTCAATTTT180.289110183103116No Hit
ATGGCGGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGAT180.289110183103116No Hit
ATGGCGGGATGTGTGTGGGTCCATCTCTGATTTTCCCTGTCAGCCTATGGAG180.289110183103116No Hit
ATGGCGGGGGGGACGTTGGAGCCGAGGGCGGACCTGCTGCCCCAGAAGCACT170.27304850626405397No Hit
ATGGCGGGGGAGTGCAGTGCTATGGCGGAAGAAAAAGGAAAAAAAAAAAAAA160.256986829424992No Hit
ATGGCGGGGGACATCCAGAGCCCTCAGTTCTTTTTACACAACATTGTCTGAT160.256986829424992No Hit
ATGGCGGGGAACTGAGAAGTTATACATAAAAAAATTTGGATAACAAAGGTTC150.24092515258592995No Hit
ATGGCGGGGAGTCCTCCCCAAAACATGGTCAGGTTGTCACAGGAATACCCCA150.24092515258592995No Hit
ATGGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.22486347574686796No Hit
ATGGCGGGGAATCTTAGCTCTGGGATGACTACAGCTTAGAAAAAGCTCTTGG140.22486347574686796No Hit
ATGGCGGGGGGTAACACTGCCCCCGCTATGTCACTAGAGGGAGTCTCTGGCC140.22486347574686796No Hit
ATGGCGGGGAACTGAGAAGTTATACATAAAAAAATTTGGATAACAAAGGGTC130.20880179890780595No Hit
ATGGCGGGGACTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCCGCGC130.20880179890780595No Hit
ATGGCGGGGGATGCCGACTGGCGATGCGGCGGCGGTATTCCCATGACCCGCC130.20880179890780595No Hit
ATGGCGGGGAACCTGAGTGTCCTGTGTCTCCTGTATCGGCAGATGGGTTCTT120.19274012206874397No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG110.17667844522968199No Hit
ATGGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC110.17667844522968199No Hit
ATGGCGGGGGGTGGAGTTGTGTGGGGGCTTTAAGGAACACGTGCAGTCACCT110.17667844522968199No Hit
ATGGCGGGGATTCACAGCTGGGCCTCCCGGGCCAGTTGCAGGTAGAATGAAT100.16061676839061997No Hit
ATGGCGGGGATCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA100.16061676839061997No Hit
ATGGCGGGGAAGATGGTGAATACAGAGAATCAGCAGGGGAGAAACTGAGGTG90.144555091551558No Hit
ATGGCGGGGGTTGCTACAAGAATCCTTCTCCCGTTTTCTGGGGTTTTCCTTG90.144555091551558No Hit
ATGGCGGGGTGTGTGAAGATACTATACCTAGTAGTGACTGCAAAGGAAAATG90.144555091551558No Hit
ATGGCGGGGGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.144555091551558No Hit
ATGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.144555091551558No Hit
ATGGCGGGGTATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGA80.128493414712496No Hit
ATGGCGGGGGATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGA80.128493414712496No Hit
ATGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGC80.128493414712496No Hit
ATGGCGGGATCAGGGGCAGGCATCACTGGTTATAAAAAGAACCCAACCTCTA80.128493414712496No Hit
ATGGCGGGGGGTGGAGTTGTGTGGGGTCTTTAAGGAACACGTGCAGTCACCT80.128493414712496No Hit
ATGGCGGGGGGGTTTGTTTGTTTGTTTGTTTTTGTTTTTGTTTTTTTCCTAC80.128493414712496No Hit
ATGGCGGGGCTCTCTTTCCGTCTCCGGCCGCCCTAGGGAGAGGAGCCGCCGC70.11243173787343398No Hit
ATGGCGGGGAACCCTAGACAGGAGGAGGGTAGAGAGTCTGTCTTGATTTGTG70.11243173787343398No Hit
ATGGCGGGGACTTGCACAACGAGCACTTTCACTGTGGCAGTCTCACCAATCT70.11243173787343398No Hit
ATGGCGGGGGATCTGTGACTCTTTTGCCTTGAAGAGCAAGAAGATGGCCAAC70.11243173787343398No Hit
ATGGCGGGGAACCTGAGTGTCCTGTGTCTCCTGTATCGGCAGATGGATTCTT70.11243173787343398No Hit
ATGGCGGGGATTCAAAAAGATAGTTAAATCATGCTACTGAGCCTATGATGTC70.11243173787343398No Hit
ATGGCGGGGATCCATGACCTAACTCAGATCCACTCAGACATGAACCTCCCAG70.11243173787343398No Hit
ATGGCGGGGGCTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGC70.11243173787343398No Hit
ATGGCGGGGCATTAGAACTTAATATTAATTTTGGTGATCTGATATTCTTCCA70.11243173787343398No Hit
ATGGCGGGGGAACCTCTCTTATAGGCCTCACAAGGTGATCTGACCACACCCC70.11243173787343398No Hit
ATGGCGGGGAGCTAGGAAGCATGGAAAAACCAATATGGAGGTTGGCCAGTGA70.11243173787343398No Hit
ATGGCGGGGGGTAACTGCAGTTCCAGGGAATCTGATGCCTTCATTCTGGTCT70.11243173787343398No Hit
ATGGCGGGGGTTACTGTGTATGCAGGAACCCAGCTTTGTCTGCTGCATGAAA70.11243173787343398No Hit
ATGGCGGGGGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATT70.11243173787343398No Hit
ATGGCGGGGGGTTTTGTTTGTTTGTTTGTTTTTGTTTTTGTTTTTTTCCTAC70.11243173787343398No Hit
ATGGCGGGGGAAGGCTTTGGTCTCCCTGGGGGGGGGGTGAGGTGTTGAGGCA70.11243173787343398No Hit
ATGGCGGGGGTACACATGCGCGCGCGCACACACACACACACACTTACACACA70.11243173787343398No Hit
ATGGCGGGGGGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11243173787343398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA750.046.0000041
TTTTGTA301.5780624E-646.018
ACAAATT301.5780624E-646.044
GCGGGGC301.5780624E-646.04
GCGGGGA1150.046.04
GGATGTA301.5780624E-646.06
ACTGCCC205.835979E-446.016
ACCGGGA205.835979E-446.011
GGGTTTC253.0377169E-546.042
GTCACTA205.835979E-446.030
TGGCAGA205.835979E-446.019
CCAACAG205.835979E-446.042
AACCGGG205.835979E-446.010
CAGTCGG253.0377169E-546.033
TAAACAT253.0377169E-546.020
AAATTTT301.5780624E-646.046
CTTTAAG301.5780624E-646.032
GAAATGA301.5780624E-646.038
ATCCAAA253.0377169E-546.010
AATTCTT301.5780624E-646.028
Produced by FastQC (version 0.11.5)