FastQCFastQC Report
Fri 17 Jun 2016
SRR1527384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26700
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC25769.647940074906366No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT17296.47565543071161No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA16586.209737827715356No Hit
CCGACGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG11174.1835205992509366No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGA6752.528089887640449No Hit
CCGACGGGGCGGGCATTCAACAAACAGTAATAGCGCACATGCACTGCCAG4981.8651685393258428No Hit
CCGACGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAG3261.2209737827715357No Hit
CCGACGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG2751.0299625468164793No Hit
CCGACGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGT2500.9363295880149813No Hit
CCGACGGGGGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA2330.8726591760299626No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC2270.8501872659176031No Hit
CCGACGGGCAGAGGTTTCCAAACTACTTGAAGGTCTTTTAAAAATGTTCC2190.8202247191011236No Hit
CCGACGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC1770.6629213483146068No Hit
CCGACGGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA1570.5880149812734082No Hit
CCGACGGGGATGGAATTATAAATTTCCCCAATTCCTTTCATGCGACAAAG1570.5880149812734082No Hit
CCGACGGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAA1520.5692883895131086No Hit
CCGACGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCC1350.5056179775280899No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG1300.48689138576779023Illumina Single End Adapter 2 (100% over 23bp)
CCGACGGGGTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCGACCC1220.45692883895131087No Hit
CCGACGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAG1140.4269662921348315No Hit
CCGACGGGGGATAAAGACATTTGAAAATCTGTCTTCTGACAAATAAAAAG1120.4194756554307116No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG1110.4157303370786517No Hit
CCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAA1100.41198501872659177No Hit
CCGACGGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC1090.40823970037453183No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTG1020.3820224719101124No Hit
CCGACGGGGAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACG970.36329588014981273No Hit
CCGACGGGTGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG970.36329588014981273No Hit
CCGACGGGGGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGA940.35205992509363293No Hit
CCGACGGACTAACACTAAGTGTATACTCTTGTTTTTCATCAACCATTTAA940.35205992509363293No Hit
CCGACGGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT900.33707865168539325No Hit
CCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATG870.3258426966292135No Hit
CCGACGGGGGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTT850.31835205992509363No Hit
CCGACGGGATCTTCCCTGGCCCCTGGGTCTCTTTCCTGTGGGAGTGTAAC820.30711610486891383No Hit
CCGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG810.30337078651685395No Hit
CCGACGGGGCCTTCCAGGCGACCATTCCGGATGTGCTGCCCAAGGAGGGG800.299625468164794No Hit
CCGACGGGGCAGCTCTATTTCGGCGCGCTGGTCGCGGGGCTCCGGGCCGG790.2958801498127341No Hit
CCGACGGGGGTAAATAATGGATTGTGTTTTATTTGTGCAATATTATTTAT790.2958801498127341No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCACA750.2808988764044944No Hit
CCGACGGGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCT750.2808988764044944No Hit
CCGACGAGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGT740.27715355805243447No Hit
CCGACGGGGGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT720.2696629213483146No Hit
CCGACGGGGGTCTGGCATATTTTGGACTCAATTAATGTCCACTGGAGATT720.2696629213483146No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACACCA660.24719101123595505No Hit
CCGACGGGGGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATCCGCTTCC650.24344569288389512No Hit
CCGACGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC640.2397003745318352No Hit
CCGACGGGGCACTGACCTGAACGATACCGAATCTGACTCCACTACGCTGG640.2397003745318352No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGC620.23220973782771534No Hit
CCGACGGGATGCGCACCGAGGGCGGCCTGCTCGTTGCAGAGACGGCCAAG610.22846441947565543No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGCGA600.22471910112359553No Hit
CCGACGGGAGTAATGGCAGCATTTGTCTTGATATTCTAAGACCACAGTGG590.22097378277153557No Hit
CCGACGGGGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCTG560.2097378277153558No Hit
CCGACGGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGG550.20599250936329588No Hit
CCGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG550.20599250936329588No Hit
CCGACGGGGCGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGA550.20599250936329588No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGAGA530.19850187265917602No Hit
CCGACGGGGCTTCCTCGAGGTCACAGCAGTGCTGTCTGAGACATTCGGTG530.19850187265917602No Hit
CCGACGGGGTGCGGAAGTGCCCTCCTTTCCTTTCTGCTGGGTGCCGGCTG530.19850187265917602No Hit
CCGACGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGAC520.1947565543071161No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC520.1947565543071161Illumina Single End Adapter 2 (95% over 22bp)
CCGACGGGGAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTT510.1910112359550562No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGACGGGGA500.18726591760299627No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCT500.18726591760299627No Hit
CCGACGGGGGTCCTGGCTATCCAATAGCATCGAGTGGAGCATCCCGGGAA500.18726591760299627No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGG490.18352059925093633No Hit
CCGACGGGGGAAAGGCCAGACTTGTAGGCATTGATAGGGCAGAGTGCTGA490.18352059925093633No Hit
CCGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGAAG480.1797752808988764No Hit
CCGACGGGGGGGAATTCCCTCGATTCTGTTTTGCTACCCGTTGTAGCGCC480.1797752808988764No Hit
CCGACGGGAGTTGTGTTTGACTCACAACCCCAGAAACAGACATCATGGTG480.1797752808988764No Hit
CCGACGGGACGCCCACCTACGGGGACCTCAACCACCTCGTGTCAGCCACC470.17602996254681647No Hit
CCGACGAGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC460.17228464419475653No Hit
CCGACGGGTGGCCCTGAGGCCAGCCTTGCCCAGCTCATTAGCGCCTTGCG450.16853932584269662No Hit
CCGACGGGAGAGATCAACCCGTACCTTCTGGGCACCATGGCTGGGGGTGC440.1647940074906367No Hit
CCGACGGGCTCCAGACGGGATCAGATCAAAACCAACCCGGTGAGCTCCCT430.16104868913857678No Hit
CCGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA430.16104868913857678No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACTGGGA430.16104868913857678No Hit
CCGACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT410.15355805243445692No Hit
CCGACGGGGGATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA410.15355805243445692No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTC390.14606741573033707No Hit
CCGACGGGGTCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGG390.14606741573033707No Hit
CCGACGGGAGCTCTGCTCTGACAGCCTTGGGTGCCTGTGCGGTGGGCGGG380.14232209737827714No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTTGACTCACAACCCC370.13857677902621723No Hit
CCGACGGGGATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGG350.1310861423220974No Hit
CCGACGGGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAA350.1310861423220974No Hit
CCGACGGGGAGGAGTTAGGCGCGATGCTTTCCACTGCTGCTTACCGAGAC350.1310861423220974No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA350.1310861423220974No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGCAGAGCTCG340.12734082397003746No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC340.12734082397003746No Hit
CCGACGGGAGTAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTAT340.12734082397003746No Hit
CCGACGGGGGACGATACCGATAAGTATCATGCGATACTCAATGGTATCGC330.12359550561797752No Hit
CCGACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT330.12359550561797752No Hit
CCGACGGGGGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGAAAGGTGAA330.12359550561797752No Hit
CCGACGGGGTTTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGC330.12359550561797752No Hit
CCGACGGGGGACTGGAGATTTCTCAAAGAACTTAAAACAGAGATATCATT330.12359550561797752No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT330.12359550561797752No Hit
CCGACGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCACAG330.12359550561797752No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGAGGA320.1198501872659176No Hit
CCGACGGGGGTCTTCTGACAAATAAAAAGCATTTATTTCACTGCAAAAAA310.11610486891385767No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC310.11610486891385767Illumina Single End Adapter 2 (100% over 21bp)
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCGCATGGC310.11610486891385767No Hit
CCGACGGGGGAGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC310.11610486891385767No Hit
CCGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC300.11235955056179776No Hit
CCGACGGGGAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATC300.11235955056179776No Hit
CCGACGGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGA300.11235955056179776No Hit
CCGACGGGGCCTGTCCCGTCGCGACCATGTCGTTCTGCAGCTTCTTCGGA290.10861423220973783No Hit
CCGACGGCGCCGAGGTGCTATCAGTGCTGAAGTTTACACTGAGGAGGATG290.10861423220973783No Hit
CCGACGGGGCTCTTCGGGCTTAGCCGCCGCGCTGCCCGGCTGCTCCGTCC290.10861423220973783No Hit
CCGACGGGGGCCTGGATGCTGCCGGAAAAAGCCGAGGACGATACCCGCCC280.1048689138576779No Hit
CCGACGGGGCATTGAAGAAGAAACCTCGGGGAGGTCTGGAGCTGTCTTTC280.1048689138576779No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCTTCGTATGCCG280.1048689138576779No Hit
CCGACGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA280.1048689138576779Illumina Single End Adapter 2 (100% over 34bp)
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGCAGCGCTCG280.1048689138576779No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACGGGGA280.1048689138576779No Hit
CCGACGGGGAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTC270.10112359550561799No Hit
CCGACGGGCTCCAGACGGGATCAGGTCAAAACCAACCCGGTGAGCTCCCT270.10112359550561799No Hit
CCGACGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA270.10112359550561799Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCT207.7189686E-444.010
CGCTGGT207.7189686E-444.026
CTTTCAT351.3763565E-744.035
CTTTTAG207.7189686E-444.033
GGATGTT254.3267537E-544.035
AGCCCAT207.7189686E-444.023
GCGGGCA551.8189894E-1244.09
TGTGACA302.4357523E-644.043
TTTGAAA254.3267537E-544.020
TGCACTG551.8189894E-1244.040
TAGCGCC207.7189686E-444.044
TAGCGCA551.8189894E-1244.031
ACTGTGA302.4357523E-644.041
TTTTGCT254.3267537E-544.029
TGGTCGC207.7189686E-444.029
ACTGCCA551.8189894E-1244.043
GGAGACA207.7189686E-444.08
CATGATA207.7189686E-444.041
ACAAACA551.8189894E-1244.020
CCAATTC351.3763565E-744.028
Produced by FastQC (version 0.11.5)