FastQCFastQC Report
Fri 17 Jun 2016
SRR1527383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20328
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC20039.853404171585991No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA15007.378984651711924No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT14026.896890987800079No Hit
CCGACGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA10525.17512790240063No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG4312.120228256591893No Hit
CCGACGGGGCGGGCATTCAACAAACAGTAATAGCGCACATGCACTGCCAGCT4182.0562770562770565No Hit
CCGACGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGAA2611.2839433293978748No Hit
CCGACGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT2321.1412829594647778No Hit
CCGACGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC2231.0970090515545061No Hit
CCGACGGGGGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAAC2181.0724124360487997No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG1720.8461235733963006No Hit
CCGACGGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA1630.8018496654860291No Hit
CCGACGGGCAGAGGTTTCCAAACTACTTGAAGGTCTTTTAAAAATGTTCCAG1460.7182211727666272No Hit
CCGACGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG1300.6395120031483668No Hit
CCGACGGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGC1230.6050767414403778No Hit
CCGACGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGC1200.5903187721369539No Hit
CCGACGGGGGATAAAGACATTTGAAAATCTGTCTTCTGACAAATAAAAAGCA1190.5853994490358126No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1190.5853994490358126Illumina Single End Adapter 2 (100% over 25bp)
CCGACGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC1140.5608028335301062No Hit
CCGACGGGGATGGAATTATAAATTTCCCCAATTCCTTTCATGCGACAAAGGA1050.5165289256198348No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGGAG930.45749704840613925No Hit
CCGACGGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA880.4329004329004329No Hit
CCGACGGGGGTAAATAATGGATTGTGTTTTATTTGTGCAATATTATTTATTA870.42798110979929166No Hit
CCGACGGGGTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCGACCCAC860.4230617866981503No Hit
CCGACGGGGAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGAC840.4132231404958678No Hit
CCGACGGGATCTTCCCTGGCCCCTGGGTCTCTTTCCTGTGGGAGTGTAACTG840.4132231404958678No Hit
CCGACGGACTAACACTAAGTGTATACTCTTGTTTTTCATCAACCATTTAAGA840.4132231404958678No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGGCGGGGAAG820.4033844942935852No Hit
CCGACGGGTGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGG780.3837072018890201No Hit
CCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCG760.3738685556867375No Hit
CCGACGGGGGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG750.36894923258559625No Hit
CCGACGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC730.3591105863833136No Hit
CCGACGGGGCAGCTCTATTTCGGCGCGCTGGTCGCGGGGCTCCGGGCCGGGC700.34435261707988984No Hit
CCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAG690.3394332939787485No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTGAA680.33451397087760726No Hit
CCGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCG670.32959464777646597No Hit
CCGACGGGGCCTTCCAGGCGACCATTCCGGATGTGCTGCCCAAGGAGGGGGA670.32959464777646597No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA630.30991735537190085No Hit
CCGACGGGGGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCC620.30499803227075956No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGGCGGGGGAG610.30007870916961826No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT600.29515938606847697Illumina Single End Adapter 2 (95% over 24bp)
CCGACGGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT590.29024006296733573No Hit
CCGACGGGGGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATT560.27548209366391185No Hit
CCGACGGGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGG540.26564344746162927No Hit
CCGACGGGGCACTGACCTGAACGATACCGAATCTGACTCCACTACGCTGGTG530.260724124360488No Hit
CCGACGGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTA530.260724124360488No Hit
CCGACGGGGGTCCTGGCTATCCAATAGCATCGAGTGGAGCATCCCGGGAAGT520.25580480125934674No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCT520.25580480125934674No Hit
CCGACGAGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC500.24596615505706412No Hit
CCGACGGGAGTAATGGCAGCATTTGTCTTGATATTCTAAGACCACAGTGGTC490.24104683195592286No Hit
CCGACGGGGGTCTGGCATATTTTGGACTCAATTAATGTCCACTGGAGATTGG490.24104683195592286No Hit
CCGACGGGGTGCGGAAGTGCCCTCCTTTCCTTTCTGCTGGGTGCCGGCTGCC470.2312081857536403No Hit
CCGACGGGAGTTGTGTTTGACTCACAACCCCAGAAACAGACATCATGGTGCA470.2312081857536403No Hit
CCGACGGGGGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATCCGCTTCCAT430.21153089334907516No Hit
CCGACGAGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC420.2066115702479339No Hit
CCGACGGGTGGCCCTGAGGCCAGCCTTGCCCAGCTCATTAGCGCCTTGCGCC420.2066115702479339No Hit
CCGACGGGGCGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA420.2066115702479339No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGCAC410.2016922471467926No Hit
CCGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGCG410.2016922471467926No Hit
CCGACGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGACTT390.19185360094451004No Hit
CCGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATC390.19185360094451004No Hit
CCGACGGGATGCGCACCGAGGGCGGCCTGCTCGTTGCAGAGACGGCCAAGGA380.18693427784336875No Hit
CCGACGGGGGCCTGGATGCTGCCGGAAAAAGCCGAGGACGATACCCGCCCCT380.18693427784336875No Hit
CCGACGGGACGCCCACCTACGGGGACCTCAACCACCTCGTGTCAGCCACCAT380.18693427784336875No Hit
CCGACGGGCTCCAGACGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCC370.18201495474222748No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCTC360.1770956316410862No Hit
CCGACGGGGCCTGTCCCGTCGCGACCATGTCGTTCTGCAGCTTCTTCGGAGG360.1770956316410862No Hit
CCGACGGGGGGGAATTCCCTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG360.1770956316410862No Hit
CCGACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC350.17217630853994492No Hit
CCGACGGCGCCGAGGTGCTATCAGTGCTGAAGTTTACACTGAGGAGGATGCT350.17217630853994492No Hit
CCGACGGGGGATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAA350.17217630853994492No Hit
CCGACGGGGAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG330.16233766233766234No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGCCGGGGCCG330.16233766233766234No Hit
CCGACGGGGAGGAGTTAGGCGCGATGCTTTCCACTGCTGCTTACCGAGACGT330.16233766233766234No Hit
CCGACGGGGCTTCCTCGAGGTCACAGCAGTGCTGTCTGAGACATTCGGTGAG320.15741833923652104No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGCCGGGGAAG320.15741833923652104No Hit
CCGACGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA320.15741833923652104Illumina Single End Adapter 2 (100% over 34bp)
CCGACGGGGCACAGCCGGCTTGGAGCGTGTAGGACGGGCACGGAGCAGGGTT320.15741833923652104No Hit
CCGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGAAGCA310.15249901613537978No Hit
CCGACGGGGGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGAAAGGTGAACG310.15249901613537978No Hit
CCGACGGGGAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATCTC310.15249901613537978No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG310.15249901613537978No Hit
CCGACGGGGTTTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAG310.15249901613537978No Hit
CCGACGGGAGTAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTATGT300.14757969303423848No Hit
CCGACGGGCTTTCTCTGCGGAATCACCATGGCGGCTGGGACCCTGTACACAT300.14757969303423848No Hit
CCGACGGGCAGTATGGACACCTATGCGTAACCTCAGCTGGTCATGGGGTCGA300.14757969303423848No Hit
CCGACGGGGTCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGA290.14266036993309722No Hit
CCGACGGGGGAGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG280.13774104683195593No Hit
CCGACGGGATGTGACCCGGTCCTCAGGTCAGTCAACAGGGCCTGCAAGAGAG280.13774104683195593No Hit
CCGACGGGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAATT280.13774104683195593No Hit
CCGACGGGGCATTGAAGAAGAAACCTCGGGGAGGTCTGGAGCTGTCTTTCCT270.13282172373081463No Hit
CCGACGGGAGCTCTGCTCTGACAGCCTTGGGTGCCTGTGCGGTGGGCGGGGC270.13282172373081463No Hit
CCGACGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA270.13282172373081463Illumina Single End Adapter 2 (100% over 34bp)
CCGACGGGGGTCTTCTGACAAATAAAAAGCATTTATTTCACTGCAAAAAAAA260.12790240062967337No Hit
CCGACGGGTCTGATTTCAGTCATGGCCTCCGGCAACGCGCAAATCGGAAAGT260.12790240062967337No Hit
CCGACGGGGGACTGGAGATTTCTCAAAGAACTTAAAACAGAGATATCATTTT260.12790240062967337No Hit
CCGACGGGATTGTCAAGTGTTAACAGCACAAGCGATGGGTTAACTTAGAATA260.12790240062967337No Hit
CCGACGGGTGCTCCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGG260.12790240062967337No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCCGCGGGGCGG260.12790240062967337No Hit
CCGACGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGGCACCAGCAGGC250.12298307752853206No Hit
CCGACGGGGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCTGCT250.12298307752853206No Hit
CCGACGGGGGAAAGGCCAGACTTGTAGGCATTGATAGGGCAGAGTGCTGAGA240.11806375442739078No Hit
CCGACGGGGGGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGA240.11806375442739078No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCTCCGACGGGGCGG240.11806375442739078No Hit
CCGACGGGGAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCAC240.11806375442739078No Hit
CCGACGGGGGACGATACCGATAAGTATCATGCGATACTCAATGGTATCGCTG230.1131444313262495No Hit
CCGACGGGGAAATAAAAAGCATTTATTTCACTGCAAAAAAAAAAAAAAAAAA220.10822510822510822No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT220.10822510822510822No Hit
CCGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTG220.10822510822510822No Hit
CCGACGGGGATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTG220.10822510822510822No Hit
CCGACGGGCTCCAGACGGGATCAGGTCAAAACCAACCCGGTGAGCTCCCTCC220.10822510822510822No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTTGACTCACAACCCCAG220.10822510822510822No Hit
CCGACGGGGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCTGGT220.10822510822510822No Hit
CCGACGGGAGAGATCAACCCGTACCTTCTGGGCACCATGGCTGGGGGTGCAG210.10330578512396695No Hit
CCGACGGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT210.10330578512396695Illumina Single End Adapter 2 (100% over 29bp)
CCGACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT210.10330578512396695Illumina Single End Adapter 2 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAA405.156835E-946.024
GCGGGCA405.156835E-946.09
TAGCGCC405.156835E-946.044
TAGCGCA405.156835E-946.031
TTTTGCT452.7830538E-1046.029
AGGGGAC206.1653665E-446.07
ACAAACA405.156835E-946.020
GGCATTC405.156835E-946.012
CACATTT253.2992226E-546.011
AACAAAC405.156835E-946.019
GTGCAGG206.1653665E-446.031
TCGATTC452.7830538E-1046.020
ACAGACA206.1653665E-446.036
TTGTAGC405.156835E-946.041
AAACAGT405.156835E-946.022
ACGCAGG206.1653665E-446.024
CAGTAAT405.156835E-946.025
TTCGATT405.156835E-946.019
TGTTTTG452.7830538E-1046.027
CTCCACT206.1653665E-446.037
Produced by FastQC (version 0.11.5)