FastQCFastQC Report
Fri 17 Jun 2016
SRR1527382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26160
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC23689.051987767584098No Hit
CCGACGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA18867.209480122324159No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT17126.5443425076452595No Hit
CCGACGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA12454.759174311926605No Hit
CCGACGGGGCGGGCATTCAACAAACAGTAATAGCGCACATGCACTGCCAGCT5742.194189602446483No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG5402.064220183486239No Hit
CCGACGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGAA3341.2767584097859326No Hit
CCGACGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT2801.0703363914373087No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2440.9327217125382262No Hit
CCGACGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC2340.8944954128440368No Hit
CCGACGGGCAGAGGTTTCCAAACTACTTGAAGGTCTTTTAAAAATGTTCCAG2320.8868501529051988No Hit
CCGACGGGGGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAAC2220.8486238532110092No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGGAG2110.8065749235474006No Hit
CCGACGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGC1870.7148318042813456No Hit
CCGACGGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA1840.7033639143730887No Hit
CCGACGGGGATGGAATTATAAATTTCCCCAATTCCTTTCATGCGACAAAGGA1790.6842507645259939No Hit
CCGACGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG1790.6842507645259939No Hit
CCGACGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC1780.6804281345565749No Hit
CCGACGGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGC1660.6345565749235474No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1410.5389908256880734Illumina Single End Adapter 2 (100% over 25bp)
CCGACGGGGTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCGACCCAC1270.48547400611620795No Hit
CCGACGGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA1240.4740061162079511No Hit
CCGACGGGGGATAAAGACATTTGAAAATCTGTCTTCTGACAAATAAAAAGCA1110.4243119266055046No Hit
CCGACGGACTAACACTAAGTGTATACTCTTGTTTTTCATCAACCATTTAAGA1050.40137614678899086No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA980.3746177370030581No Hit
CCGACGGGTGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGG940.35932721712538224No Hit
CCGACGGGGCAGCTCTATTTCGGCGCGCTGGTCGCGGGGCTCCGGGCCGGGC930.35550458715596334No Hit
CCGACGGGGAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGAC910.34785932721712537No Hit
CCGACGGGGGTAAATAATGGATTGTGTTTTATTTGTGCAATATTATTTATTA890.34021406727828746No Hit
CCGACGGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT880.3363914373088685No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTGAA870.33256880733944955No Hit
CCGACGGGGCCTTCCAGGCGACCATTCCGGATGTGCTGCCCAAGGAGGGGGA850.32492354740061163No Hit
CCGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCG840.3211009174311927No Hit
CCGACGGGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGG830.3172782874617737No Hit
CCGACGGGGCACTGACCTGAACGATACCGAATCTGACTCCACTACGCTGGTG820.3134556574923547No Hit
CCGACGGGGGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG800.3058103975535168No Hit
CCGACGGGGGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATT780.2981651376146789No Hit
CCGACGGGATCTTCCCTGGCCCCTGGGTCTCTTTCCTGTGGGAGTGTAACTG720.27522935779816515No Hit
CCGACGGGGTGCGGAAGTGCCCTCCTTTCCTTTCTGCTGGGTGCCGGCTGCC700.2675840978593272No Hit
CCGACGGGATGCGCACCGAGGGCGGCCTGCTCGTTGCAGAGACGGCCAAGGA680.2599388379204893No Hit
CCGACGAGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC650.2484709480122324No Hit
CCGACGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCTC620.23700305810397554No Hit
CCGACGGGTGGCCCTGAGGCCAGCCTTGCCCAGCTCATTAGCGCCTTGCGCC620.23700305810397554No Hit
CCGACGGGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCT590.22553516819571864No Hit
CCGACGGGGGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATCCGCTTCCAT590.22553516819571864No Hit
CCGACGGGAGTTGTGTTTGACTCACAACCCCAGAAACAGACATCATGGTGCA580.2217125382262997No Hit
CCGACGGGCTCCAGACGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCC570.21788990825688076No Hit
CCGACGGGGAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG560.21406727828746178No Hit
CCGACGGGGGTCTGGCATATTTTGGACTCAATTAATGTCCACTGGAGATTGG560.21406727828746178No Hit
CCGACGGGGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCTGCT560.21406727828746178No Hit
CCGACGGGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTA560.21406727828746178No Hit
CCGACGGGGCTTCCTCGAGGTCACAGCAGTGCTGTCTGAGACATTCGGTGAG550.2102446483180428No Hit
CCGACGGGAGCTCTGCTCTGACAGCCTTGGGTGCCTGTGCGGTGGGCGGGGC550.2102446483180428No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT540.20642201834862386Illumina Single End Adapter 2 (95% over 24bp)
CCGACGGGGAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATCTC530.20259938837920488No Hit
CCGACGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC530.20259938837920488No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGCAC520.19877675840978593No Hit
CCGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGCG520.19877675840978593No Hit
CCGACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC520.19877675840978593No Hit
CCGACGGGGGTCCTGGCTATCCAATAGCATCGAGTGGAGCATCCCGGGAAGT520.19877675840978593No Hit
CCGACGGGGGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCC510.19495412844036697No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGGCGGGGAAG500.191131498470948No Hit
CCGACGGGGGGGAATTCCCTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG480.1834862385321101No Hit
CCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCG460.17584097859327216No Hit
CCGACGGGGGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGAAAGGTGAACG460.17584097859327216No Hit
CCGACGGGAGTAATGGCAGCATTTGTCTTGATATTCTAAGACCACAGTGGTC460.17584097859327216No Hit
CCGACGAGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC460.17584097859327216No Hit
CCGACGGGGTCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGA440.16819571865443425No Hit
CCGACGGGGGATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAA440.16819571865443425No Hit
CCGACGGGGTTTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAG440.16819571865443425No Hit
CCGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGAAGCA430.1643730886850153No Hit
CCGACGGGGGCCTGGATGCTGCCGGAAAAAGCCGAGGACGATACCCGCCCCT430.1643730886850153No Hit
CCGACGGGGGACTGGAGATTTCTCAAAGAACTTAAAACAGAGATATCATTTT430.1643730886850153No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGCAG420.16055045871559634No Hit
CCGACGGGGATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTG410.15672782874617736No Hit
CCGACGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGACTT400.1529051987767584No Hit
CCGACGGGGCATTGAAGAAGAAACCTCGGGGAGGTCTGGAGCTGTCTTTCCT400.1529051987767584No Hit
CCGACGGGAGTAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTATGT390.14908256880733944No Hit
CCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAG380.1452599388379205No Hit
CCGACGGGACGCCCACCTACGGGGACCTCAACCACCTCGTGTCAGCCACCAT380.1452599388379205No Hit
CCGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATC380.1452599388379205No Hit
CCGACGGGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAATT380.1452599388379205No Hit
CCGACGGGGCACAGCCGGCTTGGAGCGTGTAGGACGGGCACGGAGCAGGGTT380.1452599388379205No Hit
CCGACGGGGCGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA380.1452599388379205No Hit
CCGACGGGGGAAAGGCCAGACTTGTAGGCATTGATAGGGCAGAGTGCTGAGA370.14143730886850153No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG370.14143730886850153Illumina Single End Adapter 2 (100% over 23bp)
CCGACGGGGAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCAC370.14143730886850153No Hit
CCGACGGCGCCGAGGTGCTATCAGTGCTGAAGTTTACACTGAGGAGGATGCT370.14143730886850153No Hit
CCGACGGGAGAGATCAACCCGTACCTTCTGGGCACCATGGCTGGGGGTGCAG360.13761467889908258No Hit
CCGACGGGCAGTATGGACACCTATGCGTAACCTCAGCTGGTCATGGGGTCGA350.1337920489296636No Hit
CCGACGGGGGAGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG330.1261467889908257No Hit
CCGACGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA330.1261467889908257Illumina Single End Adapter 2 (100% over 34bp)
CCGACGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAAT320.12232415902140673No Hit
CCGACGGGGAAATAAAAAGCATTTATTTCACTGCAAAAAAAAAAAAAAAAAA310.11850152905198777No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGGCGGGGGAG290.11085626911314984No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGTAG280.10703363914373089No Hit
CCGACGGGATGTGACCCGGTCCTCAGGTCAGTCAACAGGGCCTGCAAGAGAG280.10703363914373089No Hit
CCGACGGGGGACGATACCGATAAGTATCATGCGATACTCAATGGTATCGCTG280.10703363914373089No Hit
CCGACGGGGGGACTGAGGCGCATCAAGATGGCGGCCGAGACGGTGGAGCTCC280.10703363914373089No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT270.10321100917431193No Hit
CCGACGGGCTCCAGACGGGATCAGGTCAAAACCAACCCGGTGAGCTCCCTCC270.10321100917431193No Hit
CCGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATA270.10321100917431193No Hit
CCGACGGGGAGCACTCACCGTCTTTGGTTCGTCCTCAGTGCAGGGCAACAGG270.10321100917431193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCAT253.3258122E-546.035
CTTTTAA206.1984314E-446.035
AGCCTCA253.3258122E-546.025
ACAGTAA405.2586984E-946.024
TCCTTTC253.3258122E-546.033
TGTGACA253.3258122E-546.043
TAGCGCC452.8558134E-1046.044
TAGCGCA405.2586984E-946.031
ACTGTGA206.1984314E-446.041
TTTTGCT452.8558134E-1046.029
ACAAAGG206.1984314E-446.045
ACAAACA405.2586984E-946.020
CCAATTC253.3258122E-546.028
TTCATGC253.3258122E-546.037
AAACTAC206.1984314E-446.020
GGCATTC405.2586984E-946.012
TAAATTT253.3258122E-546.019
AACAAAC405.2586984E-946.019
GCCTCAG253.3258122E-546.026
GTCACCA253.3258122E-546.040
Produced by FastQC (version 0.11.5)