FastQCFastQC Report
Fri 17 Jun 2016
SRR1527381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714557
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49880.6980548787570481No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC45890.6422160863304117No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC45270.6335393817428141No Hit
GATCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG20540.28745082617621825No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGA20530.28731087932803123No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC19860.2779344404994983No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC16110.22545437242935135No Hit
GATCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT15020.21020016597696195No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT14030.19634542800644317No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA13540.1894880324452773No Hit
GATCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC11610.16247829074517497No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11070.15492116094307382No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG10620.14862355277465616No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG10590.148203712230095No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT10590.148203712230095No Hit
GATCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC9680.1354685490450727No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG9300.13015056881396445No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8060.1127971596387692No Hit
GATCTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC7550.1056598703812292No Hit
GATCTTGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCA7340.10272098656930098No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG7330.10258103972111393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAATA302.526367E-644.00000439
TACCACG650.044.00000422
CGAAAGT551.8189894E-1244.00000415
ATAGCGG302.526367E-644.00000439
TGGCATA302.526367E-644.00000436
ATATAGC302.526367E-644.00000437
CGCTAAA302.526367E-644.00000426
CGTACTA302.526367E-644.00000429
TAAGCCG302.526367E-644.00000417
TCGGTAA302.526367E-644.00000420
ACGACTA551.8189894E-1244.00000412
GATACGA207.854481E-444.09
TACCCTT351.4450598E-744.040
ATAGCCG207.854481E-444.025
GAATCGT254.441143E-544.011
GCGTTAG207.854481E-444.026
TGCATAC207.854481E-444.026
TACGCTA207.854481E-444.038
TAACCAC254.441143E-544.033
TACGATC207.854481E-444.011
Produced by FastQC (version 0.11.5)