FastQCFastQC Report
Fri 17 Jun 2016
SRR1527380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731034
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG54290.7426467168421715No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC48110.6581089251662713No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT46270.6329390972239322No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC20710.28329735689448093No Hit
GATCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT20650.28247660163549165No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17070.2335048711824621No Hit
GATCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG16330.22338222298826046No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15680.2144907076825428No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT15160.20737749543796868No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAG14380.1967076770711075No Hit
GATCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC13510.18480672581576232No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG12520.1712642640424385No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG11020.15074538256770548No Hit
GATCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG10660.14582085101376954No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC10580.14472651066845044No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10470.1432217926936367No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC9270.12680668751385024No Hit
GATCTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT8240.11271705556786689No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT7690.1051934656937981No Hit
GATCTTGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC7680.1050566731506332No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7670.10491988060746832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGC351.0188887E-746.00000446
TATTACG351.0188887E-746.00000439
AACTATC351.0188887E-746.00000444
AGACTAC650.046.038
GATACGG206.3099386E-446.037
CGTTGAC206.3099386E-446.039
CCAATTG405.6061253E-946.034
CCAATTA253.4159268E-546.013
TTCGTTA253.4159268E-546.041
TGTATAC253.4159268E-546.025
TACGGTG206.3099386E-446.039
CGATTCA253.4159268E-546.012
ACGATTC253.4159268E-546.011
TACGCTA206.3099386E-446.038
TCTACGG206.3099386E-446.025
CGGATAA206.3099386E-446.045
TGCGACA206.3099386E-446.013
CGCTAAA253.4159268E-546.026
TCGCTAC301.860466E-646.022
CGTACTA301.860466E-646.029
Produced by FastQC (version 0.11.5)