FastQCFastQC Report
Fri 17 Jun 2016
SRR1527379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642342
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG49730.774198168576864No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42470.6611742654224696No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41330.643426710381697No Hit
GATCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT18560.28894265048836915No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC17170.2673030877632165No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAG16890.26294403915671094No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15320.2385022308988047No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT13780.21452746356302407No Hit
GATCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG13660.21265929987452167No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG12990.20222871928038336No Hit
GATCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC11200.17436194426022275No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG10970.17078129719059318No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC9810.15272238153507012No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG9660.15038717692444212No Hit
GATCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG9220.14353724339993337No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8980.1398009160229286No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC8820.13731003110492543No Hit
GATCTTGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC7450.11598182899452317No Hit
GATCTTGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC7340.11426934561339598No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT7340.11426934561339598No Hit
GATCTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT7150.1113114197732672No Hit
GATCTTGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT6860.10679669085938642No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.10539556809300965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGTA551.8189894E-1246.00000412
TACCCGA253.4154604E-546.040
AAATCGT206.309365E-446.018
TACTTGC206.309365E-446.016
CATGCGA206.309365E-446.019
ATTACGC206.309365E-446.040
ATAGCGG501.6370905E-1146.039
CCAATCG253.4154604E-546.020
TAACGAG253.4154604E-546.016
TTATACG301.8601113E-646.032
TCGTACA206.309365E-446.019
ACGTCGG253.4154604E-546.043
CGTAACG206.309365E-446.032
TATACGC301.8601113E-646.033
TCGGTAC206.309365E-446.045
ACGACTA206.309365E-446.012
AATCGTA253.4154604E-546.014
CGTACCG351.0186341E-745.99999617
TGGGATA8000.043.9875036
AGACTAC900.043.44444738
Produced by FastQC (version 0.11.5)