##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642342
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.620874861055327	31.0	31.0	33.0	28.0	33.0
2	28.77669839431331	31.0	28.0	31.0	25.0	33.0
3	30.031349343496142	31.0	30.0	33.0	25.0	33.0
4	34.80113397535892	35.0	35.0	37.0	33.0	37.0
5	35.36558717941533	35.0	35.0	37.0	33.0	37.0
6	36.041129180405456	37.0	35.0	37.0	35.0	37.0
7	36.138820130086465	37.0	35.0	37.0	35.0	37.0
8	36.34100370207771	37.0	37.0	37.0	35.0	37.0
9	38.411590710244695	39.0	39.0	39.0	37.0	39.0
10	36.91530679918174	39.0	37.0	39.0	33.0	39.0
11	36.902178278860795	39.0	37.0	39.0	33.0	39.0
12	36.857183556423216	39.0	37.0	39.0	33.0	39.0
13	36.84150966307668	39.0	37.0	39.0	33.0	39.0
14	37.696583751334956	40.0	37.0	40.0	33.0	40.0
15	37.769484168869546	40.0	37.0	40.0	33.0	40.0
16	37.813986941535816	40.0	37.0	40.0	33.0	40.0
17	37.8269239750787	40.0	37.0	40.0	33.0	40.0
18	37.738606536704744	40.0	37.0	40.0	33.0	40.0
19	37.65214792120086	40.0	37.0	40.0	33.0	40.0
20	37.55066304242911	40.0	37.0	40.0	33.0	40.0
21	37.61702488705394	40.0	37.0	40.0	33.0	40.0
22	37.62325054254587	40.0	37.0	40.0	33.0	40.0
23	37.50058224434958	40.0	37.0	40.0	33.0	40.0
24	37.470003518374945	39.0	37.0	40.0	33.0	40.0
25	37.3927533930523	39.0	37.0	40.0	33.0	40.0
26	37.2256912984049	39.0	36.0	40.0	33.0	40.0
27	37.11213185499314	39.0	36.0	40.0	32.0	40.0
28	36.903710173085365	39.0	36.0	40.0	31.0	40.0
29	36.83969754429883	39.0	35.0	40.0	31.0	40.0
30	36.698092293513426	39.0	35.0	40.0	31.0	40.0
31	36.59049229226798	39.0	35.0	40.0	31.0	40.0
32	36.472203281118155	39.0	35.0	40.0	31.0	40.0
33	36.32835156349733	38.0	35.0	40.0	30.0	40.0
34	36.008149864091095	38.0	35.0	40.0	30.0	40.0
35	35.88287859115549	38.0	35.0	40.0	30.0	40.0
36	35.740024784304936	38.0	35.0	40.0	29.0	40.0
37	35.58273629935455	38.0	34.0	40.0	29.0	40.0
38	35.48229136503607	38.0	34.0	40.0	29.0	40.0
39	35.271792907827916	38.0	34.0	40.0	28.0	40.0
40	35.401233299395024	38.0	34.0	40.0	28.0	40.0
41	35.506289484417955	38.0	35.0	40.0	29.0	40.0
42	35.47466458677776	38.0	35.0	40.0	29.0	40.0
43	35.43094177245143	38.0	35.0	40.0	29.0	40.0
44	35.3047410880808	38.0	34.0	40.0	29.0	40.0
45	35.20459505995249	37.0	34.0	40.0	28.0	40.0
46	34.86716266412596	37.0	34.0	40.0	27.0	40.0
47	34.83161462273991	37.0	34.0	40.0	27.0	40.0
48	34.68405304339433	36.0	34.0	40.0	27.0	40.0
49	34.49040230905032	36.0	33.0	40.0	27.0	40.0
50	34.26159896130099	36.0	33.0	39.0	26.0	40.0
51	34.16057022582985	35.0	33.0	39.0	26.0	40.0
52	33.60815889354892	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	14.0
14	14.0
15	33.0
16	82.0
17	139.0
18	253.0
19	432.0
20	725.0
21	1121.0
22	1623.0
23	2371.0
24	3230.0
25	4464.0
26	6010.0
27	7957.0
28	10014.0
29	11823.0
30	13213.0
31	15753.0
32	20143.0
33	28550.0
34	45807.0
35	50377.0
36	82676.0
37	125677.0
38	192389.0
39	17441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.67338271512683	0.15817119229320206	0.13388506434267103	0.03456102823729415
8	99.318275934004	0.4194027480687858	0.20580936635001293	0.05651195157719719
9	97.31918510699907	1.5745506287927615	0.46517275843709427	0.641091505771069
10	56.059077563042734	29.890930376652936	4.8902298152697465	9.159762245034576
11	32.0385402168938	27.902114449934768	20.952545528705894	19.106799804465535
12	28.899246818672918	19.643429823987844	26.684850126568087	24.772473230771148
13	22.57520137247759	19.182616114157256	29.40738734194557	28.834795171419586
14	24.546114063847607	21.370391473700927	28.044873291797828	26.03862117065364
15	26.241783971778275	19.55873973677574	27.605076423462894	26.5943998679831
16	28.90220474451305	20.105021935355307	25.25554922455639	25.737224095575257
17	30.52423786705524	20.390539619081423	22.650239280632437	26.434983233230895
18	29.447864221863117	21.463176936896545	21.486373302695448	27.602585538544886
19	29.15020347416174	20.09241183045792	23.67695090777187	27.08043378760847
20	28.250838338455214	22.76232910194258	22.326268560984648	26.660563998617558
21	30.581528220169318	21.64454449498865	24.182756226433895	23.59117105840814
22	30.40623219406484	20.607869328177202	22.533634730408412	26.45226374734954
23	29.35103107067575	22.770424477926092	22.690093439320485	25.188451012077678
24	29.636081713479733	21.70977454377886	22.448788962888926	26.20535477985248
25	27.548564471885694	20.494534064408057	22.75283260319269	29.20406886051356
26	29.002151501847923	20.309585859246322	22.025805567750513	28.662457071155238
27	28.230911259111192	19.67378748392601	22.3096107680955	29.7856904888673
28	31.280999841206086	21.457105404908912	21.43966921048289	25.822225543402112
29	31.10025500434348	20.80464923669945	22.025961248057886	26.06913451089918
30	29.532554309075227	21.605001696915348	20.689445809241803	28.172998184767618
31	32.29587976498501	23.079605568373236	20.303981368180814	24.320533298460944
32	33.17485078042538	22.496582817253113	20.860382786739773	23.46818361558173
33	32.776776234467	21.642832011607524	21.34953031251265	24.23086144141283
34	29.536601997066985	24.861678046897136	22.507636119076754	23.09408383695913
35	30.774571801314565	24.46625006616413	22.67390268735347	22.08527544516784
36	30.749974312749284	23.678196350230873	20.698319586762192	24.87350975025765
37	29.544230332128368	25.64225910807638	20.5695719725629	24.243938587232346
38	32.291987757300625	23.873108095064623	20.74611344112638	23.088790706508373
39	31.046856658913786	23.585410887035255	19.79926581167042	25.56846664238054
40	29.04901127436786	24.666921982370763	21.15788785413378	25.126178889127598
41	28.555037659066357	23.672903219780117	22.31926294715276	25.452796174000763
42	27.225994875004282	21.732036827733513	23.413228467078284	27.628739830183918
43	26.708513533289118	20.99239968739394	26.370842946592315	25.928243832724622
44	27.362526504572333	20.019397766298948	25.00132328261269	27.61675244651603
45	27.975439874708492	20.578290069775914	24.839104402327735	26.607165653187863
46	27.836417360222438	21.63162302947651	23.95624137920298	26.57571823109808
47	28.12816225624356	21.379109570913936	23.513486585027913	26.979241587814588
48	30.428650158326874	22.325801520062523	22.79471060587662	24.450837715733986
49	27.72541730106392	21.60858234398498	23.9705639674815	26.695436387469602
50	26.022897459608746	22.911937877330146	23.97694686008388	27.088217802977233
51	26.36913046321119	22.795177646798745	23.725211803058183	27.110480086931883
52	27.46200622098508	22.015374987156374	23.818152946561177	26.704465845297364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	11.0
6	19.0
7	53.5
8	88.0
9	471.5
10	855.0
11	785.5
12	716.0
13	774.5
14	806.5
15	780.0
16	835.0
17	890.0
18	1077.5
19	1265.0
20	1339.5
21	1414.0
22	1802.5
23	2191.0
24	2635.5
25	3080.0
26	3585.0
27	4090.0
28	5355.0
29	6620.0
30	7115.0
31	7610.0
32	7877.0
33	8144.0
34	8693.0
35	9242.0
36	9634.0
37	10026.0
38	10676.0
39	11872.5
40	12419.0
41	13154.5
42	13890.0
43	15296.0
44	16702.0
45	17738.5
46	18775.0
47	20172.5
48	21570.0
49	24014.0
50	26458.0
51	29573.5
52	32689.0
53	36170.0
54	39651.0
55	45743.5
56	51836.0
57	58867.5
58	65899.0
59	60629.0
60	55359.0
61	54127.5
62	52896.0
63	47707.5
64	40736.5
65	38954.0
66	31197.5
67	23441.0
68	20831.0
69	18221.0
70	16021.5
71	13822.0
72	15318.0
73	16814.0
74	12041.5
75	7269.0
76	5002.5
77	2736.0
78	1837.5
79	939.0
80	560.0
81	181.0
82	113.5
83	46.0
84	42.5
85	39.0
86	29.5
87	20.0
88	11.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	642342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.529110660676087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74135338345866	11.451376369597504
2	7.762406015037595	2.41086524001233
3	2.6977443609022553	1.2568071214399805
4	1.3243107769423559	0.8226147441705509
5	0.8340852130325813	0.6476300786808273
6	0.6335839598997494	0.5903397255667542
7	0.5343358395989974	0.5808432268168671
8	0.44010025062656644	0.5467492395016984
9	0.4551378446115288	0.6361097359350626
>10	9.230075187969925	35.994065466682855
>50	1.5398496240601502	16.126456000074725
>100	0.7639097744360902	21.69981100410685
>500	0.031077694235588975	3.1252821705571177
>1k	0.012030075187969924	4.111049876856876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4973	0.774198168576864	No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4247	0.6611742654224696	No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4133	0.643426710381697	No Hit
GATCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1856	0.28894265048836915	No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1717	0.2673030877632165	No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAG	1689	0.26294403915671094	No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1532	0.2385022308988047	No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1378	0.21452746356302407	No Hit
GATCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1366	0.21265929987452167	No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1299	0.20222871928038336	No Hit
GATCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1120	0.17436194426022275	No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1097	0.17078129719059318	No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	981	0.15272238153507012	No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	966	0.15038717692444212	No Hit
GATCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	922	0.14353724339993337	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	898	0.1398009160229286	No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	882	0.13731003110492543	No Hit
GATCTTGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC	745	0.11598182899452317	No Hit
GATCTTGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC	734	0.11426934561339598	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	734	0.11426934561339598	No Hit
GATCTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	715	0.1113114197732672	No Hit
GATCTTGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT	686	0.10679669085938642	No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.10539556809300965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.227212295007956E-4	0.0	0.0	0.0	0.0
8	9.340818442511933E-4	0.0	0.0	0.0	0.0
9	0.0020238439958775853	0.0	0.0	0.0	0.0
10	0.028956537171786992	0.0	0.0	0.0	0.0
11	0.06118236079845316	0.0	0.0	0.0	0.0
12	0.08795937366698736	0.0	0.0	0.0	0.0
13	0.10399444532663285	0.0	0.0	0.0	0.0
14	0.1254783277444103	0.0	0.0	0.0	0.0
15	0.14493836616631015	0.0	0.0	0.0	0.0
16	0.16393136366608443	0.0	0.0	0.0	0.0
17	0.17809827163722752	0.0	0.0	0.0	0.0
18	0.193354941759997	0.0	0.0	0.0	0.0
19	0.2097013740343929	0.0	0.0	0.0	0.0
20	0.22791596999729116	0.0	0.0	0.0	0.0
21	0.24535216442331342	0.0	0.0	0.0	0.0
22	0.2665246862263405	0.0	0.0	0.0	0.0
23	0.28831992925886835	0.0	0.0	0.0	0.0
24	0.31338445874627535	0.0	0.0	0.0	0.0
25	0.3347126608566776	0.0	0.0	0.0	0.0
26	0.3575976660408318	0.0	0.0	0.0	0.0
27	0.3804826712249861	0.0	0.0	0.0	0.0
28	0.422672034523665	0.0	0.0	0.0	0.0
29	0.443844556326692	0.0	0.0	0.0	0.0
30	0.46937612673622464	0.0	0.0	0.0	0.0
31	0.4847884771663693	0.0	0.0	0.0	0.0
32	0.507206441428398	0.0	0.0	0.0	0.0
33	0.5215290297069163	0.0	0.0	0.0	0.0
34	0.5402106665919402	0.0	0.0	0.0	0.0
35	0.5777296206693631	0.0	0.0	0.0	0.0
36	0.5993691833945157	0.0	0.0	0.0	0.0
37	0.6163383368984123	0.0	0.0	0.0	0.0
38	0.6309722857916811	0.0	0.0	0.0	0.0
39	0.6471630377587018	0.0	0.0	0.0	0.0
40	0.6658446746437257	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGTA	55	1.8189894E-12	46.000004	12
TACCCGA	25	3.4154604E-5	46.0	40
AAATCGT	20	6.309365E-4	46.0	18
TACTTGC	20	6.309365E-4	46.0	16
CATGCGA	20	6.309365E-4	46.0	19
ATTACGC	20	6.309365E-4	46.0	40
ATAGCGG	50	1.6370905E-11	46.0	39
CCAATCG	25	3.4154604E-5	46.0	20
TAACGAG	25	3.4154604E-5	46.0	16
TTATACG	30	1.8601113E-6	46.0	32
TCGTACA	20	6.309365E-4	46.0	19
ACGTCGG	25	3.4154604E-5	46.0	43
CGTAACG	20	6.309365E-4	46.0	32
TATACGC	30	1.8601113E-6	46.0	33
TCGGTAC	20	6.309365E-4	46.0	45
ACGACTA	20	6.309365E-4	46.0	12
AATCGTA	25	3.4154604E-5	46.0	14
CGTACCG	35	1.0186341E-7	45.999996	17
TGGGATA	800	0.0	43.987503	6
AGACTAC	90	0.0	43.444447	38
>>END_MODULE