FastQCFastQC Report
Fri 17 Jun 2016
SRR1527378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621755
Sequences flagged as poor quality0
Sequence length50
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA961175.926727526660932No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA490743.0259811130534513No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA382382.357816069628273No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA225591.3910239216157805No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAA206101.270845472959849No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA124210.7658986715009357No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107180.6608889752151219No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAA88780.5474316404142426No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGA75850.4677031980786247No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAA65420.40339015449312626No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59830.3689213228878591No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53590.33044448760756095Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGG50460.31114440837241136No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA46570.2871580479172255No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA46190.2848149073072073No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAA45610.2812385347971796No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAA43870.2705094172670965No Hit
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA43690.26939950855708783No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAA41230.2542307561869703No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAA40380.24898952061192967No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAA38540.23764378713184175No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAA33410.20601138889659662No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAA30930.19071931333647807No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA29960.1847381386214317Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA29730.18331992193642074Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCT29680.18301161396141835Illumina Single End Adapter 2 (96% over 27bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCG28450.17542723777635957Illumina Single End Adapter 2 (95% over 23bp)
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA27020.16660962969129123No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA26430.16297159558626303No Hit
TAGTCGGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAA25190.15532555780620377No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA24540.1513175541311727No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGCAAAAAAAAAAAAAAAAAAAAA23310.14373317794611393No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCAAAAAAAAAAAAAAAAAAAAAAAA23300.14367151635111347No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAAAAAAAAAAAAAAAAAAAAA23180.14293157721110772No Hit
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA21890.13497723145604607Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGTGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA21020.12961267269100452No Hit
TAGTCGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA20820.12837944079099492No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCCAAAAAAAAAAAAAAAAAAAAAAA19830.12227494288594763No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA19640.12110337258093855Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA18660.11506053627089172Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA18560.11444392032088693No Hit
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAA17940.11062090143085732No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAA17410.10735283689583199No Hit
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAA17260.10642791297082482No Hit
TAGTCGGGGTGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAA16450.1014333237757861No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAA16420.10124833899078467No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGT16300.10050839985077895Illumina Single End Adapter 2 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATTA302.5283625E-644.00000422
CATGAAC650.044.00000418
ACATGAA650.044.00000417
GATTGAT600.044.0000049
ATTGATC600.044.00000410
GGTTAGG302.5283625E-644.00000410
TGTCGCT650.044.00000429
TCGCGGC302.5283625E-644.00000444
TAACTAC302.5283625E-644.00000442
CGGGTTT254.443652E-544.05
CGGGTTC207.8574405E-444.05
ATCCTAG207.8574405E-444.044
AATCCTA207.8574405E-444.043
GTCGCCC207.8574405E-444.030
ATCTATC254.443652E-544.032
CGACGTG207.8574405E-444.014
CTATCTT254.443652E-544.034
CCCACGT254.443652E-544.012
ATATCAT207.8574405E-444.023
ACCTAGC207.8574405E-444.029
Produced by FastQC (version 0.11.5)