FastQCFastQC Report
Fri 17 Jun 2016
SRR1527377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294465
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA779366.020711259091594No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA389073.0056432580255166No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA307342.374262726300054No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA179101.3835831791512325No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA160891.2429073014720369No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA99790.7708976295226213No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84330.6514660496807562No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA70990.5484118921716694No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA61840.4777263193674607Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA54470.420791601163415No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47850.36965078236955035No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA39560.3056088808890159No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG37430.2891542065641018No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA35210.2720042643099659No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA34120.26358379716716945Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA33820.261266237403097No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA33240.2567856218592237No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA32600.2518414943625359No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA32300.24952393459846345No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA31530.24357553120401093No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA30760.2376271278095584No Hit
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30320.2342280401555855Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC26790.20695808693166673Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA25250.19506128014276167No Hit
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA25180.19452051619781144Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC25070.1936707442843182Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA22670.17513026617173888No Hit
TAGTCGGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA21210.1638514753199198No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA20880.16130215957944016No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA19910.15380871634227267No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGCAAAAAAAAAAAAAAAAAAAAAAA19460.15033237669616403No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA19120.14770580896354862No Hit
TAGTCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA18480.14276168146686083Illumina Single End Adapter 2 (96% over 32bp)
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA18450.1425299254904536Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAAAAAAAAAAAAAAAAAAAAAAA17660.13642701811172955No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA16550.12785204698466163Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGTGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA16490.12738853503184713No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA16300.12592074718126794No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAG16230.1253799832363177No Hit
TAGTCGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA15970.12337143144078828No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCAAAAAAAAAAAAAAAAAAAAAAAAAA15810.12213539956661633No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAA14710.11363768043168414No Hit
TAGTCGGGGGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA14700.11356042843954839Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAA14520.11216989258110494No Hit
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAA14500.11201538859683344No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGTCT14470.1117836326204262Illumina Single End Adapter 2 (96% over 26bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCCAAAAAAAAAAAAAAAAAAAAAAAAA14050.10853904895072482No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAA13500.10429018938325874No Hit
TAGTCGGGGTGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA13340.10305415750908675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTC700.046.00000422
CCTAGCT351.0197073E-746.00000430
CTGAGAA351.0197073E-746.00000423
AAGACTA351.0197073E-746.00000445
CCTACGA700.046.00000415
TAGCCCG351.0197073E-746.00000441
TAGCATC351.0197073E-746.00000442
ACCTAGC351.0197073E-746.00000429
CTACGAG700.046.00000416
CGAGGCA351.0197073E-746.0000045
GAACCTA351.0197073E-746.00000427
ATTATAG351.0197073E-746.00000438
ACGAGCA700.046.00000418
TGAGAAC351.0197073E-746.00000424
TGATCAC600.046.012
TGATATT206.311752E-446.016
ACAGTCC206.311752E-446.039
CTCGTCG501.6370905E-1146.040
CAGCCGG253.4173958E-546.046
ACACCTT301.8615865E-646.029
Produced by FastQC (version 0.11.5)