FastQCFastQC Report
Fri 17 Jun 2016
SRR1527376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606155
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA936945.833434506632299No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA470142.9271147554252237No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA363542.2634179142112685No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA212871.3253390862027636No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA195921.2198075528202446No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA115220.7173653850344456No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109610.6824372492069569No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA85050.5295254816627287No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG73120.45524871509910314No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA66240.41241349682938444No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61850.3850811409857704No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA50260.3129212311389623No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA41780.2601243342018672No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA41680.2595017292851562No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA41370.257571654043352Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA41000.25526801585152115No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA39530.24611572357586906No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA39170.24387434587570936No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA39100.24343852243401165No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA38750.24125940522552305No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA30730.19132649090529869No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA28970.1803686443711846No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC28950.1802441233878424Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC27950.17401807422073212Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA25850.16094337096980055No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA25140.15652287606115226No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25040.15590027114444124Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA25010.1557134896694279Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA23360.14544050854369597No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA22740.1415803580600876No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGCAAAAAAAAAAAAAAAAAAAAAAA22530.14027288773499444No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAAAAAAAAAAAAAAAAAAAAAAA22210.13828055200151915No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCAAAAAAAAAAAAAAAAAAAAAAAAAA20000.12452098334220546No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAG19790.1232135130171123No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA19630.12221734515037465No Hit
TAGTCGGGTGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA18720.11655164040830429No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCCAAAAAAAAAAAAAAAAAAAAAAAAA18670.11624033794994879No Hit
TAGTCGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA18670.11624033794994879No Hit
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAA18360.11431026270814461No Hit
TAGTCGGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAA17070.10627865928257237No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGTCT17010.10590509633254573Illumina Single End Adapter 2 (96% over 26bp)
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16640.10360145814071495Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16550.10304111371567501Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAA16470.10254302978230619No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAAA16380.10198268535726626No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAA16170.10067521503217311No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC206.312208E-446.039
TATTTCG253.4177672E-546.043
ATCTATC206.312208E-446.032
CTATCTT206.312208E-446.034
TCACATG405.6134013E-946.015
TCACATA253.4177672E-546.031
TTTCGTA253.4177672E-546.045
ATAGCAT253.4177672E-546.041
GTTAGGC453.110472E-1046.039
CCTAACT206.312208E-446.040
TCCTAGA253.4177672E-546.045
GACCCAC301.8618684E-646.011
CTAACTA206.312208E-446.041
GCACATT206.312208E-446.021
ACGGCGT253.4177672E-546.024
GTATGTA253.4177672E-546.016
CACTAAC206.312208E-446.09
TCGCTAC253.4177672E-546.036
CCATAAT206.312208E-446.021
CCATAAC206.312208E-446.043
Produced by FastQC (version 0.11.5)