FastQCFastQC Report
Fri 17 Jun 2016
SRR1527372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19226506
Sequences flagged as poor quality0
Sequence length50
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA2379901.2378224103745112No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1353620.7040384768818629No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1162140.6044467986018884Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAA1122100.5836213818569012No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1049930.5460846604161984Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA814070.4234102649748217No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA795870.413944166454373No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA748900.3895143506573685No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAG724890.37702638222462265No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA690680.35923323769799875Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA674530.3508333755493588No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA646510.3362597447502942Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA567540.2951862392470062Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA549680.28589697992968666No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA477900.24856310345727925Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA477630.24842267232538248No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA468140.24348677809686273No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGAGA436760.22716555987863837No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416690.2167268457409786No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA395750.20583563128942928No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA378960.19710289534666361Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAA364120.1893843842453746No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAA359900.18718949766535845No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAA358370.18639372125127676No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG353180.18369432282703888No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA329290.17126876823069154No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAA301260.15668993627859373No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAA288390.14999605232484778No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA276790.14396271480632No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAA273610.14230874814175803No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAA271070.1409876552713218No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAA265830.13826225108191784No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA265030.13784615883926074No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAA260600.13554204804554712No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA254760.13250457467415036No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA254740.13249417236808392Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAA251260.1306841711125256No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAA246060.1279795715352545No Hit
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA244920.12738664008946815Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA231180.12024025582183263No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA228610.11890355949229672No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAA225020.11703634555337303No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA216770.11274539430097179No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAA216190.1124437274250454No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA214660.11164795101096371No Hit
AATAAAGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA213500.11104461725911094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG10350.042.08695643
GAAGCAG1046500.040.4451039
GGAAGCA1122600.040.280428
AAGCAGT1016100.039.9360310
GGGAAGC1181500.039.6547
ATACACG7300.039.47945442
AGGGAAG1352700.039.438016
TATATGG9750.039.035943
ATAGCGA7150.037.2307744
ATATAGC7350.037.11564642
TATAGCG8650.036.87861343
AGGGACG540150.036.8642046
ATATATG11050.036.83257742
TGATGAA25500.036.7529440
TCATATA13500.036.66666440
GCGCTAG11350.036.6343636
ATAAATT17700.036.542372
GAACAGT74850.036.4462289
ATGAACT15850.036.0883342
AAGGGAC720950.036.0233045
Produced by FastQC (version 0.11.5)