FastQCFastQC Report
Fri 17 Jun 2016
SRR1527371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335282
Sequences flagged as poor quality0
Sequence length52
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG1643330.9479684264726701No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1455690.8397267491812364Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1369160.7898112069939215Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1322870.7631084397704058No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1019130.5878935225859031Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA914310.5274272434679748No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA860090.49614999052221936Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA721030.41593208578897073Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA706630.407625327352621No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA671650.38744682665098845No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA661580.3816378643277911No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA570830.32928798043204605Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA555960.32071009863006555No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA546500.31525301982396364No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA529600.30550411582574777Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG529120.30522722387786944No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA456620.263405002583748No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400590.23108363625120146No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA397530.22931845008347715No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366140.21121087040868444No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA316500.18257562813226808No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA309270.17840494316735084Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA294900.1701154904777436No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA294870.1700981847310012No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA291850.16835607289226676No Hit
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA290660.1676696116048184Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG286070.16502183235323198No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAA254200.14663736073056094No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG246030.14192442903438202No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA245560.14165330566875117No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAA237000.1367153992649211No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAA231800.13371573649623927No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA231250.1333984644726287No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA224850.1297065718342511No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA222460.12832788067710696No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAA218980.12632041405498914No Hit
AATAAAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA215100.1240822041429727Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAA214360.1236553290566603No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAAAA209330.12075373218618539No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA209230.12069604636371072No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA203600.11744833455838792No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAA203460.11736757440692341No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAA188310.1086281723020139No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAA180780.10428442986967273No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAA178500.10296919311725071No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG206.313928E-446.011
GAAGCAG718100.041.900299
GGAAGCA779100.041.5511478
AAGCAGT686450.041.29579510
CGGTAAT3900.040.10256237
GGGAAGC851000.039.862167
GCGATTA355.3774947E-639.42857438
ATACACG657.2759576E-1238.92307742
AGGGACG435650.038.4398046
AAGGGAC583500.037.6987155
AGGGGTG733450.037.627246
TAAAGTC20750.037.5759053
GAAGCAT41850.037.4814839
GAACAGT57050.037.453119
AAGCATG33600.037.4434510
AGGGCGC396850.037.3529056
TAAAGAT235950.037.2756923
ATAAAGT78700.037.2617532
GCGGTAA5100.036.98039236
ATAAAGA324600.036.9162062
Produced by FastQC (version 0.11.5)