FastQCFastQC Report
Fri 17 Jun 2016
SRR1527370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19425324
Sequences flagged as poor quality0
Sequence length52
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG2900671.493241502689994No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1414820.7283379160110791No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA1165100.5997840756735898No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA952220.49019516997502843Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA852200.43870568130549586Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA845420.43521539203155635No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA801460.4125851388630635No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA727940.374737636293737No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG726950.37422799228471043No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA695210.3578884964801617No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600860.30931787804414484No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA545020.2805718967673332Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA507010.26100465557228286No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA504130.259522054818751Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA490730.2526238429794015Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA486250.25031757514057423No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410880.21151770750387483No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA398070.20492322290222806Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373150.1920946080487512No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA373010.19202253717878784No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA372860.1919453183895414No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA367080.18896982104391155No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA309600.15937958100467206No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA307180.15813378453816265No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG307170.15812863661887955No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA298450.15363965100401927Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAA289510.1490374111649309No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG283830.146113393012132No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA282240.14529487384611964No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA278480.1433592561956753No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAA273510.14080074031197626No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAA258030.13283176126174268No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGGAG257210.13240963188052873No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAA255820.1316940711001783No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA253060.13027324537804363No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAA244630.12593354942239315No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA243230.12521284072275965No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA235870.1214239721304005Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAA235850.1214136762918343No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAA234150.12053853001370789No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAA228730.1177483577622695No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAA222980.11478830417448893No Hit
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA221600.11407789131342158Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA216320.11135978993194658No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAAAA210060.10813719246072807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG1102050.042.191199
AAGCAGT1071050.041.748110
GGAAGCA1184400.041.7083788
GGGAAGC1269500.040.38187
CGGTAAT5050.038.71287537
AGGGACG549850.037.9645356
AGGGGTG831450.037.217156
AAGGGAC724450.037.0628785
ATAAATT17900.036.8770942
AGCAGTG1178550.036.74574311
GCAGTGG1134550.036.5490312
AGGGCGC496800.036.175936
GAACAGT67050.036.1208089
TAAAGAT226000.036.0977863
AGGGTCA93950.035.7913786
GAAGCAT55400.035.7454879
ATAAAGT86300.035.5793722
AAGCATG44750.035.36089310
TGATGAA7850.035.15923740
AGGGAAC1045550.035.0296026
Produced by FastQC (version 0.11.5)