SRR1527369_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620551
Sequences flagged as poor quality	0
Sequence length	50
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	15081	2.430259559649408	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	9465	1.5252573922207846	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC	4121	0.6640872385992449	No Hit
ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4076	0.6568356186679258	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3849	0.6202552247921606	No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3263	0.5258230185754273	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2871	0.46265335161815874	No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2694	0.4341303132216369	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2537	0.40883021701681244	No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2354	0.37934029596278146	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTCGTTAAGAGCATTAACTGCT	2196	0.35387905264837216	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2006	0.32326110182724704	No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1835	0.2957049460882345	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1705	0.27475582184220154	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCT	1566	0.25235637360990476	No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1551	0.2499391669661317	No Hit
ATTCCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1270	0.20465682917278355	No Hit
ATTCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1240	0.19982241588523747	No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	1220	0.1965994736935401	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCT	1218	0.19627717947437034	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	1148	0.18499688180342952	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	1064	0.17146052459830052	No Hit
ATTCCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1020	0.1643700517765663	No Hit
ATTCCAGGGAGTATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTT	959	0.1545400780918893	No Hit
ATTCCAGGGGGGTGTGGTGGTGCACGCCTTTAATCCCAGCACTGGGGAGG	915	0.14744960527015508	No Hit
ATTCCAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	846	0.13633045470879912	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCATCCGACTGC	822	0.13246292407876226	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTG	818	0.13181833564042278	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	789	0.12714506946246157	No Hit
ATTCCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	718	0.1157036246819359	No Hit
ATTCCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	673	0.1084520047506168	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGAGTGCT	672	0.10829085764103193	No Hit
ATTCCAGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGG	644	0.10377873857265558	No Hit
ATTCCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	635	0.10232841458639177	No Hit
ATTCCAGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCG	628	0.10120038481929769	No Hit
ATTCCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	628	0.10120038481929769	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	20	7.8536774E-4	44.000004	35
ATTAGTG	20	7.8536774E-4	44.000004	40
ATTAGGC	20	7.8536774E-4	44.000004	24
CGAAACT	20	7.8536774E-4	44.000004	31
GCGAATC	20	7.8536774E-4	44.000004	42
CTATCGA	20	7.8536774E-4	44.000004	38
TATATTC	20	7.8536774E-4	44.000004	39
ATATGCG	20	7.8536774E-4	44.000004	15
ACTTATT	20	7.8536774E-4	44.000004	17
TAACGCT	20	7.8536774E-4	44.000004	20
ACTTAGC	20	7.8536774E-4	44.000004	24
TATGCGG	20	7.8536774E-4	44.000004	16
CGTTCAC	20	7.8536774E-4	44.000004	28
TACGCCT	40	8.301868E-9	44.000004	29
TACGCCA	20	7.8536774E-4	44.000004	35
CTACGTC	40	8.301868E-9	44.000004	14
GTTCGAA	20	7.8536774E-4	44.000004	33
CGAATCT	20	7.8536774E-4	44.000004	14
CGAATAC	20	7.8536774E-4	44.000004	21
TTAGTGA	20	7.8536774E-4	44.000004	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content