FastQCFastQC Report
Fri 17 Jun 2016
SRR1527368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583369
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC123512.117184834984375No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT87511.500079709412053No Hit
ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38950.6676734622511652No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC38360.6575597949153966No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC36200.6205334873810573No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31280.5361957868861732No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC28190.4832275969412156No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG24750.42425977383097147No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT22530.38620495775401165No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT19670.33717938388909935No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT19140.3280942250959513No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT18440.31609495876537835No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17900.3068383818817935No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTCGTTAAGAGCATTAACTGCTCT17510.30015307635476No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC14900.2554129547507667No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCTCT13230.2267861336478284No Hit
ATTCCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12270.2103299969658998No Hit
ATTCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT11560.1981593125448901No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT10840.18581721003344367No Hit
ATTCCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT10530.18050324922990424No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT9910.16987532762282534No Hit
ATTCCAGGGGGGTGTGGTGGTGCACGCCTTTAATCCCAGCACTGGGGAGGCA9280.15907598792530972No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8350.1431341055146914No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCATCCGACTGCTC8240.14124850651988707No Hit
ATTCCAGGGAGTATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAA8240.14124850651988707No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGCT7740.13267760199804926No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT7630.13079200300324495No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC7620.13062058491280817No Hit
ATTCCAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC7560.12959207637018766No Hit
ATTCCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG7270.12462095174752173No Hit
ATTCCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA6890.11810706431092499No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGAGTGCTCT6320.10833623315602987No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT6260.10730772461340936No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT6230.10679347034209909No Hit
ATTCCAGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG6180.1059363798899153No Hit
ATTCCAGGGGGGCGTGGTGGCGCACGCCTTTAATGCCAGCACTCGGGAGGCA6060.10387936280467423No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG5980.10250801808118018No Hit
ATTCCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT5890.10096525526724937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT351.0184158E-746.00000425
AGACGAT351.0184158E-746.00000443
ATATACC351.0184158E-746.00000415
GCATAAT206.3088856E-446.09
ATTTACG501.6370905E-1146.020
TAGAGAC405.6024874E-946.027
GTACCTA206.3088856E-446.09
ACACGCG301.8598148E-646.011
ATCTAAT206.3088856E-446.015
CCGATTT253.4150715E-546.030
TAGACGT206.3088856E-446.024
GAACTCG206.3088856E-446.018
TAGCGAT206.3088856E-446.029
ACCGATT206.3088856E-446.043
CGATTTT253.4150715E-546.031
ATTCGAT206.3088856E-446.037
ATTCGAC501.6370905E-1146.015
AAGGTAT253.4150715E-546.010
CGACATT206.3088856E-446.024
CGATTGT206.3088856E-446.045
Produced by FastQC (version 0.11.5)