##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583369
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80608842773613	31.0	31.0	34.0	30.0	34.0
2	32.38810255601515	33.0	31.0	34.0	31.0	34.0
3	32.36930999076056	34.0	31.0	34.0	31.0	34.0
4	36.293815749551314	37.0	37.0	37.0	35.0	37.0
5	36.48798616313174	37.0	37.0	37.0	35.0	37.0
6	35.91945921020829	37.0	35.0	37.0	35.0	37.0
7	36.50868489755198	37.0	37.0	37.0	35.0	37.0
8	36.70645680521248	37.0	37.0	37.0	35.0	37.0
9	38.72784806871808	39.0	39.0	39.0	39.0	39.0
10	38.006402465677816	39.0	39.0	39.0	35.0	39.0
11	37.93486283981494	39.0	38.0	39.0	35.0	39.0
12	37.238766543988454	39.0	35.0	39.0	35.0	39.0
13	37.19263279330921	39.0	35.0	39.0	35.0	39.0
14	37.8692662791475	40.0	36.0	40.0	35.0	40.0
15	37.91134942034973	40.0	36.0	40.0	35.0	40.0
16	38.0071429918285	40.0	36.0	40.0	35.0	40.0
17	37.78067741000979	40.0	36.0	40.0	34.0	40.0
18	37.70381353825795	40.0	35.0	40.0	34.0	40.0
19	37.572186729154275	40.0	35.0	40.0	33.0	40.0
20	37.52082472671671	40.0	35.0	40.0	33.0	40.0
21	37.48272534193624	40.0	35.0	40.0	33.0	40.0
22	37.54585348210138	40.0	35.0	40.0	33.0	40.0
23	37.52186866288747	40.0	35.0	40.0	34.0	40.0
24	37.543949027116625	39.0	35.0	40.0	34.0	40.0
25	37.61650516225579	39.0	35.0	40.0	34.0	40.0
26	37.53160006788156	39.0	35.0	40.0	34.0	40.0
27	37.516061017983475	39.0	35.0	40.0	34.0	40.0
28	37.34591142141595	39.0	35.0	40.0	34.0	40.0
29	37.278710387422024	39.0	35.0	40.0	34.0	40.0
30	37.15414086110164	39.0	35.0	40.0	33.0	40.0
31	37.11697227655223	39.0	35.0	40.0	33.0	40.0
32	37.09008363488633	39.0	35.0	40.0	33.0	40.0
33	36.946102724004874	39.0	35.0	40.0	33.0	40.0
34	36.28099367638664	38.0	35.0	40.0	31.0	40.0
35	36.25014870519345	38.0	35.0	40.0	31.0	40.0
36	36.339143835205505	38.0	35.0	40.0	31.0	40.0
37	36.38990244596473	38.0	35.0	40.0	32.0	40.0
38	36.4927584427695	38.0	35.0	40.0	32.0	40.0
39	36.516667838023615	38.0	35.0	40.0	32.0	40.0
40	36.40321648904896	38.0	35.0	40.0	32.0	40.0
41	36.330266092301784	38.0	35.0	40.0	32.0	40.0
42	36.2051497422729	38.0	35.0	40.0	32.0	40.0
43	36.15858230382485	38.0	35.0	40.0	32.0	40.0
44	36.05543832462815	38.0	35.0	40.0	32.0	40.0
45	35.924762885926405	37.0	35.0	40.0	31.0	40.0
46	35.68683629058109	37.0	35.0	40.0	31.0	40.0
47	35.65645071987027	37.0	35.0	40.0	31.0	40.0
48	35.47461555207767	37.0	34.0	40.0	30.0	40.0
49	35.36392232017814	36.0	34.0	40.0	30.0	40.0
50	35.73761547151117	36.0	35.0	40.0	31.0	40.0
51	35.840538321371206	36.0	35.0	40.0	32.0	40.0
52	35.522593761410015	36.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	6.0
14	7.0
15	20.0
16	28.0
17	64.0
18	120.0
19	200.0
20	286.0
21	401.0
22	614.0
23	906.0
24	1280.0
25	1783.0
26	2429.0
27	3120.0
28	3829.0
29	5249.0
30	6826.0
31	9553.0
32	13927.0
33	23239.0
34	69824.0
35	52499.0
36	48739.0
37	83621.0
38	198175.0
39	56618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.30832800508769	0.5843642702989017	0.05828215074849709	0.04902557386491226
8	98.95074986843662	0.8579475426359645	0.09805114772982453	0.09325144119759535
9	97.88367225546781	1.3646594179670157	0.32140891956891776	0.430259406996258
10	65.43165646443332	26.65191328301641	2.751260351509936	5.165169901040336
11	45.50944599387351	19.53480558617273	17.930160841594255	17.025587578359495
12	38.41102286888744	14.858862915238896	23.723920880266178	23.00619333560748
13	28.42746186376033	15.229468826763162	24.378223731463276	31.96484557801323
14	26.325018984553516	15.570419408641872	32.14894860714231	25.955612999662307
15	31.30951421827351	16.25952013219763	28.4343185873778	23.99664706215106
16	37.40685569510892	17.66566272805034	23.379199100397862	21.548282476442868
17	33.75085066227379	19.97706425949956	23.95773515562191	22.31434992260473
18	35.029286780751114	19.797589518812277	22.99659392254302	22.17652977789358
19	31.095927277589315	25.614319581602725	19.5766316002393	23.713121540568665
20	33.55166284118628	24.247603146550468	20.47229112277135	21.7284428894919
21	31.497045609211323	25.792594395656955	22.573705493435543	20.13665450169618
22	29.744467052585925	21.254300451343834	28.119594973335914	20.881637522734326
23	34.63759644410313	19.481323141956462	23.800201930510532	22.08087848342987
24	36.728382893160244	18.636917628465003	21.911517410078353	22.723182068296396
25	30.22066650781924	17.844794632556752	19.600630132900445	32.333908726723564
26	25.339022128361293	19.72885086454714	26.28096453531127	28.651162471780296
27	24.472846517384365	17.886277810442447	24.182807108365374	33.458068563807814
28	25.674830167526903	25.384962176598346	20.536058652413825	28.404149003460933
29	32.441387869427416	22.828261357734128	19.755592086655273	24.974758686183186
30	29.1921579651987	18.92832838220749	25.81265031223805	26.066863340355763
31	34.12299933661199	18.73068332393391	23.33548748733649	23.810829852117614
32	38.62495264575252	18.87861713598083	21.082025270454892	21.414404947811764
33	37.38697119661826	17.7386868345764	23.16629783207541	21.708044136729924
34	28.971885718987465	20.603768798136343	29.95788257517969	20.4664629076965
35	25.39833278765241	28.975656916977076	27.150054253825623	18.47595604154489
36	26.229881944361118	32.45321571766755	20.38983902127127	20.927063316700064
37	32.81542214276041	27.79750038140525	18.730854742024345	20.65622273380999
38	30.618013641451636	30.60772855602543	19.02980789174605	19.744449910776886
39	34.327158282322166	23.93785065713125	19.209111214342894	22.52587984620369
40	28.79961053809853	21.51759863825469	18.652859510875622	31.029931312771165
41	23.845627724476277	27.803328596480103	19.189398133942667	29.161645545100956
42	22.618274196949102	22.72198214166334	21.01671497799849	33.64302868338907
43	23.604613889322195	20.14316838913278	23.257149420006893	32.99506830153813
44	26.872014111137204	19.41172739723914	21.88220491661367	31.834053575009985
45	30.05319103346252	21.323210523699405	21.71095824426735	26.912640198570713
46	28.26770020347327	25.74888278259558	19.968836191158598	26.014580822772547
47	25.140177143454657	21.4029199357525	24.381480675181574	29.07542224561127
48	27.074801712123886	19.8536432343851	26.92789640861959	26.143658644871426
49	30.326260051528276	18.655773618413047	24.625580035963516	26.39238629409516
50	25.789337451938653	18.848276133973524	26.12291705592858	29.23946935815924
51	22.97979494968022	18.766681122925625	27.800071652761805	30.453452274632355
52	23.044762405955748	20.25887560017759	28.53888362254422	28.15747837132244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	28.0
6	48.0
7	33.5
8	19.0
9	130.5
10	242.0
11	273.0
12	304.0
13	459.5
14	461.0
15	307.0
16	516.5
17	726.0
18	666.5
19	607.0
20	686.0
21	765.0
22	913.0
23	1061.0
24	1474.5
25	1888.0
26	2059.5
27	2231.0
28	2930.5
29	3630.0
30	3865.0
31	4100.0
32	4726.0
33	5352.0
34	5940.0
35	6528.0
36	7544.5
37	8561.0
38	9094.5
39	10344.0
40	11060.0
41	11453.5
42	11847.0
43	13728.0
44	15609.0
45	16565.5
46	17522.0
47	19711.0
48	21900.0
49	22703.0
50	23506.0
51	29172.5
52	34839.0
53	35183.0
54	35527.0
55	41277.5
56	47028.0
57	52436.0
58	57844.0
59	59039.5
60	60235.0
61	65522.5
62	70810.0
63	55496.0
64	33463.5
65	26745.0
66	21352.5
67	15960.0
68	13788.0
69	11616.0
70	12258.0
71	12900.0
72	11732.0
73	10564.0
74	8435.5
75	6307.0
76	4718.0
77	3129.0
78	2059.0
79	989.0
80	725.0
81	461.0
82	291.5
83	122.0
84	82.5
85	43.0
86	22.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	583369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.802200665445026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.06014025147334	6.573369513978289
2	9.348932375006559	1.8328022229497969
3	3.352394942552857	0.9858254381017846
4	1.549411538394278	0.6075057125078639
5	0.8429078572302956	0.41311759795258235
6	0.6348040501547663	0.37334860097125494
7	0.5228826749208682	0.35877806328413064
8	0.4529318153996817	0.3551782833849587
9	0.4739170732560376	0.4180887225752482
>10	11.650315653253589	30.08576047064551
>50	2.603920745676162	17.174035644677726
>100	1.416504905304024	27.018405160370197
>500	0.0559606876169491	3.5654962810845277
>1k	0.031477886784533865	6.621023743119707
>5k	0.0017487714880296593	1.500079709412053
>10k+	0.0017487714880296593	2.117184834984375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12351	2.117184834984375	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8751	1.500079709412053	No Hit
ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3895	0.6676734622511652	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	3836	0.6575597949153966	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3620	0.6205334873810573	No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3128	0.5361957868861732	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2819	0.4832275969412156	No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2475	0.42425977383097147	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2253	0.38620495775401165	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1967	0.33717938388909935	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	1914	0.3280942250959513	No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1844	0.31609495876537835	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1790	0.3068383818817935	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTCGTTAAGAGCATTAACTGCTCT	1751	0.30015307635476	No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1490	0.2554129547507667	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCTCT	1323	0.2267861336478284	No Hit
ATTCCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1227	0.2103299969658998	No Hit
ATTCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	1156	0.1981593125448901	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT	1084	0.18581721003344367	No Hit
ATTCCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1053	0.18050324922990424	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	991	0.16987532762282534	No Hit
ATTCCAGGGGGGTGTGGTGGTGCACGCCTTTAATCCCAGCACTGGGGAGGCA	928	0.15907598792530972	No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	835	0.1431341055146914	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCATCCGACTGCTC	824	0.14124850651988707	No Hit
ATTCCAGGGAGTATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAA	824	0.14124850651988707	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGCT	774	0.13267760199804926	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	763	0.13079200300324495	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	762	0.13062058491280817	No Hit
ATTCCAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	756	0.12959207637018766	No Hit
ATTCCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	727	0.12462095174752173	No Hit
ATTCCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	689	0.11810706431092499	No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGAGTGCTCT	632	0.10833623315602987	No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT	626	0.10730772461340936	No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	623	0.10679347034209909	No Hit
ATTCCAGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG	618	0.1059363798899153	No Hit
ATTCCAGGGGGGCGTGGTGGCGCACGCCTTTAATGCCAGCACTCGGGAGGCA	606	0.10387936280467423	No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	598	0.10250801808118018	No Hit
ATTCCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	589	0.10096525526724937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03925474271001716	0.0	0.0	0.0	0.0
7	0.04302594069962579	0.0	0.0	0.0	0.0
8	0.0445687035135566	0.0	0.0	0.0	0.0
9	0.04542579396574038	0.0	0.0	0.0	0.0
10	0.055368043211072235	0.0	0.0	0.0	0.0
11	0.06668163717989814	0.0	0.0	0.0	0.0
12	0.07645246833479324	0.0	0.0	0.0	0.0
13	0.0821092653192062	0.0	0.0	0.0	0.0
14	0.09085158793148077	0.0	0.0	0.0	0.0
15	0.10250801808118018	0.0	0.0	0.0	0.0
16	0.11073608642214447	0.0	0.0	0.0	0.0
17	0.11982124521529255	0.0	0.0	0.0	0.0
18	0.12873498591800386	0.0	0.0	0.0	0.0
19	0.14244843315294436	0.0	0.0	0.0	0.0
20	0.15461911757395405	0.0	0.0	0.0	0.0
21	0.16233293164360807	0.0	0.0	0.0	0.0
22	0.1685039828993313	0.0	0.0	0.0	0.0
23	0.1767320512402956	0.0	0.0	0.0	0.0
24	0.18393161103863936	0.0	0.0	0.0	0.0
25	0.19027408038479932	0.0	0.0	0.0	0.0
26	0.19850214872576363	0.0	0.0	0.0	0.0
27	0.20415894571017656	0.0	0.0	0.0	0.0
28	0.21461544922681872	0.0	0.0	0.0	0.0
29	0.22112933666341544	0.0	0.0	0.0	0.0
30	0.2290145688235062	0.0	0.0	0.0	0.0
31	0.23672838289316026	0.0	0.0	0.0	0.0
32	0.24564212359587156	0.0	0.0	0.0	0.0
33	0.25232742912290507	0.0	0.0	0.0	0.0
34	0.2607269155543061	0.0	0.0	0.0	0.0
35	0.27495461706055685	0.0	0.0	0.0	0.0
36	0.30032449444519677	0.0	0.0	0.0	0.0
37	0.31558070449406805	0.0	0.0	0.0	0.0
38	0.32775138891507777	0.0	0.0	0.0	0.0
39	0.3370079657986626	0.0	0.0	0.0	0.0
40	0.34420752559700635	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATT	35	1.0184158E-7	46.000004	25
AGACGAT	35	1.0184158E-7	46.000004	43
ATATACC	35	1.0184158E-7	46.000004	15
GCATAAT	20	6.3088856E-4	46.0	9
ATTTACG	50	1.6370905E-11	46.0	20
TAGAGAC	40	5.6024874E-9	46.0	27
GTACCTA	20	6.3088856E-4	46.0	9
ACACGCG	30	1.8598148E-6	46.0	11
ATCTAAT	20	6.3088856E-4	46.0	15
CCGATTT	25	3.4150715E-5	46.0	30
TAGACGT	20	6.3088856E-4	46.0	24
GAACTCG	20	6.3088856E-4	46.0	18
TAGCGAT	20	6.3088856E-4	46.0	29
ACCGATT	20	6.3088856E-4	46.0	43
CGATTTT	25	3.4150715E-5	46.0	31
ATTCGAT	20	6.3088856E-4	46.0	37
ATTCGAC	50	1.6370905E-11	46.0	15
AAGGTAT	25	3.4150715E-5	46.0	10
CGACATT	20	6.3088856E-4	46.0	24
CGATTGT	20	6.3088856E-4	46.0	45
>>END_MODULE