FastQCFastQC Report
Fri 17 Jun 2016
SRR1527367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716283
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC147972.065803599973753No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT105811.477209427000222No Hit
ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47230.6593762521238113No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC47160.6583989847588174No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC43100.6017174775891652No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38440.5366593930052787No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC33370.46587731385499864No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG32460.4531728381100766No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT27450.38322841670122004No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT24710.34497537984288335No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT23130.32291705931873294No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT22650.3162157973873455No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTCGTTAAGAGCATTAACTGCTCT22050.3078392199731112No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA20710.28913153041465456No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC19220.2683296965026393No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCTCT16700.2331480713628552No Hit
ATTCCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15270.2131838951922634No Hit
ATTCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14700.20522614664874078No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT13280.1854015801017196No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT12900.18009641440603782No Hit
ATTCCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT12360.17255749473322696No Hit
ATTCCAGGGAGTATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAA12100.16892764452039208No Hit
ATTCCAGGGGGGTGTGGTGGTGCACGCCTTTAATCCCAGCACTGGGGAGGCA11180.15608355915189945No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10230.1428206449126951No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCATCCGACTGCTC10020.1398888428177131No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC9580.13374601938060796No Hit
ATTCCAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC9540.13318758088632565No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGCT9200.1284408536849262No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT8910.12439217460137961No Hit
ATTCCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA7980.11140847960931642No Hit
ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT7620.10638253316077584No Hit
ATTCCAGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG7610.10624292353720527No Hit
ATTCCAGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGC7590.10596370429006412No Hit
ATTCCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG7510.10484682730149955No Hit
ATTCCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC7480.10442799843078782No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGAGTGCTCT7440.10386955993650555No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT7400.10331112144222325No Hit
ATTCCAGGGGGGCGTGGTGGCGCACGCCTTTAATGCCAGCACTCGGGAGGCA7290.10177541558294696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG551.8189894E-1246.019
CGGGTTA253.4158576E-546.018
AACGTAA206.309853E-446.031
TAGAGAC253.4158576E-546.027
AGATCGC405.6061253E-946.020
ATCTATC405.6061253E-946.042
GTCGATA301.8604151E-646.024
CAACGTA206.309853E-446.030
CCGATTT405.6061253E-946.030
TCCGATT301.8604151E-646.025
CCGATAT206.309853E-446.012
ATAATCC551.8189894E-1246.034
GGACTAA301.8604151E-646.08
ATTACGC206.309853E-446.026
ACATTCG253.4158576E-546.011
TTCGTGT253.4158576E-546.025
TAACGTA206.309853E-446.038
CGATTGC551.8189894E-1246.045
CGATTAC206.309853E-446.035
CACGACC453.092282E-1046.030
Produced by FastQC (version 0.11.5)