##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 716283 Sequences flagged as poor quality 0 Sequence length 52 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.312277409906418 31.0 30.0 33.0 27.0 34.0 2 31.27710695353652 31.0 31.0 33.0 30.0 34.0 3 31.25221455765389 31.0 31.0 33.0 30.0 34.0 4 35.72382005436399 37.0 35.0 37.0 35.0 37.0 5 36.080949848034926 37.0 35.0 37.0 35.0 37.0 6 35.15452830794532 36.0 35.0 37.0 33.0 37.0 7 36.21443898570816 37.0 35.0 37.0 35.0 37.0 8 36.53739234352902 37.0 37.0 37.0 35.0 37.0 9 38.61268939790558 39.0 39.0 39.0 38.0 39.0 10 37.53237337756166 39.0 37.0 39.0 35.0 39.0 11 37.32619509328017 39.0 37.0 39.0 34.0 39.0 12 36.64233131318208 38.0 35.0 39.0 33.0 39.0 13 36.55231521619248 39.0 35.0 39.0 33.0 39.0 14 37.219138245637545 39.0 35.0 40.0 33.0 40.0 15 37.25609989347786 39.0 35.0 40.0 33.0 40.0 16 37.34272906099963 39.0 35.0 40.0 33.0 40.0 17 37.09254303117623 39.0 35.0 40.0 32.0 40.0 18 37.043877908591995 39.0 35.0 40.0 32.0 40.0 19 36.87065587205057 39.0 35.0 40.0 32.0 40.0 20 36.775585906687716 39.0 35.0 40.0 31.0 40.0 21 36.81452721899026 39.0 35.0 40.0 31.0 40.0 22 36.90908621313084 39.0 35.0 40.0 32.0 40.0 23 36.9880508123186 39.0 35.0 40.0 32.0 40.0 24 37.03934478411466 39.0 35.0 40.0 33.0 40.0 25 37.09482425242537 39.0 35.0 40.0 33.0 40.0 26 36.96314864376231 39.0 35.0 40.0 33.0 40.0 27 36.91857268705246 39.0 35.0 40.0 33.0 40.0 28 36.68960871610802 38.0 35.0 40.0 32.0 40.0 29 36.655648675174476 38.0 35.0 40.0 32.0 40.0 30 36.59515861747382 38.0 35.0 40.0 32.0 40.0 31 36.53783909432445 38.0 35.0 40.0 32.0 40.0 32 36.460426954150805 38.0 35.0 40.0 32.0 40.0 33 36.28961318361597 38.0 35.0 40.0 31.0 40.0 34 35.597726038451285 38.0 34.0 40.0 28.0 40.0 35 35.594249758824375 38.0 34.0 40.0 29.0 40.0 36 35.80838579164939 38.0 34.0 40.0 30.0 40.0 37 35.93773550398376 38.0 34.0 40.0 31.0 40.0 38 35.871171031561545 38.0 34.0 40.0 31.0 40.0 39 35.8422662550975 37.0 34.0 40.0 31.0 40.0 40 36.06842686480064 38.0 35.0 40.0 31.0 40.0 41 36.16597769317435 38.0 35.0 40.0 32.0 40.0 42 36.18448713706733 38.0 35.0 40.0 32.0 40.0 43 36.13560282737409 38.0 35.0 40.0 32.0 40.0 44 36.02695443002277 37.0 35.0 40.0 31.0 40.0 45 35.91964070067278 37.0 35.0 40.0 31.0 40.0 46 35.63832172479314 37.0 35.0 40.0 31.0 40.0 47 35.61841618466444 37.0 35.0 40.0 31.0 40.0 48 35.53544618537645 36.0 34.0 40.0 31.0 40.0 49 35.415649959583014 36.0 34.0 40.0 30.0 40.0 50 35.33150444726456 36.0 34.0 40.0 30.0 40.0 51 35.24289003089561 35.0 34.0 40.0 30.0 40.0 52 34.82760305633388 35.0 34.0 39.0 29.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 0.0 12 5.0 13 11.0 14 10.0 15 22.0 16 46.0 17 85.0 18 143.0 19 247.0 20 445.0 21 646.0 22 1027.0 23 1540.0 24 2229.0 25 3074.0 26 4264.0 27 5448.0 28 7052.0 29 9439.0 30 12801.0 31 17191.0 32 24126.0 33 39481.0 34 93637.0 35 57184.0 36 70862.0 37 116918.0 38 218102.0 39 30246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.32959458761411 0.5722598470157745 0.06184706324176339 0.03629850212834871 8 98.96688878557777 0.8443590033548193 0.09088586494444235 0.09786634612297095 9 97.87625840624446 1.3656613377673348 0.3111898509388049 0.44689040504940086 10 65.36745950971893 26.794995832652734 2.67757297046 5.15997168716834 11 45.38555291693367 19.633022143482396 18.005732371143807 16.975692568440127 12 38.08438843306347 14.912681160937785 23.83987893053444 23.163051475464307 13 28.266062436215854 15.421837458099663 24.46756379810773 31.844536307576753 14 26.336098999976265 15.495272120097784 32.22455370293585 25.9440751769901 15 31.217130659250603 16.421163143617814 28.36462683045668 23.997079366674903 16 37.3621878503329 17.69956846665354 23.385589215435797 21.55265446757776 17 33.685847632849026 20.076422307942533 23.84699902133654 22.3907310378719 18 35.047181072285674 19.81772567546626 22.910218447178003 22.224874805070062 19 30.957875588280054 25.661226079636123 19.743453355726718 23.63744497635711 20 33.51775764607006 24.170334909525984 20.441082644708867 21.870824799695093 21 31.337892983639147 25.871617782356974 22.672044429366604 20.118444804637274 22 29.60017199905624 21.322577807933456 28.06125511843782 21.01599507457248 23 34.526437176367445 19.54171744966724 23.73461327436223 22.197232099603088 24 36.661905978502915 18.674462468046848 21.927645916488313 22.735985636961928 25 29.990380896935985 17.920151671895045 19.696684131830576 32.39278329933839 26 25.334260341233843 19.69137896613489 26.26894677103882 28.70541392159244 27 24.495625332445417 17.995122039752445 24.10122814585855 33.408024481943585 28 25.66066764114184 25.44608764971387 20.634023144483397 28.259221564660898 29 32.51954883754047 22.825754624917806 19.687609506298486 24.96708703124324 30 29.223644844286405 18.88317885528485 25.841043274795016 26.052133025633722 31 34.039478809353284 18.85818873266572 23.294424131244217 23.80790832673678 32 38.55333715863702 19.002963912308406 21.178221457161488 21.265477471893092 33 37.29336030591261 17.81544445421712 23.170171566266408 21.721023673603867 34 28.85158519747083 20.812583853030155 29.83401811853695 20.501812830962063 35 25.234858289251594 29.084593659210118 27.034286727452695 18.646261324085593 36 26.191044601086443 32.3514588507615 20.458673457278756 20.9988230908733 37 32.72756717666062 27.822243442885004 18.713413553023038 20.736775827431337 38 30.549098610465414 30.56166347658677 18.99472694451774 19.894510968430076 39 34.18662735259667 24.080286702322965 19.14620338609181 22.586882558988556 40 28.700667194391045 21.557261585155587 18.727793344250806 31.014277876202563 41 23.65475656967986 27.956687510383464 19.27994940547242 29.108606514464253 42 22.31185160055453 22.763069903934618 21.057040303902227 33.868038191608626 43 23.48401400005305 20.10043516319667 23.32220644633476 33.09334439041552 44 26.739151983224506 19.576480245936313 21.873896211413644 31.810471559425537 45 29.86305692023963 21.596073060508207 21.71153021920107 26.8293398000511 46 28.23716324413674 25.723073142877883 20.02518557609213 26.014578036893237 47 25.274367812722065 21.28390594220441 24.286350506713127 29.155375738360394 48 27.0665365504975 19.937650342113383 26.95903714034816 26.03677596704096 49 30.126779499164435 18.7216505208137 24.71188063935623 26.43968934066563 50 25.674628603498896 18.85372122471146 26.167590184326585 29.304059987463056 51 22.893465292349532 18.88499378039127 27.743922444061912 30.477618483197283 52 22.971646681549053 20.403388046344812 28.453837379918273 28.171127892187865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 6.0 4 12.0 5 27.0 6 42.0 7 33.5 8 25.0 9 138.0 10 251.0 11 293.5 12 336.0 13 544.5 14 548.0 15 343.0 16 553.5 17 764.0 18 776.0 19 788.0 20 870.5 21 953.0 22 1164.5 23 1376.0 24 1908.0 25 2440.0 26 2628.5 27 2817.0 28 3863.0 29 4909.0 30 4942.5 31 4976.0 32 5858.5 33 6741.0 34 7435.5 35 8130.0 36 9407.0 37 10684.0 38 11215.0 39 12497.0 40 13248.0 41 14008.0 42 14768.0 43 17203.0 44 19638.0 45 20554.5 46 21471.0 47 24333.0 48 27195.0 49 28004.0 50 28813.0 51 35894.0 52 42975.0 53 43310.0 54 43645.0 55 50842.0 56 58039.0 57 64532.5 58 71026.0 59 72141.5 60 73257.0 61 79932.0 62 86607.0 63 67729.0 64 40819.5 65 32788.0 66 26100.5 67 19413.0 68 16703.0 69 13993.0 70 15062.0 71 16131.0 72 14543.0 73 12955.0 74 10320.5 75 7686.0 76 5784.0 77 3882.0 78 2523.0 79 1164.0 80 825.0 81 486.0 82 297.5 83 109.0 84 74.5 85 40.0 86 27.0 87 14.0 88 7.0 89 1.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 716283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.432165778051413 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4670149938574 6.457922357503948 2 9.761548822545551 1.841450934895844 3 3.5286629860422436 0.9984880277767307 4 1.6725625730821037 0.6310354985389853 5 0.9176891993901808 0.43278983306877306 6 0.5787362531638075 0.3275241768965619 7 0.4455233048652329 0.2941574768631951 8 0.35967496040615143 0.2714011082211919 9 0.34931395331626236 0.29653084046389483 >10 9.178372137771792 24.034215526544674 >50 3.0994212637468364 19.95384505844757 >100 1.5319489054336082 29.08822350942295 >500 0.07252704962922396 4.424228970951425 >1k 0.03404330900963574 7.405173653430279 >5k 0.0 0.0 >10k+ 0.0029602877399683247 3.5430130269739757 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 14797 2.065803599973753 No Hit ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 10581 1.477209427000222 No Hit ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4723 0.6593762521238113 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC 4716 0.6583989847588174 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 4310 0.6017174775891652 No Hit ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 3844 0.5366593930052787 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 3337 0.46587731385499864 No Hit ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 3246 0.4531728381100766 No Hit ATTCCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT 2745 0.38322841670122004 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT 2471 0.34497537984288335 No Hit ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT 2313 0.32291705931873294 No Hit ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 2265 0.3162157973873455 No Hit ATTCCAGGGGGGCTGGAGAGATGGCTCAGTCGTTAAGAGCATTAACTGCTCT 2205 0.3078392199731112 No Hit ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 2071 0.28913153041465456 No Hit ATTCCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC 1922 0.2683296965026393 No Hit ATTCCAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCTCT 1670 0.2331480713628552 No Hit ATTCCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 1527 0.2131838951922634 No Hit ATTCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1470 0.20522614664874078 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT 1328 0.1854015801017196 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT 1290 0.18009641440603782 No Hit ATTCCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 1236 0.17255749473322696 No Hit ATTCCAGGGAGTATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAA 1210 0.16892764452039208 No Hit ATTCCAGGGGGGTGTGGTGGTGCACGCCTTTAATCCCAGCACTGGGGAGGCA 1118 0.15608355915189945 No Hit ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1023 0.1428206449126951 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCATCCGACTGCTC 1002 0.1398888428177131 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC 958 0.13374601938060796 No Hit ATTCCAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 954 0.13318758088632565 No Hit ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGCT 920 0.1284408536849262 No Hit ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT 891 0.12439217460137961 No Hit ATTCCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA 798 0.11140847960931642 No Hit ATTCCAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT 762 0.10638253316077584 No Hit ATTCCAGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG 761 0.10624292353720527 No Hit ATTCCAGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGC 759 0.10596370429006412 No Hit ATTCCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG 751 0.10484682730149955 No Hit ATTCCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 748 0.10442799843078782 No Hit ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGAGTGCTCT 744 0.10386955993650555 No Hit ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT 740 0.10331112144222325 No Hit ATTCCAGGGGGGCGTGGTGGCGCACGCCTTTAATGCCAGCACTCGGGAGGCA 729 0.10177541558294696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.03308748078622556 0.0 0.0 0.0 0.0 7 0.03629850212834871 0.0 0.0 0.0 0.0 8 0.037136159869772145 0.0 0.0 0.0 0.0 9 0.037834207987625004 0.0 0.0 0.0 0.0 10 0.04369781217758902 0.0 0.0 0.0 0.0 11 0.050957512603258766 0.0 0.0 0.0 0.0 12 0.059054870770351944 0.0 0.0 0.0 0.0 13 0.06533730383102768 0.0 0.0 0.0 0.0 14 0.07538919672810886 0.0 0.0 0.0 0.0 15 0.08488265113090776 0.0 0.0 0.0 0.0 16 0.09200274193300692 0.0 0.0 0.0 0.0 17 0.09940205198224725 0.0 0.0 0.0 0.0 18 0.10666175240791698 0.0 0.0 0.0 0.0 19 0.11727208379928045 0.0 0.0 0.0 0.0 20 0.12662592857850877 0.0 0.0 0.0 0.0 21 0.13416484825131966 0.0 0.0 0.0 0.0 22 0.1414245486769894 0.0 0.0 0.0 0.0 23 0.14728815286695343 0.0 0.0 0.0 0.0 24 0.1544082436690526 0.0 0.0 0.0 0.0 25 0.15803809388188747 0.0 0.0 0.0 0.0 26 0.16432052694256322 0.0 0.0 0.0 0.0 27 0.17102178887395067 0.0 0.0 0.0 0.0 28 0.18233016838316699 0.0 0.0 0.0 0.0 29 0.1897294784324073 0.0 0.0 0.0 0.0 30 0.19908332321163563 0.0 0.0 0.0 0.0 31 0.20578458514302309 0.0 0.0 0.0 0.0 32 0.21234623745083997 0.0 0.0 0.0 0.0 33 0.21793062239366284 0.0 0.0 0.0 0.0 34 0.22546954206647374 0.0 0.0 0.0 0.0 35 0.23719675044640176 0.0 0.0 0.0 0.0 36 0.25939468059412274 0.0 0.0 0.0 0.0 37 0.2708426697269096 0.0 0.0 0.0 0.0 38 0.2793588567647145 0.0 0.0 0.0 0.0 39 0.2885730919203723 0.0 0.0 0.0 0.0 40 0.2952743538517597 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAACG 55 1.8189894E-12 46.0 19 CGGGTTA 25 3.4158576E-5 46.0 18 AACGTAA 20 6.309853E-4 46.0 31 TAGAGAC 25 3.4158576E-5 46.0 27 AGATCGC 40 5.6061253E-9 46.0 20 ATCTATC 40 5.6061253E-9 46.0 42 GTCGATA 30 1.8604151E-6 46.0 24 CAACGTA 20 6.309853E-4 46.0 30 CCGATTT 40 5.6061253E-9 46.0 30 TCCGATT 30 1.8604151E-6 46.0 25 CCGATAT 20 6.309853E-4 46.0 12 ATAATCC 55 1.8189894E-12 46.0 34 GGACTAA 30 1.8604151E-6 46.0 8 ATTACGC 20 6.309853E-4 46.0 26 ACATTCG 25 3.4158576E-5 46.0 11 TTCGTGT 25 3.4158576E-5 46.0 25 TAACGTA 20 6.309853E-4 46.0 38 CGATTGC 55 1.8189894E-12 46.0 45 CGATTAC 20 6.309853E-4 46.0 35 CACGACC 45 3.092282E-10 46.0 30 >>END_MODULE