FastQCFastQC Report
Fri 17 Jun 2016
SRR1527366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208984
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG15180.7263713968533476No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC13220.6325843126746545No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11670.5584159552884431No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT11560.5531523944416797No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG9130.4368755502813612No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT7120.3406959384450484No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC6990.33447536653523713No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT6780.3244267503732343No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT6710.3210772116525667No Hit
CTGAAAGGGGGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGAGGCA6680.3196416950579949No Hit
CTGAAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT6410.30672204570684836No Hit
CTGAAAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6150.29428090188722583No Hit
CTGAAAGGGGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGG5930.28375378019369907No Hit
CTGAAAGGGGGTGCTGGAGAGATGGCTCAGAGGTTAAGAGAACTGTCTGC5370.25695747042835815No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT5270.2521724151131187No Hit
CTGAAAGGGGGAGGAGCGTCAACTGCGGCACCATGGTAGCTCAACCGAAG5100.24403782107721164No Hit
CTGAAAGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCT4720.22585461087930178No Hit
CTGAAAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT4620.22106955556406233No Hit
CTGAAAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC4420.21149944493358344No Hit
CTGAAAGGGCAGTGGGCAGTGATGGCATTACTCTGCACTCTAGCCACTTG4400.21054243387053553No Hit
CTGAAAGGGTGAGCACACAAAGGGCCAGAGTCAGAAAAGGAAGGGAACAA4260.20384335642920035No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC4220.20192933430310456No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG4190.20049381770853272No Hit
CTGAAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT4160.1990583011139609No Hit
CTGAAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG4100.1961872679248172No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC4080.1952302568617693No Hit
CTGAAAGGGAAGCAGGCTACCTGGCCCGCAGGTCATGTGTGCAAGAGTAG3930.18805267388891017No Hit
CTGAAAGGGGGCTGGCGCCCTTGCGAGGAGCCCTCTGCTGGTTCCACACT3910.18709566282586226No Hit
CTGAAAGGGGGTGGTCTCCCTGCCTCAGCCAGGCTACTGCTGGGACACGC3850.1842246296367186No Hit
CTGAAAGGGGTAGCCACATGGCCCTGGACTGGATTATGAAGGAGCGTGAA3850.1842246296367186No Hit
CTGAAAGGGGAAAAAAGCCTCATGATGATCATGAAGGCAATGTAGATTAT3780.180875090916051No Hit
CTGAAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG3730.17848256325843126No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC3680.17609003560081155No Hit
CTGAAAGGGATTGCGCAGGGGAGCCACGGAACTCACTCATGAGGACTACA3670.1756115300692876No Hit
CTGAAAGGGGAGTGGACCAGCGGGTCCAACACAGGGAAATCCAAGTCTGT3610.17274049688014392No Hit
CTGAAAGGGGTCTATCACATAAAAGCAAAAGGATTTTGTTCCTCAAGATG3460.16556291390728478No Hit
CTGAAAGGGGAGCCGGAGGGCAGCCATGGCGGCCGACAGCCGGGAGGAGA3410.16317038624966504No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC3350.1602993530605214No Hit
CTGAAAGGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACA3350.1602993530605214No Hit
CTGAAAGGGTCCGCCCCAGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCA3300.15790682540290166No Hit
CTGAAAGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGC3240.155035792213758No Hit
CTGAAAGGGGCCGCCGCCGCCACCCCGCCCTTGCCTCCGGTTCACGCTAA3240.155035792213758No Hit
CTGAAAGGGGGAGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCC3220.15407878115071008No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA3210.15360027561918616No Hit
CTGAAAGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTC3180.15216475902461432No Hit
CTGAAAGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAA3170.15168625349309037No Hit
CTGAAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCAC3160.15120774796156644No Hit
CTGAAAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC3140.15025073689851853No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTAC3080.1473797037093749No Hit
CTGAAAGGGAGGGATGTCCCTTTGAAGGCCTCTCTTGTTCTCCAGGCAGA3060.14642269264632699No Hit
CTGAAAGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGC3040.1454656815832791No Hit
CTGAAAGGGAGTGCTACTTCTTTTACAGACTACACTTTATAGAACTGATT3020.1445086705202312No Hit
CTGAAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG2960.14163763733108756No Hit
CTGAAAGGGGAATGAAAAGTAATCAAGCACTTGGGAAGCAGAGGCAGGTG2950.1411591317995636No Hit
CTGAAAGGGGGAGGGGAGGTGGGAGGCCCAGGGCTTTGCCTAGGGGTGAG2940.14068062626803965No Hit
CTGAAAGGGGGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGA2880.137809593078896No Hit
CTGAAAGGGGGGGCCTCGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGC2880.137809593078896No Hit
CTGAAAGGGGATAAAGATGGTGACGGCACCATCACAACCAAGGAACTGGG2830.13541706542127627No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA2830.13541706542127627No Hit
CTGAAAGGGACTGGCTGACCTTCCACCTGGTCCGGTGATCCCTGGTCCTT2770.1325460322321326No Hit
CTGAAAGGGAGCGAGCTGGCCTGGCTGGACCTTGTTCCCTGGGGGAGGGG2760.13206752670060867No Hit
CTGAAAGGGCAAAACGCTTTGTGTCTGGCAGAGTGGCGTGCTTCATGATC2750.1315890211690847No Hit
CTGAAAGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGC2730.13063201010603684No Hit
CTGAAAGGGGGGAAAAGGACCAAGATGGGGGCCGCAAGCTGACGCCTCAG2730.13063201010603684No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGC2710.12967499904298893No Hit
CTGAAAGGGACTCTGACTGGATTGAACGCTTAGCTTCAGCCTCATTCCTA2710.12967499904298893No Hit
CTGAAAGGGCAAGGAGATGCTGAAGCTCCTGGACTTTGGCAGTCTCTCTA2710.12967499904298893No Hit
CTGAAAGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAA2690.12871798797994105No Hit
CTGAAAGGGCTTTAAAATAGAAGGGGTTTCGTTTTTCTCTCTCTCTTGCT2690.12871798797994105No Hit
CTGAAAGGGGGTTCTTCCGGTCGCGAGCACCCCAGGGTGTAGAGGGCGGT2670.12776097691689314No Hit
CTGAAAGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGT2660.12728247138536922No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG2660.12728247138536922No Hit
CTGAAAGGGGATCTTCTTCTGGCTCTACGGCGATTAAGAAGAAACAGCAA2650.12680396585384526No Hit
CTGAAAGGGAGGACCCATATGGTGGAGGAGAGAATTGCTGCAAGTTGTCC2640.12632546032232134No Hit
CTGAAAGGGAACTACTGTCAGCACTTACGATGTATGTATGGATGTGAAAC2630.12584695479079738No Hit
CTGAAAGGGCAGAGTGCAGTGCTCTGAAAGGGGCCGAAGCCGCGCCCTAC2600.12441143819622554No Hit
CTGAAAGGGATCAGCTGGGATCACACCACTAGTCCCTGTCCTTTAACCCA2590.12393293266470161No Hit
CTGAAAGGGGTGTGTGTGGGTGATTGGATGTGGGGGTGGGGCCCGTGGAG2570.12297592160165371No Hit
CTGAAAGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGG2540.12154040500708188No Hit
CTGAAAGGGAGCTGGGCTGGAGGAGAGCTTCCGGAAGTTTGCCATCCATG2530.12106189947555794No Hit
CTGAAAGGGACCCAAGGGGGTGGGGCCTTGTGGGATCTCTGAGCCTGAAG2530.12106189947555794No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA2520.12058339394403399No Hit
CTGAAAGGGGGGGATACTATACAGAGGGGATCTTCCTGTGCCTTAAGGCA2500.11962638288098609No Hit
CTGAAAGGGATGTGCAACTGTAGTTATTTGTAATCTAATAATTTTCATTA2460.11771236075489033No Hit
CTGAAAGGGGGAAAGCATGGCTTAGAAGTAATTTATATGATTGAGCCCAT2420.11579833862879454No Hit
CTGAAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG2420.11579833862879454No Hit
CTGAAAGGGGAGTGGGTGGCTGCGGCCTGGGAGGGTGGCGACTCAGGGAG2400.11484132756574665No Hit
CTGAAAGGGGGGACTCCGATGGGTCGAAGTCGTAGCCGCTCCCCGCGTAG2400.11484132756574665No Hit
CTGAAAGGGAACGTCACGTCCTTCCCTCCCACTCACCAGGGAACTTTTTT2370.11340581097117482No Hit
CTGAAAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGC2360.11292730543965089No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT2350.11244879990812692No Hit
CTGAAAGGGCAAGTGTTACTGTCTCTCATTCCTCTGTCTCTGAGATCCCT2340.111970294376603No Hit
CTGAAAGGGACCACTTTCCGGAAGCTACAGGGGCTTGAGGTCATGAGAAC2330.11149178884507906No Hit
CTGAAAGGGAGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2330.11149178884507906No Hit
CTGAAAGGGCTGGGAGTCCTCAGCTGGGGGATCCTTCACAGTGAGGACTG2330.11149178884507906No Hit
CTGAAAGGGATTTCTCTAGATCTTAACATTTTCCCCAGCCTCCAAGTCCT2320.1110132833135551No Hit
CTGAAAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCC2310.11053477778203116No Hit
CTGAAAGGGGGTATCTGGGGCGGGAACGTCTTTACTCTTTCCTGTAACTT2310.11053477778203116No Hit
CTGAAAGGGAATTTTTAAAGCTCACATAGATATTACTTGAGGAGTGAAAC2280.10909926118745933No Hit
CTGAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG2260.10814225012441145No Hit
CTGAAAGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTC2240.10718523906136355No Hit
CTGAAAGGGGGAACTCTACCCTGGTAGGAAAGCACCAAAGCATGTGGGGA2220.10622822799831566No Hit
CTGAAAGGGAACGACCTCCCTCACTCCCCAGTGCCAGGGAAGTAACCCCT2220.10622822799831566No Hit
CTGAAAGGGAGACAGTAGGCAGTCTGAAGTCCACACCTGACAATGCGTGC2210.10574972246679172No Hit
CTGAAAGGGATGTCAGCTTTCTGGAGTCTCCATCAAGTTCTGCTGACCCT2200.10527121693526777No Hit
CTGAAAGGGGGTGCAAGCGCCCCGCCCGCTCAAATATGGGCTCCTGCGGG2200.10527121693526777No Hit
CTGAAAGGGAAGCAGTGGGAAGCATTAGTATCAACGCAGAGTAACTTCAG2190.10479271140374383No Hit
CTGAAAGGGGAACTCAGTGTGCTGACTCAGGTCAAGCAACAACTCAGCCT2190.10479271140374383No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCT2180.10431420587221989No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG2160.10335719480917199No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCT2160.10335719480917199No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG2160.10335719480917199No Hit
CTGAAAGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCC2160.10335719480917199No Hit
CTGAAAGGGGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCA2140.10240018374612411No Hit
CTGAAAGGGGTGCAGCCCTTCCCCCATAGGGACTGCTGTCCCCTGACCCC2140.10240018374612411No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG2110.10096466715155228No Hit
CTGAAAGGGAGGAAGAGCAGACAGAGGTCGAGGAGACCATTGAGGCTACG2110.10096466715155228No Hit
CTGAAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT2100.10048616162002832No Hit
CTGAAAGGGACTGCCCACTGTGAAACAAAGCTTTGACTAGGTTGTTTTGT2100.10048616162002832No Hit
CTGAAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGAT2090.10000765608850438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG650.044.00000424
GATTAAG650.044.00000432
ACAACTC302.517747E-644.039
TATCATG207.841684E-444.011
ATTTAGC207.841684E-444.038
TTGATGC302.517747E-644.030
GTTGACT207.841684E-444.020
TTCTATC302.517747E-644.021
TCGCCAA207.841684E-444.041
GTCATGC207.841684E-444.015
ATCCTAC207.841684E-444.014
AGCAATT254.4303062E-544.016
GAAATAA254.4303062E-544.043
CTATGAC207.841684E-444.037
TCGTTGA254.4303062E-544.024
TTGGCGG207.841684E-444.019
ATCTCGT408.256393E-944.010
TCGTTAG408.256393E-944.013
ACAAATC207.841684E-444.010
GTACCGC408.256393E-944.027
Produced by FastQC (version 0.11.5)