FastQCFastQC Report
Fri 17 Jun 2016
SRR1527365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177633
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC13100.737475581676828No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT11760.6620391481312594No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10010.5635214177545839No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG9940.5595807085395169No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9630.5421289963013629No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6420.3614193308675753No Hit
CTGAAAGGGGGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGAGGCAGA5960.33552324173999204No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5820.32764182330985797No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA5660.31863448796113336No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT5630.3169456125832475No Hit
CTGAAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA5620.31638265412395217No Hit
CTGAAAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5410.3045605264787511No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG4870.2741607696768056No Hit
CTGAAAGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG4620.2600868081944233No Hit
CTGAAAGGGGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGA4480.2522053897642893No Hit
CTGAAAGGGGGAGGAGCGTCAACTGCGGCACCATGGTAGCTCAACCGAAGAA4200.23644255290402122No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT4110.23137592677036362No Hit
CTGAAAGGGGGTGCTGGAGAGATGGCTCAGAGGTTAAGAGAACTGTCTGCTG3880.21842788220657197No Hit
CTGAAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA3870.2178649237472767No Hit
CTGAAAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC3800.21392421453220967No Hit
CTGAAAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG3790.21336125607291437No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA3780.2127982976136191No Hit
CTGAAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC3680.2071687130206662No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG3550.19985025304982748No Hit
CTGAAAGGGGGCTGGCGCCCTTGCGAGGAGCCCTCTGCTGGTTCCACACTAC3540.19928729459053215No Hit
CTGAAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC3500.19703546075335102No Hit
CTGAAAGGGGGTGGTCTCCCTGCCTCAGCCAGGCTACTGCTGGGACACGCAC3480.19590954383476045No Hit
CTGAAAGGGGGAGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCGG3460.19478362691616985No Hit
CTGAAAGGGGAGCCGGAGGGCAGCCATGGCGGCCGACAGCCGGGAGGAGAAA3390.19084291770110284No Hit
CTGAAAGGGATTGCGCAGGGGAGCCACGGAACTCACTCATGAGGACTACATG3360.18915404232321698No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC3250.1829614992709688No Hit
CTGAAAGGGTGAGCACACAAAGGGCCAGAGTCAGAAAAGGAAGGGAACAAGT3250.1829614992709688No Hit
CTGAAAGGGGTAGCCACATGGCCCTGGACTGGATTATGAAGGAGCGTGAATC3190.17958374851519707No Hit
CTGAAAGGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATT3170.1784578315966065No Hit
CTGAAAGGGAAGCAGGCTACCTGGCCCGCAGGTCATGTGTGCAAGAGTAGGA3030.17057641316647246No Hit
CTGAAAGGGGAGTGGACCAGCGGGTCCAACACAGGGAAATCCAAGTCTGTGC2980.16776162086999602No Hit
CTGAAAGGGGCCGCCGCCGCCACCCCGCCCTTGCCTCCGGTTCACGCTAAAG2970.1671986624107007No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2970.1671986624107007No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA2960.16663570395140542No Hit
CTGAAAGGGCAGTGGGCAGTGATGGCATTACTCTGCACTCTAGCCACTTGCC2960.16663570395140542No Hit
CTGAAAGGGGAAAAAAGCCTCATGATGATCATGAAGGCAATGTAGATTATGG2920.16438387011422426No Hit
CTGAAAGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGCTC2890.16269499473633842No Hit
CTGAAAGGGTCCGCCCCAGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCAAA2840.15988020243986198No Hit
CTGAAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCA2830.15931724398056668No Hit
CTGAAAGGGGTCTATCACATAAAAGCAAAAGGATTTTGTTCCTCAAGATGTG2720.1531247009283185No Hit
CTGAAAGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG2720.1531247009283185No Hit
CTGAAAGGGATCAGCTGGGATCACACCACTAGTCCCTGTCCTTTAACCCATG2700.15199878400972794No Hit
CTGAAAGGGGATAAAGATGGTGACGGCACCATCACAACCAAGGAACTGGGGA2670.15030990863184207No Hit
CTGAAAGGGAGGGATGTCCCTTTGAAGGCCTCTCTTGTTCTCCAGGCAGATA2650.14918399171325147No Hit
CTGAAAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT2650.14918399171325147No Hit
CTGAAAGGGAACTACTGTCAGCACTTACGATGTATGTATGGATGTGAAACAA2630.1480580747946609No Hit
CTGAAAGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAATG2620.1474951163353656No Hit
CTGAAAGGGGGTTCTTCCGGTCGCGAGCACCCCAGGGTGTAGAGGGCGGTCG2560.14411736557959387No Hit
CTGAAAGGGCAAGGAGATGCTGAAGCTCCTGGACTTTGGCAGTCTCTCTAAC2510.14130257328311746No Hit
CTGAAAGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGC2510.14130257328311746No Hit
CTGAAAGGGGGAGGGGAGGTGGGAGGCCCAGGGCTTTGCCTAGGGGTGAGCG2500.14073961482382216No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG2480.13961369790523157No Hit
CTGAAAGGGGGTATCTGGGGCGGGAACGTCTTTACTCTTTCCTGTAACTTAA2460.138487780986641No Hit
CTGAAAGGGCAAGTGTTACTGTCTCTCATTCCTCTGTCTCTGAGATCCCTTC2460.138487780986641No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT2450.1379248225273457No Hit
CTGAAAGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGT2440.13736186406805043No Hit
CTGAAAGGGAGTGCTACTTCTTTTACAGACTACACTTTATAGAACTGATTAG2440.13736186406805043No Hit
CTGAAAGGGAGGACCCATATGGTGGAGGAGAGAATTGCTGCAAGTTGTCCTG2390.134547071771574No Hit
CTGAAAGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGCGG2390.134547071771574No Hit
CTGAAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT2350.13229523793439282No Hit
CTGAAAGGGGGGAAAAGGACCAAGATGGGGGCCGCAAGCTGACGCCTCAGGG2350.13229523793439282No Hit
CTGAAAGGGGATCTTCTTCTGGCTCTACGGCGATTAAGAAGAAACAGCAAGA2350.13229523793439282No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC2330.13116932101580223No Hit
CTGAAAGGGCAAAACGCTTTGTGTCTGGCAGAGTGGCGTGCTTCATGATCAA2320.13060636255650696No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGCTC2320.13060636255650696No Hit
CTGAAAGGCTGGCTCCCCTTGTCCTGACTCGGTGTATGGATCTGTGGTCATT2280.1283545287193258No Hit
CTGAAAGGGGGTGCAAGCGCCCCGCCCGCTCAAATATGGGCTCCTGCGGGGC2260.12722861180073522No Hit
CTGAAAGGGGTGCAGCCCTTCCCCCATAGGGACTGCTGTCCCCTGACCCCCT2250.12666565334143995No Hit
CTGAAAGGGGGGGCCTCGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGCGC2240.12610269488214465No Hit
CTGAAAGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGTAC2230.12553973642284935No Hit
CTGAAAGGGAGCTGGGCTGGAGGAGAGCTTCCGGAAGTTTGCCATCCATGGC2230.12553973642284935No Hit
CTGAAAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC2200.1238508610449635No Hit
CTGAAAGGGGTGTGTGTGGGTGATTGGATGTGGGGGTGGGGCCCGTGGAGAG2200.1238508610449635No Hit
CTGAAAGGGGGAACTCTACCCTGGTAGGAAAGCACCAAAGCATGTGGGGATG2170.12216198566707762No Hit
CTGAAAGGGCTGTCTCGCTCGCTCCCATGGAGTCACCTAAGCCAGCACAAGG2170.12216198566707762No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG2160.12159902720778233No Hit
CTGAAAGGGGGCTCTCGGCGGTGCCGGCGGGAAGCGGAGGCCATGGAACAAC2160.12159902720778233No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTACTC2160.12159902720778233No Hit
CTGAAAGGGGGGACTCCGATGGGTCGAAGTCGTAGCCGCTCCCCGCGTAGGG2150.12103606874848705No Hit
CTGAAAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA2140.12047311028919175No Hit
CTGAAAGGGCTTTAAAATAGAAGGGGTTTCGTTTTTCTCTCTCTCTTGCTTC2120.11934719337060118No Hit
CTGAAAGGGCAGAGTGCAGTGCTCTGAAAGGGGCCGAAGCCGCGCCCTACAC2100.11822127645201061No Hit
CTGAAAGGGACTGGCTGACCTTCCACCTGGTCCGGTGATCCCTGGTCCTTAG2100.11822127645201061No Hit
CTGAAAGGGACCCAAGGGGGTGGGGCCTTGTGGGATCTCTGAGCCTGAAGGC2070.11653240107412474No Hit
CTGAAAGGGGGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAGC2060.11596944261482946No Hit
CTGAAAGGGACTGCCCACTGTGAAACAAAGCTTTGACTAGGTTGTTTTGTTT2050.11540648415553417No Hit
CTGAAAGGGACTCTGACTGGATTGAACGCTTAGCTTCAGCCTCATTCCTAGC2030.11428056723694359No Hit
CTGAAAGGGATTTCTCTAGATCTTAACATTTTCCCCAGCCTCCAAGTCCTCT2030.11428056723694359No Hit
CTGAAAGGGGGGCGTGCCCTGCACTCTCTGCTGCCCGCCGCCGACCCCTCCT2020.1137176087776483No Hit
CTGAAAGGGAGCGAGCTGGCCTGGCTGGACCTTGTTCCCTGGGGGAGGGGGC2020.1137176087776483No Hit
CTGAAAGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTGTC2000.11259169185905771No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT1990.11202873339976242No Hit
CTGAAAGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG1990.11202873339976242No Hit
CTGAAAGGGAGACAGTAGGCAGTCTGAAGTCCACACCTGACAATGCGTGCTC1950.10977689956258127No Hit
CTGAAAGGGATCTCTGGTGCTGGGGACCCCGCCAGGCCTCGCTTGCTGTGAC1940.10921394110328599No Hit
CTGAAAGGGAAGGGGACTCCCAGCTCAGCTGGGGCCCCCAGGGGTGGGAACT1940.10921394110328599No Hit
CTGAAAGGGGGCTGGGTGGTCGGGATGCTGGCCCCCCTCCTCATTTCCCTCA1940.10921394110328599No Hit
CTGAAAGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTT1930.1086509826439907No Hit
CTGAAAGGGGGGGATACTATACAGAGGGGATCTTCCTGTGCCTTAAGGCAGG1900.10696210726610483No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC1890.10639914880680955No Hit
CTGAAAGGGAAGCAGTGGGAAGCATTAGTATCAACGCAGAGTAACTTCAGTG1890.10639914880680955No Hit
CTGAAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC1860.1047102734289237No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCTCT1860.1047102734289237No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC1840.1035843565103331No Hit
CTGAAAGGGAAGCGACTGCGGGTGAGTTGGGAGGGAGAACGGGAAAACTTGT1840.1035843565103331No Hit
CTGAAAGGGGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACC1840.1035843565103331No Hit
CTGAAAGGGGGAAAGCATGGCTTAGAAGTAATTTATATGATTGAGCCCATTG1810.10189548113244723No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC1810.10189548113244723No Hit
CTGAAAGGGAACGACCTCCCTCACTCCCCAGTGCCAGGGAAGTAACCCCTTC1790.10076956421385666No Hit
CTGAAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC1780.10020660575456138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTTT551.8189894E-1246.00000434
TTAACCC551.8189894E-1246.00000443
ACCACTA551.8189894E-1246.00000425
TAGTCCC551.8189894E-1246.00000430
CACCACT551.8189894E-1246.00000424
TAATCAC253.4054174E-546.025
AGGTCAA206.296981E-446.029
TTGATTA253.4054174E-546.032
ACAACTT206.296981E-446.030
CAGGTTA206.296981E-446.022
TTCTATC301.8524643E-646.021
CTTAACA206.296981E-446.022
GTGTTAC253.4054174E-546.013
GAAATTC206.296981E-446.012
CAGAATA206.296981E-446.023
TATCACA301.8524643E-646.013
AACGTCT253.4054174E-546.025
ACTATTA206.296981E-446.039
TTAGGCT206.296981E-446.016
AACAACT206.296981E-446.038
Produced by FastQC (version 0.11.5)