FastQCFastQC Report
Fri 17 Jun 2016
SRR1527364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229819
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC18070.7862709349531588No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT15120.6579090501655651No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12970.5643571680322341No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12510.544341416506033No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9620.41859028191750897No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC7710.3354814005804568No Hit
CTGAAAGGGGGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGAGGCAGA7600.33069502521549565No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT7380.32112227448557346No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7350.3198168993860386No Hit
CTGAAAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7210.31372514892154263No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA6950.3024118980589072No Hit
CTGAAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA6710.2919688972626284No Hit
CTGAAAGGGGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGA6630.2884878969972022No Hit
CTGAAAGGGGGTGCTGGAGAGATGGCTCAGAGGTTAAGAGAACTGTCTGCTG6230.2710828956700708No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5980.2602047698406137No Hit
CTGAAAGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG5720.24889151897797834No Hit
CTGAAAGGGGGAGGAGCGTCAACTGCGGCACCATGGTAGCTCAACCGAAGAA5420.23583776798262981No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT5250.228440642418599No Hit
CTGAAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC4990.21712739155596358No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA4930.2145166413568939No Hit
CTGAAAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG4810.20929514095875448No Hit
CTGAAAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC4730.2058141406933282No Hit
CTGAAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA4700.20450876559379338No Hit
CTGAAAGGGGGCTGGCGCCCTTGCGAGGAGCCCTCTGCTGGTTCCACACTAC4680.20363851552743678No Hit
CTGAAAGGGCAGTGGGCAGTGATGGCATTACTCTGCACTCTAGCCACTTGCC4580.19928726519565396No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG4570.19885214016247568No Hit
CTGAAAGGGAAGCAGGCTACCTGGCCCGCAGGTCATGTGTGCAAGAGTAGGA4430.19276038969797973No Hit
CTGAAAGGGGGTGGTCTCCCTGCCTCAGCCAGGCTACTGCTGGGACACGCAC4410.19189013963162316No Hit
CTGAAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC4350.18927938943255346No Hit
CTGAAAGGGGAAAAAAGCCTCATGATGATCATGAAGGCAATGTAGATTATGG4320.1879740143330186No Hit
CTGAAAGGGATTGCGCAGGGGAGCCACGGAACTCACTCATGAGGACTACATG4280.18623351420030546No Hit
CTGAAAGGGGGAGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCGG4160.18101201380216606No Hit
CTGAAAGGGGCCGCCGCCGCCACCCCGCCCTTGCCTCCGGTTCACGCTAAAG4120.17927151366945293No Hit
CTGAAAGGGGAGTGGACCAGCGGGTCCAACACAGGGAAATCCAAGTCTGTGC4080.17753101353673978No Hit
CTGAAAGGGTGAGCACACAAAGGGCCAGAGTCAGAAAAGGAAGGGAACAAGT4060.1766607634703832No Hit
CTGAAAGGGGTCTATCACATAAAAGCAAAAGGATTTTGTTCCTCAAGATGTG4020.17492026333767008No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC3860.16795826280681753No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3830.1666528877072827No Hit
CTGAAAGGGGTAGCCACATGGCCCTGGACTGGATTATGAAGGAGCGTGAATC3810.16578263764092613No Hit
CTGAAAGGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATT3800.16534751260774783No Hit
CTGAAAGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGCTC3770.16404213750821298No Hit
CTGAAAGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG3770.16404213750821298No Hit
CTGAAAGGGGAGCCGGAGGGCAGCCATGGCGGCCGACAGCCGGGAGGAGAAA3640.1583855120768953No Hit
CTGAAAGGGCAAAACGCTTTGTGTCTGGCAGAGTGGCGTGCTTCATGATCAA3600.15664501194418218No Hit
CTGAAAGGGGGGGCCTCGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGCGC3550.15446938677829072No Hit
CTGAAAGGGAGTGCTACTTCTTTTACAGACTACACTTTATAGAACTGATTAG3540.15403426174511245No Hit
CTGAAAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT3500.15229376161239933No Hit
CTGAAAGGGTCCGCCCCAGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCAAA3490.15185863657922105No Hit
CTGAAAGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGT3470.15098838651286448No Hit
CTGAAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCA3460.15055326147968617No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3450.1501181364465079No Hit
CTGAAAGGGAACTACTGTCAGCACTTACGATGTATGTATGGATGTGAAACAA3420.14881276134697305No Hit
CTGAAAGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAATG3320.14446151101519022No Hit
CTGAAAGGGACCCAAGGGGGTGGGGCCTTGTGGGATCTCTGAGCCTGAAGGC3310.14402638598201195No Hit
CTGAAAGGGGGGAAAAGGACCAAGATGGGGGCCGCAAGCTGACGCCTCAGGG3270.1422858858492988No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT3250.14141563578294222No Hit
CTGAAAGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGCGG3200.13924001061705082No Hit
CTGAAAGGGGATAAAGATGGTGACGGCACCATCACAACCAAGGAACTGGGGA3190.13880488558387252No Hit
CTGAAAGGGAGGGATGTCCCTTTGAAGGCCTCTCTTGTTCTCCAGGCAGATA3170.13793463551751595No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG3160.13749951048433767No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC3130.13619413538480282No Hit
CTGAAAGGGGGTGCAAGCGCCCCGCCCGCTCAAATATGGGCTCCTGCGGGGC3110.13532388531844627No Hit
CTGAAAGGGATCAGCTGGGATCACACCACTAGTCCCTGTCCTTTAACCCATG3100.13488876028526797No Hit
CTGAAAGGGGAATGAAAAGTAATCAAGCACTTGGGAAGCAGAGGCAGGTGGA3100.13488876028526797No Hit
CTGAAAGGGGATCTTCTTCTGGCTCTACGGCGATTAAGAAGAAACAGCAAGA3100.13488876028526797No Hit
CTGAAAGGGGGGGATACTATACAGAGGGGATCTTCCTGTGCCTTAAGGCAGG3090.1344536352520897No Hit
CTGAAAGGGCAGAGTGCAGTGCTCTGAAAGGGGCCGAAGCCGCGCCCTACAC3060.13314826015255482No Hit
CTGAAAGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGTAC3050.13271313511937655No Hit
CTGAAAGGGCAAGGAGATGCTGAAGCTCCTGGACTTTGGCAGTCTCTCTAAC3040.13227801008619827No Hit
CTGAAAGGGAGGACCCATATGGTGGAGGAGAGAATTGCTGCAAGTTGTCCTG3020.1314077600198417No Hit
CTGAAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT3000.13053750995348515No Hit
CTGAAAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC3000.13053750995348515No Hit
CTGAAAGGGCTTTAAAATAGAAGGGGTTTCGTTTTTCTCTCTCTCTTGCTTC2990.13010238492030685No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTACTC2990.13010238492030685No Hit
CTGAAAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA2970.12923213485395027No Hit
CTGAAAGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGC2940.12792675975441542No Hit
CTGAAAGGGAATTTTTAAAGCTCACATAGATATTACTTGAGGAGTGAAACTG2930.12749163472123715No Hit
CTGAAAGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG2870.12488088452216745No Hit
CTGAAAGGGAGCGAGCTGGCCTGGCTGGACCTTGTTCCCTGGGGGAGGGGGC2820.12270525935627603No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC2820.12270525935627603No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGCTC2770.1205296341903846No Hit
CTGAAAGGGGTGTGTGTGGGTGATTGGATGTGGGGGTGGGGCCCGTGGAGAG2750.11965938412402805No Hit
CTGAAAGGGACTCTGACTGGATTGAACGCTTAGCTTCAGCCTCATTCCTAGC2740.11922425909084976No Hit
CTGAAAGGGGGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAGC2740.11922425909084976No Hit
CTGAAAGGGCAAGTGTTACTGTCTCTCATTCCTCTGTCTCTGAGATCCCTTC2740.11922425909084976No Hit
CTGAAAGGGGGTTCTTCCGGTCGCGAGCACCCCAGGGTGTAGAGGGCGGTCG2730.11878913405767148No Hit
CTGAAAGGGATTTCTCTAGATCTTAACATTTTCCCCAGCCTCCAAGTCCTCT2730.11878913405767148No Hit
CTGAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2720.11835400902449318No Hit
CTGAAAGGGACCACTTTCCGGAAGCTACAGGGGCTTGAGGTCATGAGAACCT2710.11791888399131491No Hit
CTGAAAGGGAACGTCACGTCCTTCCCTCCCACTCACCAGGGAACTTTTTTGA2690.11704863392495835No Hit
CTGAAAGGGGGTATCTGGGGCGGGAACGTCTTTACTCTTTCCTGTAACTTAA2680.11661350889178004No Hit
CTGAAAGGGGGAGGGGAGGTGGGAGGCCCAGGGCTTTGCCTAGGGGTGAGCG2680.11661350889178004No Hit
CTGAAAGGGGGAAAGCATGGCTTAGAAGTAATTTATATGATTGAGCCCATTG2620.11400275869271036No Hit
CTGAAAGGGGGGACTCCGATGGGTCGAAGTCGTAGCCGCTCCCCGCGTAGGG2610.11356763365953206No Hit
CTGAAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC2590.11269738359317549No Hit
CTGAAAGGGGTGCAGCCCTTCCCCCATAGGGACTGCTGTCCCCTGACCCCCT2550.11095688346046237No Hit
CTGAAAGGGACTGCCCACTGTGAAACAAAGCTTTGACTAGGTTGTTTTGTTT2540.11052175842728408No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCTCT2490.10834613326139265No Hit
CTGAAAGGGGGGGCTGTCCTGGACAAAGGTCGTGCCATGTTCTTAGTGTCCA2480.10791100822821438No Hit
CTGAAAGGGGGGTCACCCAAGCATGAAAGTAGGCCTCGGGGCCCACCCTGAC2470.10747588319503609No Hit
CTGAAAGGGGGAACTCTACCCTGGTAGGAAAGCACCAAAGCATGTGGGGATG2460.10704075816185782No Hit
CTGAAAGGGAGCTGGGCTGGAGGAGAGCTTCCGGAAGTTTGCCATCCATGGC2450.10660563312867952No Hit
CTGAAAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT2440.10617050809550124No Hit
CTGAAAGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTGTC2420.10530025802914468No Hit
CTGAAAGGGCTGGGAGTCCTCAGCTGGGGGATCCTTCACAGTGAGGACTGAC2420.10530025802914468No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG2400.1044300079627881No Hit
CTGAAAGGGGGGCGTGCCCTGCACTCTCTGCTGCCCGCCGCCGACCCCTCCT2380.10355975789643154No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT2370.10312463286325327No Hit
CTGAAAGGGGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACC2360.10268950783007497No Hit
CTGAAAGGGACTGGCTGACCTTCCACCTGGTCCGGTGATCCCTGGTCCTTAG2350.10225438279689669No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG2350.10225438279689669No Hit
CTGAAAGGGATGTGCAACTGTAGTTATTTGTAATCTAATAATTTTCATTAAT2350.10225438279689669No Hit
CTGAAAGGGGGAGGCGCATCAAGATGGCGGCCGAGACGGTGGAGCTCCACAA2340.10181925776371839No Hit
CTGAAAGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTT2330.10138413273054012No Hit
CTGAAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC2330.10138413273054012No Hit
CTGAAAGGGGCCCCTTCTCCCCGGCGGTTAGTGCTGAGAGTGCGGAGTGTGT2320.10094900769736183No Hit
CTGAAAGGGGGCTGGGTGGTCGGGATGCTGGCCCCCCTCCTCATTTCCCTCA2320.10094900769736183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG206.3008664E-446.023
TCGCCGT206.3008664E-446.032
ATTTAGG206.3008664E-446.034
CTAGATT253.4085686E-546.027
GAAATTA253.4085686E-546.033
ACAACCA253.4085686E-546.034
TTAGGTT206.3008664E-446.036
AGACTTC206.3008664E-446.019
GTGCACC405.5770215E-946.020
GAGTGTA206.3008664E-446.030
CGGGTGT206.3008664E-446.015
AACGTCA253.4085686E-546.010
AACGTAT206.3008664E-446.045
AACCGTG253.4085686E-546.032
AAACTCA253.4085686E-546.027
AGCCGTT253.4085686E-546.028
TAGAGCG253.4085686E-546.011
AGGTAAT206.3008664E-446.046
TCGTTAG253.4085686E-546.013
GTACCGC253.4085686E-546.027
Produced by FastQC (version 0.11.5)