FastQCFastQC Report
Fri 17 Jun 2016
SRR1527363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792707
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC69390.8753549546049171No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49230.6210365242138647No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC39510.49841870956103584No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC36240.45716765463153475No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC25030.31575348773254175No Hit
GTATACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA23990.3026338861647494No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT23680.2987232356974267No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG22840.2881266344311328No Hit
GTATACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA21350.2693302821849687No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC20240.25532763051165186No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG19750.24914627977298043No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19730.24889397974283056No Hit
GTATACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA17410.21962717624544756No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA17220.21723032595902395No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA15930.2009569740143584No Hit
GTATACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA15140.19099112282343916No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15050.18985577268776482No Hit
GTATACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT15000.1892250226123902No Hit
GTATACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG14840.18720662237119137No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12590.15882286897933284No Hit
GTATACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12290.15503836852708505No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC12080.15238921821051157No Hit
GTATACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC12020.15163231812006203No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG11640.1468386175472148No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG11590.14620786747184017No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT11450.14444176726079117No Hit
GTATACGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG10510.13258366584374806No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC10290.12980836551209968No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG10250.12930376545179997No Hit
GTATACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG9640.12160861453222944No Hit
GTATACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG9560.12059941441163002No Hit
GTATACGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTC8970.11315656352220935No Hit
GTATACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT8820.11126431329608544No Hit
GTATACGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA8780.11075971323578573No Hit
GTATACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA8300.10470451251218924No Hit
GTATACGGGACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATA8130.10255996225591549No Hit
GTATACGGGACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTT7970.10054156201471666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT254.4415858E-544.019
AACGTTG351.4453326E-744.028
AACGTAC351.4453326E-744.013
AGCGTAA207.855001E-444.040
GTATTAC302.5267182E-644.037
GGTAACG254.4415858E-544.022
TAGCGTA302.5267182E-644.021
GGCGTAT302.5267182E-644.017
CGATTTC207.855001E-444.019
CACGATT254.4415858E-544.015
GTAATCT207.855001E-444.043
TATTGGT207.855001E-444.020
ATTCACC207.855001E-444.011
CGGATTA207.855001E-444.06
ACGGATA254.4415858E-544.05
TCGCTAA207.855001E-444.028
ACGGACT207.855001E-444.05
GTATCGT207.855001E-444.010
GCGATAT551.8189894E-1244.011
CGTACCC351.4453326E-744.015
Produced by FastQC (version 0.11.5)