##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792707
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138641389567645	31.0	31.0	33.0	28.0	34.0
2	30.51312527831847	31.0	31.0	33.0	28.0	34.0
3	31.727055519883134	33.0	31.0	34.0	30.0	34.0
4	35.917960860696326	37.0	35.0	37.0	35.0	37.0
5	35.52120518678402	37.0	35.0	37.0	33.0	37.0
6	36.07886394342424	37.0	35.0	37.0	35.0	37.0
7	36.27964809191795	37.0	37.0	37.0	35.0	37.0
8	36.49909865814229	37.0	37.0	37.0	35.0	37.0
9	38.48450940889887	39.0	39.0	39.0	38.0	39.0
10	37.21699316393068	39.0	37.0	39.0	34.0	39.0
11	37.09714686510905	39.0	37.0	39.0	34.0	39.0
12	36.922067043687015	38.0	37.0	39.0	34.0	39.0
13	37.02392939635956	39.0	37.0	39.0	34.0	39.0
14	37.86517086388792	40.0	38.0	40.0	34.0	40.0
15	37.90723054041405	40.0	38.0	40.0	34.0	40.0
16	38.02766217530563	40.0	38.0	40.0	34.0	40.0
17	37.91860044127275	40.0	38.0	40.0	34.0	40.0
18	37.75508226873233	40.0	37.0	40.0	34.0	40.0
19	37.706962345482	39.0	37.0	40.0	34.0	40.0
20	37.50688085257226	39.0	37.0	40.0	33.0	40.0
21	37.42227708346211	39.0	36.0	40.0	33.0	40.0
22	37.20096580451541	38.0	36.0	40.0	33.0	40.0
23	36.986132328842814	38.0	36.0	40.0	33.0	40.0
24	36.84118848452202	38.0	35.0	40.0	32.0	40.0
25	36.59717146436199	38.0	35.0	40.0	32.0	40.0
26	36.123386068244635	38.0	35.0	40.0	31.0	40.0
27	35.88318887054107	38.0	35.0	39.0	31.0	40.0
28	35.67931656967833	37.0	34.0	39.0	30.0	40.0
29	35.29587098385658	37.0	34.0	38.0	30.0	40.0
30	34.981261676760774	36.0	34.0	38.0	30.0	40.0
31	34.87488819954914	36.0	33.0	38.0	29.0	40.0
32	34.78785982714925	36.0	33.0	38.0	29.0	40.0
33	34.17237138059838	36.0	33.0	38.0	28.0	39.0
34	33.691093935085725	35.0	32.0	38.0	27.0	39.0
35	33.58646385108243	35.0	32.0	38.0	27.0	39.0
36	33.13509783564419	34.0	31.0	37.0	26.0	38.0
37	32.95107019365289	34.0	31.0	37.0	26.0	38.0
38	32.46634759122854	34.0	31.0	37.0	25.0	38.0
39	31.678635359596925	33.0	30.0	36.0	23.0	38.0
40	31.253832752833013	33.0	29.0	36.0	23.0	38.0
41	30.984439395640507	33.0	29.0	35.0	23.0	38.0
42	30.7023288554283	32.0	29.0	35.0	22.0	38.0
43	30.14925817483635	32.0	28.0	35.0	21.0	37.0
44	29.64517028359785	31.0	27.0	34.0	21.0	37.0
45	29.095231907880212	31.0	26.0	34.0	20.0	36.0
46	29.003271069890893	31.0	26.0	34.0	20.0	36.0
47	28.496251452302047	30.0	26.0	33.0	19.0	36.0
48	27.953382523429212	30.0	24.0	33.0	18.0	35.0
49	27.272288500038474	29.0	24.0	32.0	17.0	35.0
50	30.982335213389057	33.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	14.0
13	42.0
14	74.0
15	140.0
16	219.0
17	374.0
18	500.0
19	736.0
20	1102.0
21	1495.0
22	1985.0
23	2862.0
24	4008.0
25	5296.0
26	7015.0
27	9161.0
28	12386.0
29	16901.0
30	23129.0
31	32015.0
32	49730.0
33	76267.0
34	110869.0
35	200238.0
36	203732.0
37	31868.0
38	542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.54787834597147	0.29771403557682724	0.04781085571339726	0.10659676273831314
8	99.43282953222314	0.39106504673227305	0.10924591305488661	0.0668595079897112
9	98.0624619184642	1.1192029337447507	0.3930834469734719	0.4252517008175783
10	58.28446071499306	30.470653091243044	4.390020524607452	6.854865669156447
11	35.59612820373732	24.5158677796462	20.413090839364358	19.474913177252123
12	28.097897457698746	18.321145139376842	26.614877880477906	26.966079522446503
13	24.930775179227634	17.114898695230394	29.889984571853155	28.064341553688816
14	25.52077879973307	17.62694160641952	29.70479634972316	27.14748324412425
15	26.34731369850399	17.524886244223907	27.20046625045572	28.927333806816392
16	29.56237298270357	18.416136100728263	25.736621475526267	26.2848694410419
17	30.53890024939858	18.534212514838398	23.247050928022585	27.67983630774044
18	29.26894804763929	20.112601503455878	23.576807067428447	27.041643381476383
19	30.74351557385011	20.713454025257754	22.331075668563543	26.21195473232859
20	30.43255578669042	20.83241348947341	22.399953576794452	26.33507714704172
21	31.143537271652704	19.39543866775492	25.16402655710117	24.296997503491202
22	31.931470265810695	17.68421371326354	24.08884997861757	26.295466042308192
23	28.890119552369285	19.661110599502717	24.046463573552394	27.4023062745756
24	29.32849085475466	18.937766413063088	23.872124252718848	27.861618479463406
25	27.158205995405616	19.650135548191198	25.10372684989536	28.087931606507826
26	27.152024644666945	19.827502469386545	23.268496430585323	29.751976455361184
27	29.19843018921241	18.398853548663	22.39124922575428	30.01146703637031
28	30.253296615268944	20.827367488870415	21.299799295326018	27.619536600534623
29	29.089058126142447	20.406404888565387	22.353151921201654	28.15138506409052
30	29.357631508236963	20.03728994445615	22.87478223353648	27.73029631377041
31	31.295926489863213	20.750163679644558	21.57266177793308	26.38124805255914
32	32.663392653275416	19.7326376580502	21.631321534942924	25.972648153731452
33	32.96526963934972	19.598161741980327	22.464794684542966	24.971773934126983
34	28.61120186903862	22.735008016833458	25.180173759030765	23.473616355097153
35	29.79537206054696	22.945047791933213	24.267604549978746	22.991975597541085
36	29.506362376010305	23.061862705892594	21.74901949900783	25.682755419089272
37	30.196529108485226	23.381652994107533	20.931441251307227	25.49037664610001
38	32.14037469077478	22.467569984874615	21.286427393728076	24.10562793062254
39	31.21733503047154	21.48170761706406	21.13429047554771	26.166666876916693
40	29.017026467534663	21.321749397949052	23.00610439922948	26.65511973528681
41	27.59796494795681	21.93357697106245	22.871754633174678	27.596703447806064
42	27.460209131494988	18.859931853761857	23.58752981870981	30.09232919603334
43	26.61878853094523	18.997687670223677	25.75630087787795	28.627222920953137
44	27.147230944094098	19.398844718161946	24.712409503132935	28.74151483461102
45	26.98954342525044	20.233957817957958	24.559641834877198	28.216856921914403
46	27.73559461440356	21.15472677798985	23.36853339253974	27.741145215066854
47	27.826044175212278	20.91529404937764	24.62915049318348	26.6295112822266
48	28.625961420802387	21.25816979035129	25.3314276271056	24.78444116174072
49	28.460831051069313	20.451188143916983	24.181948689742867	26.90603211527084
50	26.505253517377795	21.225496936446884	23.78104394183475	28.48820560434057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	11.5
8	23.0
9	111.0
10	199.0
11	305.0
12	411.0
13	569.5
14	728.0
15	628.5
16	529.0
17	608.0
18	687.0
19	805.5
20	924.0
21	1275.0
22	1626.0
23	1613.0
24	1600.0
25	2269.5
26	2939.0
27	3297.5
28	3656.0
29	4090.5
30	4525.0
31	5508.0
32	6491.0
33	7404.5
34	8318.0
35	8603.0
36	8888.0
37	11396.0
38	13904.0
39	13679.0
40	13454.0
41	15778.5
42	18103.0
43	18686.5
44	19270.0
45	21730.0
46	24190.0
47	28649.5
48	33109.0
49	36022.5
50	38936.0
51	42592.0
52	46248.0
53	49903.0
54	53558.0
55	64734.0
56	75910.0
57	76573.0
58	77236.0
59	78349.0
60	79462.0
61	77306.0
62	75150.0
63	67581.0
64	60012.0
65	49926.5
66	39841.0
67	32522.5
68	25204.0
69	21932.5
70	18661.0
71	16352.0
72	14043.0
73	15018.0
74	15993.0
75	10931.0
76	5869.0
77	3743.0
78	1617.0
79	1289.0
80	961.0
81	655.5
82	350.0
83	203.5
84	57.0
85	39.5
86	22.0
87	11.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	792707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.082774593891564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97774088806757	8.69690818928053
2	9.282634342928137	2.2431995680623484
3	3.1321452062517614	1.1353501356743412
4	1.457491569309153	0.7044216841783912
5	0.7370981718712479	0.44530955321449167
6	0.5669182823315689	0.41099674911411155
7	0.40300268320439336	0.3408573407324522
8	0.3236550046460154	0.31285203738581846
9	0.289201407377246	0.31449198758179253
>10	8.132093003831658	27.453901630741246
>50	2.44724945448471	20.021647342586856
>100	1.169334210333991	26.254593437423917
>500	0.05115837170211211	4.14339724513597
>1k	0.029233355258349778	6.646718144282818
>5k	0.0010440484020839207	0.8753549546049171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6939	0.8753549546049171	No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4923	0.6210365242138647	No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3951	0.49841870956103584	No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3624	0.45716765463153475	No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2503	0.31575348773254175	No Hit
GTATACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2399	0.3026338861647494	No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2368	0.2987232356974267	No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2284	0.2881266344311328	No Hit
GTATACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	2135	0.2693302821849687	No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2024	0.25532763051165186	No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1975	0.24914627977298043	No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1973	0.24889397974283056	No Hit
GTATACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1741	0.21962717624544756	No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1722	0.21723032595902395	No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1593	0.2009569740143584	No Hit
GTATACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1514	0.19099112282343916	No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1505	0.18985577268776482	No Hit
GTATACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	1500	0.1892250226123902	No Hit
GTATACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1484	0.18720662237119137	No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1259	0.15882286897933284	No Hit
GTATACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1229	0.15503836852708505	No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1208	0.15238921821051157	No Hit
GTATACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	1202	0.15163231812006203	No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1164	0.1468386175472148	No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1159	0.14620786747184017	No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1145	0.14444176726079117	No Hit
GTATACGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG	1051	0.13258366584374806	No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1029	0.12980836551209968	No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1025	0.12930376545179997	No Hit
GTATACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	964	0.12160861453222944	No Hit
GTATACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	956	0.12059941441163002	No Hit
GTATACGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTC	897	0.11315656352220935	No Hit
GTATACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	882	0.11126431329608544	No Hit
GTATACGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA	878	0.11075971323578573	No Hit
GTATACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA	830	0.10470451251218924	No Hit
GTATACGGGACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATA	813	0.10255996225591549	No Hit
GTATACGGGACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTT	797	0.10054156201471666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001261500150749268	0.0	0.0	0.0	0.0
8	0.0015138001808991217	0.0	0.0	0.0	0.0
9	0.0016399501959740484	0.0	0.0	0.0	0.0
10	0.007190550859270828	0.0	0.0	0.0	0.0
11	0.012867301537642534	0.0	0.0	0.0	0.0
12	0.01778715212556468	0.0	0.0	0.0	0.0
13	0.02245470268333697	0.0	0.0	0.0	0.0
14	0.029392953512457944	0.0	0.0	0.0	0.0
15	0.03557430425112936	0.0	0.0	0.0	0.0
16	0.042260255050100476	0.0	0.0	0.0	0.0
17	0.048189305758622035	0.0	0.0	0.0	0.0
18	0.05399220645206867	0.0	0.0	0.0	0.0
19	0.0611827573113395	0.0	0.0	0.0	0.0
20	0.0682471581555354	0.0	0.0	0.0	0.0
21	0.0720316586077832	0.0	0.0	0.0	0.0
22	0.0753115589997313	0.0	0.0	0.0	0.0
23	0.08098830967810301	0.0	0.0	0.0	0.0
24	0.08552971022080037	0.0	0.0	0.0	0.0
25	0.09044956080872252	0.0	0.0	0.0	0.0
26	0.09675706156246885	0.0	0.0	0.0	0.0
27	0.10558756261771374	0.0	0.0	0.0	0.0
28	0.1158057138387828	0.0	0.0	0.0	0.0
29	0.12085171444177988	0.0	0.0	0.0	0.0
30	0.12778996527090086	0.0	0.0	0.0	0.0
31	0.1364943163110708	0.0	0.0	0.0	0.0
32	0.14532481736631567	0.0	0.0	0.0	0.0
33	0.15125386807483723	0.0	0.0	0.0	0.0
34	0.15945361905470748	0.0	0.0	0.0	0.0
35	0.16828412010995236	0.0	0.0	0.0	0.0
36	0.1778715212556468	0.0	0.0	0.0	0.0
37	0.18569282219029226	0.0	0.0	0.0	0.0
38	0.19427102321538728	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTT	25	4.4415858E-5	44.0	19
AACGTTG	35	1.4453326E-7	44.0	28
AACGTAC	35	1.4453326E-7	44.0	13
AGCGTAA	20	7.855001E-4	44.0	40
GTATTAC	30	2.5267182E-6	44.0	37
GGTAACG	25	4.4415858E-5	44.0	22
TAGCGTA	30	2.5267182E-6	44.0	21
GGCGTAT	30	2.5267182E-6	44.0	17
CGATTTC	20	7.855001E-4	44.0	19
CACGATT	25	4.4415858E-5	44.0	15
GTAATCT	20	7.855001E-4	44.0	43
TATTGGT	20	7.855001E-4	44.0	20
ATTCACC	20	7.855001E-4	44.0	11
CGGATTA	20	7.855001E-4	44.0	6
ACGGATA	25	4.4415858E-5	44.0	5
TCGCTAA	20	7.855001E-4	44.0	28
ACGGACT	20	7.855001E-4	44.0	5
GTATCGT	20	7.855001E-4	44.0	10
GCGATAT	55	1.8189894E-12	44.0	11
CGTACCC	35	1.4453326E-7	44.0	15
>>END_MODULE