FastQCFastQC Report
Fri 17 Jun 2016
SRR1527362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703665
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC58510.8315036274363511No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44100.6267186800537188No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC37020.5261026198546184No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32280.45874102022979685No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC24020.3413556166641797No Hit
GTATACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG21490.3054010075817328No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20700.2941740743109292No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA20230.28749475958019793No Hit
GTATACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA20050.2849367241514073No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA19910.28294714104012564No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18960.26944639849928587No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT17260.24528717500515157No Hit
GTATACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16140.22937051011489842No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC15070.21416441062153155No Hit
GTATACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT14660.20833777436706388No Hit
GTATACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG13630.19370012719120605No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT13610.19341590103245154No Hit
GTATACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13010.18488911626981588No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC11740.16684075518890382No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC11480.16314581512509505No Hit
GTATACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT10860.15433480420370488No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC10850.15419269112432762No Hit
GTATACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT10830.15390846496557312No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10360.14722915023484187No Hit
GTATACGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAG10260.14580801944106928No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10030.14253941861539227No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG9860.14012349626597884No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9400.13358629461462485No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9270.1317388245827205No Hit
GTATACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9020.12818599759828894No Hit
GTATACGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC9020.12818599759828894No Hit
GTATACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG8970.12747543220140264No Hit
GTATACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA8830.125485849090121No Hit
GTATACGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG8170.11610638585122181No Hit
GTATACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA7880.11198510654928126No Hit
GTATACGGGATGACAAAAAACAACAACAAGAAATCCTGGCGGCGAAACCCTG7710.10956918419986784No Hit
GTATACGGGACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATATT7640.108574392644227No Hit
GTATACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT7630.10843227956484976No Hit
GTATACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7420.10544790489792728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC206.309776E-446.00000420
ATCCGTT206.309776E-446.00000438
ATCGTAC206.309776E-446.00000445
TAATTCC206.309776E-446.00000414
TATTATC206.309776E-446.00000444
GTATAGC206.309776E-446.0000049
TATAGAC206.309776E-446.00000414
TCATATC206.309776E-446.00000419
TCAACCG405.6061253E-946.00000422
AACGTAG253.4157965E-546.027
AACGTAC253.4157965E-546.013
CGTGCAA900.046.013
TAAGTCG253.4157965E-546.041
AAACGAC253.4157965E-546.022
AATGCGT501.6370905E-1146.010
ACGATAT253.4157965E-546.029
TACGCGG253.4157965E-546.04
CGTACTA253.4157965E-546.034
TCGTACT253.4157965E-546.033
ATGCGTC551.8189894E-1246.011
Produced by FastQC (version 0.11.5)