FastQCFastQC Report
Fri 17 Jun 2016
SRR1527361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786886
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC63820.8110450560818213No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG51390.6530806241310685No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42370.538451567317248No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT37120.47173288125598883No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC27360.34769966678781933No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23400.29737471501589813No Hit
GTATACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG22400.28466639386137255No Hit
GTATACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA22230.2825059792651032No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA21310.2708143238029397No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA21190.2692893252643966No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20020.25442058951360175No Hit
GTATACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG18560.23586644062799442No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT18530.23548519099335863No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17750.22557270049282868No Hit
GTATACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT16500.20968729904967173No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT16110.2047310537994068No Hit
GTATACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15040.19113315016406443No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14680.18655815454843522No Hit
GTATACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG14480.18401649031753012No Hit
GTATACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12710.16152276187401987No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC12560.15961651370084104No Hit
GTATACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT12140.1542790188159403No Hit
GTATACGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAG11510.14627277648858922No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG11420.1451290275846819No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG11330.1439852786807746No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC11230.14271444656532203No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11210.14246028014223153No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10910.13864778379587386No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10210.12975195898770597No Hit
GTATACGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC9870.12543112979516727No Hit
GTATACGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG9660.12276238235271691No Hit
GTATACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA9650.12263529914117166No Hit
GTATACGGGACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATATT9540.12123738381417384No Hit
GTATACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG9360.11894988600635924No Hit
GTATACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA9070.11526447287154684No Hit
GTATACGGGACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCT9040.11488322323691108No Hit
GTATACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC8840.11234155900600597No Hit
GTATACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8670.11018114440973661No Hit
GTATACGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC8450.10738531375574098No Hit
GTATACGGGATGACAAAAAACAACAACAAGAAATCCTGGCGGCGAAACCCTG8240.10471656631329061No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT8030.10204781887084025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG351.01901605E-746.00000427
AACGTAC206.310234E-446.00000413
GTCGAAA206.310234E-446.00000419
CCGATTT351.01901605E-746.00000437
TTAGCAC206.310234E-446.00000439
ACGCACA800.046.00000435
ATTACCC351.01901605E-746.00000445
CCGGATA206.310234E-446.00000417
GAATCGT405.6079443E-946.00000412
TGTGCTA351.01901605E-746.00000427
CATTAGG351.01901605E-746.00000420
TATTGCG206.310234E-446.00000431
AAACGAC206.310234E-446.00000422
AACGCAT206.310234E-446.00000422
CATACGT206.310234E-446.00000414
TCCGTAA206.310234E-446.00000442
CGATGCA206.310234E-446.00000434
ACGTAGG351.01901605E-746.00000428
TCATACG206.310234E-446.00000413
CGCACAT206.310234E-446.00000446
Produced by FastQC (version 0.11.5)