##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	786886
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.594823138294494	31.0	31.0	33.0	27.0	34.0
2	30.325283459103353	31.0	31.0	33.0	28.0	34.0
3	31.534209529715866	31.0	31.0	34.0	30.0	34.0
4	35.81385359505697	37.0	35.0	37.0	35.0	37.0
5	35.50755382609425	37.0	35.0	37.0	33.0	37.0
6	36.063725876429366	37.0	35.0	37.0	35.0	37.0
7	36.272440988910716	37.0	36.0	37.0	35.0	37.0
8	36.45829764413143	37.0	37.0	37.0	35.0	37.0
9	38.54667512193634	39.0	39.0	39.0	37.0	39.0
10	37.16733300630587	39.0	37.0	39.0	34.0	39.0
11	37.11735117920512	39.0	37.0	39.0	34.0	39.0
12	36.95147581733568	39.0	37.0	39.0	34.0	39.0
13	36.97156386058463	39.0	37.0	39.0	33.0	39.0
14	37.769314741906705	40.0	37.0	40.0	33.0	40.0
15	37.85430163962759	40.0	38.0	40.0	33.0	40.0
16	37.89525547538017	40.0	37.0	40.0	33.0	40.0
17	37.874263870497124	40.0	38.0	40.0	33.0	40.0
18	37.76980655393539	40.0	37.0	40.0	33.0	40.0
19	37.621023884018776	40.0	37.0	40.0	33.0	40.0
20	37.49268127784711	39.0	37.0	40.0	33.0	40.0
21	37.52269197825352	39.0	37.0	40.0	33.0	40.0
22	37.51324334147513	39.0	37.0	40.0	33.0	40.0
23	37.42552415470602	39.0	36.0	40.0	33.0	40.0
24	37.41750901655386	39.0	36.0	40.0	33.0	40.0
25	37.35346670292774	39.0	36.0	40.0	33.0	40.0
26	37.30174383582882	39.0	36.0	40.0	33.0	40.0
27	37.16736223544452	39.0	36.0	40.0	33.0	40.0
28	36.95404798153735	39.0	35.0	40.0	32.0	40.0
29	36.866593127848255	39.0	35.0	40.0	32.0	40.0
30	36.75834746075035	39.0	35.0	40.0	31.0	40.0
31	36.713854357556244	38.0	35.0	40.0	31.0	40.0
32	36.6212971129236	38.0	35.0	40.0	31.0	40.0
33	36.422141199614686	38.0	35.0	40.0	31.0	40.0
34	36.048342453671815	38.0	35.0	40.0	30.0	40.0
35	35.99343233962734	38.0	35.0	40.0	30.0	40.0
36	35.86580012860821	38.0	34.0	40.0	30.0	40.0
37	35.815233718734355	38.0	34.0	40.0	30.0	40.0
38	35.7191143316821	38.0	34.0	40.0	30.0	40.0
39	35.5124681338847	37.0	34.0	40.0	29.0	40.0
40	35.70681521846875	38.0	34.0	40.0	30.0	40.0
41	35.8349671998231	38.0	34.0	40.0	30.0	40.0
42	35.89703972367027	37.0	35.0	40.0	31.0	40.0
43	35.85169389212669	37.0	35.0	40.0	31.0	40.0
44	35.6882991945466	37.0	34.0	40.0	30.0	40.0
45	35.59834080159006	37.0	34.0	40.0	30.0	40.0
46	35.28121862633215	36.0	34.0	40.0	30.0	40.0
47	35.189534443362824	36.0	34.0	40.0	30.0	40.0
48	35.061518441044825	36.0	34.0	39.0	30.0	40.0
49	34.88885048151829	35.0	34.0	39.0	29.0	40.0
50	34.764100517737006	35.0	34.0	39.0	29.0	40.0
51	34.64784733747963	35.0	34.0	39.0	29.0	40.0
52	34.18282063729689	35.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	11.0
14	21.0
15	42.0
16	59.0
17	104.0
18	195.0
19	313.0
20	466.0
21	785.0
22	1103.0
23	1687.0
24	2346.0
25	3191.0
26	4305.0
27	5764.0
28	7676.0
29	10300.0
30	13807.0
31	18350.0
32	25687.0
33	37887.0
34	62181.0
35	71135.0
36	115051.0
37	168984.0
38	212644.0
39	22786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.56918791286158	0.3110997018627857	0.049562452502649686	0.0701499327729811
8	99.4546859392593	0.3775642215009544	0.11386655754454901	0.05388328169518838
9	98.07913725749347	1.111215601751715	0.3823933835396741	0.42725375721514935
10	58.32356910657961	30.345437585622314	4.31816044509624	7.01283286270184
11	35.40347648833503	24.531761907061505	20.37766588807019	19.68709571653327
12	27.84748997948877	18.452990649217295	26.651001542790187	27.048517828503748
13	24.908436546081646	17.072104472566547	29.89480051748284	28.124658463868972
14	25.408508983512224	17.592001890998187	29.657536161527847	27.34195296396174
15	26.437247580971068	17.47305200499183	27.241430143629447	28.848270270407657
16	29.516600879924155	18.33683659386493	25.805898186014236	26.340664340196675
17	30.45943122637841	18.428336506177516	23.230556904049635	27.881675363394443
18	29.1699178788287	20.102911984709348	23.52056079279591	27.206609343666045
19	30.51674575478532	20.593199014850942	22.37528689035006	26.514768340013674
20	30.56440195911479	20.749765531474697	22.32750360280905	26.358328906601464
21	31.241120060593275	19.383493924151658	25.117361345862044	24.258024669393023
22	31.980108935728936	17.723660098159073	24.062062357190243	26.23416860892175
23	28.881311905409422	19.596231220278415	23.978060354358828	27.544396519953334
24	29.288867764835057	18.82331112766017	23.970054112031473	27.917766995473297
25	27.226688491090194	19.600424966259407	25.014932277356568	28.157954265293828
26	27.000099124905002	19.59902705093241	23.287363099610364	30.113510724552224
27	29.300559420297223	18.40063236606065	22.3234369400396	29.97537127360253
28	30.403514613298494	20.758407189859778	21.262165040425167	27.575913156416558
29	29.061007566534414	20.357713823857583	22.365374399849532	28.215904209758463
30	29.399557242090978	19.988155844683984	22.610263748497243	28.002023164727802
31	31.31470124007798	20.726255137338825	21.60236679773182	26.356676824851377
32	32.844783107082854	19.53790002617914	21.54263768830555	26.074679178432454
33	32.860287258891375	19.513754215985543	22.465007637701014	25.160950887422068
34	28.768970346403417	22.676347018500774	25.011119781010212	23.543562854085597
35	29.819440173036497	22.84066561102879	24.15483310161828	23.185061114316433
36	29.498173814250094	22.995071713056277	21.61329595392471	25.89345851876892
37	30.30731262215874	23.373779683461137	20.92628411231106	25.392623582069067
38	32.30531487407325	22.278449483152578	21.266485869617707	24.14974977315647
39	31.157118057761863	21.452154441685327	21.094288117973885	26.296439382578924
40	29.046265913995168	21.341846214064045	22.916915537955944	26.694972333984847
41	27.5198694601251	21.916389413460145	23.05200499182855	27.511736134586208
42	27.656865162170885	18.699404996403544	23.701273119613262	29.94245672181231
43	26.645536964693743	18.864867337835467	25.8097106823606	28.679885015110195
44	27.341825880750196	19.204942011930573	24.633555559509254	28.819676547809976
45	26.977351230038405	20.110409894190518	24.580434777083337	28.33180409868774
46	27.787887953274044	21.14956931499607	23.26143812445513	27.801104607274752
47	27.702488035115636	20.8716383313466	24.797111652768	26.628761980769767
48	28.761853686556883	21.04383608299042	25.39389441418452	24.800415816268178
49	28.423558177423413	20.206866051753366	24.36807873059122	27.001497040232003
50	26.339393508081223	21.367771189219276	23.800016774983924	28.49281852771558
51	25.88939185599947	22.20906204964887	24.44585365605691	27.455692438294747
52	27.666269319825236	20.958817414466644	23.56224408618275	27.81266917952537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	2.0
6	0.0
7	19.0
8	38.0
9	107.5
10	177.0
11	279.0
12	381.0
13	578.0
14	648.0
15	521.0
16	645.5
17	770.0
18	846.5
19	923.0
20	872.0
21	821.0
22	1148.5
23	1476.0
24	1809.5
25	2143.0
26	2464.5
27	2786.0
28	3338.0
29	3890.0
30	4259.5
31	4629.0
32	4942.0
33	5255.0
34	7462.0
35	9669.0
36	10440.0
37	11211.0
38	11387.5
39	12678.0
40	13792.0
41	16011.5
42	18231.0
43	19123.5
44	20016.0
45	21352.5
46	22689.0
47	27791.5
48	32894.0
49	35241.5
50	37589.0
51	40985.5
52	44382.0
53	46784.0
54	49186.0
55	55978.5
56	62771.0
57	70951.0
58	79131.0
59	78259.0
60	77387.0
61	74742.0
62	72097.0
63	65897.5
64	53275.0
65	46852.0
66	36210.0
67	25568.0
68	23227.5
69	20887.0
70	18768.0
71	16649.0
72	16987.0
73	17325.0
74	12776.0
75	8227.0
76	5244.0
77	2261.0
78	1611.5
79	962.0
80	1004.0
81	1046.0
82	580.5
83	115.0
84	89.0
85	63.0
86	47.0
87	31.0
88	17.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	786886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.961036287340225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.89441453394912	6.762987268803868
2	9.847924268326912	1.9619106198356562
3	3.5594906993952695	1.0636864806337893
4	1.621545763057845	0.6460910474960795
5	0.9619555510193667	0.4791037075256136
6	0.6047306779617769	0.36142465363470694
7	0.43504886325942177	0.3033476259585251
8	0.362328085529841	0.2887330566308207
9	0.3546732668214641	0.31796219528622954
>10	9.641244163200735	26.97366581690359
>50	3.141027276670664	21.346421209679676
>100	1.4748284044806206	27.52724028639473
>500	0.06379015590314104	4.348914582290192
>1k	0.034446684187696155	6.154385768713638
>5k	0.0025516062361256413	1.46412568021289
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6382	0.8110450560818213	No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5139	0.6530806241310685	No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4237	0.538451567317248	No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3712	0.47173288125598883	No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2736	0.34769966678781933	No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2340	0.29737471501589813	No Hit
GTATACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2240	0.28466639386137255	No Hit
GTATACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2223	0.2825059792651032	No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2131	0.2708143238029397	No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2119	0.2692893252643966	No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2002	0.25442058951360175	No Hit
GTATACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1856	0.23586644062799442	No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1853	0.23548519099335863	No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1775	0.22557270049282868	No Hit
GTATACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	1650	0.20968729904967173	No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1611	0.2047310537994068	No Hit
GTATACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1504	0.19113315016406443	No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1468	0.18655815454843522	No Hit
GTATACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1448	0.18401649031753012	No Hit
GTATACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1271	0.16152276187401987	No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1256	0.15961651370084104	No Hit
GTATACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1214	0.1542790188159403	No Hit
GTATACGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAG	1151	0.14627277648858922	No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1142	0.1451290275846819	No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1133	0.1439852786807746	No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1123	0.14271444656532203	No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1121	0.14246028014223153	No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1091	0.13864778379587386	No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1021	0.12975195898770597	No Hit
GTATACGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC	987	0.12543112979516727	No Hit
GTATACGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG	966	0.12276238235271691	No Hit
GTATACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	965	0.12263529914117166	No Hit
GTATACGGGACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATATT	954	0.12123738381417384	No Hit
GTATACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	936	0.11894988600635924	No Hit
GTATACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	907	0.11526447287154684	No Hit
GTATACGGGACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCT	904	0.11488322323691108	No Hit
GTATACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	884	0.11234155900600597	No Hit
GTATACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	867	0.11018114440973661	No Hit
GTATACGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	845	0.10738531375574098	No Hit
GTATACGGGATGACAAAAAACAACAACAAGAAATCCTGGCGGCGAAACCCTG	824	0.10471656631329061	No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	803	0.10204781887084025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.812496346357668E-4	0.0	0.0	0.0	0.0
8	5.083328461810224E-4	0.0	0.0	0.0	0.0
9	7.624992692715336E-4	0.0	0.0	0.0	0.0
10	0.005083328461810224	0.0	0.0	0.0	0.0
11	0.010929156192891983	0.0	0.0	0.0	0.0
12	0.015122902173885417	0.0	0.0	0.0	0.0
13	0.019189564943333594	0.0	0.0	0.0	0.0
14	0.024399976616689075	0.0	0.0	0.0	0.0
15	0.029610388290044556	0.0	0.0	0.0	0.0
16	0.0374895474058504	0.0	0.0	0.0	0.0
17	0.042064543021479606	0.0	0.0	0.0	0.0
18	0.0463853722140183	0.0	0.0	0.0	0.0
19	0.05401036490673363	0.0	0.0	0.0	0.0
20	0.05871244373390809	0.0	0.0	0.0	0.0
21	0.06417702183035408	0.0	0.0	0.0	0.0
22	0.06887910065752853	0.0	0.0	0.0	0.0
23	0.0724374305807957	0.0	0.0	0.0	0.0
24	0.07802909188878694	0.0	0.0	0.0	0.0
25	0.08463741888914023	0.0	0.0	0.0	0.0
26	0.09010199698558623	0.0	0.0	0.0	0.0
27	0.09683740719748476	0.0	0.0	0.0	0.0
28	0.10369990062092857	0.0	0.0	0.0	0.0
29	0.10929156192891981	0.0	0.0	0.0	0.0
30	0.11539155608309208	0.0	0.0	0.0	0.0
31	0.12352488162198845	0.0	0.0	0.0	0.0
32	0.13331028891097313	0.0	0.0	0.0	0.0
33	0.13902903343050962	0.0	0.0	0.0	0.0
34	0.14601861006549868	0.0	0.0	0.0	0.0
35	0.1533894363351235	0.0	0.0	0.0	0.0
36	0.16177692829711038	0.0	0.0	0.0	0.0
37	0.16825817208591842	0.0	0.0	0.0	0.0
38	0.17588316477863375	0.0	0.0	0.0	0.0
39	0.18223732535589654	0.0	0.0	0.0	0.0
40	0.1885914859331593	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.01901605E-7	46.000004	27
AACGTAC	20	6.310234E-4	46.000004	13
GTCGAAA	20	6.310234E-4	46.000004	19
CCGATTT	35	1.01901605E-7	46.000004	37
TTAGCAC	20	6.310234E-4	46.000004	39
ACGCACA	80	0.0	46.000004	35
ATTACCC	35	1.01901605E-7	46.000004	45
CCGGATA	20	6.310234E-4	46.000004	17
GAATCGT	40	5.6079443E-9	46.000004	12
TGTGCTA	35	1.01901605E-7	46.000004	27
CATTAGG	35	1.01901605E-7	46.000004	20
TATTGCG	20	6.310234E-4	46.000004	31
AAACGAC	20	6.310234E-4	46.000004	22
AACGCAT	20	6.310234E-4	46.000004	22
CATACGT	20	6.310234E-4	46.000004	14
TCCGTAA	20	6.310234E-4	46.000004	42
CGATGCA	20	6.310234E-4	46.000004	34
ACGTAGG	35	1.01901605E-7	46.000004	28
TCATACG	20	6.310234E-4	46.000004	13
CGCACAT	20	6.310234E-4	46.000004	46
>>END_MODULE