SRR1527360_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406089
Sequences flagged as poor quality	0
Sequence length	50
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1900	0.46787773123625603	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1415	0.3484457840522644	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1234	0.3038742738660737	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1228	0.3023967652411171	No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC	701	0.17262225768242923	No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGA	682	0.16794348037006665	No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	647	0.15932468005781983	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	641	0.15784717143286325	No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACT	632	0.15563090849542835	No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCC	621	0.1529221426830079	No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCT	618	0.1521833883705296	No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTG	607	0.1494746225581092	No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGT	602	0.14824336537064536	No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT	595	0.146519605308196	No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATA	588	0.14479584524574662	No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGT	587	0.14454959380825386	No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACG	577	0.1420870794333262	No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAA	556	0.1369157992459781	No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCG	553	0.1361770449334998	No Hit
GAATTAGGGCCTTTCGCTGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAG	551	0.13568454205851427	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	537	0.13223702193361553	No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATA	535	0.13174451905863	No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGC	522	0.12854325037122405	No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC	518	0.12755824462125298	No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAA	514	0.1265732388712819	No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGAC	511	0.1258344845588036	No Hit
GAATTAGGGACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGC	498	0.12263321587139765	No Hit
GAATTAGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC	496	0.12214071299641212	No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCA	494	0.12164821012142657	No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGA	488	0.12017070149646998	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	478	0.11770818712154231	No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCA	475	0.11696943280906401	No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAA	469	0.11549192418410742	No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAG	468	0.11524567274661467	No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCC	467	0.11499942130912189	No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAA	467	0.11499942130912189	No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGA	462	0.11376816412165806	No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAA	456	0.11229065549670145	No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCAT	439	0.10810438105932443	No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCC	429	0.10564186668439678	No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTG	419	0.1031793523094691	No Hit
GAATTAGGGAGTGGGAGCAGGACTTCTGAAGAGCTGACTAGAACCGTCTA	419	0.1031793523094691	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.850459E-4	44.000004	29
AACGTCA	40	8.289135E-9	44.000004	36
GTACCGT	20	7.850459E-4	44.000004	26
TCGCACG	20	7.850459E-4	44.000004	38
ACACGAC	20	7.850459E-4	44.000004	40
CGTATTG	20	7.850459E-4	44.000004	13
GCGACAA	20	7.850459E-4	44.000004	18
CGAGTTT	20	7.850459E-4	44.000004	19
ATAGGCA	20	7.850459E-4	44.000004	18
CAACGGT	20	7.850459E-4	44.000004	17
ACCATTC	20	7.850459E-4	44.000004	37
ACGCATG	20	7.850459E-4	44.000004	40
CCGATGC	20	7.850459E-4	44.000004	34
CGTGCAA	20	7.850459E-4	44.000004	35
TAGACGA	20	7.850459E-4	44.000004	27
TTTCGGT	20	7.850459E-4	44.000004	42
CGGTTTA	80	0.0	44.000004	42
CGCATTT	20	7.850459E-4	44.000004	22
TTTCGAA	20	7.850459E-4	44.000004	13
CATTGCG	20	7.850459E-4	44.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content