##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406089
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.817074582172875	31.0	31.0	33.0	28.0	33.0
2	28.37974680427197	30.0	28.0	31.0	23.0	33.0
3	31.224564073392777	31.0	31.0	33.0	30.0	33.0
4	35.505416300367656	35.0	35.0	37.0	35.0	37.0
5	35.93429519144818	37.0	35.0	37.0	35.0	37.0
6	34.80760867691565	35.0	35.0	37.0	33.0	37.0
7	36.01392798130459	37.0	35.0	37.0	35.0	37.0
8	36.56592520358838	37.0	37.0	37.0	35.0	37.0
9	38.63162262459707	39.0	39.0	39.0	39.0	39.0
10	37.281497405741106	39.0	37.0	39.0	34.0	39.0
11	37.11235221835607	39.0	37.0	39.0	34.0	39.0
12	37.122497777580776	39.0	37.0	39.0	34.0	39.0
13	37.13954083956965	39.0	37.0	39.0	34.0	39.0
14	38.064453851249354	40.0	38.0	40.0	34.0	40.0
15	38.06046704047635	40.0	38.0	40.0	34.0	40.0
16	38.15972360738656	40.0	38.0	40.0	35.0	40.0
17	38.10230023467762	40.0	38.0	40.0	34.0	40.0
18	37.94417972414914	40.0	38.0	40.0	34.0	40.0
19	37.88678836412708	40.0	37.0	40.0	34.0	40.0
20	37.75560776086031	39.0	37.0	40.0	34.0	40.0
21	37.61515086594318	39.0	37.0	40.0	34.0	40.0
22	37.39442338994654	39.0	36.0	40.0	33.0	40.0
23	37.21728980592924	38.0	36.0	40.0	33.0	40.0
24	36.973067480281415	38.0	36.0	40.0	33.0	40.0
25	36.72865554102672	38.0	36.0	40.0	32.0	40.0
26	36.235775901341825	38.0	35.0	39.0	31.0	40.0
27	36.099475730689576	38.0	35.0	39.0	31.0	40.0
28	35.945814833694094	38.0	35.0	39.0	31.0	40.0
29	35.70548574327302	37.0	34.0	39.0	31.0	40.0
30	35.35728867317263	37.0	34.0	38.0	30.0	40.0
31	35.25816015700992	37.0	34.0	38.0	30.0	40.0
32	35.15858346323097	36.0	34.0	38.0	30.0	40.0
33	34.73866319944643	36.0	33.0	38.0	29.0	39.0
34	34.28899576201276	36.0	33.0	38.0	29.0	39.0
35	34.075495765706535	35.0	32.0	38.0	28.0	39.0
36	33.78282100721763	35.0	32.0	38.0	27.0	39.0
37	33.53704483499922	35.0	32.0	38.0	27.0	39.0
38	33.02603862700049	34.0	31.0	37.0	26.0	38.0
39	32.294186742315105	34.0	30.0	37.0	24.0	38.0
40	31.916680826124324	34.0	30.0	36.0	24.0	38.0
41	31.757316745836505	33.0	30.0	36.0	24.0	38.0
42	31.481145758688367	33.0	29.0	36.0	23.0	38.0
43	30.920985301251697	33.0	29.0	35.0	23.0	38.0
44	30.572349903592563	32.0	28.0	35.0	23.0	37.0
45	30.056652605709587	32.0	27.0	35.0	21.0	37.0
46	29.780949496292685	31.0	27.0	35.0	21.0	37.0
47	29.315049164099495	31.0	26.0	34.0	20.0	37.0
48	28.809334899492477	31.0	26.0	34.0	19.0	36.0
49	28.337497445141334	30.0	25.0	33.0	18.0	36.0
50	32.02327076084307	34.0	30.0	37.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	7.0
15	21.0
16	43.0
17	94.0
18	131.0
19	273.0
20	361.0
21	601.0
22	841.0
23	1163.0
24	1551.0
25	2112.0
26	2874.0
27	4253.0
28	5720.0
29	7543.0
30	9941.0
31	14629.0
32	22607.0
33	32818.0
34	52502.0
35	109145.0
36	118203.0
37	18241.0
38	403.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.37944637751824	0.4799440516734016	0.08249423156007674	0.05811533924829286
8	99.39840773820518	0.48560783473573527	0.08766551174742483	0.028318915311668132
9	98.75495273203657	0.8754238602867843	0.18764359536948796	0.18197981230715435
10	58.78268064389825	23.817931537175348	9.124600764856964	8.274787054069428
11	31.864689760126474	31.756831630504646	22.395090731342144	13.983387878026738
12	29.208375503892004	26.371312692537842	24.571460935903215	19.84885086766694
13	25.1302054475743	25.520760227437826	25.26515123532034	24.083883089667534
14	26.69464082996584	24.289256788536502	26.763837483901316	22.25226489759634
15	27.313470692385167	21.464260297619486	25.445899790440023	25.776369219555317
16	26.084183516421277	24.33235079009774	25.39467949144153	24.188786202039456
17	29.208621755329496	22.79771183164282	23.112421168758573	24.881245244269113
18	28.16229939742273	23.595073986244394	23.197870417568563	25.04475619876431
19	25.90466621848905	25.326467843256033	23.603200283681655	25.16566565457326
20	27.400151198382623	24.256505347349965	23.287998443690917	25.055345010576502
21	27.150944743639943	24.84258376858275	23.973316194233284	24.033155293544027
22	28.1667319232976	24.24714779272524	23.897963254360498	23.68815702961666
23	27.324305755634846	24.458677777531527	23.52883234955884	24.688184117274783
24	26.998515103831917	25.460428625252103	24.614554937464447	22.926501333451537
25	25.920426310488587	24.235820226600573	24.177458635914785	25.66629482699605
26	26.64366678240484	24.936405566267496	24.929510526017694	23.49041712530997
27	25.519282718812867	24.259460364599878	23.985382514670427	26.23587440191682
28	26.91700587802181	24.86425389508211	24.04842288266858	24.170317344227495
29	27.319380726884994	24.874842706894302	24.198390008101672	23.607386558119035
30	26.878836905210434	24.1599747838528	23.015151850948932	25.946036459987837
31	27.748596982434886	25.1607406258234	22.189963283910668	24.90069910783104
32	26.00686056504855	26.272812117540735	24.334320801597677	23.386006515813033
33	29.660246891691227	25.999473021923762	22.404694537404364	21.935585548980644
34	25.902942458426605	26.628645444717787	24.64041133840119	22.82800075845443
35	25.718746383182012	26.092802316733525	24.797766006959066	23.390685293125397
36	28.3201465688556	25.29593266500693	23.504945960122043	22.87897480601543
37	28.158113122985355	26.596632757843725	22.01019973454095	23.23505438462997
38	26.976598725895062	27.900780370805418	22.51846270152602	22.6041582017735
39	29.16897527389316	24.8135260989586	22.451482310527986	23.566016316620246
40	25.238309828633625	26.008584325111002	24.25601284447498	24.4970930017804
41	24.70369795783683	26.8561817729611	24.026999007606705	24.413121261595364
42	25.101640280825137	23.70859589892856	24.858343860582284	26.331419959664014
43	25.00831098601538	25.523468993250248	25.12207915013704	24.34614087059733
44	24.377661054596405	24.17056359566499	26.40849665959925	25.043278690139353
45	26.11028616879551	24.370519762909115	25.773414202305407	23.74577986598997
46	25.53578156512489	25.434818475752852	24.181891161789657	24.847508797332605
47	25.74706529849368	25.5830618411235	23.38822277875047	25.28165008163235
48	25.056083764888974	27.06155547183007	24.082651832480074	23.799708930800882
49	24.28211549684921	26.706953401840483	24.548313300778894	24.46261780053141
50	24.78028215489708	25.491702557813685	24.33161203578526	25.39640325150398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	16.0
6	31.0
7	129.5
8	228.0
9	312.0
10	396.0
11	494.5
12	593.0
13	577.5
14	562.0
15	573.0
16	584.0
17	654.5
18	725.0
19	912.5
20	1100.0
21	1531.5
22	1963.0
23	2161.0
24	2359.0
25	2803.0
26	3247.0
27	3756.0
28	4265.0
29	5003.5
30	5742.0
31	6496.0
32	7250.0
33	7296.0
34	7342.0
35	9121.0
36	10900.0
37	11761.5
38	12623.0
39	14042.0
40	15461.0
41	16520.0
42	17579.0
43	19304.0
44	21029.0
45	24474.5
46	27920.0
47	27747.5
48	27575.0
49	30415.0
50	33255.0
51	33566.0
52	33877.0
53	35736.0
54	37595.0
55	33885.5
56	30176.0
57	28923.0
58	27670.0
59	26440.5
60	25211.0
61	21780.5
62	18350.0
63	15370.5
64	12391.0
65	9424.5
66	6458.0
67	5588.5
68	4719.0
69	3835.5
70	2952.0
71	2517.5
72	2083.0
73	1499.0
74	915.0
75	870.5
76	826.0
77	469.5
78	113.0
79	67.5
80	22.0
81	11.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	406089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.998628379493166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6502102830296	9.573517135406277
2	8.922820444814914	2.319688541181859
3	3.076573333838518	1.1997370034647576
4	1.462508998598113	0.7604244389776625
5	0.7464100329632857	0.48511533186074973
6	0.5626491872845072	0.4388200616121097
7	0.3826772250217861	0.34819953261477166
8	0.2690107225400674	0.27974163299178256
9	0.303110673284583	0.35460206998958355
>10	6.7669457810783165	24.020350218794402
>50	2.415413177736521	21.700169174737557
>100	1.3924146554010535	33.92162801750355
>500	0.04167771757663016	3.1754122864692222
>1k	0.007577766832114576	1.4225945543957113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1900	0.46787773123625603	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1415	0.3484457840522644	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1234	0.3038742738660737	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1228	0.3023967652411171	No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC	701	0.17262225768242923	No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGA	682	0.16794348037006665	No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	647	0.15932468005781983	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	641	0.15784717143286325	No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACT	632	0.15563090849542835	No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCC	621	0.1529221426830079	No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCT	618	0.1521833883705296	No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTG	607	0.1494746225581092	No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGT	602	0.14824336537064536	No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT	595	0.146519605308196	No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATA	588	0.14479584524574662	No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGT	587	0.14454959380825386	No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACG	577	0.1420870794333262	No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAA	556	0.1369157992459781	No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCG	553	0.1361770449334998	No Hit
GAATTAGGGCCTTTCGCTGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAG	551	0.13568454205851427	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	537	0.13223702193361553	No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATA	535	0.13174451905863	No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGC	522	0.12854325037122405	No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC	518	0.12755824462125298	No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAA	514	0.1265732388712819	No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGAC	511	0.1258344845588036	No Hit
GAATTAGGGACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGC	498	0.12263321587139765	No Hit
GAATTAGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC	496	0.12214071299641212	No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCA	494	0.12164821012142657	No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGA	488	0.12017070149646998	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	478	0.11770818712154231	No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCA	475	0.11696943280906401	No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAA	469	0.11549192418410742	No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAG	468	0.11524567274661467	No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCC	467	0.11499942130912189	No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAA	467	0.11499942130912189	No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGA	462	0.11376816412165806	No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAA	456	0.11229065549670145	No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCAT	439	0.10810438105932443	No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCC	429	0.10564186668439678	No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTG	419	0.1031793523094691	No Hit
GAATTAGGGAGTGGGAGCAGGACTTCTGAAGAGCTGACTAGAACCGTCTA	419	0.1031793523094691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.026102652374233235	0.0	0.0	0.0	0.0
7	0.026595155249218766	0.0	0.0	0.0	0.0
8	0.028072663874175365	0.0	0.0	0.0	0.0
9	0.030288926811610262	0.0	0.0	0.0	0.0
10	0.04752652743610391	0.0	0.0	0.0	0.0
11	0.086434254559961	0.0	0.0	0.0	0.0
12	0.1039181066219474	0.0	0.0	0.0	0.0
13	0.11598442705909295	0.0	0.0	0.0	0.0
14	0.12829699893373128	0.0	0.0	0.0	0.0
15	0.1450420966832394	0.0	0.0	0.0	0.0
16	0.15932468005781983	0.0	0.0	0.0	0.0
17	0.17188350336995092	0.0	0.0	0.0	0.0
18	0.18419607524458925	0.0	0.0	0.0	0.0
19	0.19626239568173479	0.0	0.0	0.0	0.0
20	0.21276124199375013	0.0	0.0	0.0	0.0
21	0.22458131099340292	0.0	0.0	0.0	0.0
22	0.23369261418063528	0.0	0.0	0.0	0.0
23	0.25068396336763615	0.0	0.0	0.0	0.0
24	0.26595155249218766	0.0	0.0	0.0	0.0
25	0.28072663874175363	0.0	0.0	0.0	0.0
26	0.29771798792875453	0.0	0.0	0.0	0.0
27	0.3198806173031035	0.0	0.0	0.0	0.0
28	0.3459832696773367	0.0	0.0	0.0	0.0
29	0.36198961311436656	0.0	0.0	0.0	0.0
30	0.3821822309887734	0.0	0.0	0.0	0.0
31	0.4011435916757164	0.0	0.0	0.0	0.0
32	0.41690368367525343	0.0	0.0	0.0	0.0
33	0.42872375267490626	0.0	0.0	0.0	0.0
34	0.44694635904937097	0.0	0.0	0.0	0.0
35	0.46467646254885014	0.0	0.0	0.0	0.0
36	0.481667811735851	0.0	0.0	0.0	0.0
37	0.4927491264230255	0.0	0.0	0.0	0.0
38	0.5050616982976638	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.850459E-4	44.000004	29
AACGTCA	40	8.289135E-9	44.000004	36
GTACCGT	20	7.850459E-4	44.000004	26
TCGCACG	20	7.850459E-4	44.000004	38
ACACGAC	20	7.850459E-4	44.000004	40
CGTATTG	20	7.850459E-4	44.000004	13
GCGACAA	20	7.850459E-4	44.000004	18
CGAGTTT	20	7.850459E-4	44.000004	19
ATAGGCA	20	7.850459E-4	44.000004	18
CAACGGT	20	7.850459E-4	44.000004	17
ACCATTC	20	7.850459E-4	44.000004	37
ACGCATG	20	7.850459E-4	44.000004	40
CCGATGC	20	7.850459E-4	44.000004	34
CGTGCAA	20	7.850459E-4	44.000004	35
TAGACGA	20	7.850459E-4	44.000004	27
TTTCGGT	20	7.850459E-4	44.000004	42
CGGTTTA	80	0.0	44.000004	42
CGCATTT	20	7.850459E-4	44.000004	22
TTTCGAA	20	7.850459E-4	44.000004	13
CATTGCG	20	7.850459E-4	44.000004	15
>>END_MODULE