FastQCFastQC Report
Fri 17 Jun 2016
SRR1527359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349665
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16520.47245220425264184No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12090.34575951267641886No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10980.3140148427780876No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10370.2965695737348605No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT6470.18503424706504798No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC5910.1690189181073313No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT5910.1690189181073313No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC5690.16272718173108547No Hit
GAATTAGGGCCTTTCGCTGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTA5580.15958131354296257No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT5510.15757939742324797No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG5510.15757939742324797No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCCCC5500.1572934094061459No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACTGA5410.15471951725222713No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC5310.1518596370812063No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCT5230.14957173294438963No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGTCT5030.14385197260234797No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACGAA4950.1415640684655313No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGCAG4880.1395621523458167No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGTCT4820.13784622424320422No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATATT4790.13698826019189794No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGC4740.13555832010638755No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAAAA4660.13327041596957087No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATAAA4570.1306965238156521No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC4540.12983855976434588No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA4530.12955257174724377No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGACAG4500.12869460769593755No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCAGG4370.12497676347361045No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAG4330.12383281140520212No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTGAG4250.12154490726838546No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAAGA4210.12040095519997712No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGAGG4160.1189710151144667No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAGAA4160.1189710151144667No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3980.11382323080662919No Hit
GAATTAGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACC3920.1121073027040167No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCCAA3900.11153532666981253No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAAGA3860.11039137460140419No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCATCC3810.1089614345158938No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAAGA3770.10781748244748546No Hit
GAATTAGGGGAGAAATGACCGCCTTTCTTACAACCAAAACAGTCCCTCTGCC3750.10724550641328129No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAACA3650.10438562624226044No Hit
GAATTAGGGAGTGGGAGCAGGACTTCTGAAGAGCTGACTAGAACCGTCTAGA3640.10409963822515837No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCCCT3630.10381365020805627No Hit
GAATTAGGGGTACATGAATGGCCATTCTACCCAGATAGGTTTTAGCTTATAT3510.10038179400283127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC253.412246E-546.018
TCGCCCG405.5915734E-946.024
TCGCCCC301.8576629E-646.040
ATTTACG301.8576629E-646.043
AGACTTA206.305402E-446.033
CGGGTAC301.8576629E-646.011
CCTTATC206.305402E-446.038
GATACGT301.8576629E-646.038
GGTACGA301.8576629E-646.031
GTACCGA301.8576629E-646.020
ACAGTCG253.412246E-546.036
GTCGCAG253.412246E-546.035
TCGCAGG301.8576629E-646.036
ACCGGCA301.8576629E-646.020
ACACGAC301.8576629E-646.040
ACGCCGC206.305402E-446.027
CATATGG301.8576629E-646.036
ATTAGCA405.5915734E-946.034
ATAGGTT206.305402E-446.035
CTACTAC301.8576629E-646.022
Produced by FastQC (version 0.11.5)