##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349665
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574770137131253	33.0	31.0	33.0	30.0	33.0
2	29.739633649350093	31.0	30.0	31.0	25.0	33.0
3	31.67508901377032	33.0	31.0	33.0	30.0	34.0
4	35.98606666380679	37.0	35.0	37.0	35.0	37.0
5	36.32477657186164	37.0	35.0	37.0	35.0	37.0
6	35.302543863412126	35.0	35.0	37.0	33.0	37.0
7	36.24324138818584	37.0	35.0	37.0	35.0	37.0
8	36.65642257589407	37.0	37.0	37.0	35.0	37.0
9	38.74656599888465	39.0	39.0	39.0	39.0	39.0
10	37.506450459725734	39.0	37.0	39.0	34.0	39.0
11	37.518347561237185	39.0	37.0	39.0	35.0	39.0
12	37.608019103999546	39.0	37.0	39.0	35.0	39.0
13	37.60384653883002	39.0	37.0	39.0	35.0	39.0
14	38.51582228704617	40.0	38.0	40.0	35.0	40.0
15	38.55260606580584	40.0	38.0	40.0	35.0	40.0
16	38.63268843035477	40.0	38.0	40.0	35.0	40.0
17	38.644339582171504	40.0	38.0	40.0	35.0	40.0
18	38.51312256016473	40.0	38.0	40.0	35.0	40.0
19	38.44417656900176	40.0	38.0	40.0	35.0	40.0
20	38.472149056954514	40.0	38.0	40.0	35.0	40.0
21	38.371020834227046	40.0	38.0	40.0	35.0	40.0
22	38.36206654941158	40.0	38.0	40.0	35.0	40.0
23	38.23874851643716	40.0	38.0	40.0	35.0	40.0
24	38.15222284186292	40.0	38.0	40.0	35.0	40.0
25	38.14932578324968	40.0	38.0	40.0	35.0	40.0
26	38.071233895299784	40.0	38.0	40.0	35.0	40.0
27	38.03838531165544	40.0	38.0	40.0	35.0	40.0
28	37.98451946863426	40.0	38.0	40.0	34.0	40.0
29	37.986458467390214	40.0	38.0	40.0	34.0	40.0
30	37.83567986501365	40.0	38.0	40.0	34.0	40.0
31	37.82484091916549	40.0	38.0	40.0	34.0	40.0
32	37.66674102355111	40.0	37.0	40.0	34.0	40.0
33	37.485044256645644	40.0	37.0	40.0	33.0	40.0
34	37.26716428581643	39.0	36.0	40.0	33.0	40.0
35	37.18305235010653	39.0	36.0	40.0	33.0	40.0
36	37.09716156893026	39.0	36.0	40.0	32.0	40.0
37	37.062268170963634	39.0	36.0	40.0	33.0	40.0
38	37.16080820213633	39.0	36.0	40.0	33.0	40.0
39	37.146431584516606	40.0	36.0	40.0	33.0	40.0
40	37.0151487852659	39.0	36.0	40.0	33.0	40.0
41	36.87567814908555	39.0	36.0	40.0	32.0	40.0
42	36.795484249209956	39.0	35.0	40.0	32.0	40.0
43	36.78457666623768	39.0	35.0	40.0	32.0	40.0
44	36.71342570746286	39.0	35.0	40.0	32.0	40.0
45	36.60684655312942	39.0	35.0	40.0	32.0	40.0
46	36.433938197989505	39.0	35.0	40.0	31.0	40.0
47	36.39413724564941	39.0	35.0	40.0	31.0	40.0
48	36.18641556918765	39.0	35.0	40.0	31.0	40.0
49	36.088587648177544	39.0	35.0	40.0	31.0	40.0
50	36.43168461241474	39.0	35.0	40.0	32.0	40.0
51	36.46656657086068	39.0	35.0	40.0	32.0	40.0
52	36.174752977850225	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	9.0
16	23.0
17	30.0
18	46.0
19	90.0
20	158.0
21	238.0
22	439.0
23	695.0
24	901.0
25	1092.0
26	1635.0
27	2251.0
28	2807.0
29	3119.0
30	3471.0
31	4447.0
32	6191.0
33	9421.0
34	19198.0
35	18986.0
36	30110.0
37	60294.0
38	159127.0
39	24882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.37340025452933	0.48360573691962305	0.08837029728454378	0.05462371126649793
8	99.43259977406946	0.4615846596027626	0.08179257289119586	0.024022993436575005
9	98.75709607767435	0.8854189009480503	0.19189795947549798	0.1655870619021063
10	58.93297870819213	23.78705332246579	9.082407447127967	8.197560522214118
11	32.02837001129652	31.561351579368825	22.39686557133256	14.013412838002088
12	29.39384839775213	26.16246979251569	24.579526117855664	19.864155691876512
13	25.29564011267928	25.371426937211332	25.440636037350036	23.892296912759356
14	26.93721133084524	24.139676547552657	26.712424749403002	22.210687372199107
15	27.246936353366795	21.564354453548397	25.5178528019676	25.670856391117212
16	26.13186907468577	24.387628158380164	25.18725065419759	24.29325211273648
17	29.367823488195842	22.457495030958203	23.377804469992707	24.796877010853247
18	28.341412494816463	23.33976806371813	23.071511303676377	25.247308137789027
19	25.87791171549912	25.34625999170635	23.524230334748975	25.251597958045558
20	27.34102641099338	24.079619063961218	23.437575965567042	25.141778559478357
21	27.05418042983999	24.85807844651309	23.913174038007806	24.17456708563911
22	28.462099438033544	24.094490440850528	23.856548410621595	23.58686171049433
23	27.315573477471293	24.45597929446756	23.378090458009808	24.850356770051334
24	27.192026654083197	25.17666909756481	24.681909827978206	22.949394420373785
25	25.9022206969528	24.18257475011797	24.097922297055753	25.81728225587348
26	26.482204395635822	24.661032702729756	25.204695923240816	23.652066978393606
27	25.713468605665422	24.080191039995423	23.924041582657686	26.282298771681468
28	27.160281984184863	24.67819198375588	24.14797020004862	24.01355583201064
29	27.33845251883946	24.632147913002445	24.38991606251698	23.639483505641113
30	27.08420917163571	23.878855475955557	23.007163999828407	26.029771352580326
31	27.959618491985182	24.782577609998143	22.404587247794318	24.853216650222357
32	26.175053265268183	26.13415697882259	24.269801095334106	23.42098866057512
33	29.62750060772454	25.861896386541407	22.588191554773854	21.922411450960205
34	26.01204009552	26.581442237570247	24.67504611556776	22.731471551342
35	25.927387642457784	25.94626285158652	24.718516294167276	23.407833211788425
36	28.476970814922854	25.28820442423462	23.40011153532667	22.834713225515852
37	28.248752377275395	26.41471122359973	22.083136716571573	23.2533996825533
38	27.308423777043743	27.80318304663035	22.435473953641342	22.45291922268457
39	29.273161454535057	24.701928989175354	22.50925886205368	23.515650694235912
40	25.74349734746114	25.73920752720461	24.077045171807303	24.440249953526948
41	24.764274376903607	26.725580198189697	23.967797749274304	24.542347675632392
42	25.4086053794346	23.461312970986516	24.866658087026153	26.263423562552727
43	25.2844865800123	25.201836043069797	25.263323466746744	24.250353910171164
44	24.6670384510889	23.889723020605437	26.448171821600674	24.99506670670499
45	26.429296612471937	24.059313914746973	25.68000800766448	23.83138146511661
46	25.617090643902017	25.158937840504482	24.193156306750748	25.03081520884275
47	25.799265010796045	25.392018074442678	23.605164943588864	25.203551971172406
48	25.219281312113022	26.909470493186337	24.084480860251954	23.786767334448687
49	24.537771867358757	26.17390931319978	24.83033760885419	24.457981210587278
50	24.821471980324024	25.30393376517524	24.365607081063303	25.50898717343743
51	24.67819198375588	25.843307165429767	25.470950767162854	24.007550083651495
52	25.473810647333877	24.888679164343014	25.593353638482547	24.04415654984056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.5
4	1.0
5	9.0
6	17.0
7	112.5
8	208.0
9	267.5
10	327.0
11	449.5
12	572.0
13	490.0
14	401.0
15	394.0
16	469.5
17	545.0
18	744.5
19	944.0
20	1002.0
21	1060.0
22	1280.0
23	1500.0
24	1701.0
25	1902.0
26	2247.0
27	2592.0
28	3824.0
29	5056.0
30	4981.0
31	4906.0
32	5799.0
33	6692.0
34	6468.0
35	6244.0
36	8118.0
37	9992.0
38	10665.0
39	11470.5
40	11603.0
41	13952.5
42	16302.0
43	17520.0
44	18738.0
45	19318.0
46	19898.0
47	22478.5
48	25059.0
49	25818.5
50	26578.0
51	28036.5
52	29495.0
53	28745.5
54	27996.0
55	27575.0
56	27154.0
57	25766.5
58	24379.0
59	21342.0
60	18305.0
61	18694.5
62	19084.0
63	15358.0
64	8980.5
65	6329.0
66	5060.5
67	3792.0
68	3739.5
69	3687.0
70	3157.5
71	2628.0
72	1899.5
73	1171.0
74	818.5
75	466.0
76	492.0
77	518.0
78	329.0
79	140.0
80	74.5
81	9.0
82	5.0
83	1.0
84	0.5
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	349665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.390902721175983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00251067034898	7.973917892840291
2	9.048455937735374	2.061401627271817
3	3.0379111222696458	1.0381365020805629
4	1.4310820989204118	0.6520526789927502
5	0.8988199849359779	0.5119185506127293
6	0.6603063017825759	0.4512890909870876
7	0.5423047953803666	0.4324138818583501
8	0.41928194828019083	0.38207999084838346
9	0.3615365302535777	0.37064047016430013
>10	9.216670851117248	28.0299715441923
>50	2.726587998995732	21.268642843864843
>100	1.6143610343961838	33.48090315015801
>500	0.030128044187798145	1.9178356426865713
>1k	0.010042681395932713	1.4287961334420087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1652	0.47245220425264184	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1209	0.34575951267641886	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1098	0.3140148427780876	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1037	0.2965695737348605	No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	647	0.18503424706504798	No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC	591	0.1690189181073313	No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT	591	0.1690189181073313	No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC	569	0.16272718173108547	No Hit
GAATTAGGGCCTTTCGCTGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTA	558	0.15958131354296257	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	551	0.15757939742324797	No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG	551	0.15757939742324797	No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCCCC	550	0.1572934094061459	No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACTGA	541	0.15471951725222713	No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	531	0.1518596370812063	No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCT	523	0.14957173294438963	No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGTCT	503	0.14385197260234797	No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACGAA	495	0.1415640684655313	No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGCAG	488	0.1395621523458167	No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGTCT	482	0.13784622424320422	No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATATT	479	0.13698826019189794	No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGC	474	0.13555832010638755	No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAAAA	466	0.13327041596957087	No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATAAA	457	0.1306965238156521	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	454	0.12983855976434588	No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA	453	0.12955257174724377	No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGACAG	450	0.12869460769593755	No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCAGG	437	0.12497676347361045	No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAG	433	0.12383281140520212	No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTGAG	425	0.12154490726838546	No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAAGA	421	0.12040095519997712	No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGAGG	416	0.1189710151144667	No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAGAA	416	0.1189710151144667	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	398	0.11382323080662919	No Hit
GAATTAGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACC	392	0.1121073027040167	No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCCAA	390	0.11153532666981253	No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAAGA	386	0.11039137460140419	No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCATCC	381	0.1089614345158938	No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAAGA	377	0.10781748244748546	No Hit
GAATTAGGGGAGAAATGACCGCCTTTCTTACAACCAAAACAGTCCCTCTGCC	375	0.10724550641328129	No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAACA	365	0.10438562624226044	No Hit
GAATTAGGGAGTGGGAGCAGGACTTCTGAAGAGCTGACTAGAACCGTCTAGA	364	0.10409963822515837	No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCCCT	363	0.10381365020805627	No Hit
GAATTAGGGGTACATGAATGGCCATTCTACCCAGATAGGTTTTAGCTTATAT	351	0.10038179400283127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03002874179571876	0.0	0.0	0.0	0.0
7	0.03088670584702501	0.0	0.0	0.0	0.0
8	0.031172693864127094	0.0	0.0	0.0	0.0
9	0.03260263394963751	0.0	0.0	0.0	0.0
10	0.05433772324939585	0.0	0.0	0.0	0.0
11	0.09923784193442295	0.0	0.0	0.0	0.0
12	0.12383281140520212	0.0	0.0	0.0	0.0
13	0.13841820027740837	0.0	0.0	0.0	0.0
14	0.15586346932063547	0.0	0.0	0.0	0.0
15	0.17445269043227088	0.0	0.0	0.0	0.0
16	0.19389987559521257	0.0	0.0	0.0	0.0
17	0.20934322851872506	0.0	0.0	0.0	0.0
18	0.22450059342513548	0.0	0.0	0.0	0.0
19	0.23822801824603548	0.0	0.0	0.0	0.0
20	0.25109747901562923	0.0	0.0	0.0	0.0
21	0.26253699969971256	0.0	0.0	0.0	0.0
22	0.28198418486265425	0.0	0.0	0.0	0.0
23	0.2968555617519626	0.0	0.0	0.0	0.0
24	0.31029699855576054	0.0	0.0	0.0	0.0
25	0.3274562795818855	0.0	0.0	0.0	0.0
26	0.3420416684540918	0.0	0.0	0.0	0.0
27	0.35462514120658345	0.0	0.0	0.0	0.0
28	0.3743583143866272	0.0	0.0	0.0	0.0
29	0.3849398710194043	0.0	0.0	0.0	0.0
30	0.3969513677376918	0.0	0.0	0.0	0.0
31	0.41010681652438763	0.0	0.0	0.0	0.0
32	0.4226902892768793	0.0	0.0	0.0	0.0
33	0.43384382194386056	0.0	0.0	0.0	0.0
34	0.4478572347818626	0.0	0.0	0.0	0.0
35	0.4655884918421918	0.0	0.0	0.0	0.0
36	0.4827477728683168	0.0	0.0	0.0	0.0
37	0.49104142536427725	0.0	0.0	0.0	0.0
38	0.5007650179457481	0.0	0.0	0.0	0.0
39	0.5110605865614231	0.0	0.0	0.0	0.0
40	0.5302217837072627	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	25	3.412246E-5	46.0	18
TCGCCCG	40	5.5915734E-9	46.0	24
TCGCCCC	30	1.8576629E-6	46.0	40
ATTTACG	30	1.8576629E-6	46.0	43
AGACTTA	20	6.305402E-4	46.0	33
CGGGTAC	30	1.8576629E-6	46.0	11
CCTTATC	20	6.305402E-4	46.0	38
GATACGT	30	1.8576629E-6	46.0	38
GGTACGA	30	1.8576629E-6	46.0	31
GTACCGA	30	1.8576629E-6	46.0	20
ACAGTCG	25	3.412246E-5	46.0	36
GTCGCAG	25	3.412246E-5	46.0	35
TCGCAGG	30	1.8576629E-6	46.0	36
ACCGGCA	30	1.8576629E-6	46.0	20
ACACGAC	30	1.8576629E-6	46.0	40
ACGCCGC	20	6.305402E-4	46.0	27
CATATGG	30	1.8576629E-6	46.0	36
ATTAGCA	40	5.5915734E-9	46.0	34
ATAGGTT	20	6.305402E-4	46.0	35
CTACTAC	30	1.8576629E-6	46.0	22
>>END_MODULE