FastQCFastQC Report
Fri 17 Jun 2016
SRR1527358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402284
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19430.48299211502321737No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14740.3664078113969236No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12210.30351691839595907No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11450.28462479243519506No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT7210.17922661602251147No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT7000.17400642332282665No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC6580.16356603792345706No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC6450.16033449006174744No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATATT6270.1558600391763033No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACTGA6060.15063984647661852No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6040.1501426852671247No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG6020.14964552405763093No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGTCT5910.1469111374054151No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGTCT5890.14641397619592128No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACGAA5850.14541965377693372No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGC5780.14367958954370544No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATAAA5690.1414423641009834No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCT5620.13970229986775512No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAAAA5610.13945371926300823No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGCAG5530.13746507442503306No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAAGA5420.13473068777281721No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC5410.13448210716807033No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCCCC5400.13423352656332344No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGAGG5390.13398494595857652No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA5210.12951049507313242No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCCCT5190.1290133338636386No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAAGA5140.12777043083990414No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC5130.12752185023515725No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCCAA5130.12752185023515725No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCAGG5050.1255332053971821No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG4910.12205307693072555No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGACAG4720.11733004544053456No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAG4680.11633572302154696No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTGAG4660.11583856181205318No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAAGA4620.11484423939306561No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAACA4610.1145956587883187No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAGAA4530.11260701395034353No Hit
GAATTAGGGACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCAC4240.10539817641268359No Hit
GAATTAGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGA4110.10216662855097394No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCATCC4100.10191804794622704No Hit
GAATTAGGGGGACTGATCAGTAAGATGGCCCATTATTGAATCGAGTCGAAAA4040.10042656431774567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG351.0173608E-746.00000424
TCGCAAA351.0173608E-746.00000432
CAAACGA351.0173608E-746.00000445
AGATACG351.0173608E-746.00000437
TTTCGAC351.0173608E-746.00000444
ATTACTC351.0173608E-746.00000416
AACCACG351.0173608E-746.00000421
TGTATCG351.0173608E-746.00000423
CCGTCTA351.0173608E-746.00000444
AATGCCG351.0173608E-746.00000416
CTCGCAA351.0173608E-746.00000431
CATAATG351.0173608E-746.00000444
CGATGCA351.0173608E-746.00000429
CCGAAGG351.0173608E-746.00000432
GCGTGCT351.0173608E-746.00000412
CGGTAGT700.046.00000412
CCTATAT351.0173608E-746.00000437
CTTAGTA351.0173608E-746.00000429
TATCGCG351.0173608E-746.00000425
CCCGCTA351.0173608E-746.00000445
Produced by FastQC (version 0.11.5)