##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	402284
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.220366706108123	31.0	30.0	33.0	27.0	33.0
2	28.516662357936184	30.0	28.0	31.0	25.0	33.0
3	30.928388899384515	31.0	31.0	33.0	28.0	33.0
4	35.376922770977714	35.0	35.0	37.0	33.0	37.0
5	35.833525569001004	37.0	35.0	37.0	35.0	37.0
6	34.61094649551063	35.0	35.0	37.0	32.0	37.0
7	35.910453808752024	37.0	35.0	37.0	35.0	37.0
8	36.45765180817532	37.0	37.0	37.0	35.0	37.0
9	38.61755625379085	39.0	39.0	39.0	38.0	39.0
10	37.02773662387766	39.0	37.0	39.0	32.0	39.0
11	36.88229708365235	39.0	37.0	39.0	34.0	39.0
12	36.9662651261298	39.0	37.0	39.0	34.0	39.0
13	36.974624891867435	39.0	37.0	39.0	33.0	39.0
14	37.88906841932565	40.0	38.0	40.0	33.0	40.0
15	37.95216066261646	40.0	38.0	40.0	34.0	40.0
16	37.99674359407781	40.0	38.0	40.0	34.0	40.0
17	38.06076304302433	40.0	38.0	40.0	34.0	40.0
18	37.95878284992692	40.0	38.0	40.0	34.0	40.0
19	37.852899941334975	40.0	37.0	40.0	34.0	40.0
20	37.84458740591224	40.0	37.0	40.0	34.0	40.0
21	37.82678654880632	40.0	37.0	40.0	34.0	40.0
22	37.80818277634706	40.0	37.0	40.0	34.0	40.0
23	37.77213610285271	40.0	37.0	40.0	34.0	40.0
24	37.67321593699973	39.0	37.0	40.0	34.0	40.0
25	37.633967545316246	39.0	37.0	40.0	34.0	40.0
26	37.53279026757216	39.0	37.0	40.0	33.0	40.0
27	37.48668602280976	39.0	37.0	40.0	33.0	40.0
28	37.35959421702081	39.0	36.0	40.0	33.0	40.0
29	37.39084577064959	39.0	36.0	40.0	33.0	40.0
30	37.29530878682721	39.0	36.0	40.0	33.0	40.0
31	37.21870121605632	39.0	36.0	40.0	33.0	40.0
32	37.11627109206431	39.0	36.0	40.0	33.0	40.0
33	37.01398763062911	39.0	36.0	40.0	32.0	40.0
34	36.84017261437194	39.0	35.0	40.0	32.0	40.0
35	36.73678545505165	39.0	35.0	40.0	31.0	40.0
36	36.662415109723476	39.0	35.0	40.0	31.0	40.0
37	36.60943263962773	38.0	35.0	40.0	31.0	40.0
38	36.49550069105408	38.0	35.0	40.0	31.0	40.0
39	36.41549750922234	38.0	35.0	40.0	31.0	40.0
40	36.65578546499488	39.0	35.0	40.0	31.0	40.0
41	36.747715544242375	39.0	35.0	40.0	32.0	40.0
42	36.815938491215164	39.0	35.0	40.0	32.0	40.0
43	36.8164381382307	39.0	35.0	40.0	32.0	40.0
44	36.72339441787394	39.0	35.0	40.0	32.0	40.0
45	36.64648357876525	39.0	35.0	40.0	32.0	40.0
46	36.3371647890545	39.0	35.0	40.0	31.0	40.0
47	36.36894333356534	39.0	35.0	40.0	31.0	40.0
48	36.25690059758777	38.0	35.0	40.0	31.0	40.0
49	36.155835181115826	38.0	35.0	40.0	31.0	40.0
50	36.04865468176711	38.0	35.0	40.0	31.0	40.0
51	35.909432142466514	38.0	35.0	40.0	31.0	40.0
52	35.495359000109374	38.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	4.0
14	8.0
15	9.0
16	20.0
17	56.0
18	62.0
19	139.0
20	256.0
21	385.0
22	673.0
23	858.0
24	1153.0
25	1606.0
26	2143.0
27	2994.0
28	3870.0
29	4755.0
30	5717.0
31	7342.0
32	10198.0
33	15440.0
34	26143.0
35	27189.0
36	43622.0
37	80823.0
38	154456.0
39	12358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.39097751837011	0.448439410963399	0.12205307693072555	0.03852999373576876
8	99.44317944536695	0.4362589613308011	0.08700321166141332	0.03355838164083086
9	98.7829493591592	0.8598403118195106	0.17574648755605493	0.1814638414652335
10	58.96530809080153	23.846088832764913	9.102276004017062	8.086327072416502
11	31.80812560280797	31.674637818058883	22.510465243459844	14.006771335673305
12	29.05956985612155	26.238925734058526	24.700211790675244	20.001292619144685
13	24.942080719093973	25.36292768293047	25.444213540682703	24.25077805729286
14	26.513110141094355	23.99846874347476	26.96701832536218	22.521402790068706
15	27.43807857135755	21.244692804088654	25.373865229539334	25.94336339501447
16	26.070139503435385	24.238349027055513	25.31395730379533	24.377554165713775
17	29.13911564964055	22.566644460132643	23.38497181095942	24.90926807926738
18	28.226079088405204	23.307663242883137	23.24899822016287	25.217259448548784
19	26.00575712680594	25.186186872955425	23.614411709140807	25.193644291097833
20	27.333674717363856	24.054150798938064	23.155034751568543	25.45713973212954
21	26.878026468862792	24.956498394169294	23.89431347008581	24.271161666882104
22	28.061767308667505	24.18415845522069	24.03277286692983	23.721301369181973
23	27.323731493173973	24.354933330681806	23.327052530053393	24.99428264609082
24	27.1696115182309	25.407672191784908	24.54112020363723	22.88159608634696
25	25.997305386244545	24.09292937327858	24.03376718934882	25.875998051128057
26	26.46264827833073	24.836185381471797	25.18991558202663	23.511250758170846
27	25.734307106422328	24.076523053365285	23.85106044485985	26.33810939535254
28	26.905121754780204	24.83444531723857	24.000954549522227	24.259478378458997
29	27.24741724751668	24.631603543765102	24.36139642640523	23.759582782312993
30	26.89443278877609	23.87194121565859	23.0344731582663	26.19915283729902
31	27.794543158564593	25.10092372552724	22.2377723200525	24.866760795855665
32	26.004514223782206	26.16559445565819	24.30049417824224	23.529397142317368
33	29.693201817621386	25.870032116614134	22.324029789899676	22.112736275864812
34	25.803909675751456	26.635660379234572	24.760616877628742	22.79981306738523
35	25.995565322011316	25.951815135575863	24.71413230454107	23.338487237871753
36	28.282755466287497	25.27169860098836	23.397400841196767	23.048145091527378
37	28.155730777261834	26.517584591979798	22.053076930725556	23.273607700032812
38	27.05650734307106	28.009316801065914	22.393632359228803	22.540543496634218
39	29.026757216294957	24.812570224020842	22.34192759344145	23.818744966242754
40	25.351741555716856	25.883206888665715	24.38998319595112	24.375068359666304
41	24.77354306907558	26.80742957711468	23.926877529307653	24.492149824502093
42	24.97290471408259	23.66462499129968	24.852840281989838	26.509630012627895
43	25.16878623062314	25.276421632478545	25.215022223106065	24.339769913792246
44	24.628869157112884	24.076771633970033	26.21879070507403	25.075568503843055
45	26.23171689652087	24.014626482783306	25.932177267800853	23.82147935289497
46	25.651281184436865	25.19762158077378	24.242574897336212	24.908522337453142
47	25.855614441538815	25.20657048254467	23.38497181095942	25.552843264957094
48	25.067613924491155	27.048304183114418	24.26668721599666	23.61739467639777
49	24.549820524803373	26.395780095653816	24.719352497240756	24.335046882302056
50	24.75266229827684	25.387288582195662	24.2356146404033	25.624434479124204
51	24.772797327261337	25.635372025733066	25.22571118911018	24.366119457895415
52	25.447445088544413	24.945809428165177	25.557317715842537	24.049427767447874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.0
4	3.0
5	17.5
6	32.0
7	136.5
8	241.0
9	323.0
10	405.0
11	489.5
12	574.0
13	529.5
14	476.0
15	467.0
16	528.5
17	590.0
18	787.5
19	985.0
20	1120.5
21	1256.0
22	1525.5
23	1795.0
24	2012.5
25	2230.0
26	2647.5
27	3065.0
28	4359.5
29	5654.0
30	5690.0
31	5726.0
32	6545.5
33	7365.0
34	7149.0
35	6933.0
36	9012.0
37	11091.0
38	12218.0
39	13411.0
40	13477.0
41	16222.0
42	18967.0
43	20283.5
44	21600.0
45	22521.0
46	23442.0
47	26239.5
48	29037.0
49	30060.5
50	31084.0
51	32413.0
52	33742.0
53	32901.0
54	32060.0
55	31713.5
56	31367.0
57	29654.0
58	27941.0
59	24621.0
60	21301.0
61	21385.0
62	21469.0
63	17417.5
64	10271.0
65	7176.0
66	5837.5
67	4499.0
68	4364.5
69	4230.0
70	3540.5
71	2851.0
72	2057.0
73	1263.0
74	1013.0
75	763.0
76	548.5
77	334.0
78	193.5
79	53.0
80	32.5
81	12.0
82	8.5
83	5.0
84	3.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	402284.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.388968987083752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80460974332111	8.17780473496336
2	8.769862056923346	1.9975937397460501
3	3.2324951981840404	1.1044436268904554
4	1.5125720272393923	0.6890654363583936
5	0.9036144578313252	0.5145618518260732
6	0.5609394098131657	0.3833112925197124
7	0.44307665444386246	0.3532330393453381
8	0.3121180373668587	0.28437621183044814
9	0.30557010651300853	0.31321156198108796
>10	7.565042779814912	23.510256435751856
>50	2.922559804435132	23.080211989539727
>100	1.6020604155753448	34.426176532002266
>500	0.056748734066701584	3.7282119099939344
>1k	0.008730574471800244	1.437541637251295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1943	0.48299211502321737	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1474	0.3664078113969236	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1221	0.30351691839595907	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1145	0.28462479243519506	No Hit
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	721	0.17922661602251147	No Hit
GAATTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT	700	0.17400642332282665	No Hit
GAATTAGGGTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC	658	0.16356603792345706	No Hit
GAATTAGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC	645	0.16033449006174744	No Hit
GAATTAGGGCACTAGCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATATT	627	0.1558600391763033	No Hit
GAATTAGGGGAACCGTGGCTATAGCGGAGCATGCTCCAGAGCACAGGACTGA	606	0.15063984647661852	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	604	0.1501426852671247	No Hit
GAATTAGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG	602	0.14964552405763093	No Hit
GAATTAGGGACTGAGAGTGGCATGCAAAATACATTTATTTGCTATTCTGTCT	591	0.1469111374054151	No Hit
GAATTAGGGGTCCTGTGGAAGACACTGAGGAAGCTGGACACTGGAGAGGTCT	589	0.14641397619592128	No Hit
GAATTAGGGGAACACATCCAGGTGAACAAGGCACCTAAGATGCGCCGACGAA	585	0.14541965377693372	No Hit
GAATTAGGGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGC	578	0.14367958954370544	No Hit
GAATTAGGGAAGGTTCTTCATAATTGCGGTGTAGAAGAAATTATGTCATAAA	569	0.1414423641009834	No Hit
GAATTAGGGCGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCT	562	0.13970229986775512	No Hit
GAATTAGGGGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGGTTTAAAAA	561	0.13945371926300823	No Hit
GAATTAGGGTCCTGATGTTCCTCTGGACACATCAATCCAGCCCCAGGGGCAG	553	0.13746507442503306	No Hit
GAATTAGGGGGATAGCTGTCAGCGTGCACCTAAAGTGGGACTAAGGGAAAGA	542	0.13473068777281721	No Hit
GAATTAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	541	0.13448210716807033	No Hit
GAATTAGGGCTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCCCC	540	0.13423352656332344	No Hit
GAATTAGGGGAAGGCCCCACCCCACCCATTGAGTCTTCCAAGAGTGGTGAGG	539	0.13398494595857652	No Hit
GAATTAGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA	521	0.12951049507313242	No Hit
GAATTAGGGTACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCCCT	519	0.1290133338636386	No Hit
GAATTAGGGTTATTGTTACCTAAAAACAAAGAAAGAAAGAAAGAAAGAAAGA	514	0.12777043083990414	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	513	0.12752185023515725	No Hit
GAATTAGGGAATCCTTAGCAATCAGAACACTTGCTTCACTAGATTTTGCCAA	513	0.12752185023515725	No Hit
GAATTAGGGTAAGAGATGAGAAACTTAAGTTCACCCAAGGGATGGTTTCAGG	505	0.1255332053971821	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	491	0.12205307693072555	No Hit
GAATTAGGGATTCTTTGTATTACACATGAAATATCGTTGCATATATGGACAG	472	0.11733004544053456	No Hit
GAATTAGGGGAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAG	468	0.11633572302154696	No Hit
GAATTAGGGAGTTGGTGAGGAGTCCCTATCCCAACTTGGCCCCAACATTGAG	466	0.11583856181205318	No Hit
GAATTAGGGATCCTCTGTGATTGTGGTGAGTTCAGGAAGATCAAGCCAAAGA	462	0.11484423939306561	No Hit
GAATTAGGGCGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAACA	461	0.1145956587883187	No Hit
GAATTAGGGGACGGCTTGCGAGCGCTATGGAGGCCTACGAGCAAGTCCAGAA	453	0.11260701395034353	No Hit
GAATTAGGGACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCAC	424	0.10539817641268359	No Hit
GAATTAGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGA	411	0.10216662855097394	No Hit
GAATTAGGGGGATCTATCACCTGTCACCATAATTGGCTGCTGCTTACCATCC	410	0.10191804794622704	No Hit
GAATTAGGGGGACTGATCAGTAAGATGGCCCATTATTGAATCGAGTCGAAAA	404	0.10042656431774567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.028338188941146057	0.0	0.0	0.0	0.0
7	0.029332511360133637	0.0	0.0	0.0	0.0
8	0.029332511360133637	0.0	0.0	0.0	0.0
9	0.030575414383868113	0.0	0.0	0.0	0.0
10	0.04524167006393493	0.0	0.0	0.0	0.0
11	0.08402024440445059	0.0	0.0	0.0	0.0
12	0.10316095096996152	0.0	0.0	0.0	0.0
13	0.11807578725477523	0.0	0.0	0.0	0.0
14	0.1357250101918048	0.0	0.0	0.0	0.0
15	0.1511370076861123	0.0	0.0	0.0	0.0
16	0.1620745542949757	0.0	0.0	0.0	0.0
17	0.17450358453232045	0.0	0.0	0.0	0.0
18	0.1871811953744121	0.0	0.0	0.0	0.0
19	0.20159887044973204	0.0	0.0	0.0	0.0
20	0.21154209463960785	0.0	0.0	0.0	0.0
21	0.22073957701524297	0.0	0.0	0.0	0.0
22	0.2344115102763222	0.0	0.0	0.0	0.0
23	0.2503206689801235	0.0	0.0	0.0	0.0
24	0.2672241501029124	0.0	0.0	0.0	0.0
25	0.2816418251782323	0.0	0.0	0.0	0.0
26	0.29556233904405843	0.0	0.0	0.0	0.0
27	0.3124658201668473	0.0	0.0	0.0	0.0
28	0.33235226854659894	0.0	0.0	0.0	0.0
29	0.34428413757444987	0.0	0.0	0.0	0.0
30	0.3609390380924919	0.0	0.0	0.0	0.0
31	0.3741138101440773	0.0	0.0	0.0	0.0
32	0.3860456791719283	0.0	0.0	0.0	0.0
33	0.3959889033618041	0.0	0.0	0.0	0.0
34	0.4079207723896551	0.0	0.0	0.0	0.0
35	0.4275586401646598	0.0	0.0	0.0	0.0
36	0.43949050919251076	0.0	0.0	0.0	0.0
37	0.4481908303586521	0.0	0.0	0.0	0.0
38	0.4581340545485279	0.0	0.0	0.0	0.0
39	0.4678286981336568	0.0	0.0	0.0	0.0
40	0.483986437442205	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	35	1.0173608E-7	46.000004	24
TCGCAAA	35	1.0173608E-7	46.000004	32
CAAACGA	35	1.0173608E-7	46.000004	45
AGATACG	35	1.0173608E-7	46.000004	37
TTTCGAC	35	1.0173608E-7	46.000004	44
ATTACTC	35	1.0173608E-7	46.000004	16
AACCACG	35	1.0173608E-7	46.000004	21
TGTATCG	35	1.0173608E-7	46.000004	23
CCGTCTA	35	1.0173608E-7	46.000004	44
AATGCCG	35	1.0173608E-7	46.000004	16
CTCGCAA	35	1.0173608E-7	46.000004	31
CATAATG	35	1.0173608E-7	46.000004	44
CGATGCA	35	1.0173608E-7	46.000004	29
CCGAAGG	35	1.0173608E-7	46.000004	32
GCGTGCT	35	1.0173608E-7	46.000004	12
CGGTAGT	70	0.0	46.000004	12
CCTATAT	35	1.0173608E-7	46.000004	37
CTTAGTA	35	1.0173608E-7	46.000004	29
TATCGCG	35	1.0173608E-7	46.000004	25
CCCGCTA	35	1.0173608E-7	46.000004	45
>>END_MODULE