FastQCFastQC Report
Fri 17 Jun 2016
SRR1527354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481083
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52461.0904563245843233No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT25410.5281832864599249No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24000.4988744146020541No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGA13620.28311123028666574No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT13590.28248763726841314No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCA12980.26980791256394426No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG10240.2128530835635431No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCA10070.20931938979344522No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC9970.20724074639926998No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC9850.20474637432625972No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG9300.19331383565829596No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT8600.1787633318990694No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGC8080.1679543862493582No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTAC6880.1430106655192555No Hit
AGGGTCGGGAGTTCCAGGACAGACATGGCTACATCAAGAAACCCTGTCTG6140.12762870440235885No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC6070.12617365402643618No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAG5910.12284782459575583No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGG5900.1226399602563383No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTA5780.12014558818332803No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCAC5710.11869053780740536No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT5670.11785908044973528No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG5530.11494897969788996No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5500.11432538667963739No Hit
AGGGTCGGGGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGT5460.11349392932196731No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC5440.11307820064313226No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC5370.1116231502672096No Hit
AGGGTCGGGAGCTGGCAAATTGCTCTCTTACCCTGTTCTTGCTGGTGCAG5230.10871304951536429No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTC5220.10850518517594678No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACA5210.10829732083652925No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT5080.10559508442410145No Hit
AGGGTCGGGGTGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTC5050.10497149140584888No Hit
AGGGTCGGGGTATGTATGCACACCTGTTCATCTGTGAAGGATGTGTGTGT4910.10206139065400356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT800.044.00000411
AACCGCA207.851911E-444.00000432
GTACCGC207.851911E-444.0000049
TACCCGT207.851911E-444.00000425
AAATCGC408.294592E-944.00000429
AAATCCG207.851911E-444.00000438
ACCGGCG207.851911E-444.00000429
CGTATCG207.851911E-444.0000046
CTACTCG207.851911E-444.00000435
GTCGAGT207.851911E-444.0000044
AATCACC207.851911E-444.00000432
GATCGAC207.851911E-444.0000049
CGACGGC408.294592E-944.00000444
CGACGCA207.851911E-444.00000418
TAGACGA207.851911E-444.00000415
CGTGATG207.851911E-444.00000438
ACCGCAG207.851911E-444.00000411
CGTTGTG207.851911E-444.00000416
CGTTGCG207.851911E-444.00000415
TCCCTCG207.851911E-444.00000442
Produced by FastQC (version 0.11.5)