##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481083
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.697233117778012	31.0	30.0	33.0	25.0	33.0
2	31.00827300070882	31.0	31.0	33.0	28.0	34.0
3	31.97507290841705	31.0	31.0	34.0	31.0	34.0
4	36.238590846070224	37.0	37.0	37.0	35.0	37.0
5	33.871814219168	35.0	33.0	35.0	32.0	35.0
6	35.30905893577616	35.0	35.0	37.0	35.0	37.0
7	36.066277544623276	37.0	35.0	37.0	35.0	37.0
8	36.55292745742419	37.0	37.0	37.0	35.0	37.0
9	38.51437485839242	39.0	39.0	39.0	37.0	39.0
10	36.00936220984737	38.0	35.0	39.0	31.0	39.0
11	36.387812498051275	37.0	35.0	39.0	31.0	39.0
12	36.23534192644512	37.0	35.0	39.0	32.0	39.0
13	36.58824776597801	38.0	35.0	39.0	33.0	39.0
14	37.37059093753053	39.0	37.0	40.0	33.0	40.0
15	37.445199684877664	39.0	37.0	40.0	33.0	40.0
16	37.5767965195195	39.0	37.0	40.0	33.0	40.0
17	37.51515434966524	39.0	37.0	40.0	33.0	40.0
18	37.3484055765845	39.0	36.0	40.0	33.0	40.0
19	37.27848417009123	39.0	36.0	40.0	33.0	40.0
20	37.05879858569104	38.0	36.0	40.0	33.0	40.0
21	36.97549695166946	38.0	36.0	40.0	33.0	40.0
22	36.83368566338864	38.0	35.0	40.0	32.0	40.0
23	36.591488371029534	38.0	35.0	40.0	32.0	40.0
24	36.5089204981261	38.0	35.0	40.0	32.0	40.0
25	36.31886389666648	38.0	35.0	39.0	31.0	40.0
26	35.73967693724368	38.0	34.0	39.0	30.0	40.0
27	35.61152233606259	37.0	34.0	39.0	30.0	40.0
28	35.53762656339966	37.0	34.0	38.0	30.0	40.0
29	35.26373411656616	37.0	34.0	38.0	30.0	40.0
30	34.97643026255345	36.0	34.0	38.0	30.0	40.0
31	34.91220849624701	36.0	33.0	38.0	30.0	39.0
32	34.83829401579353	36.0	33.0	38.0	29.0	39.0
33	34.325929205563284	36.0	33.0	38.0	29.0	39.0
34	33.872855619508485	35.0	32.0	38.0	27.0	39.0
35	33.687315910144406	35.0	32.0	38.0	27.0	39.0
36	33.461074284478975	35.0	32.0	38.0	27.0	39.0
37	33.317913956635344	34.0	31.0	37.0	27.0	38.0
38	32.98968992876489	34.0	31.0	37.0	26.0	38.0
39	32.21430189800928	34.0	30.0	37.0	24.0	38.0
40	31.883454622175382	33.0	30.0	36.0	24.0	38.0
41	31.687789840838274	33.0	30.0	36.0	24.0	38.0
42	31.410864653292673	33.0	29.0	36.0	23.0	38.0
43	30.811082495120385	32.0	29.0	35.0	23.0	38.0
44	30.40602349282764	32.0	28.0	35.0	23.0	37.0
45	29.84808026889331	31.0	27.0	34.0	21.0	37.0
46	29.71740843056188	31.0	27.0	34.0	21.0	37.0
47	29.199849090489582	31.0	26.0	34.0	20.0	36.0
48	28.691986621851115	30.0	26.0	34.0	20.0	36.0
49	28.23646855116477	30.0	26.0	33.0	19.0	35.0
50	31.962513745029444	34.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	6.0
14	7.0
15	19.0
16	46.0
17	84.0
18	141.0
19	224.0
20	406.0
21	547.0
22	804.0
23	1203.0
24	1917.0
25	2843.0
26	4150.0
27	5727.0
28	7947.0
29	10611.0
30	14739.0
31	20914.0
32	33395.0
33	50072.0
34	70850.0
35	124639.0
36	114769.0
37	14723.0
38	295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.08290253448989	0.7260701375854063	0.12492646798993107	0.06610085993477216
8	99.2847388080643	0.35544802040396356	0.10538722008468393	0.2544259514470476
9	95.87493218425927	2.5161978286491102	0.5121777323247756	1.096692254766849
10	43.426394198090556	46.33254552748694	4.113011684054518	6.128048590367982
11	35.33132536381456	20.85211907300819	29.844330396210218	13.97222516696703
12	28.166241584092553	20.21459914401465	25.50142075275992	26.11773851913287
13	25.11125938767323	19.344686883552317	31.5405034058572	24.003550322917253
14	26.656730751242506	19.403720355946895	28.79690198988532	25.142646902925275
15	27.980826593332125	22.244186554087342	28.49196500395982	21.283021848620717
16	31.47939129006845	24.945383644818044	23.652675317980474	19.92254974713303
17	30.21952552885885	22.430017273526605	24.777013529889853	22.573443667724696
18	27.897057264546866	25.094422376180407	25.217893793794417	21.790626565478306
19	28.903952124685343	23.48160296663985	25.929621291960014	21.684823616714787
20	27.58297424768699	26.639685875410272	24.093763446224457	21.68357643067828
21	27.92096166357988	26.0551713529682	26.68832613083397	19.335540852617946
22	28.004730992365147	22.43209591692078	28.164786533716637	21.398386556997444
23	25.80427909529125	24.495149485639693	28.256662571739184	21.44390884732988
24	26.7444495024767	23.06878438855665	26.44366980333955	23.743096305627095
25	24.31991984751072	27.37157621449937	25.270691336006468	23.037812601983443
26	23.643321422706684	27.081189732333087	25.26778123525462	24.0077076097056
27	23.813354452350218	26.519124558548107	26.962707058865103	22.70481393023657
28	23.44003009875635	25.86913276918952	25.233899347929565	25.456937784124566
29	25.394786346638732	26.161182166071136	24.651255604542254	23.792775882747883
30	26.8606456682111	24.041173768351822	27.63660324725671	21.461577316180367
31	26.504781919128302	25.16821421667363	25.256348696586663	23.07065516761141
32	30.245716435625454	23.760764774477586	24.77410342913801	21.21941536075895
33	27.69833895606372	25.215607286060827	24.962012791971446	22.124040965904012
34	24.507413481665328	26.347636478528653	28.838682722108246	20.306267317697777
35	23.97528077275647	28.500487441875933	27.300070881739742	20.224160903627855
36	26.36883864114924	26.780202168856515	24.030988415720365	22.81997077427388
37	24.15882498446214	28.456004473240586	25.0836134305307	22.301557111766577
38	27.296121459290806	27.51999135284348	24.3718859323651	20.812001255500608
39	26.837364862196335	26.637815096355517	23.99128632689162	22.53353371455653
40	26.223125739217558	25.291062041269385	24.03369065213279	24.452121567380264
41	21.94985064947213	28.669689014161797	24.955776861788923	24.42468347457715
42	23.21158718973649	24.97427678799708	25.94022237327031	25.873913648996123
43	22.687769054404335	24.736479983703436	25.504330853511764	27.07142010838047
44	23.857837420985568	25.16800635233421	25.938351594215554	25.03580463246467
45	24.84768740529181	25.11001220163672	26.26532220011931	23.776978192952154
46	25.256556560926075	27.562187813745236	25.598077670589063	21.583177954739618
47	22.548915675673427	26.84942099388255	26.935892559080244	23.66577077136378
48	24.7728562431015	24.897782711091434	25.007535082303882	25.32182596350318
49	26.271973858980672	25.73630745630172	24.989450884774563	23.002267799943045
50	24.371470203686265	24.729828324842078	25.95061559024118	24.948085881230476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	1.5
12	3.0
13	272.0
14	541.0
15	993.5
16	1446.0
17	1573.5
18	1701.0
19	1556.5
20	1412.0
21	1352.0
22	1292.0
23	1434.5
24	1577.0
25	1715.5
26	1854.0
27	2433.5
28	3013.0
29	3227.0
30	3441.0
31	4371.5
32	5302.0
33	6532.0
34	7762.0
35	8239.0
36	8716.0
37	10255.0
38	11794.0
39	13573.5
40	15353.0
41	16520.5
42	17688.0
43	17809.5
44	17931.0
45	19188.0
46	20445.0
47	21247.0
48	22049.0
49	24781.5
50	27514.0
51	28443.5
52	29373.0
53	29694.5
54	30016.0
55	32069.5
56	34123.0
57	33390.5
58	32658.0
59	35195.5
60	37733.0
61	37092.5
62	36452.0
63	34383.5
64	32315.0
65	31139.0
66	29963.0
67	24374.0
68	18785.0
69	14849.5
70	10914.0
71	8692.5
72	6471.0
73	5235.5
74	4000.0
75	4143.5
76	4287.0
77	3213.0
78	2139.0
79	1324.5
80	510.0
81	497.0
82	484.0
83	246.5
84	9.0
85	6.5
86	4.0
87	3.5
88	3.0
89	3.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	481083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.61625540707113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62492902316292	7.603885400232392
2	10.453664362774852	2.2195754163003056
3	3.594854424059679	1.1449167815117143
4	1.630998766471521	0.6926039789391851
5	0.8341002095040432	0.442751042959323
6	0.5188651537994635	0.33050429967386086
7	0.33873083625398936	0.2517237150346198
8	0.272159458030662	0.23114514543228507
9	0.2525796409061539	0.24133049806374368
>10	5.781919996867229	16.99124683266713
>50	2.580619897010162	19.195648152189953
>100	2.0558807980733462	44.22500899013268
>500	0.04503357938636853	3.054566467740494
>1k	0.01370587198715564	2.2846369545379903
>5k	0.001957981712450806	1.0904563245843233
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5246	1.0904563245843233	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2541	0.5281832864599249	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2400	0.4988744146020541	No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGA	1362	0.28311123028666574	No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT	1359	0.28248763726841314	No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCA	1298	0.26980791256394426	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1024	0.2128530835635431	No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCA	1007	0.20931938979344522	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	997	0.20724074639926998	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	985	0.20474637432625972	No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAG	930	0.19331383565829596	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	860	0.1787633318990694	No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGC	808	0.1679543862493582	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTAC	688	0.1430106655192555	No Hit
AGGGTCGGGAGTTCCAGGACAGACATGGCTACATCAAGAAACCCTGTCTG	614	0.12762870440235885	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	607	0.12617365402643618	No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAG	591	0.12284782459575583	No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGG	590	0.1226399602563383	No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTA	578	0.12014558818332803	No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCAC	571	0.11869053780740536	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT	567	0.11785908044973528	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	553	0.11494897969788996	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	550	0.11432538667963739	No Hit
AGGGTCGGGGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGT	546	0.11349392932196731	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	544	0.11307820064313226	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	537	0.1116231502672096	No Hit
AGGGTCGGGAGCTGGCAAATTGCTCTCTTACCCTGTTCTTGCTGGTGCAG	523	0.10871304951536429	No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTC	522	0.10850518517594678	No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACA	521	0.10829732083652925	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	508	0.10559508442410145	No Hit
AGGGTCGGGGTGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTC	505	0.10497149140584888	No Hit
AGGGTCGGGGTATGTATGCACACCTGTTCATCTGTGAAGGATGTGTGTGT	491	0.10206139065400356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0010393216970876128	0.0	0.0	0.0	0.0
8	0.0012471860365051352	0.0	0.0	0.0	0.0
9	0.0018707790547577029	0.0	0.0	0.0	0.0
10	0.012263996025633831	0.0	0.0	0.0	0.0
11	0.018084197529324463	0.0	0.0	0.0	0.0
12	0.02369653469359757	0.0	0.0	0.0	0.0
13	0.028685278839618113	0.0	0.0	0.0	0.0
14	0.0378313097739891	0.0	0.0	0.0	0.0
15	0.047600933726612664	0.0	0.0	0.0	0.0
16	0.059657065412828975	0.0	0.0	0.0	0.0
17	0.06672445295302473	0.0	0.0	0.0	0.0
18	0.07358397615380298	0.0	0.0	0.0	0.0
19	0.08044349935458123	0.0	0.0	0.0	0.0
20	0.087510886894777	0.0	0.0	0.0	0.0
21	0.09437041009555523	0.0	0.0	0.0	0.0
22	0.09977488292041083	0.0	0.0	0.0	0.0
23	0.1033085766905087	0.0	0.0	0.0	0.0
24	0.11037596423070448	0.0	0.0	0.0	0.0
25	0.11681975875264768	0.0	0.0	0.0	0.0
26	0.12367928195342591	0.0	0.0	0.0	0.0
27	0.13282531288779692	0.0	0.0	0.0	0.0
28	0.13947697174915763	0.0	0.0	0.0	0.0
29	0.14987018872003377	0.0	0.0	0.0	0.0
30	0.1575611692784821	0.0	0.0	0.0	0.0
31	0.1642128281398428	0.0	0.0	0.0	0.0
32	0.1725274017165437	0.0	0.0	0.0	0.0
33	0.18001051793557452	0.0	0.0	0.0	0.0
34	0.1862464481181002	0.0	0.0	0.0	0.0
35	0.19767898678606394	0.0	0.0	0.0	0.0
36	0.2111901688482029	0.0	0.0	0.0	0.0
37	0.21825755638839867	0.0	0.0	0.0	0.0
38	0.22719572298335214	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	80	0.0	44.000004	11
AACCGCA	20	7.851911E-4	44.000004	32
GTACCGC	20	7.851911E-4	44.000004	9
TACCCGT	20	7.851911E-4	44.000004	25
AAATCGC	40	8.294592E-9	44.000004	29
AAATCCG	20	7.851911E-4	44.000004	38
ACCGGCG	20	7.851911E-4	44.000004	29
CGTATCG	20	7.851911E-4	44.000004	6
CTACTCG	20	7.851911E-4	44.000004	35
GTCGAGT	20	7.851911E-4	44.000004	4
AATCACC	20	7.851911E-4	44.000004	32
GATCGAC	20	7.851911E-4	44.000004	9
CGACGGC	40	8.294592E-9	44.000004	44
CGACGCA	20	7.851911E-4	44.000004	18
TAGACGA	20	7.851911E-4	44.000004	15
CGTGATG	20	7.851911E-4	44.000004	38
ACCGCAG	20	7.851911E-4	44.000004	11
CGTTGTG	20	7.851911E-4	44.000004	16
CGTTGCG	20	7.851911E-4	44.000004	15
TCCCTCG	20	7.851911E-4	44.000004	42
>>END_MODULE