SRR1527353_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372265
Sequences flagged as poor quality	0
Sequence length	52
%GC	53

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4149	1.1145286287993768	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2023	0.5434300834083248	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1915	0.5144184922031348	No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA	1074	0.2885041569849435	No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATG	1057	0.28393751762857106	No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA	1051	0.28232576256161607	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	975	0.26191019838018614	No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCAAC	893	0.23988287913180129	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	838	0.22510845768471385	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	777	0.2087222811706714	No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGC	748	0.20093213168038895	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	690	0.18535183269982405	No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGCCA	619	0.16627939774085665	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTACTC	551	0.14801284031536674	No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAGCC	508	0.1364619290021893	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	497	0.1335070447127718	No Hit
AGGGTCGGGAGTTCCAGGACAGACATGGCTACATCAAGAAACCCTGTCTGGG	480	0.12894040535639933	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	453	0.12168750755510188	No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTATC	453	0.12168750755510188	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT	453	0.12168750755510188	No Hit
AGGGTCGGGGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGT	444	0.11926987495466938	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	438	0.11765811988771441	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	428	0.11497186144278941	No Hit
AGGGTCGGGGTATGTATGCACACCTGTTCATCTGTGAAGGATGTGTGTGTGT	428	0.11497186144278941	No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCACCT	419	0.11255422884235693	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	416	0.11174835130887942	No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGGAG	412	0.11067384793090942	No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTCAG	400	0.10745033779699946	No Hit
AGGGTCGGGGAACAATGCAGGGCCTCCTGGGGAAGGAGATGTTGTGGGAGCT	400	0.10745033779699946	No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACATT	397	0.10664446026352195	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	380	0.10207782090714947	No Hit
AGGGTCGGGGTACGGGGACGACAGCGGGCAAGGTTTATACGTGCCTTTTTGT	375	0.10073469168468697	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	35	1.0170697E-7	46.000004	11
GTCGCAA	35	1.0170697E-7	46.000004	4
TCGCAAG	35	1.0170697E-7	46.000004	5
CAAACGA	35	1.0170697E-7	46.000004	26
CTCGTAC	35	1.0170697E-7	46.000004	37
GGTCGCA	35	1.0170697E-7	46.000004	3
ACGTTTA	35	1.0170697E-7	46.000004	37
CACGGAT	35	1.0170697E-7	46.000004	39
CGAAACC	35	1.0170697E-7	46.000004	31
ACGCACG	35	1.0170697E-7	46.000004	33
CGTCTCA	35	1.0170697E-7	46.000004	39
TCGATCC	35	1.0170697E-7	46.000004	13
TACATCG	35	1.0170697E-7	46.000004	26
CGCCTAG	35	1.0170697E-7	46.000004	30
TAGCACG	35	1.0170697E-7	46.000004	36
CGTAGGT	35	1.0170697E-7	46.000004	43
GATATCG	35	1.0170697E-7	46.000004	9
CATCGAA	35	1.0170697E-7	46.000004	28
GAACCGA	35	1.0170697E-7	46.000004	8
GACGCAC	35	1.0170697E-7	46.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content