##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372265
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193238687494123	31.0	31.0	33.0	30.0	33.0
2	31.91040790834486	33.0	31.0	34.0	30.0	34.0
3	32.63002431063892	34.0	31.0	34.0	31.0	34.0
4	36.44996172081716	37.0	37.0	37.0	35.0	37.0
5	34.10359555692853	35.0	35.0	35.0	32.0	35.0
6	35.587567995916885	36.0	35.0	37.0	35.0	37.0
7	36.26178931675017	37.0	35.0	37.0	35.0	37.0
8	36.64121257706204	37.0	37.0	37.0	35.0	37.0
9	38.64058130632748	39.0	39.0	39.0	39.0	39.0
10	37.09548037016641	39.0	37.0	39.0	34.0	39.0
11	37.47946221105933	39.0	37.0	39.0	35.0	39.0
12	37.37087289968168	39.0	37.0	39.0	35.0	39.0
13	37.426392489221385	39.0	37.0	39.0	35.0	39.0
14	38.16725988207325	40.0	38.0	40.0	35.0	40.0
15	38.17048607846561	40.0	38.0	40.0	35.0	40.0
16	38.23127342081582	40.0	38.0	40.0	35.0	40.0
17	38.17883228345399	40.0	38.0	40.0	35.0	40.0
18	37.9932789813708	40.0	38.0	40.0	34.0	40.0
19	37.92862074060145	40.0	38.0	40.0	34.0	40.0
20	37.747282178018345	40.0	37.0	40.0	33.0	40.0
21	37.768877009657096	40.0	37.0	40.0	33.0	40.0
22	37.779216418411615	39.0	37.0	40.0	34.0	40.0
23	37.64769720494809	39.0	37.0	40.0	34.0	40.0
24	37.691007749855615	39.0	37.0	40.0	34.0	40.0
25	37.61496245953823	39.0	37.0	40.0	34.0	40.0
26	37.60155802989806	39.0	37.0	40.0	34.0	40.0
27	37.55660080856379	39.0	36.0	40.0	34.0	40.0
28	37.54038923884867	39.0	36.0	40.0	34.0	40.0
29	37.451761513975256	39.0	36.0	40.0	33.0	40.0
30	37.278452177884034	39.0	36.0	40.0	33.0	40.0
31	37.26624044699341	39.0	36.0	40.0	33.0	40.0
32	37.18966865002082	39.0	35.0	40.0	33.0	40.0
33	36.91939612910158	39.0	35.0	40.0	32.0	40.0
34	36.66081151867621	38.0	35.0	40.0	31.0	40.0
35	36.576218553987076	38.0	35.0	40.0	31.0	40.0
36	36.59976360925685	38.0	35.0	40.0	31.0	40.0
37	36.44852725880757	38.0	35.0	40.0	31.0	40.0
38	36.60188843968678	38.0	35.0	40.0	32.0	40.0
39	36.63854780868467	38.0	35.0	40.0	32.0	40.0
40	36.55588089130055	38.0	35.0	40.0	31.0	40.0
41	36.42795589163634	38.0	35.0	40.0	31.0	40.0
42	36.32023692799484	38.0	35.0	40.0	31.0	40.0
43	36.27535492189703	38.0	35.0	40.0	31.0	40.0
44	36.194826266235076	38.0	35.0	40.0	31.0	40.0
45	35.9676574483231	38.0	35.0	40.0	31.0	40.0
46	35.82363370179845	37.0	34.0	40.0	30.0	40.0
47	35.69477119793695	37.0	34.0	40.0	30.0	40.0
48	35.56742643009684	37.0	34.0	40.0	30.0	40.0
49	35.394522719030796	37.0	34.0	40.0	30.0	40.0
50	35.871758021839284	37.0	35.0	40.0	31.0	40.0
51	35.943266221643185	37.0	35.0	40.0	31.0	40.0
52	35.633862973956724	37.0	34.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	4.0
15	15.0
16	15.0
17	32.0
18	61.0
19	99.0
20	149.0
21	200.0
22	265.0
23	426.0
24	587.0
25	878.0
26	1253.0
27	1670.0
28	2545.0
29	3520.0
30	4685.0
31	6667.0
32	8816.0
33	14491.0
34	28406.0
35	27862.0
36	48065.0
37	80454.0
38	131979.0
39	9115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.08908976132594	0.7134702429720763	0.13592467731320432	0.061515318388782186
8	99.28088861429359	0.35566061810806815	0.10476407935207446	0.25868668824627616
9	95.92091655138141	2.532873087719769	0.47439324137375255	1.0718171195250694
10	43.65546049185392	45.96806038708984	4.262554900406968	6.1139242206492685
11	35.24290491988234	20.742213208332775	29.920621062952467	14.094260808832418
12	28.335728580446723	20.071723100479495	25.514351335742013	26.078196983331765
13	25.239547096826186	19.421917182652145	31.401286717795116	23.937249002726553
14	26.837064994023073	19.222059554349723	28.81227082857642	25.128604623050784
15	28.00182665574255	22.22314748901992	28.471921883604423	21.30310397163311
16	31.324728352114757	25.05392663828187	23.60146669711093	20.019878312492445
17	30.191664540045398	22.234429774488603	24.949431184774287	22.62447450069171
18	28.177776583885134	24.839831840221347	25.09905578015661	21.883335795736908
19	28.897693847125034	23.36588183149101	26.136220165742145	21.600204155641816
20	27.562623399997317	26.53110015714612	24.178475011080817	21.727801431775752
21	28.16085315568211	25.836165097444024	26.60550951607054	19.397472230803324
22	28.195237263777145	22.330060575127934	28.271527003613016	21.2031751574819
23	25.877533477495867	24.08848535317583	28.32041690731065	21.71356426201765
24	26.759432124964743	22.824063503149638	26.69791680657596	23.718587565309658
25	24.452204746618673	27.23758612816139	25.346997434623187	22.96321169059675
26	23.710797415819375	26.890252911232587	25.555988341638347	23.842961331309688
27	23.942890145460897	26.366163888627725	26.91550374061488	22.775442225296498
28	23.486226209823645	25.82595731535331	25.18313566948276	25.50468080534028
29	25.425704807059486	26.001907243495896	24.84117496944381	23.73121298000081
30	26.940485944152687	23.876002310182262	27.79068674197144	21.392825003693606
31	26.61464279478329	25.226653056290544	25.117053711737608	23.04165043718856
32	30.19193316588989	23.632895920916553	24.82183390864035	21.353337004553207
33	27.706338226800803	24.974950640001076	25.207043369642594	22.11166776355553
34	24.75628920258418	26.284233006057512	28.690314695176824	20.269163096181483
35	23.975125246799994	28.56325467073187	27.248868413630074	20.21275166883806
36	26.70543833022175	26.55876861912885	24.041744456234134	22.69404859441527
37	24.254764750916685	28.37521657958712	25.150363316454676	22.219655353041517
38	27.32569540515493	27.53011967281372	24.453279249996644	20.690905672034706
39	26.979705317448595	26.44433400937504	24.03073079660994	22.545229876566424
40	26.178394423327468	25.362040481914764	24.049265979879923	24.410299114877844
41	21.989711630155938	28.663720736572067	24.98623292546976	24.360334707802238
42	23.590184411642245	24.806253609659784	25.84341799524532	25.76014398345265
43	22.82728701328355	24.29801351187998	25.7260285012021	27.148670973634374
44	23.87439055511531	24.89570601587579	26.211166776355554	25.018736652653352
45	24.890333498985935	25.017930775119872	26.47549460733617	23.616241118558015
46	25.296495775858595	27.362765771694896	25.60514687118048	21.735591581266036
47	22.488549823378506	26.61598592400575	27.022148200878405	23.873316051737337
48	24.84090634359932	24.644540851275302	25.07219319570736	25.442359609418023
49	26.347897331202237	25.496890655850002	25.058761903482736	23.09645010946503
50	24.409224611499873	24.471277181577637	26.178394423327468	24.94110378359502
51	22.651874336829948	25.501994546895357	26.732300914670997	25.113830201603697
52	23.249566840825757	24.869380683115523	27.143567082589016	24.737485393469704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	1.0
12	2.0
13	211.5
14	730.5
15	1040.0
16	1147.0
17	1254.0
18	1159.5
19	1065.0
20	1017.0
21	969.0
22	1000.0
23	1031.0
24	1000.5
25	970.0
26	1534.5
27	2099.0
28	2428.0
29	2757.0
30	3070.5
31	3384.0
32	3969.5
33	4555.0
34	5044.5
35	5534.0
36	6823.0
37	8112.0
38	8694.0
39	10244.5
40	11213.0
41	12393.0
42	13573.0
43	13095.0
44	12617.0
45	14358.5
46	16100.0
47	16877.0
48	17654.0
49	18171.5
50	18689.0
51	20819.5
52	22950.0
53	22135.5
54	21321.0
55	24417.0
56	27513.0
57	26233.5
58	24954.0
59	24920.5
60	24887.0
61	27008.0
62	29129.0
63	26982.5
64	24049.5
65	23263.0
66	18819.5
67	14376.0
68	12026.5
69	9677.0
70	8079.0
71	6481.0
72	4727.5
73	2974.0
74	3211.5
75	3449.0
76	3120.0
77	2791.0
78	1822.0
79	853.0
80	502.5
81	152.0
82	232.5
83	313.0
84	164.0
85	15.0
86	8.5
87	2.0
88	4.0
89	5.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	372265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.020724483902596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.46374437926208	6.085718506977556
2	9.886542985616867	1.7836756074301907
3	3.427533426640064	0.9275650410325977
4	1.3996009648313035	0.5050165876458974
5	0.8278490813257497	0.3733899238445731
6	0.51517227003365	0.27883362658321353
7	0.4407254102021977	0.27829637489422854
8	0.40499091748310057	0.2922649188078385
9	0.33054405765164824	0.26835721864800616
>10	9.61555641583038	22.903308127274926
>50	3.2041928471457073	19.93660430069977
>100	2.4388791280783777	41.56716317676924
>500	0.02680086953932283	1.7726619478059984
>1k	0.017867246359548554	3.027144641585967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4149	1.1145286287993768	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2023	0.5434300834083248	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1915	0.5144184922031348	No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA	1074	0.2885041569849435	No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATG	1057	0.28393751762857106	No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA	1051	0.28232576256161607	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	975	0.26191019838018614	No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCAAC	893	0.23988287913180129	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	838	0.22510845768471385	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	777	0.2087222811706714	No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGC	748	0.20093213168038895	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	690	0.18535183269982405	No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGCCA	619	0.16627939774085665	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTACTC	551	0.14801284031536674	No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAGCC	508	0.1364619290021893	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	497	0.1335070447127718	No Hit
AGGGTCGGGAGTTCCAGGACAGACATGGCTACATCAAGAAACCCTGTCTGGG	480	0.12894040535639933	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	453	0.12168750755510188	No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTATC	453	0.12168750755510188	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT	453	0.12168750755510188	No Hit
AGGGTCGGGGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGT	444	0.11926987495466938	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	438	0.11765811988771441	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	428	0.11497186144278941	No Hit
AGGGTCGGGGTATGTATGCACACCTGTTCATCTGTGAAGGATGTGTGTGTGT	428	0.11497186144278941	No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCACCT	419	0.11255422884235693	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	416	0.11174835130887942	No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGGAG	412	0.11067384793090942	No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTCAG	400	0.10745033779699946	No Hit
AGGGTCGGGGAACAATGCAGGGCCTCCTGGGGAAGGAGATGTTGTGGGAGCT	400	0.10745033779699946	No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACATT	397	0.10664446026352195	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	380	0.10207782090714947	No Hit
AGGGTCGGGGTACGGGGACGACAGCGGGCAAGGTTTATACGTGCCTTTTTGT	375	0.10073469168468697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0010745033779699946	0.0	0.0	0.0	0.0
8	0.0018803809114474905	0.0	0.0	0.0	0.0
9	0.002686258444924986	0.0	0.0	0.0	0.0
10	0.01692342820302741	0.0	0.0	0.0	0.0
11	0.02337044847084738	0.0	0.0	0.0	0.0
12	0.03116059796112984	0.0	0.0	0.0	0.0
13	0.0384134957624273	0.0	0.0	0.0	0.0
14	0.04378601265227727	0.0	0.0	0.0	0.0
15	0.05641142734342471	0.0	0.0	0.0	0.0
16	0.06661920943413965	0.0	0.0	0.0	0.0
17	0.07414073307992962	0.0	0.0	0.0	0.0
18	0.08031912750325709	0.0	0.0	0.0	0.0
19	0.08596027023759956	0.0	0.0	0.0	0.0
20	0.09133278712744954	0.0	0.0	0.0	0.0
21	0.09858568492874699	0.0	0.0	0.0	0.0
22	0.10315232428511947	0.0	0.0	0.0	0.0
23	0.11067384793090944	0.0	0.0	0.0	0.0
24	0.1171208681987294	0.0	0.0	0.0	0.0
25	0.12329926262205687	0.0	0.0	0.0	0.0
26	0.12840315366741434	0.0	0.0	0.0	0.0
27	0.1343129222462493	0.0	0.0	0.0	0.0
28	0.14210307173653178	0.0	0.0	0.0	0.0
29	0.14881871784884423	0.0	0.0	0.0	0.0
30	0.1571461190281117	0.0	0.0	0.0	0.0
31	0.16439901682940916	0.0	0.0	0.0	0.0
32	0.1740695472311391	0.0	0.0	0.0	0.0
33	0.1799793158099741	0.0	0.0	0.0	0.0
34	0.18535183269982405	0.0	0.0	0.0	0.0
35	0.19743999570198648	0.0	0.0	0.0	0.0
36	0.20576739688125395	0.0	0.0	0.0	0.0
37	0.2151693014384914	0.0	0.0	0.0	0.0
38	0.22430258015123636	0.0	0.0	0.0	0.0
39	0.2350476139309363	0.0	0.0	0.0	0.0
40	0.24149463419875628	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	35	1.0170697E-7	46.000004	11
GTCGCAA	35	1.0170697E-7	46.000004	4
TCGCAAG	35	1.0170697E-7	46.000004	5
CAAACGA	35	1.0170697E-7	46.000004	26
CTCGTAC	35	1.0170697E-7	46.000004	37
GGTCGCA	35	1.0170697E-7	46.000004	3
ACGTTTA	35	1.0170697E-7	46.000004	37
CACGGAT	35	1.0170697E-7	46.000004	39
CGAAACC	35	1.0170697E-7	46.000004	31
ACGCACG	35	1.0170697E-7	46.000004	33
CGTCTCA	35	1.0170697E-7	46.000004	39
TCGATCC	35	1.0170697E-7	46.000004	13
TACATCG	35	1.0170697E-7	46.000004	26
CGCCTAG	35	1.0170697E-7	46.000004	30
TAGCACG	35	1.0170697E-7	46.000004	36
CGTAGGT	35	1.0170697E-7	46.000004	43
GATATCG	35	1.0170697E-7	46.000004	9
CATCGAA	35	1.0170697E-7	46.000004	28
GAACCGA	35	1.0170697E-7	46.000004	8
GACGCAC	35	1.0170697E-7	46.000004	32
>>END_MODULE