SRR1527352_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489245
Sequences flagged as poor quality	0
Sequence length	52
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5274	1.0779875113695592	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2591	0.5295915134544043	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2456	0.5019979764739547	No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATG	1523	0.3112959764535151	No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA	1500	0.3065948553383274	No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA	1188	0.2428231254279553	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1128	0.23055933121442224	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1014	0.20725812220870934	No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCAAC	1005	0.2054185530766794	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	974	0.19908225939968727	No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGCCA	850	0.17373708469171886	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	839	0.1714887224192378	No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGC	799	0.16331285961021574	No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAGCC	655	0.13387975349773631	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTACTC	641	0.1310182015145786	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	610	0.12468190783758648	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	605	0.12365992498645872	No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGGAG	592	0.12100276957352657	No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTATC	586	0.11977639015217324	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	581	0.11875440730104549	No Hit
AGGGTCGGGGAACAATGCAGGGCCTCCTGGGGAAGGAGATGTTGTGGGAGCT	576	0.11773242444991774	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT	576	0.11773242444991774	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	571	0.11671044159878997	No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCACCT	571	0.11671044159878997	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	556	0.1136444930454067	No Hit
AGGGTCGGGGTACGGGGACGACAGCGGGCAAGGTTTATACGTGCCTTTTTGT	548	0.11200932048360229	No Hit
AGGGTCGGGAGCTGGCAAATTGCTCTCTTACCCTGTTCTTGCTGGTGCAGTC	530	0.10833018221954235	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	526	0.10751259593864014	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	515	0.10526423366615908	No Hit
AGGGTCGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC	513	0.10485544052570797	No Hit
AGGGTCGGGGCGCGGCCGCCGACTGGGCAGGCACCACTTGGCCTAGCCCGCT	509	0.10403785424480576	No Hit
AGGGTCGGGGTGAGGGGCCCAGATGCGTGTGCACAGGTACACAAACTCTACA	505	0.10322026796390357	No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTCAG	501	0.10240268168300135	No Hit
AGGGTCGGGGAGCCGACTCCACAGGAGCCAAGGGCACGCGACTCAAGGAGGG	500	0.10219828511277582	No Hit
AGGGTCGGGAGCCAGGGCTACACAGAAAAACTCTTATTTTAAAAATAACAGC	498	0.1017894919723247	No Hit
AGGGTCGGGGCCAGGCGTGGTGGCGCACGCCTTTAGTCCCAGCACTCGGGAG	496	0.1013806988318736	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	492	0.1005631125509714	No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACATT	491	0.10035871598074585	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	35	1.01796104E-7	46.000004	6
CGGAAAC	35	1.01796104E-7	46.000004	22
TTGGCGA	35	1.01796104E-7	46.000004	13
GTACCGT	55	1.8189894E-12	46.000004	36
GCGACAA	35	1.01796104E-7	46.000004	21
CGACGGC	35	1.01796104E-7	46.000004	44
GCGAAAT	35	1.01796104E-7	46.000004	16
CGTGATC	55	1.8189894E-12	46.000004	18
TGGCGAA	35	1.01796104E-7	46.000004	14
CCGTGAT	55	1.8189894E-12	46.000004	17
TTCGTAA	35	1.01796104E-7	46.000004	19
ATAGACG	55	1.8189894E-12	46.000004	27
CGACAAT	35	1.01796104E-7	46.000004	22
AATGCGC	55	1.8189894E-12	46.000004	39
CCCGTCG	35	1.01796104E-7	46.000004	35
TCAATAT	70	0.0	46.000004	28
TCGGAAT	35	1.01796104E-7	46.000004	5
GTTCGCA	55	1.8189894E-12	46.000004	38
CGTAGCT	35	1.01796104E-7	46.000004	38
TGCGAAT	35	1.01796104E-7	46.000004	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content