##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489245
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.3621232715715	31.0	30.0	31.0	25.0	33.0
2	30.702486484276793	31.0	31.0	33.0	28.0	34.0
3	31.483792373963965	31.0	31.0	34.0	30.0	34.0
4	35.832329405512574	37.0	35.0	37.0	35.0	37.0
5	33.23450009708837	35.0	33.0	35.0	28.0	35.0
6	34.99955441547691	35.0	35.0	36.0	33.0	37.0
7	35.842751586628374	37.0	35.0	37.0	35.0	37.0
8	36.37232061646006	37.0	35.0	37.0	35.0	37.0
9	38.46300115484062	39.0	39.0	39.0	37.0	39.0
10	36.07362977649235	39.0	35.0	39.0	31.0	39.0
11	36.56004455845231	38.0	35.0	39.0	32.0	39.0
12	36.46720559229016	38.0	35.0	39.0	32.0	39.0
13	36.5153593802696	38.0	35.0	39.0	33.0	39.0
14	37.22565381352901	39.0	36.0	40.0	33.0	40.0
15	37.2297233492422	39.0	36.0	40.0	33.0	40.0
16	37.22618115668019	39.0	36.0	40.0	33.0	40.0
17	37.21817903095586	39.0	36.0	40.0	33.0	40.0
18	37.044914102341366	39.0	36.0	40.0	32.0	40.0
19	36.96590256415497	39.0	36.0	40.0	32.0	40.0
20	36.67782399411338	38.0	35.0	40.0	31.0	40.0
21	36.801743502744024	38.0	35.0	40.0	31.0	40.0
22	36.794170609817165	38.0	35.0	40.0	31.0	40.0
23	36.75144559474292	38.0	35.0	40.0	31.0	40.0
24	36.805786466903086	38.0	35.0	40.0	31.0	40.0
25	36.744913080358515	38.0	35.0	40.0	31.0	40.0
26	36.66692556898895	38.0	35.0	40.0	31.0	40.0
27	36.5979171989494	38.0	35.0	40.0	31.0	40.0
28	36.516886222649184	38.0	35.0	40.0	31.0	40.0
29	36.47792414843279	38.0	35.0	40.0	31.0	40.0
30	36.31910392543613	38.0	35.0	40.0	31.0	40.0
31	36.26219174442253	38.0	35.0	40.0	31.0	40.0
32	36.213273513270444	38.0	35.0	40.0	31.0	40.0
33	35.99361465114615	38.0	35.0	40.0	30.0	40.0
34	35.68780059070609	38.0	34.0	40.0	29.0	40.0
35	35.607333748939695	38.0	34.0	40.0	29.0	40.0
36	35.64810882073399	38.0	34.0	40.0	30.0	40.0
37	35.64137599771076	38.0	34.0	40.0	30.0	40.0
38	35.55784116342528	37.0	34.0	40.0	30.0	40.0
39	35.46868951139	37.0	34.0	40.0	29.0	40.0
40	35.945027542437835	38.0	34.0	40.0	30.0	40.0
41	36.10110476346207	38.0	35.0	40.0	31.0	40.0
42	36.19167288372901	38.0	35.0	40.0	31.0	40.0
43	36.12685055544768	38.0	35.0	40.0	31.0	40.0
44	36.06272521947082	38.0	35.0	40.0	31.0	40.0
45	35.91072571002258	37.0	34.0	40.0	31.0	40.0
46	35.682224652270335	37.0	34.0	40.0	30.0	40.0
47	35.59079193451134	37.0	34.0	40.0	30.0	40.0
48	35.53510613291909	37.0	34.0	40.0	30.0	40.0
49	35.40839661110486	36.0	34.0	40.0	30.0	40.0
50	35.27441874725342	36.0	34.0	39.0	30.0	40.0
51	35.06952140543082	36.0	34.0	39.0	29.0	40.0
52	34.66037057098182	36.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	3.0
15	12.0
16	25.0
17	44.0
18	91.0
19	151.0
20	206.0
21	369.0
22	565.0
23	946.0
24	1262.0
25	1905.0
26	2742.0
27	3872.0
28	5443.0
29	7625.0
30	10461.0
31	14080.0
32	19398.0
33	29312.0
34	44286.0
35	48393.0
36	74262.0
37	106694.0
38	113512.0
39	3582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.07142638146532	0.7127308403764985	0.13878527118314954	0.07705750697503295
8	99.35410683808725	0.3382763237232879	0.11446207932630889	0.1931547588631463
9	95.9603061860622	2.5065151406759396	0.4854418542856851	1.0477368189761775
10	43.59165653200339	46.10369038007542	4.021706915757953	6.282946172163231
11	35.183803615775325	20.88728551134912	29.8472135637564	14.081697309119154
12	28.18444746497154	19.98344387781173	25.457797218162682	26.374311439054054
13	25.12565279154616	19.457531502621386	31.321934817933755	24.0948808878987
14	26.787805700620343	19.418082964567855	28.52885568580159	25.26525564901021
15	28.170752894766427	22.18581692199205	28.258643419963413	21.384786763278115
16	31.573342599311182	24.862594405665874	23.28444848695439	20.279614508068555
17	30.30894541589592	22.17048717922513	24.625698780774457	22.894868624104486
18	27.772997169107505	24.99872252143609	25.178387106664353	22.049893202792056
19	29.16473341577328	23.03120113644493	26.020092182853173	21.783973264928612
20	27.495426626741203	26.45361730830157	24.18174943024456	21.869206634712672
21	28.012754345982078	25.767253625484166	26.587497061799304	19.63249496673446
22	28.215924536786275	22.28515365512167	28.12966918415109	21.36925262394097
23	25.97144579913949	24.200962707845765	28.006622448875305	21.820969044139442
24	26.846876309415528	22.912037936003436	26.30195505319421	23.939130701386834
25	24.487526699301988	27.05679158703717	25.378286952344943	23.077394761315905
26	23.62497317295016	27.16287340698423	25.09867244427639	24.113480975789226
27	23.93177242485871	26.431338082146983	26.735275782072378	22.901613710921932
28	23.608621447332116	25.72412594916657	25.04900407771158	25.61824852578974
29	25.73761612280146	25.98125683451032	24.502243252358223	23.77888379033
30	27.038804688857322	23.78971680855195	27.587609479912928	21.5838690226778
31	26.534967143251336	24.998109331725413	25.206593833355477	23.260329691667774
32	30.303222311929606	23.415671085039193	24.72728387617656	21.553822726854644
33	27.66507578002841	25.120747273860744	24.93535958466617	22.278817361444673
34	24.757534568569938	26.049729685535876	28.76759087982504	20.425144866069147
35	24.107962268393138	28.543981031998282	27.33661049167595	20.011446207932632
36	26.731187850667865	26.423775409048638	23.84551707222353	22.99951966805997
37	24.206890208382305	28.29972713057875	25.10214718597022	22.391235475068726
38	27.538554303058792	27.249128759619413	24.246338746435836	20.965978190885956
39	26.969923044691306	26.368179541947285	23.874132592055105	22.787764821306297
40	26.200778750932557	25.064333820478492	24.180931843963656	24.55395558462529
41	21.984281903749654	28.450571799405207	24.997904935155187	24.567241361689952
42	23.430796431235883	24.768163190221667	25.83163854510521	25.96940183343723
43	22.653271878097886	24.208729777514336	25.800979059571382	27.3370192848164
44	23.89641181820969	24.894071477480608	25.9851403693446	25.224376334965097
45	24.91144518594978	25.020388557879997	26.135780641600835	23.932385614569387
46	25.631738699424623	27.13119193859927	25.560404296415907	21.6766650655602
47	22.57641876769308	26.46547230937465	27.02429253237131	23.933816390560967
48	24.81129086653926	24.601988778628296	25.013847867632784	25.572872487199668
49	26.216721683410153	25.252991854796676	25.238479698310663	23.29180676348251
50	24.30316099295854	24.59524369181085	26.021522958844752	25.08007235638586
51	22.503857985263007	25.21109055790044	26.968901061840185	25.316150394996374
52	23.379492892109273	24.787172071252645	27.047798137947243	24.78553689869084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	2.0
10	0.0
11	1.0
12	2.0
13	248.5
14	921.5
15	1348.0
16	1458.5
17	1569.0
18	1482.0
19	1395.0
20	1305.5
21	1216.0
22	1216.0
23	1216.0
24	1217.0
25	1218.0
26	1863.0
27	2508.0
28	2961.0
29	3414.0
30	3875.0
31	4336.0
32	5067.0
33	5798.0
34	6373.5
35	6949.0
36	8631.0
37	10313.0
38	11278.0
39	13464.0
40	14685.0
41	16174.5
42	17664.0
43	17185.0
44	16706.0
45	18483.5
46	20261.0
47	21881.0
48	23501.0
49	24117.0
50	24733.0
51	27294.5
52	29856.0
53	29190.5
54	28525.0
55	32324.5
56	36124.0
57	34669.5
58	33215.0
59	33173.0
60	33131.0
61	35838.0
62	38545.0
63	35505.5
64	31552.0
65	30638.0
66	25058.0
67	19478.0
68	16394.5
69	13311.0
70	10881.0
71	8451.0
72	6274.5
73	4098.0
74	4356.0
75	4614.0
76	4106.5
77	3599.0
78	2304.0
79	1009.0
80	583.0
81	157.0
82	288.0
83	419.0
84	217.5
85	16.0
86	11.0
87	6.0
88	5.5
89	4.5
90	4.0
91	2.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	489245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.769226052386841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.08840873598582	6.058518738055575
2	10.274339789758292	1.801960163108463
3	3.9717502272568352	1.04487526699302
4	1.8623406288604527	0.653251438440863
5	0.8554180360347762	0.3750677063638872
6	0.5057921357480829	0.26612433443366823
7	0.3659417756334056	0.22463183067788123
8	0.24939980887117452	0.17496346411307218
9	0.258723166212153	0.20419217365532605
>10	6.789734983567583	16.573291500168626
>50	3.1396405845745052	19.40581917035432
>100	2.5615924294338392	46.59322016576562
>500	0.055940144045870915	3.0105570828521495
>1k	0.018646714681956972	2.5355394536479676
>5k	0.0023308393352446215	1.0779875113695592
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5274	1.0779875113695592	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2591	0.5295915134544043	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2456	0.5019979764739547	No Hit
AGGGTCGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATG	1523	0.3112959764535151	No Hit
AGGGTCGGGACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA	1500	0.3065948553383274	No Hit
AGGGTCGGGGCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA	1188	0.2428231254279553	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1128	0.23055933121442224	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1014	0.20725812220870934	No Hit
AGGGTCGGGATTACCACCTGCTGTCTGACCCCTGTGCTGATGAATAATCAAC	1005	0.2054185530766794	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	974	0.19908225939968727	No Hit
AGGGTCGGGATCTTCATATGTGTGACTTTATGTGTGTAGCTAACCAATGCCA	850	0.17373708469171886	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	839	0.1714887224192378	No Hit
AGGGTCGGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGC	799	0.16331285961021574	No Hit
AGGGTCGGGGGAGCCGGTCTATTTCTAGTTCTTAAACTTTGGGATAGGAGCC	655	0.13387975349773631	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTACTC	641	0.1310182015145786	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	610	0.12468190783758648	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	605	0.12365992498645872	No Hit
AGGGTCGGGACAATTGAGAAACAGACTACCTGTAGCAATTATTCCAATGGAG	592	0.12100276957352657	No Hit
AGGGTCGGGAGCTGAGCCCTGACTGTCCAGATCAGGTGGCTGCGTAGGTATC	586	0.11977639015217324	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	581	0.11875440730104549	No Hit
AGGGTCGGGGAACAATGCAGGGCCTCCTGGGGAAGGAGATGTTGTGGGAGCT	576	0.11773242444991774	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT	576	0.11773242444991774	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	571	0.11671044159878997	No Hit
AGGGTCGGGGTGAGCCCTCTCACGCACCCCCTGCCCAGTCCTTTTCTCACCT	571	0.11671044159878997	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	556	0.1136444930454067	No Hit
AGGGTCGGGGTACGGGGACGACAGCGGGCAAGGTTTATACGTGCCTTTTTGT	548	0.11200932048360229	No Hit
AGGGTCGGGAGCTGGCAAATTGCTCTCTTACCCTGTTCTTGCTGGTGCAGTC	530	0.10833018221954235	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	526	0.10751259593864014	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	515	0.10526423366615908	No Hit
AGGGTCGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC	513	0.10485544052570797	No Hit
AGGGTCGGGGCGCGGCCGCCGACTGGGCAGGCACCACTTGGCCTAGCCCGCT	509	0.10403785424480576	No Hit
AGGGTCGGGGTGAGGGGCCCAGATGCGTGTGCACAGGTACACAAACTCTACA	505	0.10322026796390357	No Hit
AGGGTCGGGGCTCCGAGTCCGCCATTTTGGCTGCCTCTGTCGGTCTTTTCAG	501	0.10240268168300135	No Hit
AGGGTCGGGGAGCCGACTCCACAGGAGCCAAGGGCACGCGACTCAAGGAGGG	500	0.10219828511277582	No Hit
AGGGTCGGGAGCCAGGGCTACACAGAAAAACTCTTATTTTAAAAATAACAGC	498	0.1017894919723247	No Hit
AGGGTCGGGGCCAGGCGTGGTGGCGCACGCCTTTAGTCCCAGCACTCGGGAG	496	0.1013806988318736	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	492	0.1005631125509714	No Hit
AGGGTCGGGAGTCAATGATATAGAGTTATTTATTTGCTACATAATAGACATT	491	0.10035871598074585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.131897106766549E-4	0.0	0.0	0.0	0.0
8	0.0010219828511277582	0.0	0.0	0.0	0.0
9	0.0014307759915788614	0.0	0.0	0.0	0.0
10	0.012059397643307546	0.0	0.0	0.0	0.0
11	0.01757810503939744	0.0	0.0	0.0	0.0
12	0.02432319185684064	0.0	0.0	0.0	0.0
13	0.02902431297202833	0.0	0.0	0.0	0.0
14	0.03556500321924598	0.0	0.0	0.0	0.0
15	0.04271888317714029	0.0	0.0	0.0	0.0
16	0.05191672883729011	0.0	0.0	0.0	0.0
17	0.056617849952477796	0.0	0.0	0.0	0.0
18	0.0629541436294699	0.0	0.0	0.0	0.0
19	0.07072121329804086	0.0	0.0	0.0	0.0
20	0.07746630011548407	0.0	0.0	0.0	0.0
21	0.0815542315199951	0.0	0.0	0.0	0.0
22	0.08380259379247616	0.0	0.0	0.0	0.0
23	0.08686854234585943	0.0	0.0	0.0	0.0
24	0.09218285317172378	0.0	0.0	0.0	0.0
25	0.09586199143578371	0.0	0.0	0.0	0.0
26	0.10199388854255026	0.0	0.0	0.0	0.0
27	0.10935216507067011	0.0	0.0	0.0	0.0
28	0.11732363130946663	0.0	0.0	0.0	0.0
29	0.12427311469713538	0.0	0.0	0.0	0.0
30	0.12815664953142086	0.0	0.0	0.0	0.0
31	0.13571932262976627	0.0	0.0	0.0	0.0
32	0.14082923688540505	0.0	0.0	0.0	0.0
33	0.14716553056239717	0.0	0.0	0.0	0.0
34	0.1520710482478104	0.0	0.0	0.0	0.0
35	0.15983811791638136	0.0	0.0	0.0	0.0
36	0.16964915328720784	0.0	0.0	0.0	0.0
37	0.17884699894735767	0.0	0.0	0.0	0.0
38	0.18640967204570308	0.0	0.0	0.0	0.0
39	0.19458553485472513	0.0	0.0	0.0	0.0
40	0.2007174319614917	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	35	1.01796104E-7	46.000004	6
CGGAAAC	35	1.01796104E-7	46.000004	22
TTGGCGA	35	1.01796104E-7	46.000004	13
GTACCGT	55	1.8189894E-12	46.000004	36
GCGACAA	35	1.01796104E-7	46.000004	21
CGACGGC	35	1.01796104E-7	46.000004	44
GCGAAAT	35	1.01796104E-7	46.000004	16
CGTGATC	55	1.8189894E-12	46.000004	18
TGGCGAA	35	1.01796104E-7	46.000004	14
CCGTGAT	55	1.8189894E-12	46.000004	17
TTCGTAA	35	1.01796104E-7	46.000004	19
ATAGACG	55	1.8189894E-12	46.000004	27
CGACAAT	35	1.01796104E-7	46.000004	22
AATGCGC	55	1.8189894E-12	46.000004	39
CCCGTCG	35	1.01796104E-7	46.000004	35
TCAATAT	70	0.0	46.000004	28
TCGGAAT	35	1.01796104E-7	46.000004	5
GTTCGCA	55	1.8189894E-12	46.000004	38
CGTAGCT	35	1.01796104E-7	46.000004	38
TGCGAAT	35	1.01796104E-7	46.000004	43
>>END_MODULE