FastQCFastQC Report
Fri 17 Jun 2016
SRR1527351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243924
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21540.8830619373247405No Hit
GGGTTTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC10380.4255423820534265No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9140.37470687591216933No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8280.33944999262065234No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA8250.33822010134304126No Hit
GGGTTTGGGACTCACTGCTATGAAAACATGCAGGACTCCGTTTCCTTCCT8100.33207064495498595No Hit
GGGTTTGGGATCAACACAGAGTACAGTGCTGGGTTTGGGGCTGCTGCTGC6920.28369492136895097No Hit
GGGTTTGGGATTTTATTGAAATGTATGACTAACTGAAGAAGAGATTCTTG6670.27344582738885886No Hit
GGGTTTGGGCAGGCCGAGTGAAGAGGAAGAATGCCAAGAAAGGCCAGGGC5970.24474836424460078No Hit
GGGTTTGGGGCTCAGGGAACAGGTGGCACAGCTTAAGCAGAAAGTCATGA5540.22711992259884226No Hit
GGGTTTGGGGTGCTCACTACCTCCTTCCTGACCCCCCACCTGAGCGAGGC5490.22507010380282386No Hit
GGGTTTGGGCAGTGCTGGGTTTGGGATTGGTAATGGTCTGAGCTGTGTTT5330.21851068365556486No Hit
GGGTTTGGGCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAA5280.2164608648595464No Hit
GGGTTTGGGGATTCGAAGGCATGAGGTGGTGGATGCCTTGGTGGGCGCCA5150.21113133598989847No Hit
GGGTTTGGGATCATTCCTAATTATTTCAATTATCCCAAGTGGTATTTGGA4630.1898132205113068No Hit
GGGTTTGGGCGAAGAAGAGAGATTCGAAGACTGACAGCACAGAATCCAGT4590.18817336547449207No Hit
GGGTTTGGGAATCCAGCCCCAGGGGCAGCCAGCAGGATCTCTGGCTCTGG4480.1836637641232515No Hit
GGGTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA4400.18038405404962202No Hit
GGGTTTGGGCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCG4390.17997409029041833No Hit
GGGTTTGGGATGCTGATTCGAGCCCTTCCTGATGTGACTGACTGTGAAGA4370.17915416277201096No Hit
GGGTTTGGGGATGCAGACGGCGAGACAAGTTCGCTCCCAGGACCCTGGAC4350.1783342352536036No Hit
GGGTTTGGGATTCCTGGACCACGTCCAGGTCAACCTGTCGTACCTGTGGC4340.17792427149439988No Hit
GGGTTTGGGGATTGATTTACATAGTCCTTCTGAGATTGTTAAGCAGATTA4260.17464456142077042No Hit
GGGTTTGGGGGCCGCCGCGGTGCACTTCAACTACCTCATCTCAGTCTGAG4240.17382463390236302No Hit
GGGTTTGGGAAGGAGAACGTGGTACAAAGTGTCACCTCAGTGGCTGAGAA4230.17341467014315934No Hit
GGGTTTGGGATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACT4190.1717748151063446No Hit
GGGTTTGGGAGCTTGCTTATTTAATCTTCCAGGTTGCTGTTTAGTTTTCA4150.17013496006952986No Hit
GGGTTTGGGCTGTCTTGCCTGTCCCATAGATAAGGCCTGTGTGCTGCTCT4150.17013496006952986No Hit
GGGTTTGGGGGAGGGCCAGGCCCACCATCCCCAGCTAGGAGGTGACATTT4100.16808514127351143No Hit
GGGTTTGGGGTGAACTGTGCCTGGACGATGTGCTGTATGCCCGTACCACA4080.16726521375510403No Hit
GGGTTTGGGAATCCCAGCATTTGGGAGACAGAGGCAGATGAATCTCTGAG4050.16603532247749297No Hit
GGGTTTGGGACTGCATGGGCGAGGGGAAGGGTGCAGGAGCCTTGACCGCT4020.16480543119988192No Hit
GGGTTTGGGATCACTTCCATCCTATCCAGTCATATCTAATGTAGAAAAAT4010.16439546744067823No Hit
GGGTTTGGGTAGAAATGGGGCATACCACCATTTCTACCCACCTCACACTA3940.16152572112625244No Hit
GGGTTTGGGAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCG3860.15824601105262293No Hit
GGGTTTGGGAGTGTGTGTCTTGTAGTTGCTGCCACAGGCTGCAGAAATTC3850.15783604729341927No Hit
GGGTTTGGGGTGTGTCTTTGTTGTGTGAATTGTGCTGTTGTTTAGAGGGA3800.15578622849740084No Hit
GGGTTTGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA3760.15414637346058607No Hit
GGGTTTGGAAAAGGAGAGATGAAAGGCTCTGCTATCACAGGTCCAGTGGC3710.15209655466456765No Hit
GGGTTTGGGGAGAGCCATGCGCCGCCGGCTCACCAAGCACGAGGAGAAGC3660.15004673586854922No Hit
GGGTTTGGGTGCTGGGTTTGGGGAATTAGGCAGTCTTAAAAAAAAAAAAA3610.1479969170725308No Hit
GGGTTTGGGTGCAGTGCTGGGTTTGGGAATTCCATTAGTCCTGAGTCCCC3610.1479969170725308No Hit
GGGTTTGGGGGGAAAGATAAGAAAGACTTGACTTTTTGTTTTCTCGCTGT3610.1479969170725308No Hit
GGGTTTGGGACCATGGCAATATGTATTTCCCTTGAAACATTGCAAACAAA3590.14717698955412342No Hit
GGGTTTGGGATTGATGGGACCCAGTGGTTTGGGTGGCCCCTCCCCAATTC3590.14717698955412342No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC3570.14635706203571602No Hit
GGGTTTGGGTTTCCATCCAGGTTCCCACTGGAATGGAGAAGTCATTCACA3570.14635706203571602No Hit
GGGTTTGGGAGTCCAGTGTTCAGGGAACAATGGTCCTCAGTACACTTGGG3560.14594709827651237No Hit
GGGTTTGGGGTTTCCCAAGACAGGGTTTCTACGTGTAGCCCTGGCTGTCC3550.14553713451730868No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGC3520.14430724323969762No Hit
GGGTTTGGGCACAGGTTCGAACCTCAGATATGGGTGGTTATGCCACATGT3520.14430724323969762No Hit
GGGTTTGGGAGATCGGACCCACTTTTGGCTCTCCCTCCCCCCAGCCTGGA3500.14348731572129023No Hit
GGGTTTGGGCTTACAACAGAGTCAACAAGTCTTGCAACAGATAGGCAGGA3500.14348731572129023No Hit
GGGTTTGGGGTTCCCTCCCTACCCCCTCAATCTGCCTTTGCTCTCCCGTC3470.14225742444367917No Hit
GGGTTTGGGGCTAAGGAATTATTTCGCACACGGACCATTCTTAGCAGTTT3460.14184746068447548No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAGGGAAAAGGGATAAATTTG3420.14020760564766074No Hit
GGGTTTGGGAGTCATGATGGTCCGGATGCGGGTACCGTGCTTCACGGACT3410.13979764188845706No Hit
GGGTTTGGGGAATGCCTGCCGGGCATGGTGGTGCATGCCTTTAATCCAAG3400.13938767812925337No Hit
GGGTTTGGGATGTGTAACTTTTTTTTGATTGTGATTAAGAATCATGGATT3390.1389777143700497No Hit
GGGTTTGGGGAAGCCCATCCACAGGACGACCATGTTGAAGAGGAATGTTT3380.138567750610846No Hit
GGGTTTGGGATCTGTGGTAGATGAAGAGACAGCAACATCCTTTGCTAACC3380.138567750610846No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT3370.13815778685164232No Hit
GGGTTTGGGATTTGATGAAGAATTCCTTACCTGGGCCTCTGATACCCTTT3350.13733785933323495No Hit
GGGTTTGGGATGATCCAACAGACTGAGATCACGTGTCCAAAGGTGAATCA3350.13733785933323495No Hit
GGGTTTGGGGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCC3350.13733785933323495No Hit
GGGTTTGGGAACCATCAGGCAGGGGCTTCCACTATGGGGCAGTTCTCCAG3330.13651793181482758No Hit
GGGTTTGGGCACTATCGAGGCAGAGCAGAACTGGACTCACCTTCATTTTA3290.13487807677801283No Hit
GGGTTTGGGGAGGGGTTGGTGCCTCTGAAGCCACCAACCCCTATGCTGGG3270.13405814925960544No Hit
GGGTTTGGGAACTGCTAAATTCTTAGAACACAGGAAAGATCTCGAAGCTT3270.13405814925960544No Hit
GGGTTTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC3260.13364818550040178No Hit
GGGTTTGGGGCCGGAAGTCTCTGAGCCTGGCGGCGTCCGAGCTGAAGCTT3210.13159836670438332No Hit
GGGTTTGGGTCATAAATAAATAAAAGTTTAAAAAAAAAAAAGAAATTGGG3210.13159836670438332No Hit
GGGTTTGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT3120.12790869287155016No Hit
GGGTTTGGGACCTCTACAGTCTCCATGACCCCCAGCCTCTTAGCCCCTGC3110.12749872911234647No Hit
GGGTTTGGGAACAAAGGGCAAGCATGCACGCATGCACGCAGGAGAAACCG3100.12708876535314279No Hit
GGGTTTGGGGTCACGGCCATCATCTTCTGTGTCGCCTTGAGCGCTTATGA3090.1266788015939391No Hit
GGGTTTGGGGAAAAGACGCTCAACAGTGGGCCTTTGTGTGGTTTGAGATG3070.12585887407553173No Hit
GGGTTTGGGGAGCAGCTTTCCGGAAAACTGCAGTGGGCTCTAAGGAAACA3050.12503894655712433No Hit
GGGTTTGGGGAAAGGCCTTGTCACCAAGGGCTAGGGAGGGCATGGGATGC3050.12503894655712433No Hit
GGGTTTGGGGTTTGCTTCTGGTATATTTAGGTGATGCATCCGCCTGGGTT3040.12462898279792067No Hit
GGGTTTGGGGCAGGCATCCCTCTCACAGCCGGGTTCTGGGAGGGAGAGCA3030.12421901903871697No Hit
GGGTTTGGGTGTGCTGGGTTTGGGAGGACAAATGGGACTGATGTCATCTT3030.12421901903871697No Hit
GGGTTTGGGGTCCCTTCTTCCTGTCTCCCTGTCATCTGTCTGGGAGTCAG3020.12380905527951329No Hit
GGGTTTGGGACAGCCAGCCACTGAGGATTGGTCAGCAGCTCCCACAGCGC3020.12380905527951329No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGGATCTAGCCCAGCCACATTG3000.12298912776110592No Hit
GGGTTTGGGATCTGGATGGGTGATATTTTTGTATTATTTATAGCTGAGCA3000.12298912776110592No Hit
GGGTTTGGGCACTCCTCCCCGACTTCCTCCCACTAGGGGCTCCCCATCTT2970.12175923648349488No Hit
GGGTTTGGGAGCTGCCAGCTTGCAGTCGCAGAGTCCTGAGTATCTCCTGC2970.12175923648349488No Hit
GGGTTTGGGGAGAAGTTTGCTGAGGAAGACAAAAAGCTCAAAGAGCGCAT2970.12175923648349488No Hit
GGGTTTGGGATTCTCTAATAGGTTTTTCTGATCCCTCCGTGTGGTGCTGT2950.12093930896508748No Hit
GGGTTTGGGATTTTTTGTAACCACTGTCTTAATGGCAAAATAATTATGGT2920.11970941768747644No Hit
GGGTTTGGGGGAGCAATCCAGGTCGGTTTCTATCTATTTACGATTTCTCC2910.11929945392827274No Hit
GGGTTTGGGACCCCTGTAGTCTACCTCTACAGTCTCCATGACCCCCAGCC2910.11929945392827274No Hit
GGGTTTGGAATCGGAGAGATCATAGGCAAACGTGGCATTGTTGGCTATGA2890.11847952640986538No Hit
GGGTTTGGGAGCACAGACACTGACACTTCAGCTTTCAGGCTTTCCTGATC2890.11847952640986538No Hit
GGGTTTGGGAGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCT2880.11806956265066168No Hit
GGGTTTGGGGTTCATGAGTTAAATGAGGAAATAGGCAAGTTGTTGGCCAA2870.117659598891458No Hit
GGGTTTGGGATGTGTCTTTAAAAGTATTTATTGTGCAAATGTACTATAGC2860.11724963513225432No Hit
GGGTTTGGGAAATGAATAGCAGAGAATTATCTTAGGGATTGAAGGTGGTT2850.11683967137305062No Hit
GGGTTTGGGATCTGTGGATTCTGTGGCCCTGTTTCTCCATCCCCTATTTA2840.11642970761384694No Hit
GGGTTTGGGATTAAACGTTTTGCAGTTGGTCACCTCACGAATCTGATGTT2840.11642970761384694No Hit
GGGTTTGGGGTGAGAGATTTCCAACTGTTCCATTGTGTTCTATATAGAGC2830.11601974385464324No Hit
GGGTTTGGGAGACGTAGAAAATGAGAATACTTGCTGCAAAATTAAGATGC2830.11601974385464324No Hit
GGGTTTGGGGCCTCAGTTTTCTTGTCCTGCTTGGGGTCGTCTGCCTCAGT2820.11560978009543957No Hit
GGGTTTGGGTCCTTGTCCGCAGTGAGCGTGTCTGTGTCCCGCTTGTACTG2790.1143798888178285No Hit
GGGTTTGGGGTACGGGGACGACAGCGGGCAAGGTTTATACGTGCCTTTTT2780.11396992505862483No Hit
GGGTTTGGGAGCGATAATCCTCCATTTCAATTTTAACCAATTCTGTCCTC2760.11314999754021744No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC2730.11192010626260639No Hit
GGGTTTGGGATAGCACTGTGCTGTGTGCCAACCAAGCAAATGTTTACTCA2720.11151014250340269No Hit
GGGTTTGGGATTCGTCATATCCGCATCATTAATGGACGGGCTGTGGAGGC2710.11110017874419902No Hit
GGGTTTGGGGATAGTTTTTTATACCTTCTTATAGAGGGTTATAGAGATAC2710.11110017874419902No Hit
GGGTTTGGGGAGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATT2710.11110017874419902No Hit
GGGTTTGGGGGACATGGGGACCTCGTGGAAGCCACAGCAACCTGGTTGGG2700.11069021498499533No Hit
GGGTTTGGGAACACCAGGACTTAGGACAAATGGGACTGATGTCATTTTGA2700.11069021498499533No Hit
GGGTTTGGGATGTTCTCCTCAGACCATCCCCTCATCTGCTTGTCTGTGCT2680.10987028746658795No Hit
GGGTTTGGGACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAG2680.10987028746658795No Hit
GGGTTTGGGGACACGGGACCGCCCAACAACACTTGGAAATGATTGTTAAC2670.10946032370738427No Hit
GGGTTTGGGATTGACAAGGACGCCATTGTGCAAGCTGTGAAAGGCCTTGT2670.10946032370738427No Hit
GGGTTTGGGAGATCCTGCTGCTTCTACTGATACCCTTTTGACTGGAATAG2670.10946032370738427No Hit
GGGTTTGGGAGTGCCTTATCAGTTTAACACTATCTACCTACCGTCCATCT2660.10905035994818058No Hit
GGGTTTGGGGGGCAGCCCCCTGTCTCAGCTCAGCATCCCCTCCAGGACAG2650.10864039618897689No Hit
GGGTTTGGGGTTGAATACTACCATTCTTTTATGATATAAATCTTATTGAC2640.1082304324297732No Hit
GGGTTTGGGGTGTGGCAGTTTTAAGTTATTAGTTTTCAAAATCAGTACTT2630.10782046867056953No Hit
GGGTTTGGGACACCACAGCTTCCTCGATGAAATGTTTCTATGCTTTGCTT2630.10782046867056953No Hit
GGGTTTGGGAACTTGGGGCTGGGGCCTCCCTTTCACAGATACCTTGGGTT2630.10782046867056953No Hit
GGGTTTGGGATCTTGGTGTGTTTCTGCCTGCCATCGTCTGTGCTTGAATC2620.10741050491136583No Hit
GGGTTTGGGAACTGGCTTTTTCTAAGTAACTAGCTGCAAGAAGGAATGTA2620.10741050491136583No Hit
GGGTTTGGGGTGCTTCCTGTCCACTCCTGAGCGCTGCAGATGATCCGTCA2620.10741050491136583No Hit
GGGTTTGGGATTCCCCTGAGTCTTTATTACCATCACCCCTGGGCTCCCAG2610.10700054115216215No Hit
GGGTTTGGGAGCTAGGCATGGTGGTACACTTTTAATCTCAACACTTGGGA2610.10700054115216215No Hit
GGGTTTGGGGGCCCGCTCCAGCAGCTGGAGCTGCGCCTGCTGGTGGTGCT2580.10577064987455109No Hit
GGGTTTGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAG2570.1053606861153474No Hit
GGGTTTGGGATATATTATATGTGTCTGTAGTGTGCATTGCAATATTATGT2570.1053606861153474No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2560.10495072235614372No Hit
GGGTTTGGGATAACTTGCTTTACACTCATCTTGTTGTGTCAGAAATAAAG2560.10495072235614372No Hit
GGGTTTGGGAGTCATAAATATATTTGGAGACAATTATTCTGCCAAGGACA2560.10495072235614372No Hit
GGGTTTGGGGAACTGGAGGACAAAACCCAATTTGAGGTCAGACTGCAGCG2550.10454075859694004No Hit
GGGTTTGGGACTTTCAGTAAATAAAATAAAAGCAGCAACAGAAATAAAGA2540.10413079483773634No Hit
GGGTTTGGGGTCCGGGTTCTTGTGCTGTGCAGACGAAGGCAGATGGCTAT2540.10413079483773634No Hit
GGGTTTGGGGTACTTTGAAGAATGGAATTGTATTAGTCAGCCTGTTAATG2540.10413079483773634No Hit
GGGTTTGGGATATCAAGCGAGAGCTCAGGTTTCCTTCCATGCAGGCCACA2520.10331086731932898No Hit
GGGTTTGGGCTCTGACATAGCGCTTTGTCCTCAGCAGAAGCCAGATGTGT2520.10331086731932898No Hit
GGGTTTGGGATTCTGGGACAGTGCTGGGAGCATGGTAGGAGTGTGCAGCA2510.10290090356012528No Hit
GGGTTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT2490.10208097604171792No Hit
GGGTTTGGGATCACAGAAAAGGATAGTGTTAATTATGAGACTAAAGAACA2490.10208097604171792No Hit
GGGTTTGGGAGCTGAAGGTAGCATGTGAGAACTGCGAGCAGATAGAACTC2490.10208097604171792No Hit
GGGTTTGGGTACGAGAACCATGGTCTCACAGGAAATCGCTGATCTTTTTA2480.10167101228251423No Hit
GGGTTTGGGAAGAAAATGCCCAACAGGCTTTCCTATAGCCAGATCTTAGG2480.10167101228251423No Hit
GGGTTTGGGATTTTTTAAATACATTAAAGATTGTGCTCATCTCCGTTGCT2470.10126104852331054No Hit
GGGTTTGGGAACGCTCTGACAGCAGCTTCTGAGGCTGCATGCCTTATTGT2470.10126104852331054No Hit
GGGTTTGGGGAGATCTCCTTACTTCAGTGCCTCCTCAGCTTTCTCGCCTA2460.10085108476410684No Hit
GGGTTTGGGGGAATTCTGTTCTGTGGCCGGTTCTCCTCTCCCTGAGCAAA2450.10044112100490317No Hit
GGGTTTGGGACCTCAAATTAGCTGTGGATTTTGTCTCCTGCACTGCCAGT2440.10003115724569948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.844275E-444.014
GCATAAA254.4325003E-544.024
TCACGGC351.439821E-744.011
ACGCGAA351.439821E-744.015
TCCGGAA207.844275E-444.019
TCACGAA254.4325003E-544.035
TCGCCAG207.844275E-444.019
GTCATGC600.044.038
CGGGTTG454.765752E-1044.026
CTTCGCG254.4325003E-544.028
GTCATAC254.4325003E-544.024
CGTGGTA950.044.018
TATCAGC207.844275E-444.033
TAGAGTG302.5194913E-644.023
GTGCAAA351.439821E-744.033
ACTATTG207.844275E-444.015
ATGGGCG600.044.015
AGCGTGA302.5194913E-644.033
CTATGGG254.4325003E-544.032
CGGCGTC207.844275E-444.031
Produced by FastQC (version 0.11.5)