SRR1527348_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90357
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGA	1400	1.549409564283896	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	587	0.6496452958818907	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	480	0.5312261363259072	No Hit
GAGATCGGGGCTGAGATCGGGCAGACACAGAACAAGAGAACACAACGAGC	473	0.5234790885044878	No Hit
GAGATCGGGCCTTGTGTCCACAGCCTTAAGACGTGTAGCCCAGGCTGCAG	440	0.4869572916320816	No Hit
GAGATCGGGGCTAGAACTCTTTTAGTCTGAGTGAGGTGAACAGGTGACAA	404	0.4471153314076386	No Hit
GAGATCGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGT	351	0.3884591121883197	No Hit
GAGATCGGGAGCCGGGCGGGGGTGGCACACGCCTTTAATGTTAAGACATG	333	0.36853813207609815	No Hit
GAGATCGGGGTTCTTTCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGA	324	0.3585776420199874	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCG	321	0.3552574786679505	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCTGCTCGGT	318	0.35193731531591355	No Hit
GAGATCGGGATTTTTACTGACTTAGCTGCCTACCTACTCAAGCCAAGCAG	316	0.34972387308122227	No Hit
GAGATCGCGGGCTCGAGAGCCGGATACTGTCGTCCTGCGGCCGTGCCGCT	308	0.3408701041424571	No Hit
GAGATCGGGGTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGAC	303	0.33533649855572895	No Hit
GAGATCGGGAGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	298	0.3298028929690007	No Hit
GAGATCGGGGAAATGGGTGCCCGCGACGAGATCTTCGACCGCCCGCAGCA	284	0.3143087973261618	No Hit
GAGATCGGGGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGGGTGAG	272	0.30102814391801413	No Hit
GAGATCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	268	0.2966012594486315	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGGCCTGGCACGAAAGGGGCGGCCCCGGCGGAGAGCAGACCC	265	0.29328109609659464	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	261	0.28885421162721203	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGCCATCTTGTGGGAAGAGCTGAAGCAGGCGCTTTTGGCTC	258	0.28553404827517515	No Hit
GAGATCGGGGCCCTCGGGTCCCGCAGTCCTACCAGGGCCGCCAGGGGTCG	254	0.2811071638057926	No Hit
GAGATCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	254	0.2811071638057926	No Hit
GAGATCGGGAGCAAACACAGCCATAGAAGGCAGCGGAGGAGCCGAGCCGG	249	0.2755735582190644	No Hit
GAGATCGGGTGTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAA	248	0.27446683710171876	No Hit
GAGATCGGGGCCTGCAATCCCAGCTACTCTAGAGGCTGAGGCAGGTGAAT	246	0.2722533948670275	No Hit
GAGATCGGGATTGTTCTGAGATATTCACTAGAGAAGTTGTTTGGACATGG	238	0.2633996259282624	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.2633996259282624	No Hit
GAGATCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTG	236	0.26118618369357105	No Hit
GAGATCGGGGTCACTGAGGCAGTGCATGCATGTCAGGCTGCCTTCATCTT	225	0.24901225140276903	No Hit
GAGATCGGGATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAA	223	0.24679880916807775	No Hit
GAGATCGGGGGTGTATGTCTGTAATCCCAGCTTGAACCCAGGAGGCAGAG	213	0.23573159799462132	No Hit
GAGATCGGGAAAACTAGGCTGGCCTTGAACTCAGAGATCGCCTGCCTTGT	212	0.23462487687727568	No Hit
GAGATCGGGAGACACCTAGGAGCATAAATCTCTAACTTGTCACTTCAAAG	212	0.23462487687727568	No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	210	0.2324114346425844	No Hit
GAGATCGGGGGAATTCGTGGAGAAAGAAATGGCCGGTGGGCTGCAATAAA	199	0.22023750235178238	No Hit
GAGATCGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCG	190	0.21027701229567164	No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC	186	0.20585012782628906	No Hit
GAGATCGGGATTTAGTAAGGGCTGTCATTTTCCGTGTATTTTGATTTTAT	184	0.20363668559159778	No Hit
GAGATCGGGGTGTGTGTGTGTGTTTGTCTGTCTGTCTGTCTGTGTATCTG	184	0.20363668559159778	No Hit
GAGATCGGGGTCCCGCTCCTCCTGTCCCGAGGTGGCTGCCCGCTCCTCCC	183	0.20252996447425214	No Hit
GAGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCCTCAAGTAGCCGA	183	0.20252996447425214	No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT	174	0.19256947441814137	No Hit
GAGATCGGGTCTCCCAGAGCTGACGAGTGAGACATTGTCTCATGTAGTTC	174	0.19256947441814137	No Hit
GAGATCGGGGCTCGTCTCCGCCCCCTCCCGCCAAAGTGCGCGCGAAATCT	170	0.18814258994875882	No Hit
GAGATCGGAAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAACCC	170	0.18814258994875882	No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG	169	0.18703586883141318	No Hit
GAGATCGGGCCACCCCAATTAGATCCCTTGGACCTAAATGGTGGCAAGAG	169	0.18703586883141318	No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA	165	0.1826089843620306	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA	163	0.18039554212733933	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGAAGCGTCCACTGGCTGCCGGGCGGCCGCAGTCTCAGCTCTG	163	0.18039554212733933	No Hit
GAGATCGGGGTTTAACCGGATCCCATTGTACCGGGAGCGCAGAGCGGCCT	160	0.1770753787753024	No Hit
GAGATCGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAG	155	0.1715417731885742	No Hit
GAGATCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	153	0.16932833095388294	No Hit
GAGATCGGGTGCCGGGTAAACGCATTGGGCGTGCTTTTCGCCGAGGCTGG	152	0.1682216098365373	No Hit
GAGATCGGGGCTTTTCGACGGCAACAACTATGGAGGCGTTTAGTGTAAGA	152	0.1682216098365373	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	152	0.1682216098365373	No Hit
GAGATCGGGAGGCTTATCAGGCGTGGACCTGGCACCCAAAGCCTGCGCTG	151	0.16711488871919164	No Hit
GAGATCGGGAAGAGGTACTGAGCATTGAGGTATGTAAATCAGGTGACATA	148	0.16379472536715473	No Hit
GAGATCGGGAGCCAAGTGGTGGTGGCACATGCCTTTAATCCCAGAACTGA	143	0.1582611197804265	No Hit
GAGATCGGGCATGGAATTTAAGACAGAAGTCAAAAAAGATTGTTTATCCA	143	0.1582611197804265	No Hit
GAGATCGGGGCTGAGATCGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGC	141	0.15604767754573526	No Hit
GAGATCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATA	139	0.15383423531104395	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAA	138	0.15272751419369832	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGCTCG	137	0.15162079307635268	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA	137	0.15162079307635268	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGA	135	0.1494073508416614	No Hit
GAGATCGGGGTGGATGGCAGGAAAAAAATACTGTGGGACAGCAAAAAGCG	134	0.14830062972431576	No Hit
GAGATCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC	130	0.1438737452549332	No Hit
GAGATCGGGGTATCCACGCAGCCTACCAGCTCTCAGAGGGCACCAAGCCA	130	0.1438737452549332	No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC	130	0.1438737452549332	No Hit
GAGATCGGGGGACTCGGCGCCCGCACCTCGGGCCGTCAGGACCCGGGCTG	126	0.13944686078555063	No Hit
GAGATCGGGAGAGTTAGCGATGCATAATCACTGGCTTTAGAATTGCTCCT	125	0.13834013966820502	No Hit
GAGATCGGGGGACGGTTTGAACGAGACGAAGACGGAACCGGAGCCGGGCG	125	0.13834013966820502	No Hit
GAGATCGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAA	124	0.13723341855085938	No Hit
GAGATCGGGCACAAAGATCTGAAAGAAAAAGCTTAAGTTCCTGAAATCAA	124	0.13723341855085938	No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAA	124	0.13723341855085938	No Hit
GAGATCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC	123	0.13612669743351374	No Hit
GAGATCGGGGAGATGAAGATGAAGGGATTCCCAGTGAGAATGAAGAAGAA	122	0.13501997631616808	No Hit
GAGATCGGGATCTGATCCTGGGTGAGAGGCGAGGGGAAGGAAGCTCTGTA	121	0.13391325519882244	No Hit
GAGATCGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	121	0.13391325519882244	No Hit
GAGATCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	117	0.1294863707294399	No Hit
GAGATCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAG	117	0.1294863707294399	No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGTAAAT	116	0.12837964961209425	No Hit
GAGATCGGGGAAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCG	115	0.1272729284947486	No Hit
GAGATCGGGCCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	112	0.1239527651427117	No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	112	0.1239527651427117	No Hit
GAGATCGGCGTTGAGGAGGAGAGAACCCGAGGAAATGACCATGCTCTGGG	112	0.1239527651427117	No Hit
GAGATCGGGGGGAGCCTCTGCTACAGTGTTGGAAGAAACAAGGAAGGAAA	111	0.12284604402536606	No Hit
GAGATCGGGGTCGCAAACACCGCCCTCCGCGGCCAGCGATAGGTCCCAAC	110	0.1217393229080204	No Hit
GAGATCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG	109	0.12063260179067477	No Hit
GAGATCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	108	0.11952588067332913	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAG	108	0.11952588067332913	No Hit
GAGATCGGGCCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	108	0.11952588067332913	No Hit
GAGATCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	107	0.11841915955598349	No Hit
GAGATCGGGGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGCGGCGCGG	107	0.11841915955598349	No Hit
GAGATCGGGAGTTTCCGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCC	106	0.11731243843863784	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA	106	0.11731243843863784	No Hit
GAGATCGGGGTTTCCCACTCTTCCTTCCACCTCTGACCGGCCGGAGCTGC	103	0.11399227508660094	No Hit
GAGATCGGGAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCC	103	0.11399227508660094	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAAGAAGAGCTCG	103	0.11399227508660094	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGT	103	0.11399227508660094	No Hit
GAGATCGGGGACGACAGGACGCCCAGGCCGGTGTGTGTCGGTGTGTATGT	102	0.11288555396925529	No Hit
GAGATCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA	101	0.11177883285190965	No Hit
GAGATCGGGGAGACCCGGACAACGCTGGGTGTGGCTGAAAACAGGGAGTC	101	0.11177883285190965	No Hit
GAGATCGGGGACCCCTGAGCTCCTAGGCTCCAAGGCTGGCTCTGAGGCTA	100	0.11067211173456401	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAGAAGAGCTCG	100	0.11067211173456401	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA	99	0.10956539061721837	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGAAAAGCAGGGTGAAGGGAGATTGCAAAGAAGCACTGTTTCA	98	0.10845866949987272	No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97	0.10735194838252708	No Hit
GAGATCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	97	0.10735194838252708	No Hit
GAGATCGGGAGCAAAATACTCAACTGTCAGGATCCATCTAGGAGAGCCTA	96	0.10624522726518146	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAGAAGAGCTCGT	96	0.10624522726518146	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTATCAACGCAGAGTCAAATGCTTTTGTTTTATTTTTTTAC	96	0.10624522726518146	No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	95	0.10513850614783582	No Hit
GAGATCGGGGTTCAACGCAGAGTGCAGTGCGAGATCGGGGCTGAGATCGG	95	0.10513850614783582	No Hit
GAGATCGGGAGCGGCCGGGGAGGTTCTAGTCTGTTCTGCCTCGCGGCAGC	94	0.10403178503049015	No Hit
GAGATCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTC	94	0.10403178503049015	No Hit
GAGATCGGGGGAAAGCAAGCTACAGTGACAGCTGTTGGTTGAGGCCCTGA	94	0.10403178503049015	No Hit
GAGATCGGGGTGGTGATGGTACTGGGGTTTGAGCCCAGGGTCTAGTAGGT	93	0.10292506391314453	No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAA	93	0.10292506391314453	No Hit
GAGATCGGGGTAGGCTCATGTGATGGTCTGATGTGATACTTCATTTCATC	93	0.10292506391314453	No Hit
GAGATCGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCT	93	0.10292506391314453	No Hit
GAGATCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA	91	0.10071162167845325	No Hit
GAGATCGGGTCTCAAAACAAAAAGAAAACGTTTCAGGGCTAGAGATAGCC	91	0.10071162167845325	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTCCA	20	7.8179815E-4	44.000004	11
CAGCTAC	20	7.8179815E-4	44.000004	21
TTGTCTC	20	7.8179815E-4	44.000004	35
CTCTGCC	40	8.167262E-9	44.000004	25
AGCCTCC	20	7.8179815E-4	44.000004	17
TTCACTA	20	7.8179815E-4	44.000004	24
GGTGATG	20	7.8179815E-4	44.000004	12
GAGGCAA	20	7.8179815E-4	44.000004	32
ACTGTCC	40	8.167262E-9	44.000004	43
ACTGCAC	40	8.167262E-9	44.000004	38
TGGTCCG	20	7.8179815E-4	44.000004	31
TGGTCCA	20	7.8179815E-4	44.000004	38
TCCTCGC	20	7.8179815E-4	44.000004	42
TTGTTCT	20	7.8179815E-4	44.000004	11
CATTGTC	20	7.8179815E-4	44.000004	33
ACTCGCA	20	7.8179815E-4	44.000004	40
GGGCATC	20	7.8179815E-4	44.000004	33
GCCTCCG	20	7.8179815E-4	44.000004	18
TCAAGCC	40	8.167262E-9	44.000004	38
ATCTCCA	20	7.8179815E-4	44.000004	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content