SRR1527347_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78737
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	1479	1.8784053240534944	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	912	1.1582864472865362	No Hit
GAGATCGGGGCTGAGATCGGGCAGACACAGAACAAGAGAACACAACGAGCGA	429	0.5448518485591273	No Hit
GAGATCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	413	0.5245310336944512	No Hit
GAGATCGGGGCTAGAACTCTTTTAGTCTGAGTGAGGTGAACAGGTGACAATT	410	0.5207208809073244	No Hit
GAGATCGGGCCTTGTGTCCACAGCCTTAAGACGTGTAGCCCAGGCTGCAGTG	404	0.5131005753330709	No Hit
GAGATCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	365	0.4635685891004229	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTT	295	0.3746650240674651	No Hit
GAGATCGGGGGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCTGCTCGGTCC	294	0.37339497313842285	No Hit
GAGATCGGGGTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTT	291	0.3695848203512961	No Hit
GAGATCGGGAGCCGGGCGGGGGTGGCACACGCCTTTAATGTTAAGACATGAC	289	0.3670447184932116	No Hit
GAGATCGCGGGCTCGAGAGCCGGATACTGTCGTCCTGCGGCCGTGCCGCTTC	283	0.35942441291895805	No Hit
GAGATCGGGGTTCTTTCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACA	277	0.3518041073447045	No Hit
GAGATCGGGAGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCC	266	0.3378335471252397	No Hit
GAGATCGGGATTTTTACTGACTTAGCTGCCTACCTACTCAAGCCAAGCAGGC	261	0.33148329248002845	No Hit
GAGATCGGGGAAATGGGTGCCCGCGACGAGATCTTCGACCGCCCGCAGCACC	259	0.32894319062194394	No Hit
GAGATCGGGGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGGGTGAGGG	234	0.2971919173958876	No Hit
GAGATCGGGGCCCTCGGGTCCCGCAGTCCTACCAGGGCCGCCAGGGGTCGTT	232	0.29465181553780306	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	227	0.2883015608925918	No Hit
GAGATCGGGGGTGTATGTCTGTAATCCCAGCTTGAACCCAGGAGGCAGAGGT	224	0.284491408105465	No Hit
GAGATCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	222	0.2819513062473805	No Hit
GAGATCGGGAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	221	0.28068125531833826	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGTGTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATG	218	0.2768711025312115	No Hit
GAGATCGGGGCCTGCAATCCCAGCTACTCTAGAGGCTGAGGCAGGTGAATAG	218	0.2768711025312115	No Hit
GAGATCGGGGGCCTGGCACGAAAGGGGCGGCCCCGGCGGAGAGCAGACCCAG	209	0.2654406441698312	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA	207	0.2629005423117467	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAC	199	0.25274013487940866	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	197	0.25020003302132415	No Hit
GAGATCGGGGGCCATCTTGTGGGAAGAGCTGAAGCAGGCGCTTTTGGCTCGG	197	0.25020003302132415	No Hit
GAGATCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAA	195	0.24765993116323964	No Hit
GAGATCGGGATTGTTCTGAGATATTCACTAGAGAAGTTGTTTGGACATGGGT	191	0.2425797274470706	No Hit
GAGATCGGGAGACACCTAGGAGCATAAATCTCTAACTTGTCACTTCAAAGAG	190	0.24130967651802837	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCTCGTA	187	0.2374995237309016	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTCACTGAGGCAGTGCATGCATGTCAGGCTGCCTTCATCTTTT	183	0.2324193200147326	No Hit
GAGATCGGGATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGC	170	0.21590865793718328	No Hit
GAGATCGGGAAAACTAGGCTGGCCTTGAACTCAGAGATCGCCTGCCTTGTGT	167	0.21209850515005652	No Hit
GAGATCGGGCCACCCCAATTAGATCCCTTGGACCTAAATGGTGGCAAGAGAG	166	0.21082845422101423	No Hit
GAGATCGGGAGCAAACACAGCCATAGAAGGCAGCGGAGGAGCCGAGCCGGGC	165	0.209558403291972	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	164	0.20828835236292978	No Hit
GAGATCGGGGCTCGTCTCCGCCCCCTCCCGCCAAAGTGCGCGCGAAATCTGT	162	0.20574825050484524	No Hit
GAGATCGGGAAGCGTCCACTGGCTGCCGGGCGGCCGCAGTCTCAGCTCTGGG	161	0.20447819957580296	No Hit
GAGATCGGGGGAATTCGTGGAGAAAGAAATGGCCGGTGGGCTGCAATAAAGG	161	0.20447819957580296	No Hit
GAGATCGGGGTCCCGCTCCTCCTGTCCCGAGGTGGCTGCCCGCTCCTCCCTC	159	0.2019380977177185	No Hit
GAGATCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	159	0.2019380977177185	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCC	158	0.20066804678867622	No Hit
GAGATCGGGTGCCGGGTAAACGCATTGGGCGTGCTTTTCGCCGAGGCTGGCC	155	0.19685789400154946	No Hit
GAGATCGGGATTTAGTAAGGGCTGTCATTTTCCGTGTATTTTGATTTTATAC	155	0.19685789400154946	No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA	148	0.18796753749825368	No Hit
GAGATCGGGTCTCCCAGAGCTGACGAGTGAGACATTGTCTCATGTAGTTCCT	147	0.18669748656921142	No Hit
GAGATCGGGGAGATGAAGATGAAGGGATTCCCAGTGAGAATGAAGAAGAAAA	146	0.18542743564016917	No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT	146	0.18542743564016917	No Hit
GAGATCGGAAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAACCCTG	145	0.18415738471112691	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAG	145	0.18415738471112691	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA	144	0.18288733378208466	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAT	142	0.18034723192400015	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTTTAACCGGATCCCATTGTACCGGGAGCGCAGAGCGGCCTTT	140	0.17780713006591564	No Hit
GAGATCGGGGTGTGTGTGTGTGTTTGTCTGTCTGTCTGTCTGTGTATCTGTG	139	0.1765370791368734	No Hit
GAGATCGGGAAGAGGTACTGAGCATTGAGGTATGTAAATCAGGTGACATAAA	133	0.16891677356261986	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAA	133	0.16891677356261986	No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAA	132	0.1676467226335776	No Hit
GAGATCGGGAGCCAAGTGGTGGTGGCACATGCCTTTAATCCCAGAACTGAGG	132	0.1676467226335776	No Hit
GAGATCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA	132	0.1676467226335776	No Hit
GAGATCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAG	131	0.16637667170453535	No Hit
GAGATCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA	131	0.16637667170453535	No Hit
GAGATCGGGGTATCCACGCAGCCTACCAGCTCTCAGAGGGCACCAAGCCACC	130	0.1651066207754931	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTA	129	0.16383656984645084	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCCTCAAGTAGCCGAGG	128	0.1625665189174086	No Hit
GAGATCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	126	0.16002641705932408	No Hit
GAGATCGGGGCTTTTCGACGGCAACAACTATGGAGGCGTTTAGTGTAAGATT	126	0.16002641705932408	No Hit
GAGATCGGGGCTGAGATCGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGCGT	125	0.15875636613028182	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAA	123	0.1562162642721973	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	120	0.15240611148507055	No Hit
GAGATCGGGGGACGGTTTGAACGAGACGAAGACGGAACCGGAGCCGGGCGCG	120	0.15240611148507055	No Hit
GAGATCGGGCATGGAATTTAAGACAGAAGTCAAAAAAGATTGTTTATCCAAA	117	0.1485959586979438	No Hit
GAGATCGGGGAAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA	116	0.14732590776890153	No Hit
GAGATCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAA	115	0.14605585683985928	No Hit
GAGATCGGGAGGCTTATCAGGCGTGGACCTGGCACCCAAAGCCTGCGCTGAG	115	0.14605585683985928	No Hit
GAGATCGGGATCTGATCCTGGGTGAGAGGCGAGGGGAAGGAAGCTCTGTAGG	113	0.14351575498177477	No Hit
GAGATCGGGGTGGATGGCAGGAAAAAAATACTGTGGGACAGCAAAAAGCGGC	113	0.14351575498177477	No Hit
GAGATCGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCC	112	0.1422457040527325	No Hit
GAGATCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGC	112	0.1422457040527325	No Hit
GAGATCGGGGGACTCGGCGCCCGCACCTCGGGCCGTCAGGACCCGGGCTGTC	112	0.1422457040527325	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAAAAAGAGCTCGTA	111	0.14097565312369026	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCACAAAGATCTGAAAGAAAAAGCTTAAGTTCCTGAAATCAAGT	111	0.14097565312369026	No Hit
GAGATCGGGGACGACAGGACGCCCAGGCCGGTGTGTGTCGGTGTGTATGTGT	111	0.14097565312369026	No Hit
GAGATCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	111	0.14097565312369026	No Hit
GAGATCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	110	0.139705602194648	No Hit
GAGATCGGGAGAGTTAGCGATGCATAATCACTGGCTTTAGAATTGCTCCTCA	110	0.139705602194648	No Hit
GAGATCGGGGCTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	106	0.13462539847847899	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAATA	106	0.13462539847847899	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	105	0.13335534754943673	No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGTAAATAT	103	0.13081524569135222	No Hit
GAGATCGGGGAGTAGCGCTTAGGCTCAGCGAGAGGCAAGACTCGCATCCACC	102	0.12954519476230997	No Hit
GAGATCGGGGTCGCAAACACCGCCCTCCGCGGCCAGCGATAGGTCCCAACCC	102	0.12954519476230997	No Hit
GAGATCGGGGGAAAGCAAGCTACAGTGACAGCTGTTGGTTGAGGCCCTGAGA	100	0.12700509290422546	No Hit
GAGATCGGCGTTGAGGAGGAGAGAACCCGAGGAAATGACCATGCTCTGGGGA	100	0.12700509290422546	No Hit
GAGATCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT	99	0.1257350419751832	No Hit
GAGATCGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACA	99	0.1257350419751832	No Hit
GAGATCGGGAGCAAAATACTCAACTGTCAGGATCCATCTAGGAGAGCCTAAG	98	0.12446499104614094	No Hit
GAGATCGGGAGCGGCCGGGGAGGTTCTAGTCTGTTCTGCCTCGCGGCAGCCG	97	0.12319494011709871	No Hit
GAGATCGGGTGTAATTATCCTGCCTCAATCTCCCAAAGTGCTGAAATTACAG	97	0.12319494011709871	No Hit
GAGATCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT	97	0.12319494011709871	No Hit
GAGATCGGGGGGAGCCTCTGCTACAGTGTTGGAAGAAACAAGGAAGGAAACG	96	0.12192488918805644	No Hit
GAGATCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC	96	0.12192488918805644	No Hit
GAGATCGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	96	0.12192488918805644	No Hit
GAGATCGGGTGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA	96	0.12192488918805644	No Hit
GAGATCGGGTGCTCAGCTGCCTTCCTCAGACACCCCAGGCATTTTCTTGCTG	96	0.12192488918805644	No Hit
GAGATCGGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCGCAG	96	0.12192488918805644	No Hit
GAGATCGGGGAGACCCGGACAACGCTGGGTGTGGCTGAAAACAGGGAGTCAT	95	0.12065483825901419	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA	95	0.12065483825901419	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTTCAACGCAGAGTGCAGTGCGAGATCGGGGCTGAGATCGGGG	94	0.11938478732997193	No Hit
GAGATCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAG	94	0.11938478732997193	No Hit
GAGATCGGGGTTTCCCACTCTTCCTTCCACCTCTGACCGGCCGGAGCTGCGG	93	0.11811473640092966	No Hit
GAGATCGGGCCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	93	0.11811473640092966	No Hit
GAGATCGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACAT	93	0.11811473640092966	No Hit
GAGATCGGGCCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	92	0.11684468547188744	No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	90	0.11430458361380291	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAAAAAAAGCTCGTA	90	0.11430458361380291	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTTTCCGAGCTGCTAGCGCTCCTGCGAACATGGTGAACGTGCC	88	0.11176448175571839	No Hit
GAGATCGGGGGTTTCCGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGC	88	0.11176448175571839	No Hit
GAGATCGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTGG	87	0.11049443082667616	No Hit
GAGATCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT	87	0.11049443082667616	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGAAGAGCTCGTATGCCGTCTTCTG	87	0.11049443082667616	Illumina Single End Adapter 2 (100% over 30bp)
GAGATCGGGGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGCGGCGCGGGA	87	0.11049443082667616	No Hit
GAGATCGGGGAGTTGTTCCAGAGCTGGCAGATCCTGGCCCGACCCTGGCGTG	85	0.10795432896859164	No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.10668427803954939	No Hit
GAGATCGGGGTGGTGATGGTACTGGGGTTTGAGCCCAGGGTCTAGTAGGTGC	84	0.10668427803954939	No Hit
GAGATCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	84	0.10668427803954939	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGAACCGGGCGGAGCGGCCAACATGGCGGAACGCAGGCGACACA	84	0.10668427803954939	No Hit
GAGATCGGGCGCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGCACAAA	82	0.10414417618146489	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	82	0.10414417618146489	No Hit
GAGATCGGGAAAAGCAGGGTGAAGGGAGATTGCAAAGAAGCACTGTTTCACA	81	0.10287412525242262	No Hit
GAGATCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG	80	0.10160407432338037	No Hit
GAGATCGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAAC	80	0.10160407432338037	No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	79	0.10033402339433811	No Hit
GAGATCGGGAAGAGGAAGACAGAAAAGGTTGTAACGTAGAGGAAGTGAGACT	79	0.10033402339433811	No Hit
GAGATCGGGGCTTTCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT	79	0.10033402339433811	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGCGC	35	1.0034273E-7	46.000004	36
TTCACTA	45	3.0195224E-10	46.000004	24
ACGCCTT	45	3.0195224E-10	46.000004	29
ACACGCC	45	3.0195224E-10	46.000004	27
CCTACTC	35	1.0034273E-7	46.000004	33
CCTACCT	35	1.0034273E-7	46.000004	29
TCAAGCC	35	1.0034273E-7	46.000004	38
CACACGC	45	3.0195224E-10	46.000004	26
GGGCAAG	35	1.0034273E-7	46.000004	45
CTTTACC	35	1.0034273E-7	46.000004	32
TCGACCG	35	1.0034273E-7	46.000004	35
GATATTC	45	3.0195224E-10	46.000004	20
ACATGGG	45	3.0195224E-10	46.000004	45
ACATGAC	45	3.0195224E-10	46.000004	46
TTACTGA	35	1.0034273E-7	46.000004	14
GGCGCTT	35	1.0034273E-7	46.000004	37
CGCCCGC	35	1.0034273E-7	46.000004	40
GACAGCG	35	1.0034273E-7	46.000004	35
CCCGGCA	35	1.0034273E-7	46.000004	38
CACGCCT	45	3.0195224E-10	46.000004	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content