FastQCFastQC Report
Fri 17 Jun 2016
SRR1527346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82724
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG10151.2269716164595523No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA7550.9126734684009477Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGCTGAGATCGGGCAGACACAGAACAAGAGAACACAACGAGCGA4470.5403510468546009No Hit
GAGATCGGGGCTAGAACTCTTTTAGTCTGAGTGAGGTGAACAGGTGACAATT4300.5198007833276921No Hit
GAGATCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA4300.5198007833276921No Hit
GAGATCGGGCCTTGTGTCCACAGCCTTAAGACGTGTAGCCCAGGCTGCAGTG4140.500459358831778No Hit
GAGATCGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTT3440.41584062666215366No Hit
GAGATCGGGAGCCGGGCGGGGGTGGCACACGCCTTTAATGTTAAGACATGAC3190.38561965088728783No Hit
GAGATCGGGGGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCTGCTCGGTCC2990.3614428702673952No Hit
GAGATCGGGAGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCC2940.35539867511242207No Hit
GAGATCGCGGGCTCGAGAGCCGGATACTGTCGTCCTGCGGCCGTGCCGCTTC2910.3517721580194381No Hit
GAGATCGGGGAAATGGGTGCCCGCGACGAGATCTTCGACCGCCCGCAGCACC2890.34935447995744884No Hit
GAGATCGGGATTTTTACTGACTTAGCTGCCTACCTACTCAAGCCAAGCAGGC2760.33363957255451865No Hit
GAGATCGGGGCCCTCGGGTCCCGCAGTCCTACCAGGGCCGCCAGGGGTCGTT2750.332430733523524No Hit
GAGATCGGGGTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTT2720.3288042164305401No Hit
GAGATCGGGGTTCTTTCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACA2700.32638653836855086No Hit
GAGATCGGGGCCTGCAATCCCAGCTACTCTAGAGGCTGAGGCAGGTGAATAG2580.31188046999661523No Hit
GAGATCGGGGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGGGTGAGGG2560.30946279193462595No Hit
GAGATCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG2400.29012136743871186No Hit
GAGATCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAA2380.2877036893767226No Hit
GAGATCGGGATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGC2370.28649485034572797No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTA2320.2804506551907548Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAAAAAGAGCTCGTA2310.2792418161597602Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGCCTGGCACGAAAGGGGCGGCCCCGGCGGAGAGCAGACCCAG2200.2659445868188192No Hit
GAGATCGGGAGACACCTAGGAGCATAAATCTCTAACTTGTCACTTCAAAGAG2200.2659445868188192No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2180.26352690875682994No Hit
GAGATCGGGGGCCATCTTGTGGGAAGAGCTGAAGCAGGCGCTTTTGGCTCGG2180.26352690875682994No Hit
GAGATCGGGATTTAGTAAGGGCTGTCATTTTCCGTGTATTTTGATTTTATAC2160.26110923069484065No Hit
GAGATCGGGGTCACTGAGGCAGTGCATGCATGTCAGGCTGCCTTCATCTTTT2120.25627387457086215No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.25264735747787825No Hit
GAGATCGGGTGTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATG2060.24902084038489433No Hit
GAGATCGGGAGCAAACACAGCCATAGAAGGCAGCGGAGGAGCCGAGCCGGGC2060.24902084038489433No Hit
GAGATCGGGAAAACTAGGCTGGCCTTGAACTCAGAGATCGCCTGCCTTGTGT2030.24539432329191047No Hit
GAGATCGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCC2000.24176780619892657No Hit
GAGATCGGGATTGTTCTGAGATATTCACTAGAGAAGTTGTTTGGACATGGGT1990.2405589671679319No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAAGAAGAGCTCGTA1980.2393501281369373Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAATTCGTGGAGAAAGAAATGGCCGGTGGGCTGCAATAAAGG1960.236932450074948No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT1940.23451477201295876No Hit
GAGATCGGGGGTGTATGTCTGTAATCCCAGCTTGAACCCAGGAGGCAGAGGT1850.22363522073400705No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1820.22000870364102315No Hit
GAGATCGGGGTGTGTGTGTGTGTTTGTCTGTCTGTCTGTCTGTGTATCTGTG1780.21517334751704462No Hit
GAGATCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1750.21154683042406072Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGAGGCTTATCAGGCGTGGACCTGGCACCCAAAGCCTGCGCTGAG1650.1994584401141144No Hit
GAGATCGGGTCTCCCAGAGCTGACGAGTGAGACATTGTCTCATGTAGTTCCT1600.19341424495914125No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA1590.1922054059281466Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCCACCCCAATTAGATCCCTTGGACCTAAATGGTGGCAAGAGAG1540.18616121077317344No Hit
GAGATCGGGGTCCCGCTCCTCCTGTCCCGAGGTGGCTGCCCGCTCCTCCCTC1510.18253469368018954No Hit
GAGATCGGAAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAACCCTG1510.18253469368018954No Hit
GAGATCGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCC1500.18132585464919493No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAC1490.1801170156182003Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA1490.1801170156182003No Hit
GAGATCGGGAAGCGTCCACTGGCTGCCGGGCGGCCGCAGTCTCAGCTCTGGG1480.17890817658720565No Hit
GAGATCGGGGAGATGAAGATGAAGGGATTCCCAGTGAGAATGAAGAAGAAAA1460.17649049852521637No Hit
GAGATCGGGTGCCGGGTAAACGCATTGGGCGTGCTTTTCGCCGAGGCTGGCC1450.17528165949422175No Hit
GAGATCGGGGCTGAGATCGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGCGT1430.17286398143223247No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA1430.17286398143223247No Hit
GAGATCGGGGTTTAACCGGATCCCATTGTACCGGGAGCGCAGAGCGGCCTTT1410.17044630337024322No Hit
GAGATCGGGCACAAAGATCTGAAAGAAAAAGCTTAAGTTCCTGAAATCAAGT1410.17044630337024322No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1400.1692374643392486No Hit
GAGATCGGGGCTCGTCTCCGCCCCCTCCCGCCAAAGTGCGCGCGAAATCTGT1390.16802862530825396No Hit
GAGATCGGGCATGGAATTTAAGACAGAAGTCAAAAAAGATTGTTTATCCAAA1390.16802862530825396No Hit
GAGATCGGGGGACGGTTTGAACGAGACGAAGACGGAACCGGAGCCGGGCGCG1370.16561094724626468No Hit
GAGATCGGGGGACTCGGCGCCCGCACCTCGGGCCGTCAGGACCCGGGCTGTC1360.16440210821527004No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA1330.16077559112228615No Hit
GAGATCGGGAGAGTTAGCGATGCATAATCACTGGCTTTAGAATTGCTCCTCA1330.16077559112228615No Hit
GAGATCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT1310.1583579130602969No Hit
GAGATCGGGAAGAGGTACTGAGCATTGAGGTATGTAAATCAGGTGACATAAA1300.15714907402930225No Hit
GAGATCGGGGCTTTTCGACGGCAACAACTATGGAGGCGTTTAGTGTAAGATT1290.15594023499830761No Hit
GAGATCGGGAGCCAAGTGGTGGTGGCACATGCCTTTAATCCCAGAACTGAGG1280.15473139596731297No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAA1270.15352255693631836No Hit
GAGATCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA1260.15231371790532372No Hit
GAGATCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA1240.14989603984333447No Hit
GAGATCGGGGTATCCACGCAGCCTACCAGCTCTCAGAGGGCACCAAGCCACC1220.1474783617813452No Hit
GAGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCCTCAAGTAGCCGAGG1220.1474783617813452No Hit
GAGATCGGGGAAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA1200.14506068371935593No Hit
GAGATCGGGGTTTCCCACTCTTCCTTCCACCTCTGACCGGCCGGAGCTGCGG1180.14264300565736665No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAA1160.1402253275953774No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT1150.13901648856438278No Hit
GAGATCGGGGTCGCAAACACCGCCCTCCGCGGCCAGCGATAGGTCCCAACCC1140.13780764953338814No Hit
GAGATCGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTGG1130.1365988105023935No Hit
GAGATCGGGGGGAGCCTCTGCTACAGTGTTGGAAGAAACAAGGAAGGAAACG1120.13538997147139886No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAA1120.13538997147139886Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGC1120.13538997147139886No Hit
GAGATCGGGGACCCCTGAGCTCCTAGGCTCCAAGGCTGGCTCTGAGGCTAAC1120.13538997147139886No Hit
GAGATCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC1090.13176345437841497No Hit
GAGATCGGGCCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA1070.12934577631642571No Hit
GAGATCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAG1070.12934577631642571No Hit
GAGATCGGGGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGCGGCGCGGGA1070.12934577631642571No Hit
GAGATCGGGAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT1070.12934577631642571No Hit
GAGATCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGA1060.12813693728543107No Hit
GAGATCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAA1060.12813693728543107No Hit
GAGATCGGGTGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA1050.12692809825443643No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGTAAATAT1050.12692809825443643No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAACGGAAGAGCTCGTA1030.12451042019244717Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC1010.1220927421304579No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC1010.1220927421304579No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAA1000.12088390309946329Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGTAGTGCTGTTACTAATTCGTCTATGTCAACAAGAAGCCATAA990.11967506406846865No Hit
GAGATCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT990.11967506406846865No Hit
GAGATCGGGGGTTTCCGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGC980.118466225037474No Hit
GAGATCGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACA970.11725738600647938No Hit
GAGATCGGGCAGGTTTTGAGTTACAAGATTAAAAAAAAAATCAATAAAATTA970.11725738600647938No Hit
GAGATCGGGAGTTTCCGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGC970.11725738600647938No Hit
GAGATCGGGGAACCGGGCGGAGCGGCCAACATGGCGGAACGCAGGCGACACA970.11725738600647938No Hit
GAGATCGGGATCTGATCCTGGGTGAGAGGCGAGGGGAAGGAAGCTCTGTAGG960.11604854697548474No Hit
GAGATCGGGCGCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGCACAAA950.11483970794449012No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG940.11363086891349547No Hit
GAGATCGGGTCTCAAAACAAAAAGAAAACGTTTCAGGGCTAGAGATAGCCTA940.11363086891349547No Hit
GAGATCGGGGGAAAGCAAGCTACAGTGACAGCTGTTGGTTGAGGCCCTGAGA940.11363086891349547No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAAGGAAGAGCTCGTA940.11363086891349547Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT940.11363086891349547No Hit
GAGATCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT930.11242202988250086No Hit
GAGATCGGGGAGTAGCGCTTAGGCTCAGCGAGAGGCAAGACTCGCATCCACC920.11121319085150622No Hit
GAGATCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAG910.11000435182051158No Hit
GAGATCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG900.10879551278951695No Hit
GAGATCGGGGTGGTGATGGTACTGGGGTTTGAGCCCAGGGTCTAGTAGGTGC880.1063778347275277No Hit
GAGATCGGGGTGGATGGCAGGAAAAAAATACTGTGGGACAGCAAAAAGCGGC870.10516899569653305No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAG860.10396015666553841Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA860.10396015666553841Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGTGCTCAGCTGCCTTCCTCAGACACCCCAGGCATTTTCTTGCTG860.10396015666553841No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.10275131763454379No Hit
GAGATCGGGGTATCAACGCAGAGTCAAATGCTTTTGTTTTATTTTTTTACCA840.10154247860354915No Hit
GAGATCGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACAT840.10154247860354915No Hit
GAGATCGGGTGTAATTATCCTGCCTCAATCTCCCAAAGTGCTGAAATTACAG840.10154247860354915No Hit
GAGATCGGGGAGACCCGGACAACGCTGGGTGTGGCTGAAAACAGGGAGTCAT840.10154247860354915No Hit
GAGATCGGGGCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACGT840.10154247860354915No Hit
GAGATCGGGAGCAAAATACTCAACTGTCAGGATCCATCTAGGAGAGCCTAAG830.10033363957255453No Hit
GAGATCGGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCGCAG830.10033363957255453No Hit
GAGATCGGGCTCCTTCCCAACATGGCGCAGTCGATTAACATCACGGAGCTGA830.10033363957255453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAT351.00426405E-746.00000418
ACAGCGG351.00426405E-746.00000439
TGATGCT351.00426405E-746.00000438
AACGCTT351.00426405E-746.00000420
GTTGATG351.00426405E-746.00000436
ACGCTTC351.00426405E-746.00000421
GGGTCGT351.00426405E-746.00000445
GGTTGAT351.00426405E-746.00000435
AATCCCC351.00426405E-746.00000446
ACATGAC351.00426405E-746.00000446
AACGAGC700.046.00000444
TAAGTTC351.00426405E-746.00000434
AACTGGT351.00426405E-746.00000431
AGGATCT351.00426405E-746.00000416
CCTGCCT351.00426405E-746.00000441
AATCAAG351.00426405E-746.00000445
TCGGTGC351.00426405E-746.0000045
GATGCTG351.00426405E-746.00000439
CACAACG700.046.00000441
CACAAAG351.00426405E-746.00000410
Produced by FastQC (version 0.11.5)