FastQCFastQC Report
Fri 17 Jun 2016
SRR1527342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430389
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC72401.68219912683642No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG45201.0502127145442843No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT37080.8615461826394262No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT23830.5536851545927057No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC20820.48374842293831855No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATG16360.380121239158064No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13660.31738729382024167No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG8510.19772810178698805No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC8300.19284879492737966No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATA8300.19284879492737966No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC8010.18611070450220615No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA7650.17774617845716317No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTG7630.1772814825657719No Hit
ATCAACGGGTGTGTGTGTGTATGTGCCTGTGTATGTGTGCCTGTGTGTAT7430.17263452365185913No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT7110.16519938938959872No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACT7030.16334060582403362No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT6900.1603200825299903No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACT6870.1596230386929034No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC6630.15404668799620808No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACT6120.14219694276573053No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG5820.1352265043948614No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATT5720.132903024937905No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5570.12941780575247044No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT5250.12198267149021003No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT5220.1212856276531231No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT5200.12082093176173184No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGAT5110.11872980025047108No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGC5030.11687101668490599No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCA4950.11501223311934088No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA4930.11454753722794961No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCG4900.11385049339086269No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGG4850.1126887536623845No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTAC4820.11199170982529759No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGA4810.11175936187960195No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC4750.11036527420542812No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCC4740.11013292625973248No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAG4530.10525361940012408No Hit
ATCAACGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT4480.10409187967164589No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG4430.1029301399431677No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC4420.10269779199747206No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4420.10269779199747206No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC4380.10176840021468951No Hit
ATCAACGGGGTCCTACCCTCCAGCCAAGGTAGAATCTTGCCAAGCCTAGC4380.10176840021468951No Hit
ATCAACGGGGGCTGCAGCCAATCAGGGAGTGACACGTCCTAGGCGAAGGA4360.10130370432329823No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC4340.10083900843190696No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC4310.10014196459482004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT207.850987E-444.015
ATTTACG207.850987E-444.021
CATGGTA207.850987E-444.030
GTCATAA254.4381846E-544.031
CGGAATT207.850987E-444.021
CAGCGTC254.4381846E-544.019
TAATAGG408.290954E-944.016
CCTTATA351.443259E-744.030
TCGTTTG302.5240115E-644.021
CGGAACA207.850987E-444.013
TCGTTAG302.5240115E-644.014
GTCGCCC302.5240115E-644.023
GGTACCG254.4381846E-544.09
ACCGGCC207.850987E-444.032
CGTATGA207.850987E-444.029
GTCGAGG302.5240115E-644.038
GATCGAG454.783942E-1044.038
CGAGTAG207.850987E-444.032
CCTAGTA302.5240115E-644.042
CGACGAC207.850987E-444.028
Produced by FastQC (version 0.11.5)