##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430389
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.377721084879028	31.0	30.0	33.0	27.0	33.0
2	30.94008908220238	31.0	31.0	33.0	28.0	34.0
3	31.776353484870665	33.0	31.0	34.0	30.0	34.0
4	35.61293155726564	37.0	35.0	37.0	33.0	37.0
5	36.03585128802084	37.0	35.0	37.0	35.0	37.0
6	36.2102237743065	37.0	36.0	37.0	35.0	37.0
7	36.36168210618766	37.0	37.0	37.0	35.0	37.0
8	36.55167302138298	37.0	37.0	37.0	35.0	37.0
9	38.47158036102224	39.0	39.0	39.0	37.0	39.0
10	36.72064574141068	39.0	37.0	39.0	32.0	39.0
11	36.840918332020564	38.0	37.0	39.0	32.0	39.0
12	36.414051009668	37.0	35.0	39.0	33.0	39.0
13	36.495333291510704	38.0	35.0	39.0	33.0	39.0
14	37.19453099405422	39.0	36.0	40.0	33.0	40.0
15	37.286605837974484	39.0	36.0	40.0	33.0	40.0
16	37.41725508783914	39.0	36.0	40.0	33.0	40.0
17	37.388669320080204	39.0	36.0	40.0	33.0	40.0
18	37.25913301687543	39.0	36.0	40.0	33.0	40.0
19	37.18055758860008	39.0	35.0	40.0	33.0	40.0
20	36.99094540055624	38.0	35.0	40.0	33.0	40.0
21	36.93783763060859	38.0	35.0	40.0	33.0	40.0
22	36.81211648067214	38.0	35.0	40.0	33.0	40.0
23	36.576120672229074	38.0	35.0	40.0	32.0	40.0
24	36.441047517478374	38.0	35.0	40.0	32.0	40.0
25	36.29605078196701	38.0	35.0	39.0	32.0	40.0
26	35.80437000016264	38.0	34.0	39.0	31.0	40.0
27	35.641935551326824	37.0	34.0	39.0	31.0	40.0
28	35.499850135575024	37.0	34.0	38.0	31.0	40.0
29	35.19853434915855	36.0	34.0	38.0	30.0	40.0
30	34.87122347457765	36.0	34.0	38.0	30.0	40.0
31	34.784708716997876	36.0	34.0	38.0	30.0	39.0
32	34.65628071349407	36.0	33.0	38.0	29.0	39.0
33	34.040433189509955	35.0	33.0	38.0	28.0	39.0
34	33.40000789983015	35.0	32.0	38.0	25.0	39.0
35	33.27942860993195	35.0	31.0	38.0	26.0	39.0
36	33.08384740316318	34.0	31.0	37.0	26.0	38.0
37	33.03284238212408	34.0	31.0	37.0	26.0	38.0
38	32.61903533779906	34.0	31.0	37.0	26.0	38.0
39	31.95945528347611	34.0	30.0	36.0	24.0	38.0
40	31.708194215000848	33.0	30.0	36.0	23.0	38.0
41	31.489710471224868	33.0	30.0	36.0	23.0	38.0
42	31.06299649851646	33.0	29.0	35.0	23.0	38.0
43	30.5266956172207	32.0	28.0	35.0	22.0	37.0
44	29.93500995610947	31.0	27.0	34.0	21.0	37.0
45	29.259277072601762	31.0	26.0	34.0	20.0	37.0
46	29.047487273141275	31.0	26.0	34.0	20.0	37.0
47	28.52762036204457	30.0	25.0	34.0	19.0	36.0
48	28.052647721015173	30.0	24.0	33.0	18.0	35.0
49	27.681994660644207	29.0	24.0	33.0	18.0	35.0
50	31.642590772533683	33.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	8.0
13	16.0
14	30.0
15	55.0
16	76.0
17	148.0
18	227.0
19	298.0
20	493.0
21	774.0
22	1077.0
23	1517.0
24	2111.0
25	2948.0
26	3874.0
27	5355.0
28	7094.0
29	9972.0
30	13334.0
31	19157.0
32	32674.0
33	47984.0
34	57315.0
35	100897.0
36	106844.0
37	15801.0
38	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.66609044376135	1.095288216009238	0.12523554272994897	0.11338579749947142
8	98.38471708152392	0.36548331857923877	0.14684390167964328	1.1029556982171942
9	96.42230633217856	2.2849096979709054	0.3643215788507606	0.9284623909997699
10	50.75966160845189	35.34895176224299	4.306801521414348	9.584585107890769
11	37.88293845799962	20.460327750012198	23.63025077313779	18.02648301885039
12	34.314306360060314	15.419306720199632	25.483457988006197	24.78292893173385
13	26.80180023188325	15.20229373891991	30.47638299305977	27.519523036137077
14	25.360778272678903	17.458392291624552	33.23853537148951	23.942294064207033
15	27.786258477795666	18.473752814314494	29.490530659473173	24.249458048416663
16	32.78383044176315	21.281910085991974	24.833348435949805	21.100911036295074
17	32.514306824756204	20.455216095206893	25.768781265320445	21.261695814716454
18	30.763565053939573	20.59601895029845	25.851264786042393	22.78915120971958
19	29.817676567012636	24.309636166351837	23.77941815427439	22.093269112361146
20	29.826505788949063	24.419769092611567	24.290815982750487	21.462909135688875
21	27.927526028778615	25.23159281487213	25.918413342348433	20.92246781400082
22	27.415198808519737	22.485472444695382	27.90963523696005	22.18969350982483
23	28.822065619706823	23.145805306362384	25.54619193334402	22.48593714058677
24	29.84648771227889	21.489629149443875	24.34100313902075	24.322879999256486
25	28.60644672610127	22.573067620222638	22.123009649410186	26.69747600426591
26	23.914179962777858	23.14441121868821	25.278062404011255	27.663346414522678
27	22.746631535657276	20.9928692415466	24.812669468782893	31.447829754013227
28	25.525048270285716	24.657693389003903	22.972241390927742	26.84501694978264
29	29.309299261830578	23.881883598326166	22.00172402175706	24.807093118086197
30	28.346914070759244	21.130883921289808	25.404227338523988	25.117974669426964
31	32.3760597970673	20.563257889955366	24.099128927551586	22.96155338542574
32	32.85655534876588	20.314645588061033	24.841015918157762	21.987783145015324
33	33.565448931083274	20.664096898387275	24.11283745634763	21.65761671418182
34	26.58246377114657	23.60678363062253	29.036755121529595	20.773997476701307
35	25.52272479082876	27.993280497410485	26.118464923592377	20.365529788168377
36	25.902381334095438	28.867838165008862	25.518077831914848	19.711702668980852
37	29.495874662224175	27.776499864076452	21.417601286278227	21.31002418742115
38	27.98468362341974	29.234250875370886	22.465258173419862	20.31580732778951
39	29.997746224926754	25.594287958103017	22.16320584401553	22.2447599729547
40	25.458596757816764	24.335659136269747	23.463657296074018	26.74208680983947
41	24.586594917621035	27.133360750390928	21.668769415575213	26.611274916412825
42	22.915083796286613	23.216439081853856	24.971363115693013	28.897114006166515
43	24.09401727274628	24.01060436024155	22.440861639121817	29.45451672789035
44	24.4234866597427	22.122777301464488	24.76736161937224	28.686374419420567
45	26.46559275446166	21.84093924333568	24.089602661778066	27.603865340424594
46	26.40982924749471	25.427229785147855	23.42253170968589	24.740409257671548
47	24.585433177892558	24.593100660100514	24.584503786109778	26.236962375897154
48	26.02831392066247	24.435568752918872	25.491822514051243	24.04429481236742
49	26.820620415484598	22.959229905968787	25.28689162594769	24.933258052598926
50	24.51665818596665	21.362302475202664	26.79111222638125	27.329927112449436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	2.5
6	2.0
7	2.0
8	2.0
9	47.0
10	92.0
11	207.5
12	323.0
13	380.0
14	437.0
15	478.0
16	519.0
17	646.0
18	773.0
19	764.0
20	755.0
21	1005.5
22	1256.0
23	1214.5
24	1173.0
25	1499.0
26	1825.0
27	1981.5
28	2138.0
29	3183.0
30	4228.0
31	5575.5
32	6923.0
33	7247.5
34	7572.0
35	9186.0
36	10800.0
37	10795.0
38	10790.0
39	11574.5
40	12359.0
41	13436.0
42	14513.0
43	15597.5
44	16682.0
45	18341.0
46	20000.0
47	22067.0
48	24134.0
49	24205.0
50	24276.0
51	25141.0
52	26006.0
53	27973.0
54	29940.0
55	33802.0
56	37664.0
57	37342.0
58	37020.0
59	39412.0
60	41804.0
61	41279.5
62	40755.0
63	31492.0
64	22229.0
65	16574.0
66	10919.0
67	8586.0
68	6253.0
69	5964.5
70	5676.0
71	5314.5
72	4953.0
73	3983.5
74	3014.0
75	2034.5
76	1055.0
77	1125.0
78	1195.0
79	743.0
80	291.0
81	162.5
82	34.0
83	19.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	430389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.269830316295259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62740493864565	8.788561045937513
2	9.271322526889865	2.2751510842516884
3	3.257082260263596	1.1989153997894928
4	1.49409180427208	0.733290116615434
5	0.7479927283744887	0.45888719274888534
6	0.5794576579306165	0.42659082829719164
7	0.41660354491743673	0.3578158363712827
8	0.36547492804120585	0.3587452281540653
9	0.35979397061051355	0.3973149871395412
>10	8.311240721102864	27.000225377507327
>50	2.243978185123466	18.861541535680512
>100	1.2725344644750796	30.574898522034715
>500	0.039766702014846236	3.239162710942891
>1k	0.01136191486138464	3.6467010076930406
>5k	0.00189365247689744	1.68219912683642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7240	1.68219912683642	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4520	1.0502127145442843	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3708	0.8615461826394262	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2383	0.5536851545927057	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2082	0.48374842293831855	No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATG	1636	0.380121239158064	No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1366	0.31738729382024167	No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	851	0.19772810178698805	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	830	0.19284879492737966	No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATA	830	0.19284879492737966	No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	801	0.18611070450220615	No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	765	0.17774617845716317	No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTG	763	0.1772814825657719	No Hit
ATCAACGGGTGTGTGTGTGTATGTGCCTGTGTATGTGTGCCTGTGTGTAT	743	0.17263452365185913	No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	711	0.16519938938959872	No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACT	703	0.16334060582403362	No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	690	0.1603200825299903	No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACT	687	0.1596230386929034	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	663	0.15404668799620808	No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACT	612	0.14219694276573053	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	582	0.1352265043948614	No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATT	572	0.132903024937905	No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	557	0.12941780575247044	No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	525	0.12198267149021003	No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	522	0.1212856276531231	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	520	0.12082093176173184	No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGAT	511	0.11872980025047108	No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGC	503	0.11687101668490599	No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCA	495	0.11501223311934088	No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	493	0.11454753722794961	No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCG	490	0.11385049339086269	No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGG	485	0.1126887536623845	No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTAC	482	0.11199170982529759	No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGA	481	0.11175936187960195	No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC	475	0.11036527420542812	No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCC	474	0.11013292625973248	No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAG	453	0.10525361940012408	No Hit
ATCAACGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	448	0.10409187967164589	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	443	0.1029301399431677	No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC	442	0.10269779199747206	No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	442	0.10269779199747206	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	438	0.10176840021468951	No Hit
ATCAACGGGGTCCTACCCTCCAGCCAAGGTAGAATCTTGCCAAGCCTAGC	438	0.10176840021468951	No Hit
ATCAACGGGGGCTGCAGCCAATCAGGGAGTGACACGTCCTAGGCGAAGGA	436	0.10130370432329823	No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	434	0.10083900843190696	No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	431	0.10014196459482004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0030205232940432956	0.0	0.0	0.0	0.0
8	0.005111654805304039	0.0	0.0	0.0	0.0
9	0.017890791818564136	0.0	0.0	0.0	0.0
10	0.022770098678172538	0.0	0.0	0.0	0.0
11	0.03438749596295444	0.0	0.0	0.0	0.0
12	0.04228732611660614	0.0	0.0	0.0	0.0
13	0.049025416541779644	0.0	0.0	0.0	0.0
14	0.054601767238474964	0.0	0.0	0.0	0.0
15	0.06250159739212666	0.0	0.0	0.0	0.0
16	0.14777329346242585	0.0	0.0	0.0	0.0
17	0.15497607977899064	0.0	0.0	0.0	0.0
18	0.16171417020416415	0.0	0.0	0.0	0.0
19	0.17031104419490276	0.0	0.0	0.0	0.0
20	0.1761197428372937	0.0	0.0	0.0	0.0
21	0.1847166168280323	0.0	0.0	0.0	0.0
22	0.19238409903598838	0.0	0.0	0.0	0.0
23	0.20191036480950952	0.0	0.0	0.0	0.0
24	0.20911315112607431	0.0	0.0	0.0	0.0
25	0.21654828538833473	0.0	0.0	0.0	0.0
26	0.2242157675962908	0.0	0.0	0.0	0.0
27	0.2425712553062462	0.0	0.0	0.0	0.0
28	0.2537239566996368	0.0	0.0	0.0	0.0
29	0.26069439507050596	0.0	0.0	0.0	0.0
30	0.2692912690612446	0.0	0.0	0.0	0.0
31	0.2795145786718527	0.0	0.0	0.0	0.0
32	0.28927319239106947	0.0	0.0	0.0	0.0
33	0.2978700663818081	0.0	0.0	0.0	0.0
34	0.3083257239381118	0.0	0.0	0.0	0.0
35	0.32249894862554573	0.0	0.0	0.0	0.0
36	0.34085443633550117	0.0	0.0	0.0	0.0
37	0.35479531307723944	0.0	0.0	0.0	0.0
38	0.37082732133023844	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	7.850987E-4	44.0	15
ATTTACG	20	7.850987E-4	44.0	21
CATGGTA	20	7.850987E-4	44.0	30
GTCATAA	25	4.4381846E-5	44.0	31
CGGAATT	20	7.850987E-4	44.0	21
CAGCGTC	25	4.4381846E-5	44.0	19
TAATAGG	40	8.290954E-9	44.0	16
CCTTATA	35	1.443259E-7	44.0	30
TCGTTTG	30	2.5240115E-6	44.0	21
CGGAACA	20	7.850987E-4	44.0	13
TCGTTAG	30	2.5240115E-6	44.0	14
GTCGCCC	30	2.5240115E-6	44.0	23
GGTACCG	25	4.4381846E-5	44.0	9
ACCGGCC	20	7.850987E-4	44.0	32
CGTATGA	20	7.850987E-4	44.0	29
GTCGAGG	30	2.5240115E-6	44.0	38
GATCGAG	45	4.783942E-10	44.0	38
CGAGTAG	20	7.850987E-4	44.0	32
CCTAGTA	30	2.5240115E-6	44.0	42
CGACGAC	20	7.850987E-4	44.0	28
>>END_MODULE