FastQCFastQC Report
Fri 17 Jun 2016
SRR1527341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383704
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC64711.6864562266747283No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT39921.0403852969997707No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32760.8537831244917958No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA21040.5483393449117028No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC21000.547296874674228No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATGTA14380.3747680503721619No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12160.3169109521923149No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC8480.22100369034464065No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG8150.2124033108854742No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC8010.20875466505431273No Hit
ATCAACGGGTGTGTGTGTGTATGTGCCTGTGTATGTGTGCCTGTGTGTATGT7760.2022392260700957No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAAT7540.19650563976398475No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG7100.18503846715176284No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT6920.18034735108312658No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA6840.17826241060817713No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC6620.17252882430206617No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC6320.16471029752100577No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC6270.16340720972416237No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT5850.1524612722306778No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT5840.15220065467130914No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT5290.13786668890603174No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5240.13656360110918833No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC5240.13656360110918833No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT5200.1355211308717136No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCATA4740.12353272314075434No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATTGT4690.12222963534391093No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC4690.12222963534391093No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGAC4630.12066592998769886No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG4610.12014469486896148No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT4480.11675666659716866No Hit
ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4440.11571419635969392No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTACTC4420.11519296124095657No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT4380.11415049100348186No Hit
ATCAACGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT4330.11284740320663844No Hit
ATCAACGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCT4300.11206555052853241No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGATGA4250.11076246273168901No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT4200.10945937493484562No Hit
ATCAACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA4140.10789566957863353No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA4060.10581072910368408No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGCTC4050.10555011154431541No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC4040.10528949398494673No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTC4040.10528949398494673No Hit
ATCAACGGGAGCGAGGAACTGTACCACCGTTGTCGCCTCAGCAATCTGGAGG4030.10502887642557805No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG4000.104247023747472No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCGGT3950.10294393595062862No Hit
ATCAACGGGAAATCAATTCATAAAATTTCACGTCTATTTCATGATAGACGTG3940.10268331839125994No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCCTG3940.10268331839125994No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3890.10138023059441653No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3850.10033776035694181No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC3850.10033776035694181No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC3840.10007714279757314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG551.8189894E-1246.00000421
TTTACGT551.8189894E-1246.00000422
TAATCAG206.3061726E-446.024
TCGCCCC301.8581395E-646.040
CTTCGAC405.5952114E-946.018
CGGAATC301.8581395E-646.019
TAATAAC253.4128723E-546.042
AGCCGAA206.3061726E-446.038
GATACCG301.8581395E-646.042
CTCACCG253.4128723E-546.037
ACACGTT253.4128723E-546.029
ACACGGG253.4128723E-546.046
ACACGCA206.3061726E-446.041
TCACCGC253.4128723E-546.038
CATATCG253.4128723E-546.029
CGTATGG206.3061726E-446.011
GATCGCG206.3061726E-446.013
GGTCGGT405.5952114E-946.039
AAATAGG206.3061726E-446.046
ACGTTTC253.4128723E-546.031
Produced by FastQC (version 0.11.5)