##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383704
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801719554656714	31.0	31.0	34.0	30.0	34.0
2	32.23248389383483	33.0	31.0	34.0	31.0	34.0
3	32.69657600650501	34.0	31.0	34.0	31.0	34.0
4	36.23483466422034	37.0	37.0	37.0	35.0	37.0
5	36.44049319266935	37.0	37.0	37.0	35.0	37.0
6	36.53193086337385	37.0	37.0	37.0	35.0	37.0
7	36.56579550903822	37.0	37.0	37.0	35.0	37.0
8	36.670899964556014	37.0	37.0	37.0	35.0	37.0
9	38.63436138273252	39.0	39.0	39.0	39.0	39.0
10	37.39252392469195	39.0	37.0	39.0	34.0	39.0
11	37.643548151700266	39.0	37.0	39.0	35.0	39.0
12	37.26248097491817	39.0	37.0	39.0	35.0	39.0
13	37.17941173404499	39.0	37.0	39.0	35.0	39.0
14	37.837345975022416	40.0	37.0	40.0	34.0	40.0
15	37.920404791193214	40.0	37.0	40.0	34.0	40.0
16	38.00221003690345	40.0	37.0	40.0	35.0	40.0
17	37.893133769780874	40.0	37.0	40.0	34.0	40.0
18	37.76286668890603	40.0	36.0	40.0	34.0	40.0
19	37.58834674645039	40.0	36.0	40.0	33.0	40.0
20	37.51603840460355	39.0	36.0	40.0	33.0	40.0
21	37.483714008715054	39.0	36.0	40.0	33.0	40.0
22	37.54836540666764	39.0	36.0	40.0	33.0	40.0
23	37.43020140524988	39.0	36.0	40.0	33.0	40.0
24	37.492369117861685	39.0	36.0	40.0	34.0	40.0
25	37.538910201613746	39.0	36.0	40.0	34.0	40.0
26	37.50592383712445	39.0	36.0	40.0	34.0	40.0
27	37.44270583574839	39.0	35.0	40.0	34.0	40.0
28	37.27878260325668	39.0	35.0	40.0	33.0	40.0
29	37.24562944352939	39.0	35.0	40.0	33.0	40.0
30	37.04240247690928	39.0	35.0	40.0	32.0	40.0
31	37.07105998373746	39.0	35.0	40.0	33.0	40.0
32	36.93832224839981	39.0	35.0	40.0	33.0	40.0
33	36.73122771719867	38.0	35.0	40.0	32.0	40.0
34	36.239510143235414	38.0	35.0	40.0	31.0	40.0
35	36.17317254967371	38.0	35.0	40.0	31.0	40.0
36	36.11332954569147	38.0	35.0	40.0	30.0	40.0
37	36.14390259158101	38.0	35.0	40.0	31.0	40.0
38	36.346566624272874	38.0	35.0	40.0	31.0	40.0
39	36.33173487896921	38.0	35.0	40.0	31.0	40.0
40	36.16952911619373	38.0	35.0	40.0	31.0	40.0
41	36.121088130433876	38.0	35.0	40.0	31.0	40.0
42	35.99848581198007	38.0	35.0	40.0	31.0	40.0
43	35.91001396910118	38.0	35.0	40.0	31.0	40.0
44	35.818232283218315	38.0	35.0	40.0	31.0	40.0
45	35.67061067906511	37.0	34.0	40.0	30.0	40.0
46	35.39738704834977	37.0	34.0	40.0	30.0	40.0
47	35.20520244772012	37.0	34.0	40.0	29.0	40.0
48	34.99649730000208	36.0	34.0	40.0	28.0	40.0
49	34.957581886037154	36.0	34.0	40.0	29.0	40.0
50	35.41996955986907	36.0	34.0	40.0	30.0	40.0
51	35.59596459771073	36.0	35.0	40.0	31.0	40.0
52	35.29806569647437	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	6.0
14	13.0
15	18.0
16	24.0
17	41.0
18	80.0
19	105.0
20	177.0
21	318.0
22	465.0
23	697.0
24	972.0
25	1242.0
26	1700.0
27	2126.0
28	2836.0
29	3866.0
30	5240.0
31	7598.0
32	10861.0
33	17512.0
34	37553.0
35	28186.0
36	39372.0
37	70131.0
38	133198.0
39	19364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.7526843608615	1.0518524696119924	0.10502887642557805	0.09043429310093197
8	98.44098575985656	0.3236870087359006	0.11936284219085545	1.115964389216688
9	96.51215520296896	2.235838041823906	0.3523549402664554	0.8996518149406835
10	51.021620832725226	35.12577403415133	4.421898129808394	9.430707003315057
11	37.82915997748264	20.527020828555344	23.5978775305965	18.04594166336551
12	34.42419156433083	15.287304797448034	25.43392823634885	24.854575401872275
13	26.7774117548944	15.156996017763694	30.53916560682193	27.526426620519985
14	25.309353042970624	17.346183516460606	33.30145111857056	24.043012321998205
15	28.031764068135857	18.510883389279236	29.421376894689654	24.035975647895253
16	32.888372286971205	21.2114025394575	24.923378437545608	20.976846736025685
17	32.74920251026833	20.384984258699415	25.68255738798657	21.183255843045682
18	30.766945353710152	20.596084481788047	25.80009590726185	22.836874257239955
19	29.943393866105122	24.14152575943957	23.818359985822404	22.096720388632903
20	29.820903613201843	24.347413631340824	24.298417530179513	21.533265225277816
21	28.0679899088881	24.931718199445406	25.964545587223487	21.03574630444301
22	27.63223734962367	22.14936513562538	27.984592289890127	22.23380522486083
23	29.085440860663432	23.180628818047243	25.441225528011174	22.29270479327815
24	30.026530867543734	21.28202989804641	24.34663177866272	24.344807455747137
25	28.719533807309798	22.257260805204012	22.27524341680045	26.747961970685736
26	23.749296332589704	23.048235097887957	25.42220044617726	27.78026812334508
27	22.978128974417782	20.776953067989908	24.768832224839983	31.476085732752328
28	25.550163667827285	24.602558221962763	22.84573525425849	27.00154285595146
29	29.206888643329233	23.784219085545107	22.212695202551966	24.79619706857369
30	28.4216479369514	20.935669161645436	25.397441778037237	25.24524112336593
31	32.38277422179597	20.398536371786584	24.102954360653005	23.115735045764442
32	32.964211996747494	20.24138398348727	24.836853407835207	21.957550611930028
33	33.52193357379647	20.503304630652792	24.272616391802014	21.702145403748723
34	26.69948710464316	23.508485707733044	28.992661009528177	20.799366178095617
35	25.651022663302964	27.839428309321768	26.20196818380835	20.307580843566917
36	26.05445864520568	28.61893542939349	25.554594166336553	19.772011759064277
37	29.656453933240208	27.48941892708963	21.610929257969687	21.243197881700475
38	28.13601109188333	29.093780622563227	22.580426578821175	20.189781706732273
39	30.088818464232848	25.488918541375643	22.20227050017722	22.219992494214292
40	25.39092633905302	24.183485186497926	23.651304130267082	26.774284344181975
41	24.56633238121052	26.90276880095073	21.89057189917228	26.640326918666474
42	23.123553572545504	23.124856660342346	24.96820465775702	28.783385109355127
43	23.966390759543817	23.82982715843463	22.617694889810895	29.586087192210663
44	24.40813752267373	21.861382732522987	24.912171882492753	28.818307862310533
45	26.62938098117299	21.48974209286325	24.20641953172237	27.674457394241394
46	26.60331922523612	25.080791443404294	23.442810082772137	24.873079248587455
47	24.72322415195046	24.43758730688239	24.534797656526905	26.304390884640245
48	26.254873548360198	24.04457602735442	25.695848883514376	24.004701540771013
49	26.802431040593795	22.751912932885766	25.559806517523924	24.885849508996518
50	24.47094635448158	21.182213372808206	26.974959864895858	27.37188040781436
51	23.92964368367283	22.991420469945584	27.393251047682586	25.685684798699
52	22.331015574505347	23.198350812084314	27.966349060734313	26.504284552676022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	6.5
6	2.0
7	2.0
8	2.0
9	41.5
10	81.0
11	200.0
12	319.0
13	371.5
14	429.5
15	435.0
16	594.0
17	753.0
18	718.0
19	683.0
20	826.5
21	970.0
22	883.0
23	796.0
24	989.0
25	1182.0
26	1286.5
27	1391.0
28	2101.0
29	2811.0
30	3762.5
31	4714.0
32	5671.0
33	6628.0
34	6424.0
35	6220.0
36	8205.5
37	10191.0
38	10188.0
39	10204.5
40	10224.0
41	11356.0
42	12488.0
43	13483.0
44	14478.0
45	15404.5
46	16331.0
47	19238.5
48	22146.0
49	21462.5
50	20779.0
51	21638.0
52	22497.0
53	22739.0
54	22981.0
55	29100.0
56	35219.0
57	34007.5
58	32796.0
59	33021.0
60	33246.0
61	34675.0
62	36104.0
63	27878.5
64	16558.0
65	13463.0
66	10205.5
67	6948.0
68	6298.0
69	5648.0
70	5155.5
71	4663.0
72	3690.5
73	2718.0
74	2217.5
75	1717.0
76	1449.0
77	1181.0
78	800.5
79	420.0
80	291.0
81	162.0
82	97.5
83	33.0
84	21.0
85	9.0
86	5.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	383704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.574296853824823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.29925568097796	7.116423076121177
2	9.599743678217578	2.0302107874820177
3	3.258244195790408	1.033609240456185
4	1.6094050377088776	0.6807330650709922
5	0.897126238477843	0.4743239580509977
6	0.6851678414748361	0.43471008902695824
7	0.5225020949376448	0.38675645810312115
8	0.5348252575540987	0.4524320830640285
9	0.4165228964361414	0.3963993077997623
>10	11.073593927145463	30.442476492296144
>50	2.548430029082664	18.303953047140503
>100	1.4960319416375019	29.94365448366449
>500	0.04189875289594321	2.936378041406918
>1k	0.014787795139744665	3.6814836436419736
>5k	0.002464632523290777	1.6864562266747283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6471	1.6864562266747283	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3992	1.0403852969997707	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3276	0.8537831244917958	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2104	0.5483393449117028	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2100	0.547296874674228	No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATGTA	1438	0.3747680503721619	No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1216	0.3169109521923149	No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	848	0.22100369034464065	No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	815	0.2124033108854742	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	801	0.20875466505431273	No Hit
ATCAACGGGTGTGTGTGTGTATGTGCCTGTGTATGTGTGCCTGTGTGTATGT	776	0.2022392260700957	No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAAT	754	0.19650563976398475	No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	710	0.18503846715176284	No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT	692	0.18034735108312658	No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	684	0.17826241060817713	No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC	662	0.17252882430206617	No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC	632	0.16471029752100577	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	627	0.16340720972416237	No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	585	0.1524612722306778	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	584	0.15220065467130914	No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	529	0.13786668890603174	No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	524	0.13656360110918833	No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	524	0.13656360110918833	No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	520	0.1355211308717136	No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCATA	474	0.12353272314075434	No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATTGT	469	0.12222963534391093	No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	469	0.12222963534391093	No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGAC	463	0.12066592998769886	No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG	461	0.12014469486896148	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	448	0.11675666659716866	No Hit
ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	444	0.11571419635969392	No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTACTC	442	0.11519296124095657	No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT	438	0.11415049100348186	No Hit
ATCAACGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	433	0.11284740320663844	No Hit
ATCAACGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCT	430	0.11206555052853241	No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGATGA	425	0.11076246273168901	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	420	0.10945937493484562	No Hit
ATCAACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	414	0.10789566957863353	No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	406	0.10581072910368408	No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGCTC	405	0.10555011154431541	No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC	404	0.10528949398494673	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTC	404	0.10528949398494673	No Hit
ATCAACGGGAGCGAGGAACTGTACCACCGTTGTCGCCTCAGCAATCTGGAGG	403	0.10502887642557805	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG	400	0.104247023747472	No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCGGT	395	0.10294393595062862	No Hit
ATCAACGGGAAATCAATTCATAAAATTTCACGTCTATTTCATGATAGACGTG	394	0.10268331839125994	No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCCTG	394	0.10268331839125994	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	389	0.10138023059441653	No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	385	0.10033776035694181	No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	385	0.10033776035694181	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	384	0.10007714279757314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0026061755936868003	0.0	0.0	0.0	0.0
8	0.0033880282717928404	0.0	0.0	0.0	0.0
9	0.01589767112148948	0.0	0.0	0.0	0.0
10	0.019546316952651	0.0	0.0	0.0	0.0
11	0.02762546129308008	0.0	0.0	0.0	0.0
12	0.03361966515855972	0.0	0.0	0.0	0.0
13	0.04117757438025144	0.0	0.0	0.0	0.0
14	0.0495173362800492	0.0	0.0	0.0	0.0
15	0.05629339282363489	0.0	0.0	0.0	0.0
16	0.149855096636991	0.0	0.0	0.0	0.0
17	0.15871609365552614	0.0	0.0	0.0	0.0
18	0.16392844484289973	0.0	0.0	0.0	0.0
19	0.17695932281133372	0.0	0.0	0.0	0.0
20	0.1879052603048183	0.0	0.0	0.0	0.0
21	0.19650563976398475	0.0	0.0	0.0	0.0
22	0.2087546650543127	0.0	0.0	0.0	0.0
23	0.21865813231032255	0.0	0.0	0.0	0.0
24	0.2283009820069637	0.0	0.0	0.0	0.0
25	0.23403456831307465	0.0	0.0	0.0	0.0
26	0.24211371265350373	0.0	0.0	0.0	0.0
27	0.2567082959781498	0.0	0.0	0.0	0.0
28	0.2673936159122657	0.0	0.0	0.0	0.0
29	0.27599399537143215	0.0	0.0	0.0	0.0
30	0.28615808018681066	0.0	0.0	0.0	0.0
31	0.2968434001209265	0.0	0.0	0.0	0.0
32	0.3080499551737798	0.0	0.0	0.0	0.0
33	0.31508662927673414	0.0	0.0	0.0	0.0
34	0.32499009653274397	0.0	0.0	0.0	0.0
35	0.3369785042637033	0.0	0.0	0.0	0.0
36	0.3544398807414048	0.0	0.0	0.0	0.0
37	0.37216187477847507	0.0	0.0	0.0	0.0
38	0.38675645810312115	0.0	0.0	0.0	0.0
39	0.39509622000291894	0.0	0.0	0.0	0.0
40	0.4029147467839793	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	55	1.8189894E-12	46.000004	21
TTTACGT	55	1.8189894E-12	46.000004	22
TAATCAG	20	6.3061726E-4	46.0	24
TCGCCCC	30	1.8581395E-6	46.0	40
CTTCGAC	40	5.5952114E-9	46.0	18
CGGAATC	30	1.8581395E-6	46.0	19
TAATAAC	25	3.4128723E-5	46.0	42
AGCCGAA	20	6.3061726E-4	46.0	38
GATACCG	30	1.8581395E-6	46.0	42
CTCACCG	25	3.4128723E-5	46.0	37
ACACGTT	25	3.4128723E-5	46.0	29
ACACGGG	25	3.4128723E-5	46.0	46
ACACGCA	20	6.3061726E-4	46.0	41
TCACCGC	25	3.4128723E-5	46.0	38
CATATCG	25	3.4128723E-5	46.0	29
CGTATGG	20	6.3061726E-4	46.0	11
GATCGCG	20	6.3061726E-4	46.0	13
GGTCGGT	40	5.5952114E-9	46.0	39
AAATAGG	20	6.3061726E-4	46.0	46
ACGTTTC	25	3.4128723E-5	46.0	31
>>END_MODULE